scholarly journals Selection of glufosinate-resistant cotton lines (Gossypium hirsutum L.) among bar transgenic lines

2020 ◽  
Vol 19 (05) ◽  
pp. 71-79
Author(s):  
Nha T. Nguyen
Keyword(s):  
Disease Resistance ◽  
Target Gene ◽  
Transgenic Cotton ◽  
Gene Copy ◽  
Selected Lines ◽  
Gossypium Hirsutum L ◽  
Transgenic Lines ◽  
Botanical Characteristics ◽  
Biological Assessments ◽  
Selection Of

Basta-herbicide was tested at a concentration of 0.6 kg ai./ha for confirming resistance of 116 bar transgenic T1 lines; many lines with tolerance were obtained. Evaluation of selected lines using PCR, the integration and expression of transgenes in genome of transgenic plants was determined by southern blot and northern blot techniques. The combination of molecular and biological assessments resulted in the selection of 5 lines, i.e., B1, B6, B9, B18, and BF17 contained 01 target-gene copy which expressed transcription activities and showed uniform growth and best tolerance to glufosinate. Two T2 transgenic cotton lines, i.e., B9 and BF17, carried one copy of the gene which transmitted to the next generation according to the Mendel's rules of inheritance. These transgenic lines were highly resistant to Basta herbicide at a concentration of 0.6 kg ai./ha and had no difference in botanical characteristics and disease resistance in comparison with original non-transgenic Coker310 cotton plant

IN VITRO SELECTION OF DISEASE RESISTANCE IN FRUIT TREES

1995 ◽  
pp. 19-26 ◽  
Author(s):  
F. Hammerschlag ◽  
D. Ritchie ◽  
D. Werner ◽  
G. Hashmil ◽  
L. Krusberg ◽  
...  
Keyword(s):  
Disease Resistance ◽  
In Vitro Selection ◽  
Fruit Trees ◽  
Selection Of

A novel T-DNA vector design for selection of transgenic lines with simple transgene integration and stable transgene expression

2005 ◽  
Vol 32 (8) ◽  
pp. 671 ◽  
Author(s):  
Song Chen ◽  
Christopher A. Helliwell ◽  
Li-Min Wu ◽  
Elizabeth S. Dennis ◽  
Narayana M. Upadhyaya ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Marker Gene ◽  
Direct Repeat ◽  
Transgene Silencing ◽  
Selective Marker ◽  
Hairpin Rna ◽  
Transgenic Lines ◽  
Flanking Sequences ◽  
Dna Vector ◽  
Selection Of

Plants transformed with Agrobacterium frequently contain T-DNA concatamers with direct-repeat (d / r) or inverted-repeat (i / r) transgene integrations, and these repetitive T-DNA insertions are often associated with transgene silencing. To facilitate the selection of transgenic lines with simple T-DNA insertions, we constructed a binary vector (pSIV) based on the principle of hairpin RNA (hpRNA)-induced gene silencing. The vector is designed so that any transformed cells that contain more than one insertion per locus should generate hpRNA against the selective marker gene, leading to its silencing. These cells should, therefore, be sensitive to the selective agent and less likely to regenerate. Results from Arabidopsis and tobacco transformation showed that pSIV gave considerably fewer transgenic lines with repetitive insertions than did a conventional T-DNA vector (pCON). Furthermore, the transgene was more stably expressed in the pSIV plants than in the pCON plants. Rescue of plant DNA flanking sequences from pSIV plants was significantly more frequent than from pCON plants, suggesting that pSIV is potentially useful for T-DNA tagging. Our results revealed a perfect correlation between the presence of tail-to-tail inverted repeats and transgene silencing, supporting the view that read-through hpRNA transcript derived from i / r T-DNA insertions is a primary inducer of transgene silencing in plants.

scholarly journals Селекция лука репчатого с устойчивостью к пероноспорозу

2019 ◽  
Author(s):  
G.F. Monakhos ◽  
S.G. Monakhos ◽  
R.R. Alizhanova
Keyword(s):  
Disease Resistance ◽  
Downy Mildew ◽  
Resistance Gene ◽  
Inbred Lines ◽  
F1 Hybrids ◽  
Downy Mildew Resistance ◽  
European Origin ◽  
Molecular Genotyping ◽  
Breeding Station ◽  
Selection Of

