scholarly journals Prevalence of H pylorii In The Tonsillar Tissue of The Children With Giemsa Stain.

2018 ◽  
Author(s):  
Ali Kurt ◽  
Rabia Demirtaş ◽  
Hilal Balta ◽  
Şenay Erdoğan Durmuş ◽  
Emre Akarsu
Keyword(s):  
Giemsa Stain ◽  
Tonsillar Tissue

Changes in tonsillar tissue in early HIV-1 infection and during 3 years of antiretroviral therapyNote

Apmis ◽  
2000 ◽  
Vol 108 (7-8) ◽  
pp. 539-550 ◽  
Author(s):  
PAL VOLTERSVIK ◽  
ANNE MA DYRHOL-RIISE ◽  
LEIF BOSTAD ◽  
BARD I. ROSOK ◽  
JAN OLOFSSON ◽  
...  
Keyword(s):  
Tonsillar Tissue ◽  
Hiv 1

PD-L1 Immunohistochemistry on Cell Block Preparations From Different Fixatives: A Pilot Study

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S95-S95
Author(s):  
Victoria Costa ◽  
David Kim ◽  
Abha Goyal ◽  
Bing He ◽  
Yifang Liu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Personalized Medicine ◽  
Cell Block ◽  
Fixation Methods ◽  
Tonsillar Tissue ◽  
Fixed Cell ◽  
Specific Fixation ◽  
Formalin Fixed

Abstract Objectives Immunohistochemistry especially for biomarkers such as PD-L1 in personalized medicine is increasingly being performed on cell blocks from cytopathology samples. We evaluated the effects of staining cell blocks (CBs) from different cytologic fixatives using four different commercially available PD-L1 antibody clones. Methods PD-L1 immunohistochemistry (IHC) using four different commercially available clones was performed on eight cell blocks processed from two different fixatives and also on eight formalin-fixed tissues. The clones used were 22C3 at 1:50 dilution and 1:100 dilution, SP263, SP142, and E1L3N. The cell block (CB) samples contained cells that strongly express PD-L1 and either fixed in CytoLyt (methanol-based fixative, n = 4) or CytoRich (ethanol-formalin-based fixative, n = 4). Formalin-fixed control tissues (tonsillar tissue, n = 4; stomach, n = 4) were also included. Results Expression for PD-L1 was noted on all the CytoRich fixed cell blocks (n = 4) for all four antibody clones, with the intensity and distribution of expression comparable to the formalin fixed tissues (n = 8). However, consistent absence of expression for PD-L1 was noted on all the CytoLyt fixed cell blocks (n = 4) for all four antibody clones. Conclusion The results of this pilot study demonstrate that PD-L1 IHC on cell blocks is feasible. However, there is a need to validate IHC protocols according to specific fixation methods.

scholarly journals Microbiology of parapharyngeal abscesses in adults: in search of the significant pathogens

2021 ◽  
Author(s):  
Tejs Ehlers Klug ◽  
Thomas Greve ◽  
Camilla Andersen ◽  
Pernille Hahn ◽  
Christian Danstrup ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Relative Abundance ◽  
Maximum Growth ◽  
Fusobacterium Necrophorum ◽  
Adult Patients ◽  
Tonsillar Tissue ◽  
Streptococcus Anginosus ◽  
Parapharyngeal Abscess ◽  
Bacterial Cultures

AbstractWe aimed to describe the microbiology of parapharyngeal abscess (PPA) and point out the likely pathogens using the following principles to suggest pathogenic significance: (1) frequent recovery, (2) abundant growth, (3) growth in relative abundance to other microorganisms, (4) percentage of the isolates recovered in both absolute and relative abundance, (5) more frequent recovery in PPA pus compared with tonsillar surface and tissue. Comprehensive bacterial cultures were performed on specimens obtained from adult patients (n = 60) with surgically verified PPA, who were prospectively enrolled at five Danish ear-nose-throat departments. The prevalent isolates (in PPA pus) were unspecified anaerobes (73%), non-hemolytic streptococci (67%), Streptococcus anginosus group (SAG) (40%), Corynebacterium spp. (25%), Neisseria spp. (23%), Fusobacterium spp. (22%), Fusobacterium necrophorum (17%), Prevotella spp. (12%), and Streptococcus pyogenes (10%). The bacteria most frequently isolated in heavy (maximum) growth were unspecified anaerobes (60%), SAG (40%), F. necrophorum (23%), and Prevotella spp. (17%). The predominant microorganisms (those found in highest relative abundance) were unspecified anaerobes (53%), SAG (28%), non-hemolytic streptococci (25%), F. necrophorum (15%), S. pyogenes (10%), and Prevotella spp. (10%). Four potential pathogens were found in both heavy growth and highest relative abundance in at least 50% of cases: F. necrophorum, Prevotella spp., SAG, and S. pyogenes. SAG, Prevotella spp., F. necrophorum, S. pyogenes, and Bacteroides spp. were recovered with the same or higher frequency from PPA pus compared with tonsillar tissue and surface. Our findings suggest that SAG, F. necrophorum, Prevotella, and S. pyogenes are significant pathogens in PPA development.

scholarly journals Staining Interactions of Bixa Orellana (Annatto) Seed Extracts with Tissues of Wistar Rats

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Fasogbon Samuel Ayobami
Keyword(s):  
Column Chromatography ◽  
Wistar Rats ◽  
Blood Smear ◽  
Wistar Rat ◽  
Ethanol Solution ◽  
Giemsa Stain ◽  
Staining Reaction ◽  
Distilled Water ◽  
Bixa Orellana ◽  
Seed Extracts

