Tonsillar Tissue

2020 ◽  
Author(s):  
Keyword(s):  
Tonsillar Tissue

Changes in tonsillar tissue in early HIV-1 infection and during 3 years of antiretroviral therapyNote

Apmis ◽  
2000 ◽  
Vol 108 (7-8) ◽  
pp. 539-550 ◽  
Author(s):  
PAL VOLTERSVIK ◽  
ANNE MA DYRHOL-RIISE ◽  
LEIF BOSTAD ◽  
BARD I. ROSOK ◽  
JAN OLOFSSON ◽  
...  
Keyword(s):  
Tonsillar Tissue ◽  
Hiv 1

PD-L1 Immunohistochemistry on Cell Block Preparations From Different Fixatives: A Pilot Study

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S95-S95
Author(s):  
Victoria Costa ◽  
David Kim ◽  
Abha Goyal ◽  
Bing He ◽  
Yifang Liu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Personalized Medicine ◽  
Cell Block ◽  
Fixation Methods ◽  
Tonsillar Tissue ◽  
Fixed Cell ◽  
Specific Fixation ◽  
Formalin Fixed

Abstract Objectives Immunohistochemistry especially for biomarkers such as PD-L1 in personalized medicine is increasingly being performed on cell blocks from cytopathology samples. We evaluated the effects of staining cell blocks (CBs) from different cytologic fixatives using four different commercially available PD-L1 antibody clones. Methods PD-L1 immunohistochemistry (IHC) using four different commercially available clones was performed on eight cell blocks processed from two different fixatives and also on eight formalin-fixed tissues. The clones used were 22C3 at 1:50 dilution and 1:100 dilution, SP263, SP142, and E1L3N. The cell block (CB) samples contained cells that strongly express PD-L1 and either fixed in CytoLyt (methanol-based fixative, n = 4) or CytoRich (ethanol-formalin-based fixative, n = 4). Formalin-fixed control tissues (tonsillar tissue, n = 4; stomach, n = 4) were also included. Results Expression for PD-L1 was noted on all the CytoRich fixed cell blocks (n = 4) for all four antibody clones, with the intensity and distribution of expression comparable to the formalin fixed tissues (n = 8). However, consistent absence of expression for PD-L1 was noted on all the CytoLyt fixed cell blocks (n = 4) for all four antibody clones. Conclusion The results of this pilot study demonstrate that PD-L1 IHC on cell blocks is feasible. However, there is a need to validate IHC protocols according to specific fixation methods.

scholarly journals Microbiology of parapharyngeal abscesses in adults: in search of the significant pathogens

2021 ◽  
Author(s):  
Tejs Ehlers Klug ◽  
Thomas Greve ◽  
Camilla Andersen ◽  
Pernille Hahn ◽  
Christian Danstrup ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Relative Abundance ◽  
Maximum Growth ◽  
Fusobacterium Necrophorum ◽  
Adult Patients ◽  
Tonsillar Tissue ◽  
Streptococcus Anginosus ◽  
Parapharyngeal Abscess ◽  
Bacterial Cultures

AbstractWe aimed to describe the microbiology of parapharyngeal abscess (PPA) and point out the likely pathogens using the following principles to suggest pathogenic significance: (1) frequent recovery, (2) abundant growth, (3) growth in relative abundance to other microorganisms, (4) percentage of the isolates recovered in both absolute and relative abundance, (5) more frequent recovery in PPA pus compared with tonsillar surface and tissue. Comprehensive bacterial cultures were performed on specimens obtained from adult patients (n = 60) with surgically verified PPA, who were prospectively enrolled at five Danish ear-nose-throat departments. The prevalent isolates (in PPA pus) were unspecified anaerobes (73%), non-hemolytic streptococci (67%), Streptococcus anginosus group (SAG) (40%), Corynebacterium spp. (25%), Neisseria spp. (23%), Fusobacterium spp. (22%), Fusobacterium necrophorum (17%), Prevotella spp. (12%), and Streptococcus pyogenes (10%). The bacteria most frequently isolated in heavy (maximum) growth were unspecified anaerobes (60%), SAG (40%), F. necrophorum (23%), and Prevotella spp. (17%). The predominant microorganisms (those found in highest relative abundance) were unspecified anaerobes (53%), SAG (28%), non-hemolytic streptococci (25%), F. necrophorum (15%), S. pyogenes (10%), and Prevotella spp. (10%). Four potential pathogens were found in both heavy growth and highest relative abundance in at least 50% of cases: F. necrophorum, Prevotella spp., SAG, and S. pyogenes. SAG, Prevotella spp., F. necrophorum, S. pyogenes, and Bacteroides spp. were recovered with the same or higher frequency from PPA pus compared with tonsillar tissue and surface. Our findings suggest that SAG, F. necrophorum, Prevotella, and S. pyogenes are significant pathogens in PPA development.