На Селекционной станции имени Н.Н. Тимофеева гибридизацией с донором устойчивости к пероноспорозу F1 Santero, беккроссом, инбридингом и отбором на инфекционном фоне с помощью молекулярного маркера DMR1 создано 15 линий лука репчатого гомозиготного по гену устойчивости Pd1. Оценка в однолетней культуре позволила выделить линии с массой луковиц 100120 г с высокой сохранностью, которые могут быть использованы в селекции F1 гибридов в качестве отцовских компонентов.Fifteen homozygous for Pd1 downy mildew resistance gene onion lines were developed by hybridization followed backcrossing F1 Santero of downy mildew resistant onion accession of European origin and a collection of onion inbred lines from LC Breeding station after N.N. Timofeev. The resistant to downy mildew, caused by Peronospora destructor (Berk.), plants were selected in segregated populations based on disease resistance test and molecular genotyping using DMR1marker. The lines with a mass of bulbs 100 120 g with high preservation, which can be used in the selection of F1 hybrids as paternal components.

Transgenic Rice Expressing Isoflavone Synthase Gene from Soybean Shows Resistance against Blast Fungus (Magnaporthe oryzae)

Plant Disease ◽  
2021 ◽  
Author(s):  
Suresh Pokhrel ◽  
Sathish K Ponniah ◽  
Yulin Jia ◽  
Oliver Yu ◽  
Muthusamy Manoharan
Keyword(s):  
Disease Resistance ◽  
Transgenic Rice ◽  
Magnaporthe Oryzae ◽  
Plant Disease Resistance ◽  
Rice Blast ◽  
Plant Disease ◽  
Plant Secondary Metabolites ◽  
Single Copy ◽  
Transgenic Lines ◽  
Isoflavone Synthase

The isoflavones are a group of plant secondary metabolites primarily synthesized in legumes and are known for their role in improving human health and plant disease resistance. The isoflavones, especially genistein, act as precursors for the production of phytoalexins, which may induce broad-spectrum disease resistance in plants. In the present study, we screened transgenic rice lines expressing the isoflavone synthase (GmIFS1) gene from soybean for rice blast (Magnaporthe oryzae) resistance. Two homozygous transgenic lines (I2 and I10), based on single copy gene integration, were identified. The expression of GmIFS1 in transgenic lines was confirmed by qRT-PCR. Genistein was detected in the transgenic lines using LC-MS/MS. Subsequently, the transgenic lines were evaluated against the rice blast pathogen, isolate YJ54 (race IB-54). The results indicated that more than 60% of the plants in both the lines (I2 and I10) showed resistance against the blast pathogen. The progenies of one of the resistant transgenic lines (I10) also showed more than 65% resistance against rice blast. The resistance of these transgenic lines against rice blast may be attributed to the synthesis of isoflavone (genistein) in rice.

scholarly journals WRKY Transcription Factors Shared by BTH-Induced Resistance and NPR1-Mediated Acquired Resistance Improve Broad-Spectrum Disease Resistance in Wheat

2020 ◽  
Vol 33 (3) ◽  
pp. 433-443 ◽  
Author(s):  
Huanpeng Li ◽  
Jiaojiao Wu ◽  
Xiaofeng Shang ◽  
Miaomiao Geng ◽  
Jing Gao ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Disease Resistance ◽  
Induced Resistance ◽  
Broad Spectrum ◽  
Puccinia Striiformis ◽  
Acquired Resistance ◽  
Wrky Transcription Factors ◽  
Pathogen Invasion ◽  
Pathogenesis Related ◽  
Transgenic Lines

