scholarly journals Early And Late Effects of Radiotherapy on Total Protein Levels of Saliva

1991 ◽  
Vol 1 (2) ◽  
pp. 127-132
Author(s):  
Ayfer Haydaroğlu ◽  
Mustafa Esassolak ◽  
Fatma Kutay ◽  
Nurselen Toygar ◽  
Sevtap Günbay
Keyword(s):  
Late Effects ◽  
Total Protein ◽  
Protein Levels

The Effects of Vitamin K1 and Warfarin on Prothrombin Expression in Human Hepatoblastoma (HepG2) Cells

1992 ◽  
Vol 68 (01) ◽  
pp. 040-047 ◽  
Author(s):  
C Scott Jamison ◽  
Bryan F Burkey ◽  
Sandra J Friezner Degen
Keyword(s):  
Total Protein ◽  
Hepg2 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Response Analysis ◽  
Secreted Protein ◽  
Mrna Levels ◽  
Vitamin K1 ◽  
Maximal Increase ◽  
Protein Levels ◽  
Warfarin Treatment

SummaryCultures of human hepatoblastoma (HepG2) cells were treated with vitamin K1 or warfarin and prothrombin antigen and mRNA levels were determined. With 3 and 6 h of 10 µg vitamin K1 treatment secreted prothrombin antigen levels, relative to total secreted protein levels, were increased 1.5-fold and 2.1-fold, respectively, over ethanol-treated control levels as determined by an enzyme-linked immunosorbent assay. Dose-response analysis with 3 h of 25 µg/ml vitamin K1 treatment demonstrated a maximal increase of 2.0-fold in secreted prothrombin antigen levels, relative to total secreted protein levels, over ethanol-treated control levels. Pulse-chase analysis with 35S-methionine and immunoprecipitation of 35S-labelled prothrombin demonstrated that, with vitamin K1 treatment (25 µg/ml, 3 h), the rate of prothrombin secretion increased approximately 2-fold and the total amount (intra- and extracellular) of prothrombin synthesized increased approximately 50% over ethanol-treated control levels. Warfarin treatment (1, 5, or 10 µg/ml, 24 h) resulted in decreases in secreted prothrombin antigen levels, relative to total protein levels to approximately 85%, 87% or 81% of ethanol-treated control levels. Analysis of total RNA isolated from these cultures by Northern and solution hybridization techniques demonstrated that prothrombin mRNA was approximately 2.1 kb and that neither vitamin K1 nor warfarin treatment affected the quantity of prothrombin mRNA (ranging from 240–350 prothrombin mRNA molecules per cell). These results demonstrate that vitamin K1 and warfarin, in addition to effects on γ-carboxylation, affect prothrombin synthesis post-transcriptionally, perhaps influencing translation, post-translational processing and/or secretion mechanisms.

Age-related changes in renal and hepatic cellular mechanisms associated with variations in rat serum thyroid hormone levels

2008 ◽  
Vol 294 (6) ◽  
pp. E1160-E1168 ◽  
Author(s):  
Elena Silvestri ◽  
Assunta Lombardi ◽  
Pieter de Lange ◽  
Luigi Schiavo ◽  
Antonia Lanni ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Total Protein ◽  
Protein Level ◽  
Monocarboxylate Transporter ◽  
Type I ◽  
Peripheral Tissues ◽  
Protein Levels ◽  
Age Related ◽  
Liver And Kidney ◽  
Age Related Changes

Aging is associated with changes in thyroid gland physiology. Age-related changes in the contribution of peripheral tissues to thyroid hormone serum levels have yet to be systematically assessed. Here, we investigated age-related alterations in the contributions of the liver and kidney to thyroid hormone homeostasis using 6-, 12-, and 24-mo-old male Wistar rats. A significant and progressive decline in plasma thyroxine occurred with age, but triiodothyronine (T3) was decreased only at 24 mo. This was associated with an unchanged protein level of the thyroid hormone transporter monocarboxylate transporter 8 (MCT8) in the kidney and with a decreased MCT8 level in the liver at 24 mo. Hepatic type I deiodinase (D1) protein level and activity declined progressively with age. Renal D1 levels were decreased at both 12 and 24 mo but D1 activity was decreased only at 24 mo. In the liver, no changes occurred in thyroid hormone receptor (TR) TRα1, whereas a progressive increase in TRβ1 occurred at both mRNA and total protein levels. In the kidney, both TRα1 and TRβ1 mRNA and total protein levels were unchanged between 6 and 12 mo but increased at 24 mo. Interestingly, nuclear TRβ1 levels were decreased in both liver and kidney at 12 and 24 mo, whereas nuclear TRα1 levels were unchanged. Collectively, our data show differential age-related changes among hepatic and renal MCT8 and D1 and TR expressions, and they suggest that renal D1 activity is maintained with age to compensate for the decrease in hepatic T3 production.

