scholarly journals 3D Cell Culture Models Demonstrate a Role for FGF and WNT Signaling in Regulation of Lung Epithelial Cell Fate and Morphogenesis

2020 ◽  
Vol 8 ◽  
Author(s):  
Anas Rabata ◽  
Radek Fedr ◽  
Karel Soucek ◽  
Ales Hampl ◽  
Zuzana Koledova
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Wnt Signaling ◽  
Cell Fate ◽  
3D Cell Culture ◽  
Lung Epithelial Cell ◽  
Lung Epithelial ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals 3D cell culture models demonstrate a role for FGF and WNT signaling in regulation of lung epithelial cell fate and morphogenesis

2020 ◽  
Author(s):  
Anas Rabata ◽  
Radek Fedr ◽  
Karel Soucek ◽  
Ales Hampl ◽  
Zuzana Koledova
Keyword(s):  
Cell Culture ◽  
Wnt Signaling ◽  
Lung Development ◽  
Ex Vivo ◽  
3D Cell Culture ◽  
Epithelial Morphogenesis ◽  
Fgf Signaling ◽  
Lung Epithelial ◽  
Culture Models ◽  
Cell Culture Models

AbstractFGF signaling plays an essential role in lung development, homeostasis, and regeneration. Several FGF ligands were detected in the developing lungs, however, their roles have not been fully elucidated. We employed mouse 3D cell culture models and imaging to ex vivo study of a) the role of FGF ligands in lung epithelial morphogenesis and b) the interplay of FGF signaling with epithelial growth factor (EGF) and WNT signaling pathways. In non-adherent conditions, FGF signaling promoted formation of lungospheres from lung epithelial stem/progenitor cells (LSPCs). Based on their architecture, we defined three distinct phenotypes of lungospheres. Ultrastructural and immunohistochemical analyses showed that LSPCs produced more differentiated lung cell progeny. In 3D extracellular matrix, FGF2, FGF7, FGF9, and FGF10 promoted lung organoid formation with similar efficiency. However, FGF9 showed reduced capacity to promote lung organoid formation, suggesting that FGF9 has a reduced ability to sustain LSPCs survival and/or initial divisions. Analysis of lung organoid phenotypes revealed that FGF7 and FGF10 produce bigger organoids and induce organoid branching with higher frequency than FGF2 and FGF9. Higher FGF concentration and/or the use of FGF2 with increased stability and affinity to FGF receptors both increased lung organoid and lungosphere formation efficiency, respectively, suggesting that the level of FGF signaling is a crucial driver of LSPC survival and differentiation, and also lung epithelial morphogenesis. EGF signaling played a supportive but nonessential role in FGF-induced lung organoid formation. Moreover, analysis of tissue architecture and cell type composition confirmed that the lung organoids contained alveolar-like regions with cells expressing alveolar type I and type II cell markers, as well as airway-like structures with club cells and ciliated cells. WNT signaling enhanced the efficiency of lung organoid formation, but in the absence of FGF10 signaling, the organoids displayed limited branching and less differentiated phenotype. In summary, we present lung 3D cell culture models as useful tools to study the role and interplay of signaling pathways in lung development and we reveal roles for FGF ligands in regulation of mouse lung morphogenesis ex vivo.

scholarly journals Conjunctival melanoma and electrochemotherapy: preliminary results using 2D and 3D cell culture models in vitro

2018 ◽  
Vol 97 (4) ◽  
pp. e632-e640 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Periklis Katopodis ◽  
Helen Kalirai ◽  
Berthold Seitz ◽  
Arne Viestenz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Conjunctival Melanoma ◽  
Preliminary Results ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

2010 ◽  
Vol 8 (11) ◽  
pp. 791-801 ◽  
Author(s):  
Jennifer Barrila ◽  
Andrea L. Radtke ◽  
Aurélie Crabbé ◽  
Shameema F. Sarker ◽  
Melissa M. Herbst-Kralovetz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Host Pathogen Interactions ◽  
Rotating Wall ◽  
Rotating Wall Vessel ◽  
Host Pathogen ◽  
Culture Models ◽  
Cell Culture Models

Length vs. stiffness: which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

2018 ◽  
Vol 6 (25) ◽  
pp. 4223-4231 ◽  
Author(s):  
Jiacheng Zhao ◽  
Hongxu Lu ◽  
Yin Yao ◽  
Sylvia Ganda ◽  
Martina H. Stenzel
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dominant Role ◽  
3D Cell Culture ◽  
Culture Models ◽  
Nano Rods ◽  
2D And 3D ◽  
Cell Culture Models

Internalization of rod-like micelles by breast cancer cells is significantly affected by the stiffness of nano-rods.

Human meibomian gland epithelial cell culture models: Current progress, challenges, and future directions

2021 ◽  
Author(s):  
Minh Anh Thu Phan ◽  
Michele C. Madigan ◽  
Fiona Stapleton ◽  
Mark Willcox ◽  
Blanka Golebiowski
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Meibomian Gland ◽  
Epithelial Cell Culture ◽  
Future Directions ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Untargeted Metabolite Mapping in 3D Cell Culture Models Using High Spectral Resolution FT-ICR Mass Spectrometry Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 9522-9529 ◽  
Author(s):  
Lulu H. Tucker ◽  
Gregory R. Hamm ◽  
Rebecca J. E. Sargeant ◽  
Richard J. A. Goodwin ◽  
C. Logan Mackay ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Spectral Resolution ◽  
Mass Spectrometry Imaging ◽  
3D Cell Culture ◽  
High Spectral Resolution ◽  
Culture Models ◽  
Cell Culture Models ◽  
Ft Icr

The surface charge of liposomal adjuvants is decisive for their interactions with the Calu-3 and A549 airway epithelial cell culture models

2014 ◽  
Vol 87 (3) ◽  
pp. 480-488 ◽  
Author(s):  
Pall Thor Ingvarsson ◽  
Ida Svahn Rasmussen ◽  
Michelle Viaene ◽  
Przemyslaw Jedrzej Irlik ◽  
Hanne Mørck Nielsen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Surface Charge ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Epithelial Cell Culture ◽  
Culture Models ◽  
Cell Culture Models
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