Q2-test: a new fast and accurate seed quality evaluation test based on automatic single seed respiration measurements; examples from sugar beett seed research

2005 ◽  
Author(s):  
Bert Van Dujin ◽  
Mieke Van Zeijl ◽  
Johan Van Asbrouck
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Single Seed ◽  
Evaluation Test ◽  
Seed Respiration

Single-seed respiration: a new method to assess seed quality

2013 ◽  
Vol 41 (3) ◽  
pp. 420-438 ◽  
Author(s):  
K.J. Bradford ◽  
P. Bello ◽  
J.-C. Fu ◽  
M. Barros
Keyword(s):  
Seed Quality ◽  
New Method ◽  
Single Seed ◽  
Seed Respiration

scholarly journals Seed Quality Evaluation by Tetrazolium Staining of <i>Parkia multijuga</i> Benth

2018 ◽  
Vol 09 (05) ◽  
pp. 577-586 ◽  
Author(s):  
Marcelle Auday Costa ◽  
Elizabeth Santos Cordeiro Shimizu ◽  
Noemi Vianna Martins Leão ◽  
Hugo Alves Pinheiro
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Tetrazolium Staining

scholarly journals Seed Quality Evaluation of Senegalia polyphylla (DC.) Britton & Rose

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Patricia Gibbert ◽  
Michele Fernanda Bortolini ◽  
Daiana Karoline Kaiser ◽  
Evelin Maria Muller ◽  
Francine Gusatto
Keyword(s):  
Quality Evaluation ◽  
Seed Quality

QUALITY CHANGES OCCURRING IN SMALL LOTS OF DRY AND MOIST RAPESEED DURING STORAGE

1980 ◽  
Vol 60 (3) ◽  
pp. 831-839 ◽  
Author(s):  
J. T. MILLS
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Quality Changes ◽  
Penicillium Verrucosum ◽  
Brassica Napus L ◽  
Post Harvest ◽  
High Germination ◽  
Wallemia Sebi ◽  
Aspergillus Candidus ◽  
Seed Respiration

Small, 25-kg quantities of moist and dry Brassica napus L. ’Tower’ rapeseed were stored in drums located outdoors for 65 wk. Quality changes occurring in the moist (10.9–15.0% moisture content) rapeseed, which was deliberately allowed to spoil, were compared to those in dry (4.3–4.9% moisture content) seed. Moist seeds in the first 7 wk had high respiration and declined in quality during the 65 wk of storage as indicated by decreased seed germination, increased leakage of seed electrolytes, and increased free fatty acid (FAV) levels. Levels of post-harvest molds, including Aspergillus candidus, A. glaucus group species, A. versicolor, Penicillium verrucosum var. cyclopium and Wallemia sebi, also increased. Mycelia of P. verrucosum var. cyclopium caused seeds of 12.9–17.8% moisture content to become cemented together at the bottom of some drums necessitating removal of samples by chipping at 65 wk. Dry seeds, originally 11.2–12.2% moisture content before drying, had low seed respiration, high germination, low levels of most post-harvest molds but also moderate levels of P. verrucosum var. cyclopium and high FAV and therefore were also of low quality. Drying of the seeds occurred too late to prevent initial rapid loss in seed quality caused by seed enzymatic action and Penicillium activity; to be effective, drying of high moisture seeds should be carried out immediately after combining.

scholarly journals Protein, weight, and oil prediction by single-seed near-infrared spectroscopy for selection of seed quality and yield traits in pea (Pisum sativum).

2020 ◽  
Author(s):  
Gokhan Hacisalihoglu ◽  
Jelani Freeman ◽  
Paul R. Armstrong ◽  
Brad W. Seabourn ◽  
Lyndon D. Porter ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Pisum Sativum ◽  
Protein Content ◽  
Near Infrared Spectroscopy ◽  
Seed Weight ◽  
Oil Content ◽  
Near Infrared ◽  
Seed Quality ◽  
Single Seed ◽  
Selection Of

Abstract Background: Pea (Pisum sativum) is a prevalent cool season crop that produces seeds valued for high protein content. Modern cultivars have incorporated several traits that improved harvested yield. However, progress toward improving seed quality has received less emphasis, in part due to the lack of tools for easily and rapidly measuring seed traits. In this study we evaluated the accuracy of single-seed near-infrared spectroscopy (NIRS) for measuring pea seed weight, protein, and oil content. A total of 96 diverse pea accessions were analyzed using both single-seed NIRS and wet chemistry methods. To demonstrate field relevance, the single-seed NIRS protein prediction model was used to determine the impact of seed treatments and foliar fungicides on protein content of harvested dry peas in a field trial. Results: External validation of Partial Least Squares (PLS) regression models showed high prediction accuracy for protein and weight (R2 = 0.94 for both) and less accuracy for oil (R2 = 0.75). Single seed weight was not significantly correlated with protein or oil content in contrast to previous reports. In the field study, the single-seed NIRS predicted protein values were within 1% of an independent analytical reference measurement and were sufficiently precise to detect small treatment effects. Conclusion: The high accuracy of protein and weight estimation show that single-seed NIRS could be used in the dual selection of high protein, high weight peas early in the breeding cycle allowing for faster genetic advancement toward improved pea nutritional quality.

scholarly journals Tetrazolium: an important test for physiological seed quality evaluation

2019 ◽  
Vol 41 (3) ◽  
pp. 359-366 ◽  
Author(s):  
José de Barros França-Neto ◽  
Francisco Carlos Krzyzanowski
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Tetrazolium Salt ◽  
High Quality ◽  
Physiological Quality ◽  
The 19Th Century ◽  
Important Test ◽  
Tetrazolium Test

Abstract: The production of high quality seeds is linked to a good quality control system. In this system, the tetrazolium test plays an important role in the evaluation of the seed physiological quality, not only due to its relative quickness, but also because of the amount of information that the test presents, such as the indexes of viability and vigor, in addition to providing the diagnosis of possible seed quality problems, such as mechanical damage, insect damage, pre-harvest weathering and deterioration during storage. The development of the test had its beginnings at the end of the 19th century, with great evolution in its concepts and methods in the 20th century. For that, there was the dedication of dozens of professionals in several countries, such as Germany, Japan, Russia, Serbia (former Yugoslavia), United States, Argentina and Brazil. The test indirectly measures the respiration processes that occur in the mitochondria of the cells that make up the tissues of the seeds. The reducing reaction of the solution of the tetrazolium salt under the action of dehydrogenase enzymes results in triphenylformazan, which presents a red carmin coloration. By the interpretation of the resulting staining patterns, seed viability, vigor and the main problems affecting seed quality are determined.

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