scholarly journals Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries

2016 ◽  
Vol 19 (1) ◽  
pp. 71-76 ◽  
Author(s):  
L Koç Öztürk ◽  
A Yarat ◽  
S Akyuz ◽  
H Furuncuoglu ◽  
K Ulucan
Keyword(s):  
Molecular Weight ◽  
Oral Hygiene ◽  
Total Protein ◽  
Turkish Population ◽  
Dental Students ◽  
Oral Bacteria ◽  
World Health ◽  
Low Molecular Weight ◽  
Coding Region ◽  
Protein Levels

ABSTRACTHuman low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding.

scholarly journals Relation between both oxidative and metabolic-osmotic cell damages and initial injury severity in bombing casualties

2006 ◽  
Vol 63 (6) ◽  
pp. 545-551 ◽  
Author(s):  
Marina Vuceljic ◽  
Gordana Zunic ◽  
Predrag Romic ◽  
Miodrag Jevtic
Keyword(s):  
Total Protein ◽  
Injury Severity ◽  
Venous Blood ◽  
Early Period ◽  
Cellular Membrane ◽  
Protein Levels ◽  
Oxidative Damages ◽  
Gradual Development ◽  
Initial Injury ◽  
Initial Severity

Background/Aim. We have recently reported the development of oxidative cell damages in bombing casualties within a very early period after the initial injury. The aim of this study, was to investigate malondialdehyde (MDA), as an indicator of lipid peroxidation, and osmolal gap (OG), as a good indicator of metabolic cell damages and to assess their relationship with the initial severity of the injury in bombing casualties. Methods. The study included the males (n = 52), injured during the bombing with the Injury Severity Score (ISS) ranging from 3 to 66. The whole group of casualties was devided into a group of less severely (ISS < 25, n = 24) and a group of severely (ISS ? 26, n = 28) injured males. The uninjured volunteers (n = 10) were the controls. Osmolality, MDA, sodium, glucose, urea, creatinine, total bilirubin and total protein levels were measured in the venous blood, sampled daily, within a ten-day period. Results. In both groups of casualties, MDA and OG levels increased, total protein levels decreased, while other parameters were within the control limits. MDA alterations correlated with ISS (r = 0.414, p < 0.01), while a statistically significant correlation between OG and ISS was not obtained. Interestingly, in spite of some differences in MDA and OG trends, at the end of the examined period they were at the similar level in both groups. Conclusion. The initial oxidative damages of the cellular membrane with intracellular metabolic disorders contributed to the gradual development of metabolic-osmotic damages of cells, which, consequently caused the OG increase. In the bombing casualties, oxidative cell damages were dependent on the initial injury severity, while metabolic-osmotic cell damages were not.

scholarly journals Склад крові та його зв'язок із молочною продуктивністю корів різного віку та рівня продуктивності

2013 ◽  
pp. 85-88
Author(s):  
Є. Ф. Ткач
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Total Protein ◽  
Morphological Study ◽  
Direct Proportion ◽  
Bovine Blood ◽  
Positive Correlation ◽  
Black And White ◽  
Protein Levels

Викладено результати дослідження біохімічного і морфологічного складу крові корів української чорно-рябої молочної і голштинської порід та його зв’язку з молочною продуктивністю. Досліджено, що рівень молочної продуктивності пов’язаний або знаходиться у прямій залежності від інтенсивності обмінних процесів в організмі тварин. Встановлено позитивний кореляційний зв’язок між загальним білком крові і надоєм корів та між альбумінами і надоєм. Не виявлено закономірного взаємозв’язку між кількістю лейкоцитів і молочною продуктивністю корів обох порід. The article presents the results of biochemical and morphological study of bovine blood of Ukrainian black and white dairy and Holstein breeds and its relationship with milk production. It has been proved that the level of milk production is associated or is in direct proportion to the intensity of metabolism in animals. The positive correlation between total protein levels and milk yield of cows and between albumin and milk yield has been stated. No regular relationship between the number of leukocytes and milk production of cows of both breeds has been revealed.

