scholarly journals Proinflammatory Cytokines in the Saliva, Gingival Crevicular Fluid and Serum of Diabetic Patients with Periodontal Disease

2013 ◽  
pp. 1-10
Author(s):  
Fawad Javed ◽  
Asma Ahmed
Keyword(s):  
Periodontal Disease ◽  
Proinflammatory Cytokines ◽  
Gingival Crevicular Fluid ◽  
Diabetic Patients ◽  
Crevicular Fluid

scholarly journals BETA GLUCURONIDASE ACTIVITY IN DIABETES ASSOCIATED PERIODONTITIS

2020 ◽  
Vol 8 (9) ◽  
pp. 1222-1230
Author(s):  
Gawali S ◽  
◽  
Padhye A ◽  
Chavan P ◽  
◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Enzyme Activity ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Diabetic Patients ◽  
Tissue Destruction ◽  
Pocket Depth ◽  
Glucuronidase Activity ◽  
Probing Pocket Depth ◽  
Crevicular Fluid

Diabetes mellitus is a risk factor for development of periodontal disease with progressive periodontal destruction seen in diabetic patients. Advances in oral and periodontal disease diagnostic research are moving towards methods whereby periodontal risk can be identified and quantified by objective measures such as biomarkers. The aim of this study was to identify the presence of beta glucuronidase activity as an enzymatic biomarker of periodontal tissue destruction in patients of Diabetes mellitus associated with Periodontitis. β Glucuronidase activitywas estimated in saliva, GCF (Gingival Crevicular Fluid) and serum in healthy subjects and patients with Diabetes mellitus, Periodontitis and Diabetes associated periodontitis. Enzyme activity was compared with clinical parameters like Probing pocket depth, Plaque Index and Gingival Index. It was maximally raised in diabetic cases with periodontitis. Enzyme activity was found to be highest in crevicular fluid compared to saliva and serum. However, serum Glucuronidase correlated significantly with clinical indices. β-glucuronidase may be employed on routine basis as a chair side test for screening and diagnosis of patients with periodontitis in diabetics.

scholarly journals Influence of Gestational Hormones on the Bacteria-Induced Cytokine Response in Periodontitis

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Betsaida J. Ortiz-Sánchez ◽  
Martha Legorreta-Herrera ◽  
Miriam Rodriguez-Sosa
Keyword(s):  
Periodontal Disease ◽  
Proinflammatory Cytokines ◽  
Gingival Crevicular Fluid ◽  
Bacterial Biofilm ◽  
Tissue Remodelling ◽  
Periodontal Health ◽  
Adverse Pregnancy ◽  
Crevicular Fluid ◽  
Microbial Change ◽  
The Relationship

Periodontitis is an inflammatory disease that affects the supporting structures of teeth. The presence of a bacterial biofilm initiates a destructive inflammatory process orchestrated by various inflammatory mediators, most notably proinflammatory cytokines, which are upregulated in the gingival crevicular fluid, leading to the formation of periodontal pockets. This represents a well-characterized microbial change during the transition from periodontal health to periodontitis; interestingly, the gestational condition increases the risk and severity of periodontal disease. Although the influence of periodontitis on pregnancy has been extensively reviewed, the relationship between pregnancy and the development/evolution of periodontitis has been little studied compared to the effect of periodontitis on adverse pregnancy outcomes. This review is aimed at summarizing the findings on the pregnancy-proinflammatory cytokine relationship and discussing its possible involvement in the development of periodontitis. We address (1) an overview of periodontal disease, (2) the immune response and possible involvement of proinflammatory cytokines in the development of periodontitis, (3) how bone tissue remodelling takes place with an emphasis on the involvement of the inflammatory response and metalloproteinases during periodontitis, and (4) the influence of hormonal profile during pregnancy on the development of periodontitis. Finally, we believe this review may be helpful for designing immunotherapies based on the stage of pregnancy to control the severity and pathology of periodontal disease.

scholarly journals Proinflammatory and Antimicrobial Nitric Oxide in Gingival Fluid of Diabetic Patients with Periodontal Disease

