scholarly journals Aerobic Bacterial Isolates and Their Antibiotic Susceptibility Patterns of Pus Samples at Tertiary Healthcare Center of Northern India

2021 ◽  
Vol 8 (9) ◽  
pp. 247-251
Author(s):  
Aditya Rana ◽  
Veehteeanveshna Gupta ◽  
Subhash Chand Jaryal

Introduction: Emerging resistance in microorganisms is a matter of great concern in health care setups. Bacterial isolate and AMR pattern changes from place to place and time to time. Unprofessional use of antibiotics has resulted in an increasing burden of antibiotic resistance. This study focused over the aerobic bacteriological profile and antibiotic resistance pattern in pus isolates. Materials and Methods: A retrospective study was carried out from June, 2020 to May 2021. 1174 pus samples collected during the study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37°C for 18-24 hrs, organisms were identified by biochemical reactions, gram staining and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. Result: Among the total 1174 pus samples, 712 (60.6%) were male and 462 (39.3%) were females. 857(72.9%) samples showed positive results. Among them, Staphylococcus aureus was the most common isolate followed by Escherichia coli, Pseudomonas spp. and Klebsiella spp. The sensitivity of Staphylococcus aureus was highest with Vancomycin and Linezolid, whereas Enterobacterales and Pseudomonas were sensitive with Piperacillin tazobactam. Conclusion: Increasing resistance always poses a challenge in treating infections. The development of resistant strains of pathogens can be limited by the judicious use of antibiotics. This study shows the infecting bacterial isolates and their antibiogram from this area. Keywords: Pus, antimicrobial resistance, bacterial isolates.

2020 ◽  
Vol 14 (3) ◽  
pp. 1961-1966
Author(s):  
A. Deboral ◽  
Namrata K. Bhosale ◽  
S. Umadevi

The antibiotic resistance pattern was observed significantly in various geographical locations. Routine surveillance is therefore essential for constant monitoring of AMR rates in the clinically important pathogens. It is imperative to track the changing resistance pattern over time, to guide proper therapeutic strategies to combat infections due to drug-resistant pathogens. This study aims to highlights the distribution of aerobic bacterial isolated from pus samples, and their susceptibility to different antibiotics collected during 2017 (July to December) in a tertiary care hospital. Nearly 637 clinical pus samples were received during July to December 2017 to the Department of Microbiology, Tertiary care hospital, Puducherry. Bacterial identification was performed using standard conventional biochemical tests and antibiotic susceptibility was carried out according to CLSI guidelines 2017 on each one of the aerobic bacterial isolates from the pus samples. Among the isolates 76.5% were Gram-negative bacilli (GNB) as well as 23.5% were Gram-positive cocci (GPC). The most common bacteria isolated were Pseudomonas spp 24.88% (108 in 434), followed by Escherichia coli 21.66% (94 in 434), Staphylococcus aureus 19.82% (86 in 434) and Klebsiella pneumoniae 13.13% (57 in 434). Of the 86 (19.82%) Staphylococcus aureus isolates, 16 (18.40%) were MRSA. Pseudomonas aeruginosa was highly susceptible to the carbapenems and least susceptible to ciprofloxacin. Acinetobacter baumannii was the most resistant organism according to this study and showed the least susceptibility to ceftriaxone and maximum susceptibility to aminoglycosides. This study concluded that the Pseudomonas aeruginosa isolate was found to be a predominant in our clinical pus samples. Gram negative bacteria are more commonly associated with the pyogenic lesion that Gram positive. A high level of an antibiotic resistance was observed in most of our bacterial isolates.


2017 ◽  
Vol 15 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Rabina Basnet ◽  
Sudarshan Sharma ◽  
Jid Chani Rana ◽  
Pradeep Kumar Shah

Background: Otitis Media is a prevailing and notorious infection in developing countries causing serious local damage and threating complication. Mainly in developing countries like Nepal, Otitis Media results because of illiteracy, poverty and poor hygiene. The aim of this study was to determine the profile of Otitis Media, its causative agents and their antibiotic susceptibility pattern.Methods: The study included 263 pus samples from 240 patients attending ENT department of Bharatpur hospital from May 2015 to January 2016. Samples were processed in microbiology department for bacteria using standard operating protocol. Antibiotic susceptibility testing was performed for all bacterial isolates by Kirby Bauer disc diffusion method and the results were interpreted according to clinical and laboratory standard institute (CLSI) guideline.Results: Out of 240 patients, 121 were female and 119 were male. Highest incidence of Otitis Media was observed in 1-10 year age group. Out of 263 samples taken from 240 patients, 216 showed bacterial growth. Gram negative bacteria predominated and the most common bacteria isolated were Staphylococcus aureus 36.11% followed by Pseudomonas aeruginosa 33.33% and Coagulase Negative Staphylococci 8.08%. All bacterial isolates were sensitive to gentamycin. Staphylococcus aureus was sensitive to Amikacin and gentamycin. All gram negative bacterial isolates were sensitive to Imipenem and gentamycin. 100% of Pseudomonas aeruginosa was sensitive to Imipenem.Conclusions: Staphylococcus aureus was the most predominant organism isolated from the pus swab followed by Pseudomonas aeruginosa and all the isolated organisms were sensitive to Gentamycin.


