Alendronate Stimulates Osteoprotegerin Expression in Fibroblasts from Periprosthetic Membrane

2015 ◽  
Vol 25 (6) ◽  
pp. 581-584 ◽  
Author(s):  
Yebin Qian ◽  
Xian-long Zhang ◽  
Yao Jiang ◽  
Bing-Fang Zeng ◽  
Qi Wang ◽  
...  
Keyword(s):  
Periprosthetic Membrane

Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane

2013 ◽  
Vol 54 (6) ◽  
pp. 361-366 ◽  
Author(s):  
Yebin Qian ◽  
Xian-long Zhang ◽  
Bing-Fang Zeng ◽  
Yao Jiang ◽  
Hao Shen ◽  
...  
Keyword(s):  
Substance P ◽  
Rankl Expression ◽  
Periprosthetic Membrane

scholarly journals Naringin Increases Osteoprotegerin Expression in Fibroblasts From Periprosthetic Membrane by the Wnt/β-catenin Signaling Pathway

2020 ◽  
Author(s):  
Chao Yang ◽  
Wei Liu ◽  
Xianlong Zhang ◽  
Bingfang Zeng ◽  
Yebin Qian
Keyword(s):  
Gene Expression ◽  
Cyclin D1 ◽  
Aseptic Loosening ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Osteoclast Formation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Protein Levels ◽  
Periprosthetic Membrane

Abstract Background: The osteoclast bone resorption is critical in aseptic loosening after joint replacemrent. The balance between activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) is considered to play a central role in osteoclast maturation. Fibroblasts from the periprosthetic membrane express RANKL and promote osteoclast formation. Studies have demonstrated that naringin inhibited osteoclastogenesis and wear particles-induced osteolysis. In this study, the naringin-induced OPG/RANKL effects and its underlying mechanism were studied in fibroblasts from periprosthetic membrane. Methods: Fibroblasts were isolated from the periprosthetic membrane during hip arthroplasty for revision due to aseptic loosening. Fibroblasts were cultured and treated with or without naringin and DKK-1 (the classical inhibitor of Wnt/β-catenin signaling pathway). OPG and RANKL mRNA and protein levels, gene expression of β-catenin and cyclin D1, which participate in the Wnt signaling pathway, were examined by real-time polymerase chain reaction and enzyme- linked immunosorbent assay. Results: The mRNA and protein levels of OPG were enhanced by naringin in a dose dependent manner compared to that of the non-treated control. In contrast, naringin did not affect the expression of RANKL. Importantly, DKK-1 attenuated OPG expression in fibroblasts under naringin treatment. Moreover, naringin stimulated the gene expression of β-catenin and cyclin D1 in fibroblasts and the effect could be inhibited by DKK-1.Conclusion: The results indicated that naringin enhanced OPG expression through Wnt/β-catenin signaling pathway in fibroblasts from periprosthetic membrane, which may be useful to inhibit periprosthetic osteolysis during aseptic loosening after total joint arthroplasty.

Hip- and knee-endoprosthesis loosening: proposal for a histomorphological classification of the periprosthetic membrane

2004 ◽  
Vol 200 (4) ◽  
pp. 267
Author(s):  
L. Morawietz ◽  
R.A. Classen ◽  
T. Gehrke ◽  
M. Schwabe ◽  
J Kriegsmann ◽  
...  
Keyword(s):  
Periprosthetic Membrane ◽  
Endoprosthesis Loosening ◽  
Knee Endoprosthesis

Immunohistochemical characterization of a new type of periprosthetic membrane (indifferent type)

2004 ◽  
Vol 200 (4) ◽  
pp. 331
Author(s):  
M. Schwabe ◽  
R.A. Classen ◽  
L. Morawietz ◽  
J.H. Schroeder ◽  
V. Krenn
Keyword(s):  
Periprosthetic Membrane ◽  
New Type

Usefulness of Culturing the Periprosthetic Membrane or Neosynovium for the Diagnosis of Infection During Hip and Knee Revision Arthroplasty

2018 ◽  
Vol 26 (20) ◽  
pp. e442-e447 ◽  
Author(s):  
Ernesto Muñoz-Mahamud ◽  
Ignacio Molinas ◽  
Luis Lozano ◽  
Jordi Bosch ◽  
Laura Morata ◽  
...  
Keyword(s):  
Revision Arthroplasty ◽  
Periprosthetic Membrane ◽  
Knee Revision ◽  
Diagnosis Of Infection

scholarly journals Naringin increases osteoprotegerin expression in fibroblasts from periprosthetic membrane by the Wnt/β-catenin signaling pathway

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Chao Yang ◽  
Wei Liu ◽  
Xianlong Zhang ◽  
Bingfang Zeng ◽  
Yebin Qian
Keyword(s):  
Gene Expression ◽  
Cyclin D1 ◽  
Aseptic Loosening ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Osteoclast Formation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Protein Levels ◽  
Periprosthetic Membrane

Abstract Background The osteoclast bone resorption is critical in aseptic loosening after joint replacement. The balance between activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) is considered to play a central role in osteoclast maturation. Fibroblasts from the periprosthetic membrane express RANKL and promote osteoclast formation. Studies have demonstrated that naringin inhibited osteoclastogenesis and wear particle-induced osteolysis. In this study, the naringin-induced OPG/RANKL effects and its underlying mechanism were studied in fibroblasts from periprosthetic membrane. Methods Fibroblasts were isolated from the periprosthetic membrane during hip arthroplasty for revision due to aseptic loosening. Fibroblasts were cultured and treated with or without naringin and DKK-1 (the classical inhibitor of Wnt/β-catenin signaling pathway). OPG and RANKL mRNA and protein levels, gene expression of β-catenin, and cyclin D1, which participate in the Wnt signaling pathway, were examined by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results The mRNA and protein levels of OPG were enhanced by naringin in a dose-dependent manner compared to that of the non-treated control. In contrast, naringin did not affect the expression of RANKL. Importantly, DKK-1 attenuated OPG expression in fibroblasts under naringin treatment. Moreover, naringin stimulated the gene expression of β-catenin and cyclin D1 in fibroblasts, and the effect could be inhibited by DKK-1. Conclusion The results indicated that naringin enhanced OPG expression through Wnt/β-catenin signaling pathway in fibroblasts from periprosthetic membrane, which may be useful to inhibit periprosthetic osteolysis during aseptic loosening after total joint arthroplasty.

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