Comparison between Specific Immunoglobulin E and Skin Prick Test for Diagnosis of Allergic Patients

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 173-179
Author(s):  
Wafaa S. Metwally ◽  
Ghada A. Mokhtar
Keyword(s):  
Skin Prick Test ◽  
Immunoglobulin E ◽  
Diagnostic Yield ◽  
Good Alternative ◽  
Specific Ige ◽  
Gold Standard Method ◽  
Serum Ige ◽  
Prick Test ◽  
Serum Total Ige

Background: Skin prick test (SPT) has been identified as the gold standard method to diagnose IgE mediated hypersensitivities; it is accurate, easy and cheap. However, SPT has some important limitations, so in vitro serum specific serum IgE (sSIgE) detection can be a good alternative. The diagnostic yield of sSIgE testing usually depends on the specific allergen. Objective: This study compares between SPT and sSIgE for diagnosis of allergy. Methodology: 61 allergic patients enrolled from Zagazig University Allergy and Immunology Unit, tested by SPT for the common allergens. Serum total IgE and specific IgE levels were measured and compared to SPT results. Results: The over- all sensitivities of sSIgE were excellent (75-100%) for most of allergens tested. PPV was excellent with some allergens (pollens, cockroaches, mites) and poor with others (cat hair). Sensitivity of mites sSIgE was excellent with PPV approaching 90%, specificiy was only 13%. However, the NPV was 100%. Significant level of agreement and correlation between the results of SPT and sSIgE for cockroaches and molds was reported. Positive correlation between the total levels of serum IgE and the diameter of wheal and flare of the SPT for mites, pollens and cockroaches was found. Conclusion: The sensitivities of sSIgE were excellent for most of allergens tested. Specificity of sSIgE varies between individual allergens. Serum specific IgE testing may be a good alternative to skin prick test if the latter could not be carried out.

scholarly journals Concordance of Skin Prick Test, Intradermal Testing, Serum IgE Levels and Symptoms in Patients with Allergic Rhinitis

Allergies ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 181-194
Author(s):  
Tilman Huppertz ◽  
Martha Dahlem ◽  
Veronika Weyer-Elberich ◽  
Boris R. Haxel
Keyword(s):  
Allergic Rhinitis ◽  
Skin Prick Test ◽  
Specific Ige ◽  
Screening Tools ◽  
Study Cohort ◽  
Intradermal Testing ◽  
Serum Ige ◽  
Prick Test ◽  
Specific Serum ◽  
Symptom Patterns

Background: Diagnosis of allergic rhinitis is achieved by a combination of patient history and different screening tools, followed by specific provocation testing. Screening tools usually involve a skin prick test (SPT), specific serum IgE or a combination of both. Objective: The purpose of this study was to evaluate the correlation of SPT, intradermal testing and specific serum IgE testing in certain allergens and to evaluate sensitization rates, symptom patterns and time of symptoms in a cohort of patients with suspected allergic rhinitis. Methods: Data on 4653 patients with suspected allergic rhinitis were included and divided into five groups: spring bloomers (birch, hazel, etc.), summer bloomers (grasses and rye), autumn bloomers (ribwort and mugwort), mites and mold. Correlation of SPT, intradermal testing and specific IgE test results using Cohen’s kappa and logistic regression were carried out to evaluate the probability of symptoms. Results: Comparison of SPT and specific serum IgE led to kappa coefficients between 0.33 and 0.47, corresponding to a minor to moderate concordance. Comparing the symptoms reported by patients with sensitization diagnosed by SPT, a correlation was only found for spring and summer bloomers with an odds ratio of 1.5 and 2.1, respectively. The most prevalent symptom in the study cohort was rhinitis, followed by others such as asthma, sense of smell and atopic dermatitis. Conclusions: SPT seems to be more sensitive than specific IgE for detection of sensitization. Patients’ symptoms as well as the timing of symptoms, especially for perennial allergies, are not always very pronounced.

Alatop: A New In Vitro Screening Test for Atopy

1994 ◽  
Vol 22 (6) ◽  
pp. 313-322
Author(s):  
M Yazicioǧlu ◽  
P Sayinbaş ◽  
Ü Öneş ◽  
A Saltik ◽  
M Tug˜rul
Keyword(s):  
Antibody Test ◽  
Atopic Disease ◽  
Specific Ige ◽  
Total Serum ◽  
Healthy Children ◽  
Serum Ige ◽  
Prick Test ◽  
Skin Prick Tests ◽  
Inhalant Allergens

The value of a new in vitro multiallergen IgE antibody test in the diagnosis of atopy in children was compared with that of skin-prick tests and total serum IgE level determination. Twenty children with clinical histories and symptoms of asthma, median age 5 years, and 20 healthy children, median age 5.5 years, were enrolled. Total serum IgE levels were evaluated as either normal or high by referring to kit values. Specific IgE antibodies to 12 different inhalant allergens were screened by the Alatop test. The accuracy figures for the tests compared with clinical diagnosis were 65.0%, 72.5% and 80.0%, for the total serum IgE determination, the Alatop test and the skin-prick test, respectively, and the other measures of clinical reliability showed a similar pattern. Although the skin-prick tests were the most sensitive and specific of the three, for screening atopic disease in children, the Alatop test provides a valuable alternative, and the combined use of skin-prick tests with the Alatop test will provide a more reliable screen than using any single test.