2020 ◽  
Vol 1 (2) ◽  
pp. 66-83
Author(s):  
I. S. Obuekwe ◽  
C. K. Offodile

  Cattle dung is used as organic fertilizer and alternative source of fuel or biogas but could also be a source of antibiotic resistance genes in the environment. This study isolated, identified and assessed antibiotic susceptibility pattern of bacteria from cattle dung and its contaminated soil. Bacteria isolation and identification were based on standard techniques while hemolytic activity was used to confirm potential pathogenic bacteria. Antibiotic susceptibility pattern of isolated pathogens were assayed by disk diffusion method. Among isolated bacteria, Staphylococcus spp had highest occurrence of 23.8 % while Micrococcus spp was the least at 1.3 %. Hemolytic bacteria isolates were Staphylococcus aureus (16.5 %), Bacillus spp (17.4 %), Nocardia spp (4.6 %), Escherichia coli (29.4 %), Pseudomonas spp (13.8 %), Serratia marcersens (2.8 %) and Salmonella spp (15.6 %). High resistance (100 %) against Ampiclox (30 µg) was observed in all Staphylococcus aureus and Bacillus spp isolates while Pseudomonas aeruginosa isolates showed 100 % resistance to Ofloxacin (30 µg). Most Gram-positive bacterial isolates were majorly resistant to Beta lactams while Gram negative bacteria were resistant to Fluoroquinolones antibiotics. Multiple antibiotics resistant index (MARI) was measured at greater than 0.2, and was observed in 71.5 % of the hemolytic pathogens. Antibiotics resistance in hemolytic bacterial pathogens from this study is indicative of environmental sources of antibiotic resistance and possible adverse effects on human health.


2021 ◽  
Vol 8 (4) ◽  
pp. 160-166
Author(s):  
Dominic Agbo Oche ◽  
Paul Ifeleke Oyegoke ◽  
Maryloveth Oluchukwu Akpudo ◽  
Busayo Olalekan Olayinka

Background and aims: Staphylococcus aureus, an important pathogen in bone diseases, is a highly multi-drug resistant (MDR) bacterium. This study aimed to investigate the antibiotic resistance among S. aureus isolated from patients on admission in an orthopaedic hospital. Methods: In this cross-sectional research, 140 samples comprising urine samples, wound swabs, and nasal swabs were collected from 49 patients on admission. Samples were cultured and screened for S. aureus following standard procedures. Using the agar-disk diffusion method, the isolates were subjected to antibiotics susceptibility tests. Results: S. aureus were isolated from 26 (18.6%) samples, and wound swabs were found to have the highest number of the S. aureus isolates with 12 (46.2%). Among the 26 S. aureus isolated, 25 (96.2%) isolates were resistant to at least four or more of the tested antibiotics. There were 23 (88.5%) MDR isolates, while there were only 2 (7.6%) extensively drug resistant ones. The number of methicillin-resistant S. aureus were 17 (65.4% of the isolates), while the number of methicillin-susceptible S. aureus were 9 (34.6% of the isolates). A total of 22 (84.6%) isolates had multi-antibiotic resistance (MAR) index greater than 0.2. Inducible clindamycin resistance of 2 (7.6%) was observed. Conclusion: This study showed that the S. aureus isolated from the patients were resistant to multiple antibiotics. Regular surveillance of antibiotic resistance is of utmost importance, since it facilitates the design or development of the treatment regimens that could check the spread of antimicrobial resistance.


2020 ◽  
Vol 1 (2) ◽  
pp. 1-11
Author(s):  
S. G. Pandukur ◽  
T. T. Sambo ◽  
A. G. Plangnan

