scholarly journals Effects of the administration of brewed Robusta coffee leaves on total antioxidant status in rats with high-fat, high-fructose diet-induced metabolic syndrome

10.5219/1350 ◽  
2020 ◽  
Vol 14 ◽  
pp. 258-263
Author(s):  
Nurmasari Widyastuti ◽  
Gemala Anjani ◽  
Vita Gustin Almira ◽  
Suci Eka Putri ◽  
Amali Rica Pratiwi
Keyword(s):  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Coffea Canephora ◽  
Control Group ◽  
High Fat ◽  
Robusta Coffee ◽  
High Fructose ◽  
Total Antioxidant ◽  
Phytochemical Compounds

Robusta coffee (Coffea canephora) leaves contain phytochemical compounds and have antioxidant and anti-diabetic effects. This study investigated the effect of brewed Robusta coffee leaves on the total antioxidant status in metabolic syndrome rats. Metabolic syndrome in rats was induced by high-fat-fructose diet containing pork oil (20%), cholesterol (1.5%), cholic acid (0.5%), standard feed (80%), and fructose (1 mL per 200 g BW). The animals were categorized into normal control group (K1), metabolic syndrome control group without treatment (K2), mangiferin treatment group (X1), brewed Robusta coffee leaves 0.09 g per 200 BW group (X2), brewed Robusta coffee leaves 0.18 g per 200 BW group (X3), and brewed Robusta coffee leaves 0.36 g per 200 BW group (X4). Each dose of the coffee leaves was brewed with 3.6 mL of water at 70 °C for 10 min. The intervention was administered for 28 days. There was a significant increase in the total antioxidant status (p <0.000) in all the groups. In conclusion, the administration of brewed Robusta coffee leaves increased the total antioxidant status in metabolic syndrome rats.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals Total Antioxidant Status and Other Markers to Distinguish Severely Obese Volunteers with and without Metabolic Syndrome

2019 ◽  
Vol 10 (06) ◽  
pp. 648-663
Author(s):  
Sharmistha Singh ◽  
Astha Dwivedi ◽  
Sandeep Kumar ◽  
Poonam Chandra Mittal
Keyword(s):  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Total Antioxidant ◽  
Severely Obese

scholarly journals Negative association between paraoxonase 2, anthropometric markers and metabolic syndrome

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Andrada Mihai ◽  
Daniela Lixandru ◽  
Petruta Alexandru ◽  
Irina Stoian ◽  
Simona Carniciu ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Mononuclear Cells ◽  
Total Antioxidant Status ◽  
Peripheral Blood Mononuclear ◽  
Mortality And Morbidity ◽  
Type 2 Diabetic Mellitus ◽  
Total Antioxidant ◽  
Antioxidant Markers ◽  
Anthropometric Markers

AbstractBackground Metabolic syndrome (MS) has a great impact on cardiovascular mortality and morbidity. Our aim was to investigate the association of MS with some oxidant and antioxidant markers, including pro-and antioxidant status of peripheral blood mononuclear cells (PBMC) in newly diagnosed type 2 diabetic mellitus patients (ND-T2D). Methods 219 ND-T2D and 88 healthy subjects were divided in two groups according to the absence or presence of MS. Anthropometric measurements, routine blood tests, total oxidant status (TOS), total antioxidant status (TAS) and ELISA measurements were included. The PBMC capacity to release free radicals and to neutralize them was also determined by measuring the respiratory burst (RB) together with the lactonase activity of the intracellular antioxidant enzyme paraoxonase 2 (PON2). Results Comparing ND-T2D MS+ with those MS- the RB of the PBMC was significantly higher (p<0.05) while lactonase PON2 enzymatic activity was decreased (p < 0.001). A negative correlation of RB was found with TAS (r = -0.416, p < 0.05). PON2 was also negatively correlated with glycaemia (r = -0.275, p < 0.001), HbA1c (r = -0.308, p < 0.001), weight (r = -0.183; p < 0.05), waist circumference (r = -0.353, p < 0.001) and body mass index (r = -0.290, p < 0.001). Conclusion PON2 lactonase activity is negatively associated with anthropometric markers in ND-T2D with MS.

scholarly journals Dietary Plant Extracts Improve the Antioxidant Reserves in Weaned Piglets

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 702
Author(s):  
Carlo Corino ◽  
Michel Prost ◽  
Barbara Pizzi ◽  
Raffaella Rossi
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Whole Blood ◽  
Antioxidant Status ◽  
Biological Activities ◽  
Total Antioxidant Status ◽  
Dietary Treatment ◽  
Control Group ◽  
Commercial Diet ◽  
Total Antioxidant

