Exploring microbial community structures and functions of activated sludge by high-throughput sequencing

2012 ◽  
Author(s):  
Lin Ye
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Community Structures ◽  
Microbial Community Structures

scholarly journals Changes in the Soil Microbiome in Eggplant Monoculture Revealed by High-Throughput Illumina MiSeq Sequencing as Influenced by Raw Garlic Stalk Amendment

2019 ◽  
Vol 20 (9) ◽  
pp. 2125 ◽  
Author(s):  
Muhammad Imran Ghani ◽  
Ahmad Ali ◽  
Muhammad Jawaad Atif ◽  
Muhammad Ali ◽  
Bakht Amin ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Soil Microbial Communities ◽  
Illumina Miseq ◽  
Soil Microbiome ◽  
Plant Residues ◽  
Illumina Miseq Sequencing ◽  
Miseq Sequencing ◽  
Community Structures ◽  
Microbial Community Structures

The incorporation of plant residues into soil can be considered a keystone sustainability factor in improving soil structure function. However, the effects of plant residue addition on the soil microbial communities involved in biochemical cycles and abiotic stress phenomena are poorly understood. In this study, experiments were conducted to evaluate the role of raw garlic stalk (RGS) amendment in avoiding monoculture-related production constraints by studying the changes in soil chemical properties and microbial community structures. RGS was applied in four different doses, namely the control (RGS0), 1% (RGS1), 3% (RGS2), and 5% (RGS3) per 100 g of soil. The RGS amendment significantly increased soil electrical conductivity (EC), N, P, K, and enzyme activity. The soil pH significantly decreased with RGS application. High-throughput Illumina MiSeq sequencing revealed significant alterations in bacterial community structures in response to RGS application. Among the 23 major taxa detected, Anaerolineaceae, Acidobacteria, and Cyanobacteria exhibited an increased abundance level. RGS2 increased some bacteria reported to be beneficial including Acidobacteria, Bacillus, and Planctomyces (by 42%, 64%, and 1% respectively). Furthermore, internal transcribed spacer (ITS) fungal regions revealed significant diversity among the different treatments, with taxa such as Chaetomium (56.2%), Acremonium (4.3%), Fusarium (4%), Aspergillus (3.4%), Sordariomycetes (3%), and Plectosphaerellaceae (2%) showing much abundance. Interestingly, Coprinellus (14%) was observed only in RGS-amended soil. RGS treatments effectively altered soil fungal community structures and reduced certain known pathogenic fungal genera, i.e., Fusarium and Acremonium. The results of the present study suggest that RGS amendment potentially affects the microbial community structures that probably affect the physiological and morphological attributes of eggplant under a plastic greenhouse vegetable cultivation system (PGVC) in monoculture.

Activated sludge microbial community responses to single-walled carbon nanotubes: community structure does matter

2015 ◽  
Vol 71 (8) ◽  
pp. 1235-1240 ◽  
Author(s):  
Qiao Ma ◽  
Yuanyuan Qu ◽  
Wenli Shen ◽  
Jingwei Wang ◽  
Zhaojing Zhang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Microbial Community ◽  
Activated Sludge ◽  
High Throughput Sequencing ◽  
Alpha Diversity ◽  
Single Walled Carbon Nanotubes ◽  
Community Structures ◽  
Single Walled Carbon ◽  
Microbial Responses ◽  
Walled Carbon Nanotubes

The ecological effects of carbon nanotubes (CNTs) have been a worldwide research focus due to their extensive release and accumulation in environment. Activated sludge acting as an important gathering place will inevitably encounter and interact with CNTs, while the microbial responses have been rarely investigated. Herein, the activated sludges from six wastewater treatment plants were acclimated and treated with single-walled carbon nanotubes (SWCNTs) under identical conditions. Illumina high-throughput sequencing was applied to in-depth analyze microbial changes and results showed SWCNTs differently perturbed the alpha diversity of the six groups (one increase, two decrease, three no change). Furthermore, the microbial community structures were shifted, and specific bacterial performance in each group was different. Since the environmental and operational factors were identical in each group, it could be concluded that microbial responses to SWCNTs were highly depended on the original community structures.

Effects of Hg2+ on Microbial Community Structures of Aerobic Activated Sludge

2011 ◽  
Vol 343-344 ◽  
pp. 333-339
Author(s):  
Ning Dai ◽  
Jin Sheng Wang ◽  
Yan Guo Teng ◽  
Jie Su
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Nested Pcr ◽  
Operation Time ◽  
Community Structures ◽  
Microbial Species ◽  
Obvious Change ◽  
Microbial Community Structures ◽  
Operation Period

The effects of Hg2+ on microbial community structures of aerobic activated sludge were investigated by nested PCR-DGGE techniques. The results showed that the microbial community of each group might have obvious change compared with the group without Hg2+ in the inflow, especially when the Hg2+ concentration was 46.87mg/L in the inflow, and the microbial community changed obviously with the extend of operation time. The variance of microbial species become obvious in each activated sludge group with the extend of operation time. Compared with the group without Hg2+ in the inflow, the dominant microbial species of each activated sludge group changed, and the dominant microbial species of the group whose concentration of Hg2+ increased gradually in the inflow had the biggest changes after 60 days. Hg2+ in the inflow may stimulate the growth of Actinomycetes after an operation period of 30 days.