Influence of pneumatic tube system transport on routinely assessed and spectrophotometric cerebrospinal fluid parameters

2017 ◽  
Vol 55 (1) ◽  
pp. 47-52
Author(s):  
Pavel Broz ◽  
Daniel Rajdl ◽  
Jaroslav Racek ◽  
Jana Zenkova ◽  
Vlasta Petrikova
Keyword(s):  
Total Protein ◽  
Mononuclear Cells ◽  
Turnaround Time ◽  
Protein Levels ◽  
Glucose Levels ◽  
Pneumatic Tube ◽  
Clinically Significant ◽  
Lactate Levels ◽  
Biochemical Assessment ◽  
Fluid Parameters

Abstract Background: Pneumatic tube systems (PTS) are widely used in many hospitals. Using PTS reduces turnaround time (TAT) and can improve patients’ outcome. Methods: We investigated whether clinically significant differences could be observed in CSF samples transported by pneumatic tube in comparison with samples transported by hand. Two aliquots from one sample were sent by PTS and by hand from the department of neurology or neurosurgery and compared. Results: Routine cytological and biochemical assessment was compared in 27 cases. There were no statistically significant changes (transport by hand vs. PTS) in glucose levels [data are expressed as median (minimum–maximum)] at 3.7 (2.5–8.6) mmol/L vs. 3.6 (2.7–8.6) mmol/L, p=0.96 or lactate levels at 1.8 mmol/L (1.1–5.5) vs. 1.8 mmol/L (1.1–5.4). We observed a statistically significant decline in total protein levels in samples transported by PTS at 0.56 g/L (0.19–4.29) vs. 0.49 g/L (0.18–4.3), p=0.008. We observed no changes in erythrocyte count at 5/μL (0–40,000) vs. 5/μL (0–40,106), mononuclear cells at 2/μL (1–145) vs. 3/μL (1–152), or polynuclear cells at 0/μL (0–235) vs. 0/μL (0–352). Spectrophotometric examination was performed in 20 cases. There were no statistically significant differences (transport by hand vs. transport by PTS) in NOA at 0.002 (0.001–1.537) vs. 0.001 (0.001–1.528), p=0.95 or NBA at 0.001 (0.001–0.231) vs. 0.001 (0.001–0.276), p=0.675. Samples transported by PTS were delivered faster than samples transported by courier (transport by hand vs. PTS) at 25 min (10–153) vs. 15 min (4–110), p=0.002. Conclusions: We found no significant changes in glucose, lactate levels and in any of the cytological parameters assessed, nor were statistically significant changes observed in the spectrophotometric parameters. We found a statistically significant decrease in total protein levels in samples transported by PTS. Transport by PTS can be faster than transport by hand.

EVALUATION OF MEAT IN THE INFANT DIET

PEDIATRICS ◽  
1952 ◽  
Vol 10 (4) ◽  
pp. 463-473
Author(s):  
HOWARD M. JACOBS ◽  
GEORGE S. GEORGE
Keyword(s):  
Total Protein ◽  
Infant Nutrition ◽  
Physical Growth ◽  
Morbidity Rate ◽  
Control Group ◽  
Average Weight ◽  
Protein Requirements ◽  
Protein Levels ◽  
Young Infants ◽  
Significant Difference

A commercially prepared lean meat was fed to young infants as a supplement to a diet already adequate in all food factors. In those infants first fed meat under 2 months of age, there was improvement in physical growth as determined by weight and height measurements. The same group demonstrated an improvement in hemoglobin levels; the elimination of the physiologic drop in total protein levels of the serum, with a prompt sustained rise in values, the greater part of which was composed of the globulin fraction. In those infants first fed meat after 2 months of age, there was a slight improvement in average weight gain, but no significant difference was noted in other body measurements, total protein, albumin or globulin fractions, or hemoglobin levels. Illness rates for the two years of the study demonstrated a 40% lower morbidity rate in the meat-fed group as compared to the control group. In striving for the optimum in infant nutrition protein requirements should be evaluated both quantitatively and qualitatively.

