scholarly journals Seed Quality Evaluation by Tetrazolium Staining of <i>Parkia multijuga</i> Benth

2018 ◽  
Vol 09 (05) ◽  
pp. 577-586 ◽  
Author(s):  
Marcelle Auday Costa ◽  
Elizabeth Santos Cordeiro Shimizu ◽  
Noemi Vianna Martins Leão ◽  
Hugo Alves Pinheiro
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Tetrazolium Staining

scholarly journals Short Communication: Tetrazolium test for evaluating viability of Capsicum annum seeds

2018 ◽  
Vol 10 (3) ◽  
pp. 142-145
Author(s):  
ADITYA KUSUMAWARDANA ◽  
BAMBANG PUJIASMANTO ◽  
PARDONO PARDONO
Keyword(s):  
Plant Growth ◽  
Laboratory Test ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Results ◽  
Capsicum Annum ◽  
Laboratory Test Results ◽  
Tetrazolium Staining ◽  
Growth Vigor ◽  
Tetrazolium Test

Kusumawardana A, Pujiasmanto B, Pardono. 2018. Tetrazolium test for evaluating viability of Capsicum annum seeds. Nusantara Bioscience 10: 142-145. Seed quality is important in seeds production. This research was conducted to obtain information of topographical tetrazolium staining pattern of pepper (Capsicum annum) seeds. Tetrazolium test was conducted to determine the seed viability and plant growth vigor. Laboratory test for standard germination and field performances were performed on four lots (A, B, C, D) of pepper seeds. The viability categories pattern were determined by Root Mean Square (RMS), regression, and correlation analyses. Nine topographical patterns were recognized. The laboratory test results and field performances were compared with the topographical pattern. Combination of patterns 1,2 (embryonic axis and cotyledon completely stained) selected as viable category as it gave the least RMS value, the highest determination (R²) and correlation (r) coefficient with standard germination (RMS = 4, 06; R² = 0,761; r = 0,872). Combination of patterns 1,2 also gave the highest determination (R²) and correlation (r) coefficient with field stand (R² = 0,921; r = 0,959). The combination of patterns 1,2 is recommended for estimating plant growth performance in the field.

scholarly journals Seed Quality Evaluation of Senegalia polyphylla (DC.) Britton & Rose

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Patricia Gibbert ◽  
Michele Fernanda Bortolini ◽  
Daiana Karoline Kaiser ◽  
Evelin Maria Muller ◽  
Francine Gusatto
Keyword(s):  
Quality Evaluation ◽  
Seed Quality

scholarly journals Tetrazolium: an important test for physiological seed quality evaluation

2019 ◽  
Vol 41 (3) ◽  
pp. 359-366 ◽  
Author(s):  
José de Barros França-Neto ◽  
Francisco Carlos Krzyzanowski
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Tetrazolium Salt ◽  
High Quality ◽  
Physiological Quality ◽  
The 19Th Century ◽  
Important Test ◽  
Tetrazolium Test

Abstract: The production of high quality seeds is linked to a good quality control system. In this system, the tetrazolium test plays an important role in the evaluation of the seed physiological quality, not only due to its relative quickness, but also because of the amount of information that the test presents, such as the indexes of viability and vigor, in addition to providing the diagnosis of possible seed quality problems, such as mechanical damage, insect damage, pre-harvest weathering and deterioration during storage. The development of the test had its beginnings at the end of the 19th century, with great evolution in its concepts and methods in the 20th century. For that, there was the dedication of dozens of professionals in several countries, such as Germany, Japan, Russia, Serbia (former Yugoslavia), United States, Argentina and Brazil. The test indirectly measures the respiration processes that occur in the mitochondria of the cells that make up the tissues of the seeds. The reducing reaction of the solution of the tetrazolium salt under the action of dehydrogenase enzymes results in triphenylformazan, which presents a red carmin coloration. By the interpretation of the resulting staining patterns, seed viability, vigor and the main problems affecting seed quality are determined.

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