Reducing the use of antibiotics in livestock in order to contain antibiotic resistance and studying natural substance additives are key to sustainability. Among the various biological activities of plant extracts, antioxidant activity plays an important role. The present study assesses the total antioxidant activity and antioxidant reserves using the Kit Radicaux Libres test (KRL™ Kirial International, Couternon, France). One hundred and sixty piglets (Topics × Tempo) weaned at 28 days of age were divided into four dietary treatment groups that were fed a commercial diet (the control group, C); 500 mg/kg Boswellia extract (BOS); 200 and 50 mg/kg Uncaria and Tanacetum extracts (UT) respectively; and 225 mg/kg of an antioxidant plant extract mixture (AOX). The blood antioxidant activity of the piglets was measured using the KRL test and the reserves were analyzed on whole blood samples after hydrolysis with glucosidase, sulfatase and glucuronidase. No significant differences were observed in growth performance. The delta KRL values of the whole blood showed a significantly higher total antioxidant status of the piglets from the BOS and AOX groups than the UT and C groups (+30.7 BOS; +27.7 AOX vs. +17.81 UT +13.30 C; p = 0.002) between 18 and 28 days post-weaning. The delta KRL values of red blood cells (RBCs) showed a significantly higher total antioxidant status of the piglets from the AOX groups than the UT and BOS groups (+22.2 AOX; vs. +9.90 UT +9.4 BOS; p = 0.016) between the two sampling times. Reserves of UT and AOX were higher than C and BOS for all enzymes, glucosides, sulphates, and glucuronides. The biological KRL test proved to be an extremely sensitive tool to evaluate the piglets’ antioxidant status. Determining the antioxidant reserve also provides a better understanding of the real antioxidant status of pigs.

scholarly journals Effect of Vitamins A, C, and E Supplementation in the Treatment of Metabolic Syndrome in Albino Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
L. S. Bilbis ◽  
S. A. Muhammad ◽  
Y. Saidu ◽  
Y. Adamu
Keyword(s):  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Total Antioxidant Status ◽  
Lipoprotein Cholesterol ◽  
Albino Rats ◽  
Low Density Lipoprotein Cholesterol ◽  
Salt Diet ◽  
Total Antioxidant

Obesity and metabolic syndrome increase the risk of cardiovascular morbidity and mortality. Oxidative stress seems to be involved in the path physiology of cardiovascular complications of metabolic syndrome. In this study we investigated the effects of vitamins A, C, and E in the management of metabolic syndrome traits condition in albino rats fed with high salt diet. The rats were placed on 8% NaCl diet for 5 weeks and then supplemented with these vitamins for additional 4 weeks in the presence of salt diet. Supplementation with vitamins significantly (P<0.01) decreased blood pressure of the rats as compared with the control. Supplementation also significantly (P<0.05) reduced serum total cholesterol, triglyceride, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol and increased high-density lipoprotein cholesterol, and total antioxidant status as compared with untreated group. The percentage protection of the supplemented groups against atherogenesis indicated55.50±3.75%. Percentage weight gain indicated significant positive correlation with triglyceride, insulin resistance, and malondialdehyde while total antioxidant status and nitric oxide showed significant negative correlation. Salt diet significantly (P<0.05) induced features of metabolic syndrome. The result, therefore, indicated strong relationship between obesity and metabolic syndrome and underscores the role of these vitamins in the management of metabolic syndrome.

scholarly journals Serum Homocysteine and Reactive Species Levels in Fragility Fractures of the Pelvis

2019 ◽  
Vol 70 (9) ◽  
pp. 3216-3219 ◽  
Author(s):  
Alexandru Filip ◽  
Oana Viola Badulescu ◽  
Paul Dan Sirbu ◽  
Elena Cojocaru ◽  
Nina Filip ◽  
...  
Keyword(s):  
Bone Quality ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Fragility Fractures ◽  
Reactive Species ◽  
Control Group ◽  
Serum Homocysteine ◽  
Total Antioxidant ◽  
The Relationship

Although there are studies showing the link between homocysteine and bone quality, the relationship remains controversial. The purpose of the study was to investigate homocysteine levels in patients with fragility fractures of the pelvis and their association with serum Total Antioxidant Status.The study included a group of 60 patients, aged ]65years, with pelvic fragility fractures and osteoporosis admitted in our trauma department between January 2015 and January 2018 and a control group of 30 patients, aged ]65years without fragility fractures or osteoporosis.The total antioxidant status (TAS) and homocysteine (Hcy) concentrations were determined in serum of all the patients. TAS levels had a significantly lower values in the group with pelvic fragility fractures compared with the control group (p[0.001).We noticed a significant higher mean of Hcy levels (p[0.001) in patients with pelvic fragility fractures compared with the control group. As conclusion this study shows that patients with fragility fractures of the pelvis have moderately elevated levels of homocysteine and reduced serum Total Antioxidant Status.

