scholarly journals Standardization method for molecular weight cut-off evaluation of ultrafiltration membranes II. Measurment of molecular weight cut-off using GPC analysis method.

MEMBRANE ◽  
1990 ◽  
Vol 15 (2) ◽  
pp. 62-71 ◽  
Author(s):  
Haruhiko Ohya ◽  
Akihito Haio ◽  
Youich Negishi ◽  
Kanji Matsumoto
Keyword(s):  
Molecular Weight ◽  
Standardization Method ◽  
Analysis Method ◽  
Ultrafiltration Membranes

scholarly journals How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake

2019 ◽  
Vol 20 (8) ◽  
pp. 1939 ◽  
Author(s):  
Loïc Henaux ◽  
Jacinthe Thibodeau ◽  
Geneviève Pilon ◽  
Tom Gill ◽  
André Marette ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Glucose Uptake ◽  
Membrane Separation ◽  
Protein Hydrolysate ◽  
Biological Response ◽  
Ultrafiltration Membranes ◽  
Selective Membrane ◽  
Insulin Control ◽  
By Products ◽  
Different Molecular Weight

The valorization of by-products from natural organic sources is an international priority to respond to environmental and economic challenges. In this context, electrodialysis with filtration membrane (EDFM), a green and ultra-selective process, was used to separate peptides from salmon frame protein hydrolysate. For the first time, the simultaneous separation of peptides by three ultrafiltration membranes of different molecular-weight exclusion limits (50, 20, and 5 kDa) stacked in an electrodialysis system, allowed for the generation of specific cationic and anionic fractions with different molecular weight profiles and bioactivity responses. Significant decreases in peptide recovery, yield, and molecular weight (MW) range were observed in the recovery compartments depending on whether peptides had to cross one, two, or three ultrafiltration membranes. Moreover, the Cationic Recovery Compartment 1 fraction demonstrated the highest increase (42%) in glucose uptake on L6 muscle cells. While, in the anionic configuration, both Anionic Recovery Compartment 2 and Anionic Recovery Compartment 3 fractions presented a glucose uptake response in basal condition similar to the insulin control. Furthermore, Cationic Recovery Compartment 3 was found to contain inhibitory peptides. Finally, LC-MS analyses of the bioassay-guided bioactive fractions allowed us to identify 11 peptides from salmon by-products that are potentially responsible for the glucose uptake improvement.

Liquid–liquid displacement porometry to estimate the molecular weight cut-off of ultrafiltration membranes

Desalination ◽  
2011 ◽  
Vol 268 (1-3) ◽  
pp. 174-181 ◽  
Author(s):  
José Ignacio Calvo ◽  
René Israel Peinador ◽  
Pedro Prádanos ◽  
Laura Palacio ◽  
Aldo Bottino ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Ultrafiltration Membranes ◽  
Liquid Displacement
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