scholarly journals Isolation of Mononuclear Cells from Tonsillar Tissue

2009 ◽  
Vol 86 (1) ◽  
Author(s):  
Andrew Johnston ◽  
Sigrun L. Sigurdardottir ◽  
Judith J. Ryon
Keyword(s):  
Mononuclear Cells ◽  
Tonsillar Tissue

The Relevance of Increased Tonsil Uptake When Restaging Lymphoma with Positron Emission Tomography

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3133-3133
Author(s):  
Javier Munoz ◽  
Belisario Arango ◽  
Jessica Schering ◽  
James Morrison ◽  
Ishani Dalal ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Conflicts Of Interest ◽  
Standardized Uptake Value ◽  
Emission Tomography ◽  
Invasive Approach ◽  
Fdg Uptake ◽  
Tonsillar Tissue ◽  
Positron Emission ◽  
Lymphomatous Involvement ◽  
The Mean

Abstract Abstract 3133 Introduction: Significant tonsil uptake is sometimes observed in F-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) after treatment. Some patients undergo tonsillectomy or FNA (fine needle aspiration) to rule out malignant involvement particularly when management depends on restaging (Figure 1). Our study describes the incidence and degree of tonsil FDG uptake in a large group of lymphoma patients that underwent PET scanning. Patients and Methods: Single institution, retrospective chart review by ICD code, 617 lymphoma patients that underwent PET at our institution from 2004 to 2009. Results of PET were compared to pathological diagnosis of tonsillectomy (Table 1) or FNA biopsy (Table 2) when available which was performed at physician's discretion. Patients that were diagnosed with lymphoma during restaging studies performed for a different primary malignancy were excluded. Results and Discussion: All 8 tonsillectomies and FNA biopsies performed after a restaging PET that showed increased tonsil uptake were negative for malignancy (Figure 2). All of these 8 patients had an initial previous PET that did not show increased tonsil uptake and also these 8 patients remained in remission from their lymphoma after the procedure was performed. In contrast, 6 out of 7 patients that underwent tonsillectomy or FNA at diagnosis were positive for malignancy (Table 3). Differences among tonsil FDG uptake has been thought to reflect differences in activity of “physiological” inflammation of the palatine tonsils. Increased glucose metabolism during active inflammation in the case of chronic tonsillitis or lymphocyte proliferation in the case of a patient that has received prior chemotherapy (likely experiencing compensatory extra medullar lymphoid hyperplasia) were thought to be causes of high FDG uptake in the tonsils. The significance of such increased tonsil FDG uptake is currently unknown however previous studies suggest that normal pharyngeal palatine tonsil uptake was generally symmetrical and that the difference in maximal standardized uptake value (SUVmax) between right and left tonsils (right-to-left ratio or a surrogate of symmetry) in the same patient might be helpful in detecting malignant tissue. The mean right-to-left ratio of tonsillar SUV was 4.55 in patients with confirmed malignant pathology and 1.53 in patients with documented benign tonsillar tissue (Table 4). The mean tonsillar SUVmax was 15.35 in patients with confirmed malignant pathology and 7.05 in patients with documented benign tonsillar tissue hence SUVmax seems to be useful in differentiating tumor from physiological accumulation. Younger patients with low SUV max symmetric tonsillar uptake and no other abnormal FDG areas seen during restaging PET could probably be watched non-invasively. Conclusions: At the time of initial staging PET, increased tonsil uptake showed true lymphomatous involvement in most cases. At restaging, increased tonsil uptake displayed no cases positive for lymphomatous involvement as all tonsillectomies and FNA biopsies were negative for malignancy. These findings seem to be valid irrespective of the subtype of lymphoma. Our study supports a conservative non-invasive approach because physiologic uptake is the most common cause of increased tonsil uptake when restaging lymphoma patients after treatment has been completed. Disclosures: No relevant conflicts of interest to declare.

Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease

2006 ◽  
Vol 35 (3) ◽  
pp. 292-297 ◽  
Author(s):  
Jari Suvilehto ◽  
Merja Roivainen ◽  
Mikko Seppänen ◽  
Seppo Meri ◽  
Tapani Hovi ◽  
...  
Keyword(s):  
Tonsillar Tissue

Penetration of erythromycin into tonsillar tissue

1985 ◽  
Vol 9 (9) ◽  
pp. 611-615 ◽  
Author(s):  
M. Falchi ◽  
F. Teodori ◽  
A. Carraro ◽  
C. Cioce ◽  
F. Scaglione ◽  
...  
Keyword(s):  
Tonsillar Tissue
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