scholarly journals METHODS FOR FISH SPECIES IDENTIFICATION IN FOOD PRODUCTS

10.5219/25 ◽  
2010 ◽  
Vol 4 (3) ◽  
Author(s):  
Pavol Bajzík ◽  
Jozef Golian ◽  
Radoslav Židek ◽  
Jozef Čapla ◽  
Ľubomír Belej ◽  
...  
Keyword(s):  
Species Identification ◽  
Fish Species ◽  
Food Products ◽  
Fish Species Identification

scholarly journals METHODS OF MOLECULAR DIAGNOSTICS FOR FISH SPECIES IDENTIFICATION

Food systems ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 32-41
Author(s):  
T. A. Fomina ◽  
V. Yu. Kornienko ◽  
M. Yu. Minaev
Keyword(s):  
Species Identification ◽  
Fish Species ◽  
Dna Analysis ◽  
Food Products ◽  
The Body ◽  
Morphological Identification ◽  
Polymorphism Analysis ◽  
Technical Regulation ◽  
Fish Products ◽  
Fish Species Identification

The growth in demand for fish products as a result of globalization of trade caused a risks and threats of selling poor-quality and falsified fish products. This has become a great problem both for supervising agencies and for consumers.Many countries have regulations on food labelling and safety. For example, in the Russian Federation, Republic of Belarus and Republic of Kazakhstan has been passed the Technical Regulation of the Customs Union TR CU022/2011 “Food products in part of their labeling” that aims to prevent misinformation of consumers to ensuring realization of consumer rights to reliable information about food products, and Technical Regulation TR EAEU040/2016 “On safety of fish and fish products” requires indication of the zoological name of the species of the aquatic biological resource or the object of aquaculture.Fish species identification is traditionally carried out based on external morphological traits. However, it becomes impossible to identify species by ichthyological traits upon fish cutting, if the head and fins are removed, and the body is cut on pieces (especially, in case of fillets) and even more so upon technological processing. In this case, objective analytical methods of species identification are used, which are based on ELISA or PCR. However, DNA‑based methods have several advantages compared to ELISA methods and complement traditional morphological identification methods. This paper gives a wide overview of the most recent and used methods of fish species identification based on DNA analysis such as single-strand conformation polymorphism (SSCP) analysis, species-specific PCR, real-time PCR, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), DNA barcoding, Sanger sequencing and next-generation sequencing (NGS).

scholarly journals Environmental DNA (eDNA) Metabarcoding in the Fish Market and Nearby Seafood Restaurants in Taiwan Reveals the Underestimation of Fish Species Diversity in Seafood

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1132
Author(s):  
Hung-Tai Lee ◽  
Cheng-Hsin Liao ◽  
Te-Hua Hsu
Keyword(s):  
Species Diversity ◽  
Species Identification ◽  
Fish Species ◽  
Large Scale ◽  
Environmental Dna ◽  
Universal Primers ◽  
Similar Species ◽  
Operational Taxonomic Units ◽  
Fish Market ◽  
Fish Species Identification

Seafood, especially in traditional food Taiwan, is rarely sourced from a fixed species and routinely from similar species depending on their availability. Hence, the species composition of seafood can be complicated. While a DNA-based approach has been routinely utilized for species identification, a large scale of seafood identification in fish markets and restaurants could be challenging (e.g., elevated cost and time-consuming only for a limited number of species identification). In the present study, we aimed to identify the majority of fish species potentially consumed in fish markets and nearby seafood restaurants using environmental DNA (eDNA) metabarcoding. Four eDNA samplings from a local fish market and nearby seafood restaurants were conducted using Sterivex cartridges. Nineteen universal primers previously validated for fish species identification were utilized to amplify the fragments of mitochondrial DNA (12S, COI, ND5) of species in eDNA samples and sequenced with NovaSeq 6000 sequencing. A total of 153 fish species have been identified based on 417 fish related operational taxonomic units (OTUs) generated from 50,534,995 reads. Principal Coordinate Analysis (PCoA) further showed the differences in fish species between the sampling times and sampling sites. Of these fish species, 22 chondrichthyan fish, 14 Anguilliformes species, and 15 Serranidae species were respectively associated with smoked sharks, braised moray eels, and grouper fish soups. To our best knowledge, this work represents the first study to demonstrate the feasibility of a large scale of seafood identification using eDNA metabarcoding approach. Our findings also imply the species diversity in traditional seafood might be seriously underestimated and crucial for the conservation and management of marine resources.

Rapid Fish Species Identification by Agarose Gel Isoelectric Focusing of Sarcoplasmic Proteins

1981 ◽  
Vol 64 (1) ◽  
pp. 38-43
Author(s):  
Ronald C Lundstrom
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Protein Pattern ◽  
Agarose Gel ◽  
Tissue Fluid ◽  
Protein Patterns ◽  
Fish Species Identification ◽  
Species Specific ◽  
Gel Isoelectric Focusing

Abstract A rapid method is described for fish species identification by agarose gel isoelectric focusing (AGIEF). The AGIEF method can be completed in less than 2 h and gives reproducible species-specific sarcoplasmic protein patterns. Protein patterns are similar using either centrifuged tissue fluid or muscle tissue as the sample. One species, monkfish (Lophius americanus), has a polymorphic protein pattern. A predominant pattern was found in 66.7% of the individuals; 2 variant patterns were equally distributed among the remaining 33.3%. AGIEF offers a more rapid, less expensive alternative to the current AOAC official first action method for fish species identification based on polyacrylamide gel isoelectric focusing.

Collaborative Study of a Rapid Electrophoretic Method for Fish Species Identification

1969 ◽  
Vol 52 (4) ◽  
pp. 703-707
Author(s):  
Robert J Learson
Keyword(s):  
Cellulose Acetate ◽  
Species Identification ◽  
Fish Species ◽  
Collaborative Study ◽  
Correct Identification ◽  
Protein Patterns ◽  
Electrophoretic Method ◽  
Fish Species Identification ◽  
Fish Samples ◽  
Standard Protein

Abstract A rapid electrophoretic method for fish species identification, using cellulose acetate as the supporting medium, was collaboratively studied to determine whether photographs of standard protein patterns from authentic species could he used to identify unknown samples. Twelve collaborators were sent six unknown samples of fish in duplicate to identify from a set of photographs representing standard patterns from nine species of fish. Results obtained from the 10 reporting collaborators indicated that correct identification from photographic standards was extremely difficult. Although the analysts were able to match the duplicates with an accuracy of 90%, only 39% of the unknowns were correctly identified. It is recommended that the method be collaboratively studied using authentic fish samples for standards instead of photographic standards.

Fish species identification by isoelectric focusing of parvalbumins in immobilized pH gradients

1996 ◽  
Vol 17 (8) ◽  
pp. 1380-1385 ◽  
Author(s):  
Josep S. Esteve-Romero ◽  
Ingrid Malmheden Yman ◽  
Alessandra Bossi ◽  
Pier Giorgio Righetti
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Ph Gradients ◽  
Fish Species Identification
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