Study of the function of Kinesin-1 (KIF5B) in long bone development

2009 ◽  
Author(s):  
Guixia Zhu
Keyword(s):  
Bone Development ◽  
Long Bone

Isolation, Culture and Identification of Bovine Skeletal Stem Cells

2021 ◽  
Vol 11 (12) ◽  
pp. 2337-2345
Author(s):  
Junhui Lai ◽  
Qin Yang ◽  
Ruining Liang ◽  
Weijun Guan ◽  
Xiuxia Li
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Bone Formation ◽  
Growth Plate ◽  
Bone Development ◽  
Long Bone ◽  
Biological Characterization ◽  
Self Renewal ◽  
And Cartilage

The growth plate is essential in long bone formation and contains a wealth of skeletal stem cells (SSCs). Though the origin and the mechanism for SSCs generation remain uncertain, recent studies demonstrate the transition from cartilage to bone that in the lineage for bone development. SSCs possesses the ability to differentiate into bone and cartilage in vitro. In this research, we aimed to isolate and culture the skeletal stem cells from bovine cattle and then studied its biological characterization. The results showed that these bovine SSCs are positive for PDPN+CD73+CD164+CD90+CD44+ cell surface bio-markers, they are capable of self-renewal and differentiation. Our dates proved that SSCs exists in bovine’s long bone.

Long-bone development and life-history traits of the Devonian tristichopterid Hyneria lindae

2018 ◽  
Vol 109 (1-2) ◽  
pp. 75-86 ◽  
Author(s):  
Viktoriia KAMSKA ◽  
Edward B. DAESCHLER ◽  
Jason P. DOWNS ◽  
Per E. AHLBERG ◽  
Paul TAFFOREAU ◽  
...  
Keyword(s):  
Life History ◽  
Limb Development ◽  
Life History Traits ◽  
Bone Development ◽  
Bone Histology ◽  
Long Bone ◽  
General Character ◽  
Appendicular Skeleton ◽  
Predatory Behaviour ◽  
Large Size

ABSTRACTHyneria lindae is one of the largest Devonian sarcopterygians. It was found in the Catskill Formation (late Famennian) of Pennsylvania, USA. The current study focuses on the palaeohistology of the humerus of this tristichopterid and supports a low ossification rate and a late ossification onset in the appendicular skeleton. In addition to anatomical features, the large size of the cell lacunae in the cortical bone of the humerus mid-shaft may suggest a large genome size and associated neotenic condition for this species, which could, in turn, be a partial explanation for the large size of H. lindae. The low metabolism of H. lindae revealed here by bone histology supports the hypothesis of an ambush predatory behaviour. Finally, the lines-of-arrested-growth pattern and late ossification of specimen ANSP 21483 suggest that H. lindae probably had a long juvenile stage before reaching sexual maturity. Although very few studies address the life-history traits of stem tetrapods, they all propose a slow limb development for the studied taxa despite different ecological conditions and presumably distinct behaviours. The bone histology of H. lindae would favour the hypothesis that a slow long-bone development could be a general character for stem tetrapods.

scholarly journals Rac signaling in osteoblastic cells is required for normal bone development but is dispensable for hematopoietic development

Blood ◽  
2012 ◽  
Vol 119 (3) ◽  
pp. 736-744 ◽  
Author(s):  
Steven W. Lane ◽  
Serena De Vita ◽  
Kylie A. Alexander ◽  
Ruchan Karaman ◽  
Michael D. Milsom ◽  
...  
Keyword(s):  
Bone Growth ◽  
Bone Development ◽  
Long Bone ◽  
Perivascular Niche ◽  
Hematopoietic Stem ◽  
Hsc Niche ◽  
Rac1 Gtpase

Abstract Hematopoietic stem cells (HSCs) interact with osteoblastic, stromal, and vascular components of the BM hematopoietic microenvironment (HM) that are required for the maintenance of long-term self-renewal in vivo. Osteoblasts have been reported to be a critical cell type making up the HSC niche in vivo. Rac1 GTPase has been implicated in adhesion, spreading, and differentiation of osteoblast cell lines and is critical for HSC engraftment and retention. Recent data suggest a differential role of GTPases in endosteal/osteoblastic versus perivascular niche function. However, whether Rac signaling pathways are also necessary in the cell-extrinsic control of HSC function within the HM has not been examined. In the present study, genetic and inducible models of Rac deletion were used to demonstrate that Rac depletion causes impaired proliferation and induction of apoptosis in the OP9 cell line and in primary BM stromal cells. Deletion of Rac proteins caused reduced trabecular and cortical long bone growth in vivo. Surprisingly, HSC function and maintenance of hematopoiesis in vivo was preserved despite these substantial cell-extrinsic changes. These data have implications for therapeutic strategies to target Rac signaling in HSC mobilization and in the treatment of leukemia and provide clarification to our evolving concepts of HSC-HM interactions.

