scholarly journals Viabilidade in vitro de leveduras armazenadas à temperatura ambiente

2019 ◽  
Vol 16 (E) ◽  
Author(s):  
Reginaldo Dos Santos Pedroso ◽  
Ralciane De Paula Menezes
Keyword(s):  
Cryptococcus Neoformans ◽  
Candida Spp ◽  
Cryptococcus Spp

Introdução. O armazenamento de isolados fúngicos, especialmente de leveduras, é importante para estudos de sensibilidade a antifúngicos ou outros testes complementares ao diagnóstico laboratorial, especialmente daqueles que ocorrem a partir de sítios como sangue e líquidos de cavidades corporais fechadas. Objetivo. Avaliar a viabilidade de leveduras dos gêneros Candida e Cryptococcus armazenadas em água destilada, solução salina fisiológica e BHI-glicerol, mantidas à temperatura ambiente. Material e Métodos. Foram analisados 404 isolados de leveduras, sendo 354 isolados de espécies de Candida e 50 de Cryptococcus neoformans/gattii. Resultados. Dos isolados de Candida spp. foram recuperados 100/178 (56,2%) isolados a partir de água destilada, 90/97 (92,8%) isolados a partir de solução salina fisiológica e 63/79 (79,7%) a partir do BHI-glicerol. Dos isolados de Cryptococcus spp. armazenados em BHI-glicerol foram recuperados 30/50 (60%) dos isolados. Conclusão. Para laboratórios com pouca demanda em exames micológicos, em que ocorrem poucos isolamentos de leveduras, especialmente do gênero Candida, o armazenamento em solução salina parece ser alternativa melhor que em água destilada.

scholarly journals Primeira descrição da caracterização fenotípica e susceptibilidade in vitro a drogas de leveduras do gênero Cryptococcus spp isoladas de pacientes HIV positivos e negativos, Estado de Mato Grosso

2009 ◽  
Vol 42 (6) ◽  
pp. 661-665 ◽  
Author(s):  
Olivia Cometti Favalessa ◽  
Luciano Correa Ribeiro ◽  
Tomoko Tadano ◽  
Cor Jesus Fernandes Fontes ◽  
Flávio Basili Dias ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Cryptococcus Gattii ◽  
Mato Grosso ◽  
Cryptococcus Spp

Foram avaliados 37 isolados de 10 pacientes HIV negativos e 26 positivos, em Mato Grosso. Exame direto, cultura e quimiotipagem de espécies foram realizados. Cetoconazol, itraconazol, voriconazol, fluconazol e anfotericina B foram avaliados. Foram identificadas 37 leveduras do gênero Cryptococcus spp sendo 26 de pacientes HIV- positivos (25 Cryptococcus neoformans e um Cryptococcus gattii) e 10 de HIV- negativos (cinco Cryptococcus neoformans e cinco Cryptococcus gattii). Considerando isolados clínicos (Cryptococcus neoformans) de HIV positivos observou-se resistência (8% e 8,7%) e susceptibilidade dose-dependência (20% e 17,4%) para fluconazol e itraconazol respectivamente. Para isolados de Cryptococcus neoformans oriundos de pacientes HIV negativos, observou-se susceptibilidade dose-dependência (40%) ao fluconazol. Os isolados de Cryptococcus gattii provenientes de pacientes HIV- negativos mostraram-se susceptíveis a todos os antifúngicos, exceto um isolado de Cryptococcus gattii que foi susceptível dose-dependente ao fluconazol (20%). O isolado proveniente do paciente HIV- positivo demonstrou resistência ao fluconazol (CIM > 256µg/mL) e itraconazol (CIM=3µg/mL).

scholarly journals In vitro Evaluation of the Curcumin against Fluconazole-Resistant Strains of Candida spp. and Cryptococcus neoformans

2018 ◽  
Vol 7 (04) ◽  
pp. 1191-1202
Author(s):  
Daniel Domingues Freitas ◽  
Cecília Rocha da Silva ◽  
João Batista de Andrade Neto ◽  
Rosana de Sousa Campos ◽  
Letícia Serpa Sampaio ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Candida Spp ◽  
In Vitro Evaluation ◽  
Fluconazole Resistant ◽  
Resistant Strains

Antifungal activity of promethazine and chlorpromazine against planktonic cells and biofilms of Cryptococcus neoformans/Cryptococcus gattii complex species

2020 ◽  
Vol 58 (7) ◽  
pp. 906-912
Author(s):  
Raimunda Sâmia Nogueira Brilhante ◽  
Wilker Jose Perez Gotay ◽  
Vandbergue Santos Pereira ◽  
Jonathas Sales de Oliveira ◽  
Waldemiro Aquino Pereira-Neto ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
Fungal Pathogens ◽  
Cryptococcus Gattii ◽  
Planktonic Cells ◽  
Complex Species ◽  
Cryptococcus Spp