This study was carried out to determine the frequency and antibiotic susceptibility profile of bacterial agents associated with diabetic patients attending out-patients Department in Plateau Specialist Hospital Jos. Wound swabs and pus were collected from the wounds of 251 out-patients’ department (OPD) over a 14 months’ period. Subjects were recruited upon approval by the Ethical Research Committee of the UCTH and after obtaining written or oral informed consent from the subject. Samples were cultured and microbial isolates identified using standard microbiology methods.  Antibiotic susceptibility testing was carried out on the bacterial isolates. Six bacterial isolates were identified from wounds of diabetic patients viz: Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The most frequently encountered bacterial pathogen of wound infection among subjects was Pseudomonas aeruginosa (26.67%) followed by Staphylococcus aureus (23.33%) while the lowest was Klebsiella pneumonia (6.67%). The antibiotic susceptibility profile of the bacterial isolates to commonly used antibiotics ranged between 0.0% to 100%. The flouroquinolones were the most active antimicrobial agents observed among bacterial isolates from diabetic out-patients studied. S. aureus [7(100%) and 6(85.7%)] was the highest susceptible bacteria followed by P. aeruginosa [7(87.5%) and 5(62.5%)], E. coli 6(100%) against Ciprofloxacin (10%) and Augmentin (30%) respectively. Meanwhile, K. pneumonia and S. pneumonia were not susceptible each (0.0%) against Doxycycline and Chloramphenicol at 10% and 30% concentrations respectively. The study showed a high index of wound contamination with bacteria pathogens and resistance pattern to standard and commonly used antibacterial agents among diabetic out-patient from the study area. Pandukur, S. G. | Department of Science Laboratory Technology, Faculty of Natural Sciences, University of Jos.


2014 ◽  
Vol 8 (12) ◽  
pp. 1511-1517 ◽  
Author(s):  
Solmaz Ohadian Moghadam ◽  
Mohammad Reza Pourmand ◽  
Farzaneh Aminharati

Introduction: Burns are the most serious forms of trauma and a major cause of mortality worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common pathogens of burn wound infections; treatment has faced serious problems due to antibiotic resistance in these strains. Biofilm formation, which increases antibiotic resistance capabilities and is considered to be a virulence factor, also causes treatment failure and recurrent staphylococcal infections in burn patients. Methodology: A total of 135 pus/wound swabs were collected; S. aureus was identified by confirmatory tests. The icaA/D and mecA genes were detected in DNA extracts by polymerase chain reaction assay separately. To determine the prevalence of biofilm formation, a modified Congo red agar and the microtiter plate method were used. Investigation of antibiotic resistance was performed using the disk diffusion method. Results: S. aureus (48.87%) was identified in 65 (48.87%) samples, of which 40 (61.53%) were confirmed to be MRSA. Among MRSA and methicillin-sensitive S. aureus (MSSA) isolates, 97.5% and 60% produced biofilm, respectively. Resistance of MRSA isolates to amikacin, ceftriaxone, ciprofloxacin, erythromycin, gentamicin, mupirocin, rifampin, tetracycline, and tobramycin was 64.1%, 76.92%, 51.28%, 87.18%, 71.8%, 10.26%, 5.13%, 89.74%, and 61.54%, respectively. All MRSA and MSSA isolates were susceptible to fusidic acid, linezolid, teicoplanin, tigecycline, and vancomycin. Conclusions: The high prevalence of biofilm-producing, drug-resistant S. aureus isolates in our study suggests that epidemiological studies on the characteristics of common strains found in burn centers and a definition of their antibiotic resistance pattern would be helpful for therapeutic decisions.


2021 ◽  
Author(s):  
Gururaj Kumaresan ◽  
Chetna Gangwar ◽  
Anil Kumar Mishra ◽  
Ashok Kumar ◽  
Suresh Dinkar Kharche ◽  
...  

Abstract Staphylococcus aureus is one of the most prevalent pathogens, and a causative agent of a variety of infections in humans and animals. A total of 48 semen samples were collected from healthy bucks of different breeds to investigate the prevalence of S. aureus . Antimicrobial resistance and virulence of the Staphylococcus isolates were determined to assess the adverse effects of them on buck fertility. The bacterial isolates were tentatively confirmed as Staphylococcus spp. based on the Gram’s staining, growth on Mannitol salt agar and catalase test. Overall, 75% (n = 36) of the samples were positive for Staphylococcus spp. from the total 48 buck semen ejaculates from different breeds. Out of 36 staphylococcal isolates, 23 (47.92%) were coagulase negative (CoNS) and 13 (27.08%) were coagulase positive Staphylococcus (CoPS) based on the slide coagulase test. In the current study, on the basis of molecular characterization, we identified S. aureus , S. chromogenes, S. haemolyticus, S. sciuri, S. simulans and S. epidermidis amongst the staphylococcal isolates in the buck semen. This study revealed a high prevalence of Staphylococcus species in semen of the healthy bucks. The isolates exhibited varying degrees of multidrug resistance genotypically as well as phenotypically. The presence of antibiotic resistance and virulence genes may pose a potential threat to reproductive health of animals, highlighting the need for vigilant monitoring of these isolates at the time of semen cryopreservation.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Sanjeep Sapkota ◽  
Sujan Khadka ◽  
Sanjib Adhikari ◽  
Ashish Parajuli ◽  
Hemraj Kandel ◽  
...  

