scholarly journals Cell Sheet Engineering Toward Lung Epithelial Cell Culture and Regenerative Medicine.

MEMBRANE ◽  
2002 ◽  
Vol 27 (5) ◽  
pp. 216-220
Author(s):  
Masayuki Yamato
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Lung Epithelial Cell ◽  
Epithelial Cell Culture ◽  
Cell Sheet Engineering ◽  
Lung Epithelial

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

2007 ◽  
Vol 32 (8-9) ◽  
pp. 1123-1133 ◽  
Author(s):  
Masayuki Yamato ◽  
Yoshikatsu Akiyama ◽  
Jun Kobayashi ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Cell

Application for regenerative medicine of epithelial cell culture-vistas of cultured epithelium

2006 ◽  
Vol 46 (3) ◽  
pp. 129-134 ◽  
Author(s):  
Hajime Inoue ◽  
Hideo Oshima ◽  
Kyo-Ichi Matsuzaki ◽  
Norio Kumagai
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Regenerative Medicine ◽  
Epithelial Cell Culture ◽  
Cultured Epithelium

scholarly journals Bactericidal Activity of Ceragenin CSA-13 in Cell Culture and in an Animal Model of Peritoneal Infection

2015 ◽  
Vol 59 (10) ◽  
pp. 6274-6282 ◽  
Author(s):  
Robert Bucki ◽  
Katarzyna Niemirowicz ◽  
Urszula Wnorowska ◽  
Fitzroy J. Byfield ◽  
Ewelina Piktel ◽  
...  
Keyword(s):  
Cell Culture ◽  
Animal Model ◽  
Epithelial Cell ◽  
Bactericidal Activity ◽  
Human Lung ◽  
Cationic Lipids ◽  
Lung Epithelial Cell ◽  
Human Lung Epithelial Cell ◽  
Lung Epithelial ◽  
Peritoneal Infection

ABSTRACTCeragenins constitute a novel family of cationic antibiotics characterized by a broad spectrum of antimicrobial activities, which have mostly been assessedin vitro. Using a polarized human lung epithelial cell culture system, we evaluated the antibacterial activities of the ceragenin CSA-13 against two strains ofPseudomonas aeruginosa(PAO1 and Xen5). Additionally, the biodistribution and bactericidal activity of a CSA-13–IRDye 800CW derivate were assessed using an animal model of peritoneal infection after PAO1 challenge. In cell culture, CSA-13 bactericidal activities against PAO1 and Xen5 were higher than the activities of the human cathelicidin peptide LL-37. Increased CSA-13 activity was observed in polarized human lung epithelial cell cultures subjected to butyric acid treatment, which is known to increase endogenous LL-37 production. Eight hours after intravenous or intraperitoneal injection, the greatest CSA-13–IRDye 800CW accumulation was observed in mouse liver and kidneys. CSA-13–IRDye 800CW administration resulted in decreased bacterial outgrowth from abdominal fluid collected from animals subjected to intraperitoneal PAO1 infection. These observations indicate that CSA-13 may synergistically interact with antibacterial factors that are naturally present at mucosal surfaces and it maintains its antibacterial activity in the infected abdominal cavity. Cationic lipids such as CSA-13 represent excellent candidates for the development of new antibacterial compounds.

Sign in / Sign up

Export Citation Format

Share Document