scholarly journals Wnt signaling in chondroprogenitors during long bone development and growth

Bone ◽  
2020 ◽  
Vol 137 ◽  
pp. 115368
Author(s):  
Takeshi Oichi ◽  
Satoru Otsuru ◽  
Yu Usami ◽  
Motomi Enomoto-Iwamoto ◽  
Masahiro Iwamoto
Keyword(s):  
Wnt Signaling ◽  
Bone Development ◽  
Long Bone

The role of Has2 on long bone development

2008 ◽  
Vol 27 ◽  
pp. 22-23
Author(s):  
Peter J. Roughley ◽  
Judy Grover ◽  
Eunice R. Lee ◽  
Yu Yamaguchi
Keyword(s):  
Bone Development ◽  
Long Bone

scholarly journals Effects of Incubator Temperature and Oxygen Concentration During the Plateau Stage of Oxygen Consumption on Turkey Embryo Long Bone Development

2008 ◽  
Vol 87 (8) ◽  
pp. 1484-1492 ◽  
Author(s):  
E.O. Oviedo-Rondón ◽  
J. Small ◽  
M.J. Wineland ◽  
V.L. Christensen ◽  
J.L. Grimes ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Concentration ◽  
Bone Development ◽  
Long Bone

scholarly journals The mineral dissolution function of osteoclasts is dispensable for hypertrophic cartilage degradation during long bone development and growth

2014 ◽  
Vol 393 (1) ◽  
pp. 57-70 ◽  
Author(s):  
Heiani Touaitahuata ◽  
Gaelle Cres ◽  
Sylvain de Rossi ◽  
Virginie Vives ◽  
Anne Blangy
Keyword(s):  
Bone Development ◽  
Cartilage Degradation ◽  
Mineral Dissolution ◽  
Long Bone ◽  
Hypertrophic Cartilage

Retinoids and Indian Hedgehog Orchestrate Long Bone Development

The Skeleton ◽  
2004 ◽  
pp. 159-170
Author(s):  
Maurizio Pacifici ◽  
Chiara Gentili ◽  
Eleanor Golden ◽  
Eiki Koyama
Keyword(s):  
Bone Development ◽  
Long Bone ◽  
Indian Hedgehog

Matrix metalloproteinase-2 and -9 expression increases in Mycoplasma-infected airways but is not required for microvascular remodeling

2004 ◽  
Vol 287 (2) ◽  
pp. L307-L317 ◽  
Author(s):  
Peter Baluk ◽  
Wilfred W. Raymond ◽  
Erin Ator ◽  
Lisa M. Coussens ◽  
Donald M. McDonald ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Airway Inflammation ◽  
Bone Development ◽  
Large Population ◽  
Matrix Metalloproteinase 2 ◽  
Long Bone ◽  
Wild Type ◽  
Lung Weight ◽  
Microvascular Changes ◽  
Microvascular Remodeling

Murine Mycoplasma pulmonis infection induces chronic lung and airway inflammation accompanied by profound and persistent microvascular remodeling in tracheobronchial mucosa. Because matrix metalloproteinase (MMP)-2 and -9 are important for angiogenesis associated with placental and long bone development and skin cancer, we hypothesized that they contribute to microvascular remodeling in airways infected with M. pulmonis. To test this hypothesis, we compared microvascular changes in airways after M. pulmonis infection of wild-type FVB/N mice with those of MMP-9−/− and MMP-2−/−/MMP-9−/− double-null mice and mice treated with the broad-spectrum MMP inhibitor AG3340 (Prinomastat). Using zymography and immunohistochemistry, we find that MMP-2 and MMP-9 rise strikingly in lungs and airways of infected wild-type FVB/N and C57BL/6 mice, with no zymographic activity or immunoreactivity in MMP-2−/−/MMP-9−/− animals. However, microvascular remodeling as assessed by Lycopersicon esculentum lectin staining of whole-mounted tracheae is as severe in infected MMP-9−/−, MMP-2−/−/MMP-9−/− and AG3340-treated mice as in wild-type mice. Furthermore, all groups of infected mice develop similar inflammatory infiltrates and exhibit similar overall disease severity as indicated by decrease in body weight and increase in lung weight. Uninfected wild-type tracheae show negligible MMP-2 immunoreactivity, with scant MMP-9 immunoreactivity in and around growing cartilage. By contrast, MMP-2 appears in epithelial cells of infected, wild-type tracheae, and MMP-9 localizes to a large population of infiltrating leukocytes. We conclude that despite major increases in expression, MMP-2 and MMP-9 are not essential for microvascular remodeling in M. pulmonis-induced chronic airway inflammation.

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