scholarly journals Choline peak

2014 ◽  
Author(s):  
Andrew Soliman ◽  
Frank Gaillard
Keyword(s):  
Choline Peak

scholarly journals Resveratrol Is Active against Leishmania amazonensis:In VitroEffect of Its Association with Amphotericin B

2014 ◽  
Vol 58 (10) ◽  
pp. 6197-6208 ◽  
Author(s):  
Christian Ferreira ◽  
Deivid Costa Soares ◽  
Michelle Tanny Cunha do Nascimento ◽  
Lucia Helena Pinto-da-Silva ◽  
Carolina Galvão Sarzedas ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Red Wine ◽  
Biological Activities ◽  
Interleukin 4 ◽  
Spectroscopy Analysis ◽  
Mitochondrial Potential ◽  
Content Type ◽  
The Mean ◽  
Choline Peak

ABSTRACTResveratrol is a polyphenol found in black grapes and red wine and has many biological activities. In this study, we evaluated the effect of resveratrol alone and in association with amphotericin B (AMB) againstLeishmania amazonensis. Our results demonstrate that resveratrol possesses both antipromastigote and antiamastigote effects, with 50% inhibitory concentrations (IC50s) of 27 and 42 μM, respectively. The association of resveratrol with AMB showed synergy forL. amazonensisamastigotes, as demonstrated by the mean sums of fractional inhibitory index concentration (mean ΣFIC) of 0.483, although for promastigotes, this association was indifferent. Treatment with resveratrol increased the percentage of promastigotes in the sub-G0/G1phase of the cell cycle, reduced the mitochondrial potential, and showed an elevated choline peak and CH2-to-CH3ratio in the nuclear magnetic resonance (NMR) spectroscopy analysis; all these features indicate parasite death. Resveratrol also decreased the activity of the enzyme arginase in uninfected and infected macrophages with and without stimulation with interleukin-4 (IL-4), also implicating arginase inhibition in parasite death. The anti-Leishmaniaeffect of resveratrol and its potential synergistic association with AMB indicate that these compounds should be subjected to further studies of drug association therapyin vivo.

In vivo proton magnetic resonance spectroscopic (PMRS) evaluation: Emerging tool to solve the diagnostic dilemma in soft tissue sarcoma management

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 10020-10020
Author(s):  
M. Kar ◽  
S. S. Deo ◽  
V. Kumar ◽  
U. Sharma ◽  
N. R. Jagannathan ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Predictive Value ◽  
Proton Magnetic Resonance ◽  
Soft Tissue Sarcomas ◽  
Soft Tissue Tumour ◽  
Malignant Soft Tissue ◽  
Choline Peak

10020 Objective: Presently grade is the most important factor used to decide the soft tissue sarcoma management. But it is always very difficult to assign a pathologic grade because of discordance rate is 30–40%, even among experienced sarcoma oncopathologists. Again histopathology has the disadvantages of being invasive, subjective and insensitive to tumour heterogeneity. This emphasizes the need for an objective and accurate assessment of histologic type and grade of sarcoma. The aim of this study is to evaluate the correlation of in vivo proton magnetic resonance spectroscopic findings with histopathological features and validate its diagnostic efficacy in management of different types of soft tissue sarcomas. Methods: PMRS Study was performed at 1.5Tesla MRI machine with a surface coil appropriate for the location of the lesions in 25 patients. Single-voxel (SVS) study has been done in 10 cases and chemical shift imaging (CSI) study characterised the heterogeneity of the tumor in 15 cases by using point - resolved spectroscopic sequence (PRESS) with echo time TR=2,000 / TE = 30, 135 & 270 msec. The choline peak, identified at 3.2 ppm in spectra was considered as significant which shows the activity of the tumour. MRS results and histopathologic findings were correlated and P < 0.001, considered being significant. Results: Choline peak was found in 17 out of 17 patients with malignant soft tissue tumours where as three patient with benign and five treated malignant soft tissue tumour patients with no residual disease did not show any choline. In vivo spectroscopy here shows sensitivity, specificity, positive predictive value, negative predictive value and accuracy of 100% each. Conclusion: Choline peak in MRS study can predict the grade, margin status (especially CSI) and tumour activity in recurrent and / or residual soft tissue sarcoma. Most important findings here was that without using any contrast medium in study the choline peak in CSI spectroscopic evaluation could show the tumor activity at the margin of the tumor which is very important point for evaluation of residual or recurrent or completion of the surgical excision of the tumor A major study should be done to validate its efficacy for routine use in oncology. [Table: see text]

The Value of Monomer Hydrogen Proton Magnetic Resonance Spectra in the Diagnosis of Liver Benign and Malignant Lesions