Background. Motorcycle helmets can serve as a potential vehicle for the transmission of pathogenic bacteria and fungi with serious health implications. The main aim of this study was to explore the microbial diversity associated with the motorcycle helmets and determine the antibiotic susceptibility profile of the bacterial isolates. Methods. A descriptive cross-sectional study was carried out among the teaching staffs of Birendra Multiple Campus, Bharatpur, Nepal. A total of 130 motorcycle helmets worn by the teaching staffs of the Birendra Multiple Campus, Bharatpur, were included in the study for microbiological investigations. Results. Of the total 130 motorcycle helmets analyzed, 392 bacteria and 346 fungi belonging to seven different genera were recovered. Staphylococcus aureus 89 (22.7%) was the predominant bacteria followed by S. epidermidis 77 (19.6%) and E. coli 54 (13.8%), whereas Aspergillus niger 67 (19.4%) was the predominant fungi followed by A. fumigatus 49 (14.2%). Antibiotic susceptibility test was performed by the disc diffusion method for all the bacterial isolates. Tetracycline, gentamycin, and cotrimoxazole were the most effective antibiotics for Gram-positive isolates, whereas Gram-negative isolates were sensitive towards imipenem and ciprofloxacin. Of the total bacterial isolates, 153 (39.0%) were multidrug-resistant (MDR), 10.4% were extended-spectrum beta-lactamase (ESBL) producers, and 4.3% were metallo-beta-lactamase (MBL) producers and, out of 89 isolates of Staphylococcus aureus, 30 (33.7%) were detected as methicillin-resistant Staphylococcus aureus (MRSA). Conclusion. The findings suggest that motorcycle riders should follow good hygiene practices and regularly clean their helmets with suitable sterilants to avoid the risk of microbial contamination and reduce the associated risks.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Bizuneh Tsehayneh ◽  
Taddesse Yayeh ◽  
Birhan Agmas

Background. Staphylococcus aureus is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. Therefore, this study was conducted to assess the bacterial load and antibiotic resistance profile of S. aureus from ready-to-eat raw beef in Bahir Dar city, Ethiopia. Methodology. This cross-sectional study was conducted from October 2018 to April 2019 by collecting a total of 101 raw beef samples from butcher shops using a simple random sampling method. Isolation and microbial load determination of S. aureus use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller–Hinton agar. The data were analyzed by using STATA software version 12.0. Result. Out of 101 raw beef samples, 55 (54.45%) were positive for S. aureus with a mean bacterial count of 3.40 ± 0.63 (log10 cfu/g). About 13% of butcher shops had unacceptable and potentially dangerous (above 104 cfu/g) bacterial load. High S. aureus drug resistance was observed on penicillin (92.73%) followed by cefoxitin (74.5%), tetracycline (63.63%), and clindamycin (50.9%). On the other hand, there was the highest susceptibility for ciprofloxacin (100%) followed by gentamycin (90.91%) and erythromycin (87.27%). Multidrug resistance was also found in 54 (98%) of the isolates. Conclusion. In this study highly drug-resistant S. aureus was incriminated as the main meat contaminant in butcheries of Bahir Dar city. Therefore, appropriate antimicrobial use and staphylococcal control methods should be employed to prevent S. aureus intoxications in foods.


Author(s):  
Razia Khatoon ◽  
Shameem Ahmad Khan ◽  
Noor Jahan

Background: Osteomyelitis is a common cause of morbidity in developing countries. Its treatment comprises of surgical debridement of all necrotic bone and soft tissue along with use of appropriate antimicrobial therapy. Treatment is becoming increasingly troublesome due to rise in drug resistant isolates in osteomyelitis cases. The present study was done to determine the antibiotic resistance pattern among aerobic bacterial isolates from osteomyelitis cases.Methods: 125 samples from osteomyelitis cases were aerobically cultured and isolates from culture positives were identified by standard procedures. Antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method. Staphylococcal isolates were screened for methicillin resistance and Gram negative bacilli were screened and confirmed for ESBL, AmpC and MBL production.Results: Out of 125 samples cultured, 20 were culture negative and 105 were culture positive giving rise to 120 isolates (58 Gram positive and 62 Gram negative organisms). The prevalence of methicillin resistant staphylococcal (MRS) isolates, ESBL, AmpC and MBL producers was found to be 43.1%, 51.6%, 24.2% and 14.5% respectively. All the resistant isolates were multidrug resistant, with MRS being 100% sensitive only to vancomycin, linezolid and teicoplanin, ESBL and AmpC producers being 100% sensitive only to imipenem and colistin, and MBL producers being 100% sensitive only to colistin.Conclusions: Antibiotic therapy on the basis of antibiotic susceptibility pattern helps the clinician to choose appropriate drugs leading to successful treatment and prevention of emergence and dissemination of drug resistant isolates.


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