The Clinical Utility of Circulating Tumor Cells in Breast Cancer Patients: Detection by a Quantitative Assay of h-MAM Gene Expression

2014 ◽  
Vol 29 (3) ◽  
pp. 268-278 ◽  
Author(s):  
Min Cheng ◽  
Yongyan Chen ◽  
Dehong Zou ◽  
Guodong Shen ◽  
Geng Bian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Detection Rate ◽  
Benign Breast Disease ◽  
Breast Disease ◽  
Expression Level ◽  
Breast Cancer Patients ◽  
Healthy Individuals ◽  
Epithelial Cancers ◽  
Erbb2 Mrna

The aim of this study was to evaluate tumor markers of molecular abnormalities that display tissue specificity, as to detect circulating tumor cells (CTCs) in breast cancer patients. Quantitative real-time RT-PCR was used to determine h-MAM, BCSG1, CK19, and c-erbB2 mRNA levels in peripheral blood (PB) of breast cancer patients. Results were compared with other epithelial cancers (lung or esophagus cancer), benign breast disease, and healthy individuals. We found that h-MAM mRNA was only detectable in the PB of patients with breast cancer (49 of 65, 75.4%), but not in patients with other epithelial cancers, benign breast disease, or healthy individuals. No significant differences in the expression level and positive detection rate of BCSG1, CK19, and c-erbB2 mRNA were observed between breast cancer and other epithelial cancers. Furthermore, the expression level and positive detection rate of h-MAM mRNA in PB were significantly correlated to the breast cancer pathologic stage (p=0.012 and p=0.015, respectively). Chemotherapy, radiotherapy, or total tumor resection (after 7 days of treatment) resulted in a significant decrease in the expression level of h-MAM mRNA in PB compared to the levels prior to the treatment (p<0.001). Importantly, an increase in h-MAM mRNA expression was detected in patients immediately after surgery, as well as 3 days post-surgery. These results indicate that the quantitative analysis of h-MAM mRNA is a useful tool for detecting CTCs in breast cancer patients, and can have a potential diagnostic utility in early micrometastasis, clinical evaluation of cancer treatment efficacy, and post-treatment monitoring of breast cancer patients.

scholarly journals Serum levels of oxidative stress marker malondialdehyde in breast cancer patients in relation to pathohistological factors, estrogen receptors, menopausal status, and age

2018 ◽  
Vol 8 (3) ◽  
pp. 154-161
Author(s):  
Jasmina Gubaljevic ◽  
Nahida Srabović ◽  
Adlija Jevrić-Čaušević ◽  
Adaleta Softić ◽  
Adi Rifatbegović ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Benign Breast Disease ◽  
Menopausal Status ◽  
Serum Levels ◽  
Breast Disease ◽  
Breast Cancer Patients ◽  
Node Metastases

Introduction: The aim of this study was to determine the serum levels of malondialdehyde (MDA) in patients with invasive breast cancer in relation to its serum levels in patients with benign breast disease, and to investigate correlation between MDA serum levels with pathohistological prognostic factors (tumor size, lymph node involvement, and histologic grade [HG]), estrogen receptor (ER) status, and with breast cancer patient’s age and menopausal status. Methods: A total of 43 with well-documented invasive breast cancer were included in this study: 27 with positive axillary’s lymph nodes, and 16 with negative axillary’s lymph nodes, and 39 patients with findings of benign breast diseases. MDA determination in serum of breast cancer and benign breast disease patients was performed by the fluorimetric method, immunohistochemical staining was performed for ER, and routine pathohistological examination was conducted for pathohistological factors. Results: MDA serum levels in breast cancer patients were significantly higher than MDA serum levels in benign breast disease patients (p = 0.042). No statistically significant difference between MDA serum levels in breast cancer patients with and without lymph node metastases was found (p = 0.238). No statistically significant correlations between MDA serum levels and tumor size (p = 0.256), HG (p = 0.124), or number of positive lymph nodes (0.113) were found. A statistically significant correlation between serum MDA levels and ages of breast cancer patients with lymph node metastases was found (p = 0.006). Conclusion: Obtained results support the importance of MDA in the carcinogenesis of breast cancer. According to our findings, serum level of MDA could not be a useful prognostic factor in breast cancer.

scholarly journals Serum Vitamin Levels and Their Relationships with Other Biomarkers in Korean Breast Cancer Patients

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2831
Author(s):  
Jee Ah Kim ◽  
Rihwa Choi ◽  
Hojeong Won ◽  
Seonwoo Kim ◽  
Hee Jun Choi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
Benign Breast Disease ◽  
Serum Vitamin ◽  
Breast Disease ◽  
Healthy Controls ◽  
Breast Cancer Patients ◽  
Tumor Stages