2020 ◽  
Vol 10 (6) ◽  
pp. 884-888
Author(s):  
Yongsheng Li ◽  
Yuguang Zhang ◽  
Xia Zhang
Keyword(s):  
Magnetic Resonance ◽  
Liver Diseases ◽  
The Body ◽  
Resonance Spectroscopy ◽  
Liver Lesions ◽  
Software Analysis ◽  
Lipid Ratio ◽  
Malignant Liver ◽  
Malignant Liver Lesions ◽  
Choline Peak

The early diagnosis and treatment of liver disease are very important in clinics. Magnetic resonance spectroscopy (MRS) provides a non-invasive 'window' on the biochemical processes in the body. This study deployed research based on application of monomer element 1HMRS in the diagnosis of benign or malignant liver lesions. Patients with different liver diseases were recruited along with healthy controls. GE Signa Twinspeed 1.5T MRI was performed on livers along with monomeric hydrogen MRS, followed by SAGE software analysis. In healthy patient's livers, both choline and lipid peaks were observed under MRS, with occasionally glutamine and glutamic acid complex (Glx) peak and glucogen and glucose complex (Glu) peak. Malignant liver lesions had significantly elevated choline peak and choline/lipid ratio compared to control or benign lesions (P < 0.05). The accuracy, sensitivity and specificity of MRI against malignant tumors were 89.4%, 86.2% and 91.1%, respectively. These figures for choline/lipid and MRI plus choline/lipid were 87.2%, 92.3% and 90.1%, and 94.1%, 89.1% and 90.4%, respectively. Our study shows that monomeric hydrogen proton MRS might be used as the diagnostic evidences for malignant liver diseases, especially for choline peak level and choline/lipid ratio. However, large cohort multi-center study is required to confirm the findings in the future.

scholarly journals Proton MR spectroscopy in characterization of focal bone lesions of peripheral skeleton

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Meenu Amar ◽  
Rohini Gupta Ghasi ◽  
L. G. Krishna ◽  
Geetika Khanna
Keyword(s):  
Mr Spectroscopy ◽  
Bone Lesions ◽  
Water Suppression ◽  
Low Grade ◽  
Benign Lesions ◽  
Giant Cell Tumors ◽  
Proton Mr Spectroscopy ◽  
Malignant Lesions ◽  
Peripheral Skeleton ◽  
Choline Peak

Abstract Background The aim of our study was to determine the value of single-voxel proton MR spectroscopy (1HMRS) in distinguishing benign from malignant focal bone lesions in the peripheral skeleton. MRI and 1HMRS was performed in 50 focal lesions (> 1 cm size) detected on radiographs of peripheral skeleton.1HMRS was performed at 1.5 T with TE of 144 ms with automatic shimming and water suppression. Qualitative analysis for a discrete choline peak at 3.2 ppm was done. Significance of the presence of choline peak on 1HMRS in distinguishing benign from malignant lesions was calculated using histopathology as a gold standard. Chi-square test was used and p value < 0.05 was considered significant. Results Forty-one benign and 9 malignant lesions were confirmed by histopathological results. Amongst malignant lesions, choline peak was positive in all but 1 case of low-grade lymphoma. MR spectra of 11 benign lesions showed the presence of choline peak. All 7 benign giant cell tumors (GCT) were positive for choline peak. The sensitivity, specificity, PPV, NPV of proton MR spectroscopy in differentiating benign from malignant lesions were 87.5%,71%,38.8%, and 96.4% respectively. p value was significant (< 0.05). Conclusion 1HMRS in focal bone lesions can help in the differentiation of malignant from benign musculoskeletal tumors. Although some benign lesions may show false-positive result, absence of choline peak is a reliable reassurance against malignancy. GCT is an exception amongst benign bone tumors as it consistently shows the presence of choline peak on 1HMRS.

scholarly journals Advanced MRI Techniques in the Evaluation of Complex Cystic Breast Lesions

2016 ◽  
Vol 10 ◽  
pp. BCBCR.S38535
Author(s):  
Manju Bala Popli ◽  
Pranav Gupta ◽  
Devraj Arse ◽  
Pawan Kumar ◽  
Prabhjot Kaur
Keyword(s):  
Diffusion Coefficient ◽  
Apparent Diffusion Coefficient ◽  
Mr Spectroscopy ◽  
Kinetic Curve ◽  
Breast Lesions ◽  
Cystic Lesions ◽  
Type Iii ◽  
Diffusion Weighted ◽  
Apparent Diffusion ◽  
Choline Peak