Abstract Cryptococcus neoformans/Cryptococcus gattii are fungal pathogens that affect the central nervous system, mainly in immunocompromised individuals. Due to the limited pharmacological arsenal available for the treatment of cryptococcosis associated with cases of antifungal resistance of Cryptococcus spp. reported in some studies, the search for new compounds with antifungal potential becomes relevant. Thus, the objective of this study was to evaluate the inhibitory effect of phenothiazines (promethazine and chlorpromazine) on C. neoformans/C. gattii planktonic cells and biofilms. In vitro planktonic susceptibility testing was performed using the broth microdilution assay. The effect of phenothiazines was evaluated against biofilm formation and mature Cryptococcus biofilms. Biofilm morphology and ultrastructure were also evaluated by scanning electron microscopy. Promethazine and chlorpromazine showed antifungal activity against planktonic cells, with minimum inhibitory concentrations of 8–32 μg/ml and 4–16 μg/ml, respectively. As for biofilm formation, phenothiazines reduced biomass by 60% and metabolic activity by 90% at 64 μg/ml; while in mature biofilms, reductions of 85% and 90% in biomass and metabolic activity, respectively, were observed at 1024 μg/ml. Promethazine and chlorpromazine were also able to disrupt and fragment biofilms. In conclusion, promethazine and chlorpromazine have antifungal activity against planktonic cells and biofilms of Cryptococcus spp. These data show the potential of promethazine and chlorpromazine as antibiofilm drugs.

scholarly journals Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts

2009 ◽  
Vol 42 (2) ◽  
pp. 110-113 ◽  
Author(s):  
João Jaime Giffoni Leite ◽  
Érika Helena Salles Brito ◽  
Rossana Aguiar Cordeiro ◽  
Raimunda Sâmia Nogueira Brilhante ◽  
José Júlio Costa Sidrim ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Hexane Extract ◽  
Candida Spp ◽  
Malassezia Pachydermatis ◽  
Methanol Extracts

The present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate their larvicidal activity towards Aedes aegypti and investigate their in vitro antifungal potential against strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis through the microdilution technique. In toxicity tests on Artemia salina, the hexane and methanol extracts from avocado seeds showed LC50 values of 2.37 and 24.13mg mL-1 respectively. Against Aedes aegypti larvae, the LC50 results obtained were 16.7mg mL-1 for hexane extract and 8.87mg mL-1 for methanol extract from avocado seeds. The extracts tested were also active against all the yeast strains tested in vitro, with differing results such that the minimum inhibitory concentration of the hexane extract ranged from 0.625 to 1.25mg L-¹, from 0.312 to 0.625mg mL-1 and from 0.031 to 0.625mg mL-1, for the strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis, respectively. The minimal inhibitory concentration for the methanol extract ranged from 0.125 to 0.625mg mL-1, from 0.08 to 0.156mg mL-1 and from 0.312 to 0.625mg mL-1, for the strains of Candida spp., Cryptococcus neoformans and Malassezia pachydermatis, respectively.

scholarly journals In-vitro activity of dicationic aromatic compounds and fluconazole against Cryptococcus neoformans and Candida spp.

1999 ◽  
Vol 44 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Maurizio Del Poeta ◽  
Amy S. Bixel ◽  
Francesco Barchiesi ◽  
Richard R. Tidwell ◽  
David Boykin ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Aromatic Compounds ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Candida Spp

scholarly journals Fungal Biofilms as a Valuable Target for the Discovery of Natural Products That Cope with the Resistance of Medically Important Fungi—Latest Findings

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1053
Author(s):  
Estefanía Butassi ◽  
Laura Svetaz ◽  
María Cecilia Carpinella ◽  
Thomas Efferth ◽  
Susana Zacchino
Keyword(s):  
Natural Products ◽  
Essential Oils ◽  
Natural Compounds ◽  
Candida Spp ◽  
Highly Active ◽  
In Vivo Testing ◽  
Brazilian Propolis ◽  
Cryptococcus Spp

The development of new antifungal agents that target biofilms is an urgent need. Natural products, mainly from the plant kingdom, represent an invaluable source of these entities. The present review provides an update (2017–May 2021) on the available information on essential oils, propolis, extracts from plants, algae, lichens and microorganisms, compounds from different natural sources and nanosystems containing natural products with the capacity to in vitro or in vivo modulate fungal biofilms. The search yielded 42 articles; seven involved essential oils, two Brazilian propolis, six plant extracts and one of each, extracts from lichens and algae/cyanobacteria. Twenty articles deal with the antibiofilm effect of pure natural compounds, with 10 of them including studies of the mechanism of action and five dealing with natural compounds included in nanosystems. Thirty-seven manuscripts evaluated Candida spp. biofilms and two tested Fusarium and Cryptococcus spp. Only one manuscript involved Aspergillus fumigatus. From the data presented here, it is clear that the search of natural products with activity against fungal biofilms has been a highly active area of research in recent years. However, it also reveals the necessity of deepening the studies by (i) evaluating the effect of natural products on biofilms formed by the newly emerged and worrisome health-care associated fungi, C. auris, as well as on other non-albicans Candida spp., Cryptococcus sp. and filamentous fungi; (ii) elucidating the mechanisms of action of the most active natural products; (iii) increasing the in vivo testing.

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