In Arabidopsis, both pathogen invasion and benzothiadiazole (BTH) treatment activate the nonexpresser of pathogenesis-related genes 1 (NPR1)-mediated systemic acquired resistance, which provides broad-spectrum disease resistance to secondary pathogen infection. However, the BTH-induced resistance in Triticeae crops of wheat and barley seems to be accomplished through an NPR1-independent pathway. In the current investigation, we applied transcriptome analysis on barley transgenic lines overexpressing wheat wNPR1 (wNPR1-OE) and knocking down barley HvNPR1 (HvNPR1-Kd) to reveal the role of NPR1 during the BTH-induced resistance. Most of the previously designated barley chemical-induced (BCI) genes were upregulated in an NPR1-independent manner, whereas the expression levels of several pathogenesis-related (PR) genes were elevated upon BTH treatment only in wNPR1-OE. Two barley WRKY transcription factors, HvWRKY6 and HvWRKY70, were predicted and further validated as key regulators shared by the BTH-induced resistance and the NPR1-mediated acquired resistance. Wheat transgenic lines overexpressing HvWRKY6 and HvWRKY70 showed different degrees of enhanced resistance to Puccinia striiformis f. sp. tritici pathotype CYR32 and Blumeria graminis f. sp. tritici pathotype E20. In conclusion, the transcriptional changes of BTH-induced resistance in barley were initially profiled, and the identified key regulators would be valuable resources for the genetic improvement of broad-spectrum disease resistance in wheat. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Copper affects the binding of HIF-1α to the critical motifs of its target genes

Metallomics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 429-438 ◽  
Author(s):  
Zhijuan Wu ◽  
Wenjing Zhang ◽  
Y. James Kang
Keyword(s):  
Dna Binding ◽  
Target Gene ◽  
Target Genes ◽  
Gene Selection ◽  
Hypoxic Conditions ◽  
Selection Of

Copper regulates the target gene selection of HIF-1α under hypoxic conditions by affecting HIF-1α-DNA binding patterns across the genome.

Coat protein 3 of Rice tungro spherical virus is the key target gene for development of RNAi mediated tungro disease resistance in rice

Agri Gene ◽  
2019 ◽  
Vol 12 ◽  
pp. 100084 ◽  
Author(s):  
P. Malathi ◽  
Shaik Abdul Muzammil ◽  
D. Krishnaveni ◽  
S.M. Balachandran ◽  
Satendra K. Mangrauthia
Keyword(s):  
Disease Resistance ◽  
Coat Protein ◽  
Target Gene ◽  
Rice Tungro Spherical Virus

scholarly journals Population structure in artificial selection: studies with Drosophila melanogaster

1974 ◽  
Vol 24 (2) ◽  
pp. 113-126 ◽  
Author(s):  
F. E. Madalena ◽  
Alan Robertson
Keyword(s):  
Drosophila Melanogaster ◽  
Selection Procedure ◽  
Pleiotropic Effect ◽  
Single Cycle ◽  
Selected Lines ◽  
Cycle Structure ◽  
High Frequencies ◽  
Sixth Generation ◽  
Final Response ◽  
Selection Of

SUMMARYThe effect of sub-dividing a population during selection was studied by selecting downwards for sternopleural bristles in Drosophila melanogaster. The four structures used all involved the selection of 40 individuals out of 200 measured in each sex. The alternatives were:(i) one large line with the same selection procedure in all generations,(ii) eight small lines with the same procedure in all generations,(iii) the ‘single cycle’ structure in which large lines derived from crosses between selected small lines at either the sixth or twelth generation, and,(iv) the ‘repeated cycle’ structure in which the best five small lines were mixed at the sixth generation of selection to produce a new set of small lines on which the procedure was repeated for a total of three cycles.Of the first two methods, the large line had a higher final response than did any of the small lines. The latter, selected with an intensity of 5 out of 25 in each sex, had lower average final response than had a similar group of lines selected from the same base population with the lower intensity of 10/25. The results showed no clear effect of the sub-dicision of the population, followed by selection between lines, though they were in general agreement with theortical expectations. Several second chromosome recessive lethals were found at high frequencies in different selected lines. One of these, found in eight of the lines, had an effect on bristles in the heterzygote of about 1·5 phenotypic standard deviations. The evidence strongly suggests a pleiotropic effect on bristle score.

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