scholarly journals Determining the Mutagenic Effects of Olive Oil Mill Effluent using the Ames Test

2021 ◽  
Vol 7 (7) ◽  
Author(s):  
Ferhan Korkmaz
Keyword(s):  
Olive Oil ◽  
Ames Test ◽  
Olive Oil Mill Effluent ◽  
Mutagenic Effects

Anaerobic Digestion of Olive Oil Mill Effluent Pretreated and Stored in Municipal Solid Waste Sanitary Landfills

1993 ◽  
Vol 28 (2) ◽  
pp. 27-34 ◽  
Author(s):  
G. Boari ◽  
I. M. Mancini ◽  
E. Trulli
Keyword(s):  
Anaerobic Digestion ◽  
Municipal Solid Waste ◽  
Olive Oil ◽  
Solid Waste ◽  
Pilot Scale ◽  
Methanogenic Activity ◽  
Sanitary Landfills ◽  
Olive Oil Mill Effluent ◽  
A Cell ◽  
Mesh Sieve

Sanitary landfills of municipal solid waste (MSW) might be used to reduce the storage volume required at plants giving year-round treatment of olive oil mill effluent (OME). A landfill in the methanogenic stage could act as an anaerobic filter and reduce the pollutional load of the OME while also acting as a temporary storage tank. In the present work, a lysimeter in pilot scale was used to simulate a cell of a sanitary landfill. It was filled with MSW screened by a 80 mm mesh sieve mixed to municipal sludge. Results show that when OME was spread on the top of the lysimeter at a loading rate not exceeding 0.4 kgCOD/d/m3 of reactor steady methanogenic activity was maintained in the layers of refuse and a 70% removal of COD was obtained in the OME leachate collected. Higher loading rates reduced methanogenic activity and COD removal efficiency. Nevertheless, the OME collected from the bottom of the landfill was more easily treated by anaerobic digestion than was the raw OME.

scholarly journals Sensitivity of plant and bacterial test systems in detecting of mutagenic effects of oil pollution

2006 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
Petimat M Djambetova ◽  
Nina V Reutova
Keyword(s):  
Glycine Max ◽  
Ames Test ◽  
Oil Pollution ◽  
Mutagenic Effect ◽  
Test System ◽  
Test Systems ◽  
Glycine Max L ◽  
Plant Test ◽  
Mutagenic Effects ◽  
By Products

The plant test system soybean (Glycine max. (L.) Merill) line T219 turned out to be more sensitive than standart Ames test for evaluation of the mutagenic effect of soil, contaminated by products of combustion and domestic cottage processing of oil. It is preferable to use plant test systems for such investigations because they are more sensitive, simple and inexpensive in comparison with microbial ones.

Superoxide dismutase protects against paraquat-mediated dioxygen toxicity and mutagenicity: studies in Salmonella typhimurium

1982 ◽  
Vol 60 (11) ◽  
pp. 1367-1373 ◽  
Author(s):  
Hosni M. Hassan ◽  
Carmella S. Moody
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Salmonella Typhimurium ◽  
Normal Level ◽  
Ames Test ◽  
Superoxide Radical ◽  
Oxygen Free Radicals ◽  
Chromosomal Breaks ◽  
Oxygen Sensitive ◽  
Mutagenic Effects

Paraquat is univalently reduced to the relatively stable, but oxygen-sensitive, paraquat radical (PQ∙+). This PQ∙+ can react with dioxygen to generate the superoxide radical, which can further generate other more deleterious species of oxygen free radicals (i.e., hydroxyl radical, OH∙). These oxygen free radicals are known to cause chromosomal breaks; therefore, it was logical to postulate that paraquat is a mutagen. This proved to be the case when tested in a modified Ames test using a liquid incubation assay. Salmonella typhimurium strains TA98 and TA100 were grown in the presence of various concentrations of PQ, as well as in the presence of known mutagenic compounds: mitomycin C, azide, and proflavine. Paraquat was much more toxic and mutagenic in a simple nutritionally restricted medium than in a rich complex medium and these toxic and mutagenic effects were oxygen dependent. Furthermore, cells containing high levels of superoxide dismutase were more resistant to the toxic and mutagenic effects of paraquat than were cells containing a normal level of this enzyme.

scholarly journals Characterization and Quantification of Compounds in the Hydroalcoholic Extract of the Leaves fromTerminalia catappaLinn. (Combretaceae) and Their Mutagenic Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Francisco José Mininel ◽  
Carlos Sérgio Leonardo Junior ◽  
Lívia Greghi Espanha ◽  
Flávia Aparecida Resende ◽  
Eliana Aparecida Varanda ◽  
...  
Keyword(s):  
Ames Test ◽  
Mutagenic Activity ◽  
Complex Mixture ◽  
Calibration Standard ◽  
Hyphenated Techniques ◽  
Hydroalcoholic Extract ◽  
External Calibration ◽  
High Concentrations ◽  
Mutagenic Effects ◽  
C Nmr

Terminaliais a genus of Combretaceous plants widely distributed in tropical and subtropical regions. Thus, the aim of this study was to quantify the majority compounds of the hydroalcoholic extract (7 : 3, v/v) of the leaves fromT. catappaby HPLC-PDA, chemically characterize by hyphenated techniques (HPLC-ESI-IT-MSn) and NMR, and evaluate its mutagenic activity by theSalmonella/microsome assay onS. typhimuriumstrains TA98, TA97a, TA100, and TA102. The quantification of analytes was performed using an external calibration standard. Punicalagin is the most abundant polyphenol found in the leaves. The presence of this compound as a mixture of anomers was confirmed using HPLC-PDA and1H and13C NMR. Mutagenic activity was observed in strains TA100 and TA97a. As the extract is a complex mixture of punicalagin, its derivatives, and several other compounds, the observed mutagenicity may be explained in part by possible synergistic interaction between the compounds present in the extract. These studies show that mutagenic activity ofT. catappain the Ames test can only be observed when measured at high concentrations. However, considering the mutagenic effects observed forT. catappa, this plant should be used cautiously for medicinal purposes.

Genotoxicity of Aqueous Extract From Heated Cooking Oils and its Suppression by Lactobacilli

2009 ◽  
Vol 15 (3) ◽  
pp. 267-273 ◽  
Author(s):  
M. Isidori ◽  
A. Parrella
Keyword(s):  
Ames Test ◽  
Corn Oil ◽  
Sos Response ◽  
Smoke Point ◽  
Genotoxic Effects ◽  
Sos Chromotest ◽  
Genotoxic Activity ◽  
Cooking Oils ◽  
Mutagenic Effects ◽  
Double Heat Treatment

In the present study the mutagenic and genotoxic effects of aqueous extracts from six cooking oils (extra vergine olive, peanut, sunflower, soybean, corn, and various seeds oils) heated to the respective smoke point were investigated. The Ames test and the SOS Chromotest were carried out for this evaluation. The same oils were also tested after their re-frying, simulating domestic reuse process. Furthermore, the ability of different lactobacilli to reduce the potential genotoxic activity of the fried and re-fried oils was determined applying SOS Chromotest after co-incubation of samples with lactobacilli. The results showed that all the fried oils did not produce mutagenic effects while they induced a SOS response with the highest induction factor for the corn oil. Double heat-treatment caused an increase of the genotoxic activity until two times the first heating. The most susceptible oil to the re-frying procedure was the sunflower oil. The antigenotoxicity results were expressed as percent of genotoxicity inhibition. All the tested strains of lactobacilli exhibited antigenotoxic properties on the fried oils.

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