Novel functions for P70 S6 kinase : regulation of microRNA biogenesis and multicellular spheroid formation of ovarian cancer cells

2016 ◽  
Author(s):  
So-ngo Lam
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
S6 Kinase ◽  
P70 S6 Kinase ◽  
Microrna Biogenesis ◽  
Kinase Regulation

scholarly journals A novel p70 S6 kinase-microRNA biogenesis axis mediates multicellular spheroid formation in ovarian cancer progression

Oncotarget ◽  
2016 ◽  
Vol 7 (25) ◽  
pp. 38064-38077 ◽  
Author(s):  
Sophia So Ngo Lam ◽  
Carman Ka Man Ip ◽  
Abby Sin Chi Mak ◽  
Alice Sze Tsai Wong
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Multicellular Spheroid ◽  
Spheroid Formation ◽  
S6 Kinase ◽  
P70 S6 Kinase ◽  
Microrna Biogenesis

scholarly journals p70 S6 Kinase Promotes Epithelial to Mesenchymal Transition through Snail Induction in Ovarian Cancer Cells

2008 ◽  
Vol 68 (16) ◽  
pp. 6524-6532 ◽  
Author(s):  
Yuen L. Pon ◽  
Hong Y. Zhou ◽  
Annie N.Y. Cheung ◽  
Hextan Y.S. Ngan ◽  
Alice S.T. Wong
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Epithelial To Mesenchymal Transition ◽  
Ovarian Cancer Cells ◽  
S6 Kinase ◽  
Mesenchymal Transition ◽  
P70 S6 Kinase

scholarly journals CD83, a Novel MAPK Signaling Pathway Interactor, Determines Ovarian Cancer Cell Fate

Cancers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2269
Author(s):  
Aalia Batool ◽  
Hao Liu ◽  
Yi-Xun Liu ◽  
Su-Ren Chen
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Mapk Signaling ◽  
Migration And Invasion ◽  
Integrated Analysis ◽  
Ovarian Cancer Cells ◽  
Spheroid Formation ◽  
Regulation Of Proliferation

Ovarian cancer is a leading cause of death from gynecologic malignancies worldwide. Although CD83 is widely described as a solid marker for mature dendritic cells, emerging pieces of evidence indicate the expression of membrane protein CD83 by various tumor cells, including ovarian cancer cells. However, the potential role of CD83 in ovarian cancer cell properties and development remains absolutely unknown. By using human CD83 stable overexpression and knockdown sublines of several ovarian cancer cells, we observed that CD83 advanced the growth proliferation, colony formation ability, spheroid formation, and in vivo tumorigenicity of ovarian cancer cells; surprisingly, CD83 limited their migration and invasion potentials. Positive regulation of proliferation/stemness factors (e.g., cyclin-CDKs and KIT/CD44) but negative regulation of matrix metallopeptidases (e.g., MMP1 and 7) by CD83 were revealed by the integrated analysis of transcriptome and proteome. Furthermore, immunoprecipitation-mass spectrometry (IP-MS) and co-immunoprecipitation (Co-IP) first identified the association of CD83 with MAP3K7 (also known as TAK1) and MAP3K7-binding protein TAB1 on the cell membrane. Moreover, CD83 functions through the activation of MAP3K7-MEK1/2-ERK1/2 cascades to further regulate downstream FOXO1/p21/CDK2/CCNB1 and STAT3/DKK1 signaling pathways, thus activating proliferation and spheroid formation of ovarian cancer cells, respectively. Collectively, our findings define a CD83-MAPK pathway in the regulation of proliferation and stemness in ovarian cancer cells, with potential therapeutic applications in blocking their progression.

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