Numerous studies have shown that vitamins reduce the risk of cancers, but the relationship between serum vitamin levels and breast cancer is still controversial. In this study, we evaluated serum levels of vitamins in Korean patients with benign breast disease or breast cancer and investigated their associations with clinical and laboratory parameters. Concentrations of vitamin A, D, and E, together with homocysteine and methylmalonic acid as biomarkers of vitamin B12 deficiency, were measured by high-performance liquid chromatography (HPLC) or liquid chromatography with tandem mass spectrometry (LC-MS/MS) in the serum of 104 breast cancer patients, 62 benign breast disease patients, and 75 healthy Korean females. We further assessed possible associations between vitamin levels and breast cancer subtypes, the presence of lymph node metastasis, and tumor stages. Serum concentrations of vitamins A and E were significantly lower in breast cancer patients and in benign breast disease patients than in healthy controls. Severe vitamin D deficiency was more prevalent in breast cancer patients than in healthy controls. Vitamin D level was significantly lower in breast cancer patients with estrogen receptor-negative or triple-negative subtypes than in those with other subtypes. Further research with a larger study population is required to elucidate the role of vitamins in breast cancer.

scholarly journals Preoperative Peripheral Blood Count in Breast Carcinoma: Predictor of Prognosis or a Routine Test

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Amrit Pal Singh Rana ◽  
Manjit Kaur ◽  
B. Zonunsanga ◽  
Arun Puri ◽  
Amarjit Singh Kuka
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Blood Count ◽  
Benign Breast Disease ◽  
Case Control Study ◽  
Breast Disease ◽  
Case Control ◽  
Breast Cancer Patients ◽  
Peripheral Blood Count ◽  
Control Study

Background.Peripheral blood count is the first investigation to be done in every patient before surgery. As strong relationship exists between cancer and immune response of the body, clinical stage at presentation and altered hematological parameters can influence the progression of cancer and vice versa.Settings and Design.It is a case control study of total 50 cases (35 cases of carcinoma breast and 15 cases of benign breast disease).Methods.A case control study was carried out; 35 cases of breast cancer patients were taken prior to surgery and chemotherapy with 15 cases of benign breast disease as control. Clinical staging according to the tumor, node, and metastasis classification (TNMc) was done and was correlated with complete blood count (CBC).Results.All the cancer patients were females with overall mean age of47.96±13.84years. Amongst all altered blood parameters, correlation of absolute lymphocytic count (pvalue 0.001) with TNMc staging was found significant. Particularly, decrease in absolute leucocytic count was observed with increase in stage of breast carcinoma.Conclusions. The stage-specific mean values of absolute lymphocytic counts of preoperative breast cancer patients can be used as an economical tool to know the evolution of disease.

Retinoids, Carotenoids, and Tocopherols in Breast Adipose Tissue and Serum of Benign Breast Disease and Breast Cancer Patients

2012 ◽  
Vol 64 (7) ◽  
pp. 956-963 ◽  
Author(s):  
Eugene Shim ◽  
Kyung-Jin Yeum ◽  
Guangwen Tang ◽  
Sei Hyun Ahn ◽  
Jinah Hwang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Adipose Tissue ◽  
Cancer Patients ◽  
Benign Breast Disease ◽  
Breast Disease ◽  
Breast Cancer Patients ◽  
Breast Adipose Tissue ◽  
Breast Adipose

scholarly journals Does HOTAIR expression level in the peripheral blood have veritably predictive/prognostic impact on breast cancer patients?

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Vahid Ezzatizadeh ◽  
Hossein Mozdarani
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Expression Level ◽  
Prognostic Impact ◽  
Breast Cancer Patients ◽  
Blood Biomarker ◽  
Non Coding Rna ◽  
Before And After ◽  
Significant Expression

AbstractAs a peripheral blood biomarker, the crucial role of long non-coding RNA (lncRNA) HOTAIR has recently been suggested in many types of disorder. Among these reports, few investigations have indicated overexpression of HOTAIR transcript in the breast cancer patients’ peripheral blood. In this regard, we studied the potential impact of radiotherapy on the peripheral blood HOTAIR expression of different breast cancer patients. Curiously, no significant expression level of HOTAIR was determined in the breast cancer patients’ peripheral blood, before and after radiotherapy (10 Gy exposure). Deliberating these investigations raised some debates on the specificity of the utilized methods, the corresponding obtained findings and impact of HOTAIR expression on breast cancer predication, as a potential peripheral blood biomarker, which is discussed in this article.

scholarly journals Altered Impedance of Ear Acupuncture Point MT2 in Breast Cancer Patients: A Preliminary Observation

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Yine Hu ◽  
Huayuan Yang ◽  
Pin Wang ◽  
Tangyi Liu ◽  
Wenchao Tang
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Acupuncture Point ◽  
Preliminary Observation ◽  
Ear Acupuncture ◽  
Skin Impedance ◽  
Acupuncture Points ◽  
Breast Cancer Patients ◽  
Healthy Individuals ◽  
Healthy Humans

Skin impedance at acupuncture points (APs) has been used as a diagnostic aid for more than 50 years. In this study, we have a diagnostic tool (JXT-2008) to measure the skin impedance of ear APs of 30 breast cancer patients and the corresponding skin impedance of ear APs of 30 healthy humans, and then we compared these changes in ear AP impedance in breast cancer patients and healthy individuals.

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