Symbiotic effect of lactobacillus acidophilus, Ginger, Pineapple and Green Coffee in the complex management of obesity in rats

2021 ◽  
Vol 30 (1) ◽  
pp. 143-152
Author(s):  
Aml Ghanem ◽  
Osama M. Abonama ◽  
Ahmed I. Abd El Maksoud ◽  
Mokhtar M. El-Zawahry ◽  
Dalia Elebeedy
Keyword(s):  
Body Fat ◽  
Lactobacillus Acidophilus ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Positive Control ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Green Coffee ◽  
Sprague Dawley

Background: Obesity is a chronic metabolic disease associated with having excess body fat that could be influenced by many factors. Our study aimed to assess the powerful effect of Lactobacillus acidophilus alone or combined (symbiotic) with Prebiotic such as Ginger, Pineapple and Green Coffee as anti-obesity agents. Methodology: Using 8 groups (10 rats each) of Sprague-Dawley rats, Group 1 was kept as a negative control, Group 2 positive control, while other groups were orally given Lactobacillus acidophilus, Ginger, Pineapple and Green Coffee individually and in combination with Probiotics, for 45 days till the end of the experiment while the body weight of rats was recorded.Blood samples were collected for biochemical parameters analysis and organs were dissected and homogenized to analyze obesity-related biomarkers, Results: Our results revealed that either individual or mixed administration of this pro and prebiotics decreased the body and organs, specifically those treated with the mixture or probiotic and prebiotic, also serum (HDL), CAT), and (SOD) was decreased (P <0.05), while other biochemical parameters (T.G), (CHOL), (U.A), (Creat), Urea, (GOT),(GPT) and (ALP); ( significantly (P<0.05) was decreased when compared with the positive control group, Nevertheless, the histopathological examination showed the reduction of adipose tissue in kidney, liver, and Pancreas showed overestimate reductions in the percentage of body fat. Conclusions: This study showed a promising effect of Lactobacillus acidophilus when it combined with these plants as natural feed additives on obesity.

scholarly journals Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 196 ◽  
Author(s):  
Hamed R. Takruri ◽  
Maha S. Shomaf ◽  
Saida F. Shnaigat
Keyword(s):  
Protective Effect ◽  
Mammary Cancer ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Light Cycle ◽  
Sprague Dawley ◽  
Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

scholarly journals Green coffee methanolic extract and silymarin protect against CCl4-induced hepatotoxicity in albino male rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haddad A. El Rabey ◽  
Samar M. Rezk ◽  
Mohamed I. Sakran ◽  
Ghena M. Mohammed ◽  
Omar Bahattab ◽  
...  
Keyword(s):  
Olive Oil ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Positive Control ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Green Coffee ◽  
Sprague Dawley

Abstract Background During the last few decades, patients worldwide have been interested in using alternative medicine in treating diseases to avoid the increased side effects of chemical medications. Green coffee is unroasted coffee seeds that have higher amounts of chlorogenic acid compared to roasted coffee. Green coffee was successfully used to protect against obesity, Alzheimer disease, high blood pressure and bacterial infection. Methods This study aimed to investigate the probable protective activity of the green coffee methanolic extract, silymarin and their combination on CCl4-induced liver toxicity in male rats. Thirty Sprague – Dawley male albino rats were divided into 5 groups; control negative (G1) just got the vehicle (olive oil) and the other four groups received CCl4 dissolved in olive oil through an intraperitoneal injection and were divided into untreated control positive group (G2), the third group (G3) was treated with green coffee methanolic extract, the fourth group (G4) was treated with silymarin, and the fifth group (G5) was treated with a combination of green coffee methanolic extract and silymarin. Results In the positive control group treated with CCl4 (G2), the CCl4-induced toxicity increased lipid peroxidation, IL-6, kidney function parameters, liver function enzymes, total cholesterol, triglycerides and low-density lipoproteins, and decreased irisin, antioxidants, CYP450 and high-density lipoprotein levels. Hepatic tissues were also injured. However, treating the injured rats in G3, G4 and G5 significantly improved the altered parameters and hepatic tissues. Conclusions Green coffee methanolic extract, silymarin, and their combination succeeded in protecting the male rats against CCl4 hepatotoxicity due to their antioxidant activity. Effect of green coffee methanolic extract mixed with silymarin in G5 was more efficient than that of green coffee methanolic extract in G3 or silymarin in G4.

scholarly journals Hematologic indices of the young rabbits with the preparation of the prebiotic preparation

2018 ◽  
Vol 20 (84) ◽  
Author(s):  
O. B. Tsyganchuk
Keyword(s):  
Biochemical Parameters ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Intercellular Spaces ◽  
Biochemical Processes ◽  
The Third ◽  
Economic Experience ◽  
Main Period ◽  
Effect Of Feeding