scholarly journals Suitable Interpretation of Skin Prick Test and Biomedical Guidance Leads to a Better Clinical State in Atopic Individuals with High Indoor Permanence: Possible Therapeutic Implications

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 222-230
Author(s):  
Bernardo Yassunobu Nakamatsu ◽  
Archangelo Padreca Fernandes ◽  
Débora da Silva ◽  
Ludimila Souza Santos ◽  
Thamires Rodrigues de Sousa ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Clinical Symptoms ◽  
Specific Ige ◽  
Substantial Improvement ◽  
Clinical State ◽  
Prick Test ◽  
Therapeutic Implications ◽  
Social Habits ◽  
Indoor Conditions

Indoor conditions contribute to allergen sensitization and multiple allergens reactivity, mainly for inhaled allergens. This study analyzes if Skin Prick Test (SPT) combined with efficient individual biomedical guidance about allergy development’s social, biological, and environmental aspects can yield a better clinical state with therapeutic implications for atopic individuals with high indoor permanence. We recruited atopic and non-atopic volunteers (clinically and in vitro diagnosed) with indoor permanence above 15 h per day and without previous SPT evaluation. The SPT and serum anti-allergen IgE analyses were performed individually in person, demonstrated, and discussed by the practitioners. Six months after, SPT and specific IgE titers determination were repeated, and a questionnaire to evaluate the effectiveness of the practitioner’s orientation was performed. After six months, 14% of atopic volunteers reported changes in their social habits, 30% said that they avoid the development of allergies clinical symptoms, and 68% reported a substantial improvement in their health after being informed mentored about their allergen reactivity. The control non-atopic group, as expected, reported no changes in social habits, the maintenance of total avoidance of allergic symptoms, and almost no improvement of their health. Reduced SPT and serum allergen-specific IgE titers were detected in the atopic individuals corroborating with questionnaire results. Our results indicated that SPT, followed by an individual and efficient discussion about the main biomedical aspects of allergy development, could exert a pronounced therapeutic role in allergy development by high indoor permanence individuals.

Measurement of natural rubber latex allergen levels in medical gloves by allergen-specific IgE-ELISA inhibition, RAST inhibition, and skin prick test

Allergy ◽  
1998 ◽  
Vol 53 (1) ◽  
pp. 59-67 ◽  
Author(s):  
T. Palosuo ◽  
S. Mäkinen-Kiljunen ◽  
H. Alenius ◽  
T. Reunala ◽  
E. Yip ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Skin Prick Test ◽  
Natural Rubber Latex ◽  
Specific Ige ◽  
Rubber Latex ◽  
Prick Test ◽  
Elisa Inhibition

scholarly journals Study of causal aeroallergens in allergic rhinitis

2019 ◽  
Vol 5 (4) ◽  
pp. 916
Author(s):  
Divya Aggarwal ◽  
Abhilash S. ◽  
Sunil Kapur ◽  
Divya Gupta
Keyword(s):  
Allergic Rhinitis ◽  
Skin Prick Test ◽  
Symptom Score ◽  
Eosinophil Count ◽  
Severe Disease ◽  
Nasal Symptom ◽  
Total Serum ◽  
Serum Ige ◽  
Prick Test ◽  
Absolute Eosinophil Count

<p class="abstract"><strong>Background:</strong> The diagnosis of allergic rhinitis requires a detailed history, thorough clinical examination and identification of aeroallergens by skin prick test. The diagnosis cannot be confirmed on the basis of symptoms alone because both allergic and non-allergic rhinitis can present with similar symptoms. The present study was undertaken to identify the common aeroallergens causing allergic rhinitis and to find any relationship of the severity of patient’s symptoms with raised levels of absolute eosinophil count, percentage of eosinophils in the peripheral blood and total serum IgE.</p><p class="abstract"><strong>Methods:</strong> It is cross sectional study was conducted among 100 patients including both the genders attending out-patient department of ENT. The patients were divided into mild, moderate and severe disease categories based on nasal symptom score (NSS) which was calculated taking into account the severity of four symptoms namely sneezing, watery rhinorrhoea, itching sensation in nose and nasal obstruction.  </p><p class="abstract"><strong>Results:</strong> 76% of patients had moderate nasal symptom score and 42% had symptoms of allergic rhinitis for less than 3 years. The prevalence of skin prick test was strongly positive for pollens (46.19%), followed by dust (16.4%), dust mites (15.7%), fungus (9.7%), insects (9.24%) and epithelia (2.77%). But, among 68 aeroallergens, most common offending allergen was D-farinae (30%). There was no significant relationship found to exist between Eosinophilia, Absolute eosinophil count and serum IgE level with that of nasal symptom score.</p><p class="abstract"><strong>Conclusions:</strong> Skin prick test is the gold standard investigation of choice for definitive diagnosis of allergic rhinitis.</p><p class="abstract"> </p>

A comparison of specific IgE and skin prick test results to common environmental allergens using the HYTEC™ 288

2018 ◽  
Vol 462 ◽  
pp. 9-12 ◽  
Author(s):  
Vijaya Knight ◽  
Molly L. Wolf ◽  
Anita Trikha ◽  
Douglas Curran-Everett ◽  
Melissa Hiserote ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Specific Ige ◽  
Test Results ◽  
Prick Test
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