scholarly journals Metabolic Syndrome (MetS) and Nonalcoholic Steatohepatitis (NASH): Study of biochemical markers Free Fatty Acid (FFA), Total Antioxidant Status (TAOS), Adiponectin, Transforming Growth Factor (TGF-beta1), in occurence of NASH

2009 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Agus Sulaeman ◽  
A Rifai Amiruddin ◽  
Gatot Susilo Lawrence
Keyword(s):  
Metabolic Syndrome ◽  
Fatty Acid ◽  
Growth Factor ◽  
Free Fatty Acid ◽  
Fatty Liver ◽  
Antioxidant Status ◽  
Transforming Growth Factor ◽  
Total Antioxidant Status ◽  
Total Antioxidant ◽  
Tgf Β1

BACKGROUND: The prevalence of metabolic syndrome (MetS) in USA and Makassar are 22% and 23.7%. The prevalence of Non Alcoholic Steatosis Hepatosis (NASH) in MetS has not been reported. Study in Non-alcoholic Fatty Liver Disease (NAFLD) is 25–90 % in obesity patients. In NASH, there is accumulation of lipid in hepatocyte (raised free fatty acid level), raised stress oxidative (decreased total antioxidant status), raised of inflammation process (decreased adiponectin) and hepatic fibrotic process (raised TGF β1). The aim of this study is to investigate the correlation of free fatty acid, total antioxidant status, adiponectin and TGF-β1 with the occurrence of NASH.METHODS: This was a case control study in man aged ≥30 years old. Metabolic syndrome (MetS) was defined by IDF categories. NASH was defined as fatty liver plus raised type IV collagen level ≥140 ng/ml and Alanine Transferase (ALT) level 1.5x upper normal limit.RESULT: The samples consisted of 8 MetS subjects, 11 MetS subjects with fatty liver and 2 MetS subjects with suspect NASH. Low level of adiponectin and high level free fatty acid led to progression from Fatty Liver (FL) to NASH. Level of total antioxidant and Level of TGF-β1 were relatively steady in NASH.CONCLUSION: The level of Free Fatty acid in subjects with MetS-FL was higher than in subjects with MetS, but was lower than in subjects with MetS-NASH. No difference in total antioxidants status level was observed among all groups. Level of adiponectin decreased in subjects with MetS-FL and MetS-NASH compared with subjects with MetS only. The level of TGF-β1 increased in subjects with MetS-FL more than in subjects with MetS only, and was steady low in subjects with MetS-NASH.KEYWORDS: metabolic syndrome, NASH, free fatty acid, total antioxidant status, adiponectin, transforming growth factor β1

scholarly journals Behavior of the total antioxidant status in a group of subjects with metabolic syndrome

2014 ◽  
Vol 8 (3) ◽  
pp. 166-169 ◽  
Author(s):  
Eugenia Hopps ◽  
Baldassare Canino ◽  
Maria Montana ◽  
Rosalia Lo Presti ◽  
Maurizio R. Averna ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Total Antioxidant

Oxidative stress increases in carbon monoxide poisoning patients

2010 ◽  
Vol 30 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Havva Sahin Kavakli ◽  
Ozcan Erel ◽  
Orhan Delice ◽  
Gizem Gormez ◽  
Semra Isikoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Antioxidant Status ◽  
Carbon Monoxide Poisoning ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Parameters ◽  
Total Antioxidant ◽  
Oxidant Status

Objective: Carbon monoxide poisoning (COP) is one of the important causes of morbidity and mortality in toxicological cases. In this study, we aimed to find out more about the pathophysiology of COP by investigating the effects of COP on oxidative stress parameters such as total oxidant status (TOS) and total antioxidant status (TAS). Methods: Eighty-eight patients admitted to the emergency department of our hospital with acute COP and 35 healthy adults as control group were included in this study. Blood samples were collected from all COP patients at the time of initial emergency department evaluation to determine the oxidative stress parameters. Then, serum levels of total antioxidant status and total oxidant status levels were measured. Results: A total of 88 patients poisoned by carbon monoxide (CO; mean age 37.1 ± 18.2 years; 54’% women) were enrolled. TOS and carboxyhemoglobin (COHb) levels in COP patients were increased when compared to control group (p = 0.001). TOS, oxidative stress index (OSI) and COHb levels in COP patients were significantly lower after the treatment. (respectively, p = 0.016; p = 0.023; p = 0.001). On the other hand, no statistical differences were observed in TAS levels of study and control group as well as there were no changes with treatment. Conclusion: Measurements of TOS, TAS and OSI levels may be useful markers to find out the pathophsiology of COP.

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