Blood is a liquid tissue that carries in the body the transport of chemicals, thanks to which the integration of biochemical processes takes place that take place in various cells and intercellular spaces, into a single system. The purpose of the study was to investigate the effect of feeding various doses of  Prebiolact-Kr on the morphological and biochemical parameters of the blood of repairing young rabbits. One of the new feed additives with prebiotic action is Prebiolakt-Kr, which was created by employees of the scientific and biotechnological enterprise «BTU Center» in Ladyzhin, Vinnytsia region. Studies were carried out on four groups of young rabbits, selected for the principle of analogs of 25 heads in each. The material for the scientific and economic experience was the rabbit of the modern meat hybrid hyplus. The comparative period lasted for seven days. The duration of the main period of the experiment is 126 days. The animals of the second group received Prebiolact-Kr in the amount of 1.5 g for the full-scale granulated mixed fodder, the third for 2.0 grams and the fourth for 2.5 grams per head per day. Blood for research was selected for serological plugs by puncturing the terminal ear vein with an injection needle. When taking blood, the rules of asepsis and antiseptics were observed. The picture of the state of blood is a symptomatic reflection of changes in the intensity of all metabolic processes that occur in the body of animals under the influence of certain food factors. As studies have shown, the number of uniform elements is within the limits of the norm, at the level of the control group. However, those deviations that were observed in the experiment were unbelievable. The positive result is that the administration of the drug did not affect the content of macro- and microelements in the blood, so it can be concluded that the administration of Prebiolact-Kr in the diet of experimental animals did not cause changes in the morphological and biochemical parameters of the blood.

scholarly journals Evaluation of detoxifying activity of ORGCHP against acetaminophen induced hepatotoxicity in Sprague Dawley rats

2020 ◽  
Vol 9 (6) ◽  
pp. 912
Author(s):  
Subbu KesavaRaja Vasudevan ◽  
Suresh Seetharam ◽  
Anbudhasan Poongavanam
Keyword(s):  
Chlorella Vulgaris ◽  
Methyl Cellulose ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
The Body ◽  
Control Group ◽  
High Dose ◽  
Test Drug ◽  
Sprague Dawley ◽  
Pathological Evaluation

Background: Accumulation of toxins in the body over a period of time interferes with the normal body functioning. The removal of toxins from the body is called ‘detoxification’. The liver is the main organ involved in detoxification and damage to the liver may impede the removal of toxins. The present study assessed the hepatoprotective activity of an organic Chlorella vulgaris in acetaminophen-induced liver damage model.Methods: A total of 35 animals were randomized to five groups with seven animals in each group. The test drug, organic Chlorella vulgaris (ORGCHP; 350 mg/kg and 700 mg/kg) was compared with a reference standard (200 mg/kg). The positive control group and the vehicle control group were administered 0.5% w/v carboxy methyl cellulose. All groups received dose volume of 10 ml/kg for 10 days. Acetaminophen was given on day 8 and day 9. Blood collections were done at baseline day 1, day 8 and day 10. Outcome measures were the change in body weight, oxidation biomarkers, histopathological evaluation, gross pathological evaluation and relative body weight.Results: Test drug ORGCHP Chlorella vulgaris showed dose dependent reduction in hepatoxicity with high reduction in aspartate transaminase (AST) and modest lowering of alanine transaminase (ALT) (350 mg/kg; p.o.); while at high dose (700 mg/kg; p.o.) there was significant reduction in alkaline phosphatase (p<0.05), ALT (p<0.001) and AST (p<0.001) levels. No pathological or histopathological abnormality was seen in control group. In drug group, one animal showed minimal necrosis, while mild and moderate necrosis was seen in three animals each respectively.Conclusions: The test drug exhibits detoxifying and hepatoprotective activity against acetaminophen induced hepatotoxicity.

Local mineral feed additives in the rations of steers of Hereford breed

2020 ◽  
pp. 28-39
Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya
Keyword(s):  
Economic Effect ◽  
Live Weight ◽  
Negative Influence ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Mineral Salts ◽  
Meat Productivity ◽  
Experimental Group ◽  
Mineral Feed Additives

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.

The effectiveness of use of enterosorbent Shelltic Es when rearing of young pigs

2020 ◽  
pp. 3-11
Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Internal Organs ◽  
Feed Digestibility ◽  
Young Pigs ◽  
Carcinogenic Effects ◽  
Experimental Group

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.