Linking nitrification characteristic and microbial community structures in integrated fixed film activated sludge reactor by high-throughput sequencing

2016 ◽  
Vol 74 (6) ◽  
pp. 1354-1364 ◽  
Author(s):  
Bin Dong ◽  
Jie Tan ◽  
Yang Yang ◽  
Zishan Pang ◽  
Zhongtian Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Ammonia Removal ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Nitrifying Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Fixed Film

The primary goal of this study is to investigate ammonia removal, abundance of nitrifying bacteria and microbial community structures in a laboratory-scale integrated fixed film activated sludge (IFAS) reactor. The results of Illumina MiSeq sequencing based on 16S rRNA genes showed Proteobacteria and Bacteroidetes were the dominant phyla in both biofilm and suspended sludge samples in the IFAS reactor. The dominant ammonia-oxidizing bacteria (AOB) species was Nitrosomonas and the dominant nitrite-oxidizing bacteria species was Nitrospira. The contribution of biofilm to ammonia removal increased from 4.0 ± 0.9% to 37.0 ± 2% when the temperature decreased from 25 °C to 10 °C. The real-time polymerase chain reaction (PCR) result showed the abundance of AOB in suspended sludge was higher than that in biofilm at the same time. However, nitrification is more dependent on attached growth than on suspended growth in the IFAS reactor at 15 °C and 10 °C and the abundance of AOB in biofilm was also higher than that in suspended sludge. The more robust ammonia removal rate at low temperatures by biofilm contributed to the relatively stable ammonia removal, and biofilm attached on carriers in the IFAS reactor is advantageous for nitrification in low-temperature environment.

Application of molecular methods to microbial community analysis of activated sludge

2000 ◽  
Vol 42 (3-4) ◽  
pp. 17-22 ◽  
Author(s):  
M. Onuki ◽  
H. Satoh ◽  
T. Mino ◽  
T. Matsuo
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wastewater Treatment Plants ◽  
Community Analysis ◽  
Molecular Methods ◽  
Microbial Community Analysis ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Gradient Gel Electrophoresis

In the last decade, molecular biology has made significant progress, and innovative molecular methods have become available to analyze microbial community structures. Among them, we applied the FISH (Fluorescent in situ Hybridization) method to analyze activated sludge in wastewater treatment plants (WWTPs). As a result, domain- or division-level community structures in activated sludge were determined successfully without cultivation. We also applied the PCR (polymerase chain reaction) -DGGE (Denaturing Gradient Gel Electrophoresis) method for laboratory nitrifying sludge in order to investigate more detailed microbial community structure. By this method, genus- or species-level community structures were characterized well. This method was also found to be powerful for monitoring the change of microbial community structures. For example, the behavior of Nitrosomonas group was successfully detected in the reactor with nitrification by the PCR-DGGE method.

Ammonium removal by native microbes and activated sludge within the Jialu River basin and the associated microbial community structures

2017 ◽  
Vol 76 (12) ◽  
pp. 3358-3367 ◽  
Author(s):  
Guangyi Zhang ◽  
Luji Yu ◽  
Panlong Liu ◽  
Zheng Fan ◽  
Tingmei Li ◽  
...  
Keyword(s):  
Activated Sludge ◽  
River Basin ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Cost Effective ◽  
Native Community ◽  
Ammonium Removal ◽  
Microbial Community Structures ◽  
Removal Efficiencies ◽  
Microbial Sources

Abstract To explore the availability of native microbes and activated sludge for ammonium removal, the native microbes and activated sludge in Jialu River basin were investigated in terms of ammonium-removing activities and their microbial communities using spectrophotometry and high-throughput sequencing. NH4+-N and total nitrogen (TN) in the targeted river ranged from 2.45 ± 1.76 to 8.56 ± 2.54 mg/L and from 3.42 ± 2.79 to 13.49 ± 5.06 mg/L, respectively. Both the native microbes and activated sludge had strong ammonium-removing activities with the removal efficiencies of more than 94%. High-throughput sequencing results indicated that, after five batches of operation, the class Gammaproteobacteria (28.55%), Alphaproteobacteria (14.55%), Betaproteobacteria (13.89%), Acidobacteria (8.82%) and Bacilli (7.04%) were dominated in native community, and there was a predominance of Gammaproteobacteria (21.57%), Betaproteobacteria (16.33%), Acidobacteria (12.41%), Alphaproteobacteria (10.01%), Sphingobacteriia (6.92%) and Bacilli (6.66%) in activated sludge. These two microbial sources were able to remove ammonium, while activated sludge was more cost-effective.

Sign in / Sign up

Export Citation Format

Share Document