Investigation of the gap junction intercellular communication between embryonic stem cells and connexin-43 over-expressing human foreskin fibroblasts and HeLa cells

2011 ◽  
Author(s):  
Yee-kwan Li
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gap Junction ◽  
Hela Cells ◽  
Intercellular Communication ◽  
Connexin 43 ◽  
Embryonic Stem ◽  
Human Foreskin Fibroblasts ◽  
Gap Junction Intercellular Communication

Connexin 43 is involved in early differentiation of human embryonic stem cells

2019 ◽  
Vol 105 ◽  
pp. 33-44 ◽  
Author(s):  
Qian Peng ◽  
Chaomin Yue ◽  
Andy Chun Hang Chen ◽  
Kai Chuen Lee ◽  
Sze Wan Fong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Connexin 43 ◽  
Embryonic Stem ◽  
Early Differentiation

Gap Junction Intercellular Communication is Likely to be Involved in Regulation of Stroma-dependent Proliferation of Hemopoietic Stem Cells

Hematology ◽  
2000 ◽  
Vol 5 (2) ◽  
pp. 133-147 ◽  
Author(s):  
Rob E. Ploemacher ◽  
Angelique E. M. Mayen ◽  
Alexandra E. De Koning ◽  
Tibor Krenacs ◽  
Martin Rosendaal
Keyword(s):  
Stem Cells ◽  
Gap Junction ◽  
Intercellular Communication ◽  
Hemopoietic Stem Cells ◽  
Gap Junction Intercellular Communication

scholarly journals Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT

2020 ◽  
Author(s):  
C. Aban ◽  
A. Lombardi ◽  
G. Neiman ◽  
M.C. Biani ◽  
A. La Greca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Connexin 43 ◽  
Cancer Metastasis ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Mesenchymal Transition ◽  
E Cadherin

Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological events including embryonic stem cell differentiation. During early stages of differentiation, human embryonic stem cells pass through EMT where deeper morphological, molecular and biochemical changes occur. Though initially considered as a decision between two states, EMT process is now regarded as a fluid transition where cells exist on a spectrum of intermediate states. In this work, using a CRISPR interference system in human embryonic stem cells, we describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal. Our results suggest that the decrease and delocalization of E-cadherin causes an incomplete EMT where cells retain their undifferentiated state while expressing several characteristics of a mesenchymal-like pheno-type. Namely, we found that E-cadherin downregulation induces SNAI1 and SNAI2 upregulation, promotes MALAT1 and LINC-ROR downregulation, modulates the expression of tight junction occludin 1 and gap junction connexin 43, increases human embryonic stem cells migratory capacity and delocalize b-catenin. Altogether, we believe our results provide a useful tool to model the molecular events of an unstable intermediate state and further identify multiple layers of molecular changes that occur during partial EMT.

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