Neuroprotective Effects of the Anthocleista Schweinfurthii Gilg. (Loganiaceae) Stem Bark Extract in Postmenopause-Like Model of Ovariectomized Wistar Rats

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Ngoungoure Madeleine Chantal ◽  
Dzeufiet Djomeni Paul Désiré ◽  
Bilanda Danielle Caude ◽  
Mengue Ngandena Yolande Sandrine ◽  
Mbolang Nguegang Lohik ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Plant Extract ◽  
Total Protein ◽  
Wistar Rats ◽  
Stem Bark ◽  
Bark Extract ◽  
Estradiol Valerate ◽  
Neuroprotective Effects ◽  
Protein Levels ◽  
Stem Bark Extract

Abstract Background Estrogen deficiency in postmenopausal period causes severe neuroendocrine changes in brain which influences memory and other nervous functions. Anthocleista schweinfurthii is used traditionally to treat female infertility and menopause related symptoms. This study was performed to investigate the potential neuroprotective effects of aqueous extract of Anthocleista schweinfurthii on brain in a postmenopause-like model of ovariectomized Wistar rats. Methods Thirty animals were sham-operated or ovariectomized (Ovx) 84 days after surgery, six groups of five rats each were daily treated orally during 28 days with: distilled water for groups 1 (sham-operated) and 2 (Ovx), estradiol valerate (group 3) and the three doses of extracts {groups 4, 5 and 6 (Ovx)}. Biochemical and histological evaluations focused on brain. Results Compared to sham-operated control, ovariectomy decreased total protein levels in brain (p<0.01) which was increased by plant extract at the dose of 300 mg/kg (p<0.05), underlying its anabolic properties. Ovariectomy significantly decreased magnesium levels in brain (p<0.001). Anthocleista schweinfurthii increased significantly magnesium levels (p<0.01), showing its capacity to act on synaptic conduction. Ovariectomy induced oxidative stress by increasing malondialdehyde levels (p<0.05) and decreasing reduced glutathione levels (p<0.05) in brain. The plant extract exhibited antioxidative activity by reducing malondialdehyde levels and increasing glutathione levels in brain. Damage in brain structure which was caused by ovariectomy disappeared following the treatment. Conclusions Results suggest that Anthocleista schweinfurthii may have neuroprotective effects in Ovx Wistar rats by increasing total protein, magnesium levels and reducing oxidative stress in brain.

scholarly journals Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling

2018 ◽  
Vol 62 (4) ◽  
pp. 485-492 ◽  
Author(s):  
Barbara Kazuń ◽  
Joanna Małaczewska ◽  
Krzysztof Kazuń ◽  
Joanna Żylińska-Urban ◽  
Andrzej K. Siwicki
Keyword(s):  
Lactobacillus Plantarum ◽  
Common Carp ◽  
Total Protein ◽  
Bacterial Infections ◽  
Control Diet ◽  
Head Kidney ◽  
Dietary Supplementation ◽  
Protein Levels ◽  
Killing Activity ◽  
The Common

AbstractIntroduction: Immune-potentiating functions of Lactobacillus plantarum strains in the common carp were evaluated.Material and Methods: Fourteen days of feeding fish dry diet supplemented with the bacteria provided parameters of nonspecific humoral immunity (lysozyme, ceruloplasmin, γ-globulin, total protein levels, and serum bactericidal activity) and cellular immunity (pinocytosis, respiratory burst activity, and potential killing activity of organ phagocytes), as well as the proliferative response of organ lymphocytes stimulated with mitogens. The resistance of fish to infection with Aeromonas hydrophila was also determined.Results: Dietary supplementation with L. plantarum had a substantial influence on the activity of organ phagocytes, especially the potential killing activity of head kidney cells. A significant increase in the proliferative activity of LPS-stimulated B lymphocytes and in the levels of γ-globulins and total protein was observed. The supplemented diet conveyed higher resistance than the control diet as the cumulative fish mortalities after infection with A. hydrophila were 65% and 85%, respectively.Conclusion: The results indicate that dietary supplementation with L. plantarum stimulates the antibacterial resistance of common carp and may reinforce defence against bacterial infections, but further studies need to be conducted.

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