Introduction: Bixa orellana is an ancestral multi use plant popularly known as Achiote or lipstick tree in view of its reddish – orange dye on its seeds, Central and South American populations used these seeds to color their bodies and lips, B. orellana is the only species of Bixaceae family. Aim: The aim of this study was to stain the organs such as skin, liver, kidney, brain, intestines, and bone marrow of a wistar rats with solutions of Bixa orellana (Annatto) seed extracts. Methods: The study design was experimental research, the seeds of Bixa orellana were extracted in distilled water, absolute ethanol and acetone, using maceration methods and the fractions of the extracts were determined using column chromatography while preliminary, the solutions of the extracts were used to stain tissues listed above at varying pH to establish staining interactions of the crude extracts and fractions eluted from the column chromatography. Results: The most significant staining interaction were observed in ethanol solution of bixa extract, followed by acetone and distilled water and staining were also observed at column fraction(9-26), with background yellowish appearance in all the staining solutions which was contrast to controls (haematoxylin and eosin) stained sections and no staining reaction was observed in blood smear of wistar rat. The staining interactions of great quality was observed at pH 5.0 and 7.5, despite its staining ability it was neither superior to eosin nor a substitute to giemsa stain due to the yellowish background appearance and its complete inactivity on blood smear. Conclusion: Bixa orellana dye extracts can be grouped under compound dyes or neutral dyes due to its interaction at both pH 5.0 and 7.5 and cannot be used as substitute for Giemsa stain.

scholarly journals Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies

2020 ◽  
Vol 27 (5) ◽  
pp. 53-61
Author(s):  
Abdullah Saleh Alkhamiss
Keyword(s):  
Helicobacter Pylori ◽  
Alcian Blue ◽  
National Guard ◽  
Periodic Acid ◽  
Giemsa Stain ◽  
Periodic Acid Schiff ◽  
Gastric Biopsies ◽  
Hematoxylin And Eosin ◽  
H Pylori ◽  
Sensitivity Specificity

Background: This study was undertaken to evaluate the preferred method (Giemsa or periodic acid Schiff-Alcian blue [PAS-AB] stains) of detecting Helicobacter pylori (H. pylori) in gastric mucosal biopsies in terms of sensitivity, specificity and applicability. To the best of my knowledge, this is the first report comparing Giemsa and PAS-AB staining for the detection of H. pylori in such biopsies. Methods: The formalin-fixed paraffin-embedded blocks of 49 gastric biopsies from different patients were collected from the archive of anatomical pathology at King Abdulaziz Medical City, National Guard, Riyadh, Saudi Arabia. From each block, three slides were prepared and analysed using the hematoxylin and eosin (H&E), Giemsa and PAS-AB stains to detect the presence/absence of H. pylori, and the results were compared in terms of sensitivity, specificity and applicability. Results: The majority of the biopsies in this study showed antrum-type gastric mucosa. Only 15 biopsies showed active gastritis, whereas the rest showed chronic gastritis. Three biopsies showed intestinal metaplasia. All were detected by PAS-AB stain, but only two-thirds were detected by H&E stain. Fifteen gastric biopsies showed H. pylori infection in general and in 13 of them, active gastritis cases were discovered. Fourteen out of these 15 H. pylori infection cases were detected by Giemsa stain, whereas only 13 cases were detected by H&E stain. PAS-AB stain showed the worst results since it demonstrated only 40% sensitivity and 67.65% specificity in H. pylori detection. Conclusion: Giemsa stain has better sensitivity and specificity in gastric H. pylori infection detection than PAS-AB. Therefore, using PAS-AB stain to detect H. pylori infection is not recommended.

scholarly journals Isolation of Mononuclear Cells from Tonsillar Tissue

2009 ◽  
Vol 86 (1) ◽  
Author(s):  
Andrew Johnston ◽  
Sigrun L. Sigurdardottir ◽  
Judith J. Ryon
Keyword(s):  
Mononuclear Cells ◽  
Tonsillar Tissue

scholarly journals Multiple cutaneous mast cell tumours in a Boa imperator

2020 ◽  
Vol 8 (1) ◽  
pp. e001040
Author(s):  
Ann-Kathrin Onkels ◽  
Christina Stadler ◽  
Udo Hetzel ◽  
Jana Mueller ◽  
Christiane Herden
Keyword(s):  
Mast Cell ◽  
Subcutaneous Tissue ◽  
Skin Lesions ◽  
Giemsa Stain ◽  
Cell Populations ◽  
Round Cell ◽  
Dense Granules ◽  
The Family ◽  
Cutaneous Mast Cell ◽  
Metachromatic Granules

Mast cell tumours (MCTs) are common in dogs and cats, but are only rarely reported in reptiles. This case documents the clinical and pathomorphological results from a Boa imperator with multiple cutaneous nodules, diagnosed as MCT based on histopathology, immunohistochemistry and electron microscopy approaches. Grossly, there were multifocal, poorly demarcated, mostly ulcerated nodules ~3 cm in diameter on the skin. Histologically, the dermis and the subcutaneous tissue were infiltrated by round cell populations with eosinophilic granules. Toluidine blue and Giemsa stain revealed metachromatic granules. Using immunohistochemistry, some cells exhibited cytoplasmic immunostaining positive for tryptase. Ultrastructurally, variable quantities of intracytoplasmic, spherical and electron-dense granules were also detected. The MCT literature on snakes is scarce, especially for the family Boidae, but MCTs should be considered a differential diagnosis for nodular skin lesions in reptiles.

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