2006 ◽  
Vol 74 (12) ◽  
pp. 7010-7013 ◽  
Author(s):  
Uros Skaleric ◽  
Boris Gaspirc ◽  
Nancy McCartney-Francis ◽  
Andrej Masera ◽  
Sharon M. Wahl
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
No Synthase ◽  
Diabetic Patients ◽  
Prevotella Intermedia ◽  
Dual Roles ◽  
Crevicular Fluid ◽  
Gingival Fluid

ABSTRACT Abnormal nitric oxide (NO) synthesis has been implicated in the pathogenesis of both periodontal disease and diabetes mellitus. In diabetic patients, increased inducible NO synthase in inflamed gingiva correlated with NO in gingival crevicular fluid. Although increased NO reflected more-severe inflammation, it was associated with reductions in CFU of Prevotella intermedia, a major periodontopathogen, highlighting dual roles for NO.

Salivary Diagnosis - Clinical Uses in Assessing Oral Inflammation

2017 ◽  
Vol 68 (6) ◽  
pp. 1201-1204 ◽  
Author(s):  
Iulia Ioana Stanescu ◽  
Alexandra Totan ◽  
Florentina Rus ◽  
Daniela Miricescu ◽  
Brandusa Mocanu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Oxidative Stress Marker ◽  
Clinical Settings ◽  
Tissue Destruction ◽  
Whole Saliva ◽  
Oral Inflammation ◽  
Positive Correlations ◽  
Crevicular Fluid

The past decades demonstrated that saliva and its components represent a remarkable diagnosis fluid with valuable clinical uses for both oral and systemic diseases. At the same time it is well established that oxidative stress is involved in a wide number of pathologies, including periodontitis. The specific aim of the present study which included 50 subjects is to determine if saliva can be used in clinical settings to correlate oxidative stress and tissue destruction markers with the severity of periodontal disease. An important oxidative stress marker - 8-hydroxydesoxyguanosine (8-OHdG) and a collagen degradation marker - beta-crosslaps (b-CTX) were quantified in both saliva and gingival crevicular fluid (GCF) using ELISA kits and were found to be significantly increased in the chronic periodontitis group when compared to respective controls (p[0.05). At the same time positive correlations were observed between whole saliva and gingival crevicular fluid (p[0.05). Significant correlations were also determined between GCF and salivary markers and clinical parameters of periodontal disease. Present results demonstrate that saliva and its components can successfully be used in clinical settings and represents a reliable tool for assessing periodontal disease severity.

scholarly journals NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Petra Surlin ◽  
Luminita Lazar ◽  
Cerasella Sincar ◽  
Dorin Nicolae Gheorghe ◽  
Dora Maria Popescu ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Periodontal Disease ◽  
Chronic Hepatitis C ◽  
Inflammatory Reaction ◽  
Nlrp3 Inflammasome ◽  
Gingival Crevicular Fluid ◽  
Periodontal Status ◽  
Local Inflammatory Reaction ◽  
Crevicular Fluid

The study is aimed at assessing the impact that periodontal disease and chronic hepatitis C could have on gingival crevicular fluid levels of the NLRP3 inflammasome, caspase-1 (CASP-1), and interleukin-18 (IL-18) and at evaluating whether the increased local inflammatory reaction with clinical periodontal consequences is correlated to their upregulation. Patients were divided into four groups, according to their periodontal status and previously diagnosed hepatitis C, as follows: (i) CHC group, chronic hepatitis C patients; (ii) P group, periodontal disease patients, systemically healthy; (iii) CHC + P group, patients suffering from both conditions; and (iv) H group, systemically and periodontally healthy controls. Gingival crevicular samples were collected for quantitative analysis of the NLRP3 inflammasome, CASP-1, and IL-18. CHC + P patients expressed the worse periodontal status and the highest NLRP3, CASP-1, and IL-18 levels, the difference being statistically significant ( p < 0.05 ). The P group patients also expressed significantly more elevated NLRP3, CASP-1, and IL-18 levels, as compared to nonperiodontal patients (CHC and H groups). Chronic hepatitis C and periodontal disease could have a significant influence on the upregulation of NLRP3 inflammasome and its components, possibly contributing to an increased local inflammatory reaction and clinical periodontal consequences.

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