The Effect of Feeding Date Palm Fruit (Phoeix dactylifera L.) on Energy, Macronutrient, and Fiber Intakes of a Convenient Sample of Females

2020 ◽  
Vol 16 (8) ◽  
pp. 1270-1277
Author(s):  
Hiba F. Al-Sayyed ◽  
Hamed R. Takruri ◽  
Nawal A. Bakir ◽  
Dima H. Takruri
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Date Palm ◽  
The Body ◽  
Control Group ◽  
Hormone Concentration ◽  
Palm Fruit ◽  
Menstrual Status ◽  
Feeding Duration ◽  
Effect Of Feeding

Background : Female menstrual changes in hormone concentration influence appetite and eating behavior. As well, eating pattern has some influences on menstrual hormonal status. Feeding date palm fruit has been found in our previous report to affect menstrual hormones and some menstrual parameters. Objective: The aim of this research is to study the effect of feeding dates on energy, macronutrient, and fiber intakes, body weight, and body fat%. Additionally, this study aims to find any correlation between menstrual hormone concentration and the above-mentioned parameters. Methods: A convenient sample (n=37) of not-sexually active females aged 20-30 were divided into two groups; one group (dates group) was fed 7 dates and the other served as a control group. The participants of the control group were exposed to the same experimental conditions except for eating dates. Throughout the feeding trial, the volunteers filled a 3-day food record; one menses day and two non-menses days. Results: There was a significant effect of the menstrual status-feeding duration interaction in terms of protein and fat intakes. Additionally, menstrual status, as well as feeding duration, affected energy and macronutrient intakes significantly. Furthermore, feeding duration affected fiber intake significantly. Despite the above-mentioned differences in energy and macronutrient intakes, these effects didn’t affect the body weight and body mass index of the study participants, neither their body fat%. Moreover, we have found significant correlations between nutrient intakes and menstrual hormone concentration. Conclusion : Feeding dates affected energy, macronutrient, and fiber intakes significantly. These effects were related to the changes in menstrual hormone concentration.

Acute toxicity of Potentilla anserina L. extract in mice

2020 ◽  
Vol 75 (5-6) ◽  
pp. 129-134 ◽  
Author(s):  
Dul Dram ◽  
Cui-Zhu Zhao ◽  
Qin-Ge Ma ◽  
Jun-Wei He ◽  
Jia-Jie Duo ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Maximum Dose ◽  
Histopathological Examination ◽  
Oral Dosage ◽  
Control Group ◽  
Acute Toxicity Study ◽  
Organ Index ◽  
Potentilla Anserina ◽  
Safety Range

AbstractPotentilla anserina L. is not only a medicinal plant, but also a traditional cuisine. Hence, an acute toxicity study was performed to confirm its safety profile. Forty Kunming mice were randomly divided into two groups: control group and P. anserina L. extract group. Using the maximum dosage method, the P. anserina L. extract group was given the maximum dose within 12 h, equivalent to 345.6 g/kg crude drug. The control group was given distilled water. After administration, toxicity symptoms of mice were observed, body weight and food intake were recorded. After 14 days, blood was collected to measure biochemical parameters, autopsy was carried out to observe the changes of organs, and the vital organs were separated, weighed, and preserved for histopathological examination. The results showed that P. anserina L. extract group had no toxic symptoms. The activity, weight, and diet of mice were normal, and no abnormality was found in organ index, renal function, liver function, anatomical observation, and histopathological examination. Therefore, the maximum oral dosage (345.6 g/kg) of P. anserina L. was good safety. This study indicated that P. anserina L. had a large safety range and the clinical application was safe.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals Electroacupuncture Reduces Weight in Diet-Induced Obese Rats via Hypothalamic Tsc1 Promoter Demethylation and Inhibition of the Activity of mTORC1 Signaling Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Jincheng Leng ◽  
Feng Xiong ◽  
Junpeng Yao ◽  
Xiahuan Dai ◽  
Yulei Luo ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Body Fat ◽  
Mammalian Target Of Rapamycin ◽  
Gene Promoter ◽  
Chow Group ◽  
Diet Group ◽  
The Body ◽  
Sprague Dawley ◽  
Tsc1 Gene ◽  
Mtorc1 Signaling

Subject. The study aimed to investigate the mechanism of electroacupuncture reducing weight via tuberous sclerosis complex 1 (Tsc1) promoter methylation, inhibiting the mammalian target of rapamycin complex 1 (mTORC1) pathway. Materials and Methods. Male Sprague-Dawley rats were divided into chow-fed group (chow group) or high-fat diet group (HF group) for 14 weeks. The obesity rats in HF group were randomly divided into electroacupuncture group (EA group) and diet-induced obesity (DIO) group, which received EA stimulation on bilateral ST25, RN12, SP6, and ST36 for 4 weeks or no further treatment, respectively. Methylation of the Tsc1 gene promoter and expression of agouti-related protein (AgRP), neuropeptide Y (NPY), and proopiomelanocortin (PoMC) were detected at the 18th week. Results. At week 18, weight, body fat, and the body fat rate in DIO group were significantly higher than those of the chow and EA group. Compared with the chow group, the DIO group had increased methylation of the Tsc1 gene promoter and expression of mTORC1, AgRP, and NPY gene and decreased PoMC in the hypothalamus; after EA, methylation of the Tsc1 gene promoter, mRNA, and protein of the mTORC1 and expression of AgRP and NPY gene decreased and PoMC increased significantly. Conclusions. Our study could shed light on the potential pathway where EA exerts effects on the mechanism of EA treatment for obesity through the hypothalamic Tsc1 promoter demethylation and inhibition of the activity of mTORC1 signaling pathway.

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