Objective The purpose of this research work was to evaluate complex cystic breast lesions by advanced MRI techniques and correlating imaging with histologic findings. Methods and Materials In a cross-sectional design from September 2013 to August 2015, 50 patients having sonographically detected complex cystic lesions of the breast were included in the study. Morphological characteristics were assessed. Dynamic contrast-enhanced MRI along with diffusion-weighted imaging and MR spectroscopy were used to further classify lesions into benign and malignant categories. All the findings were correlated with histopathology. Results Of the 50 complex cystic lesions, 32 proved to be benign and 18 were malignant on histopathology. MRI features of heterogeneous enhancement on CE-MRI (13/18), Type III kinetic curve (13/18), reduced apparent diffusion coefficient (18/18), and tall choline peak (17/18) were strong predictors of malignancy. Thirteen of the 18 lesions showed a combination of Type III curve, reduced apparent diffusion coefficient value, and tall choline peak. Conclusions Advanced MRI techniques like dynamic imaging, diffusion-weighted sequences, and MR spectroscopy provide a high level of diagnostic confidence in the characterization of complex cystic breast lesion, thus allowing early diagnosis and significantly reducing patient morbidity and mortality. From our study, lesions showing heterogeneous contrast enhancement, Type III kinetic curve, diffusion restriction, and tall choline peak were significantly associated with malignant complex cystic lesions of the breast.

scholarly journals Proton (1H) MR Spectroscopy of the Breast at 3.0T: Detectability of the Choline Peak of Breast Cancer in Comparison with a 1.5T Imager

2010 ◽  
Vol 22 (4) ◽  
pp. 221-227
Author(s):  
Makoto SAIKI ◽  
Masanori HIROSE ◽  
Jumpei SUYAMA ◽  
Yoshimitsu OHGIYA ◽  
Takehiko GOKAN
Keyword(s):  
Breast Cancer ◽  
Mr Spectroscopy ◽  
1H Mr Spectroscopy ◽  
Choline Peak

scholarly journals The Effect of Paramagnetic Contrast in Choline Peak in Patients with Glioblastoma Multiforme Might Not Be Significant

2012 ◽  
Vol 34 (1) ◽  
pp. 80-84 ◽  
Author(s):  
E.C. Lima ◽  
M.C.G. Otaduy ◽  
M. Tsunemi ◽  
R. Pincerato ◽  
E.F. Cardoso ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Choline Peak

The key role of correlation between MR spectroscopy choline peak and calcium sensing receptor for breast cancer: Diagnosis by a 3T MR scanner.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e22132-e22132
Author(s):  
Gabriella Baio ◽  
Francesca Valdora ◽  
Daniele Pace ◽  
Sandra Salvi ◽  
Nicolo Villosio ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mr Spectroscopy ◽  
Breast Lesions ◽  
Breast Cancers ◽  
Calcium Sensing Receptor ◽  
Calcium Sensing ◽  
Malignant Lesions ◽  
High Level ◽  
3T Mr ◽  
Choline Peak

e22132 Background: The Ca2+-sensing receptor (CaSR) regulates the Ca2+ signaling and its expression is present in both normal and malignant breast tissues. Recently, we demonstrated in a pre-clinical setting the CaSR expression in breast cancer mouse model by manganese enhanced MRI (Baio G et al. Eur Rad 2011). Alteration in choline phospholipid metabolism as detected by MRI, is a common feature of breast and many other cancer cells or tumors. Since CaSR-ChoK signaling plays an important role in breast cancer, we compared the MRS (MR spectroscopy) choline peak by a 3T MR scanner between calcium sensing receptor positive and negative breast cancers. Methods: Breast MRI of 16 consecutive patients with breast lesions were performed by a 3T MR and MRS findings were defined as positive by the visual inspection for the presence or the absence of choline peak. MRS imaging were compared with histologic findings and the pattern of CaSR by immunohistochemistry. The immunohistochemical results were qualitatively classified according to intensity and pattern of the staining, using a 6-point scale to score the intensity of the CaSR expression (0, absent; 5, intense, widespread expression). Results: A choline peak was present in 10 of 15 malignant lesions and the CaSR expression was between 3 to 5 score at immunohistochemestry analysis (p<.0006). In 4 of 15 malignant lesions the choline peak was absent or not consistent at the visual analysis with a CaSR patterns of 1-2. In one benign lesion (papillary hyperplasia), the choline peak was consistent and the CaSR pattern was with score 3. Conclusions: In this study we evaluated if MRS presence or absence of choline peak well correlated with CaSR positive and negative breast cancers. We observed a choline peak with an high level expression of CaSR (score 3-5) in 67% of the breast lesions, while in 27% of the lesions the choline peak was absent with a low CaSR expression (score 1-2). These preliminary results support the hypothesis that CaSR represents an important role in the production of choline in breast cancer, determining an increase of his production when CaSR is expressed at high level and implicating and absence of choline peak when CaSR is not expressed.

Sign in / Sign up

Export Citation Format

Share Document