Evaluation of the Antimicrobial Activity of Citric Acid Functionalized Magnetite Nanoparticles

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 143-149
Author(s):  
Ahmed M. El-Khawaga ◽  
Ayman A. Farrag ◽  
Ahmed I. El-Batal ◽  
Mohamed A. Elsayed
Keyword(s):  
Antimicrobial Activity ◽  
Citric Acid ◽  
Magnetite Nanoparticles ◽  
Pathogenic Bacteria ◽  
Average Particle Size ◽  
Maximum Activity ◽  
Precipitation Method ◽  
Average Particle ◽  
Effective Prevention ◽  
Fe3o4 Nps

Background: Antimicrobial resistance (AMR) has emerged as one of the principal public health problems of the 21st century that threatens the effective prevention and treatment of an ever-increasing range of infections caused by bacteria no longer susceptible to the common medicines used to treat them. Objectives: To development of Fe3O4 NPs with specific sizes and shapes Potential as a new antibacterial agent. Methodology: In this study magnetite nanoparticles (Fe3O4 NPs) were synthesized through an aqueous co-precipitation method and functionalized with citric acid for outstanding their antimicrobial potential. Fe3O4 NPs were characterized by XRD, TEM, SEM, EDX and FTIR to analyze crystallinity, average particle size, morphology and functional groups, respectively. Antimicrobial activity was investigated against pathogenic bacteria as zone of inhibition (ZOI) and minimum inhibitory concentration (MIC). Results: Antimicrobial results showed that CA- Fe3O4 NPs owns maximum activity against Staphylococcus aureus and E. coli by 18.0 and 15.0mm ZOI, respectively. Conclusion: It should be noted that (CA-Fe3O4) NPs are also active upon Gram-positive than Gram- negative bacteria. The synthesized (CA-Fe3O4) NPs are promising for potential applications as antimicrobial agent and in drug delivery fields.

scholarly journals Synthesis of ZnO Nanoparticles by Precipitation Method with Their Antibacterial Effect

2018 ◽  
Vol 16 (2) ◽  
pp. 117 ◽  
Author(s):  
Muhammad Fajri Romadhan ◽  
Nurgaha Edhi Suyatma ◽  
Fahim Muchammad Taqi
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Average Particle Size ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Zinc Nitrate ◽  
Sem Analysis ◽  
Precipitation Method ◽  
Average Particle ◽  
Zno Nps

The aim of this study was to synthesize and characterize Zinc oxide nanoparticles (ZnO-NPs) prepared by precipitation method. Zinc nitrate and sodium hydroxide was used as starting materials with biopolymer pectin as capping agent. ZnO-NPs were synthesized at three levels of temperatures (60, 80 and 100 °C) without or with calcinations (500 °C). Particle size analyzer (PSA) analysis results showed that the samples without calcination (T60, T80 and T100) having an average particle size respectively 105.13, 78.53, and 76.43 nm, whereas at the samples by calcination (T60C, T80C and T100C) each have average particle size of 88.73, 44.30 and 543.77 nm. The results showed that preparation of ZnO-NPs by using heating at 80 °C followed with calcinations at 500 °C (T80C) produced the smallest size. T80C samples further were analyzed using XRD, SEM and the antimicrobial activity compared with the ZnO-NPs commercials. XRD analysis confirmed that ZnO-NPs were successfully obtained and have form of pure nanostructure. SEM analysis showed that ZnO-NPs obtained has a spherical shape. Furthermore, this ZnO-NPs (T80C) has a better antimicrobial activity compared than commercial ZnO-NPs in market.

scholarly journals Novel Cubic Magnetite Nanoparticle Synthesis Using Room Temperature Ionic Liquid

2012 ◽  
Vol 9 (3) ◽  
pp. 1070-1076 ◽  
Author(s):  
M. Sundrarajan ◽  
M. Ramalakshmi
Keyword(s):  
Electron Microscopy ◽  
Ionic Liquid ◽  
Iron Oxide ◽  
Magnetite Nanoparticles ◽  
Room Temperature ◽  
Average Particle Size ◽  
Precipitation Method ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Ft Ir

Room Temperature Ionic liquids are relatively more useful in the synthesis of inorganic nanostructured materials because of their unique properties. To synthesize the iron oxide nanoparticle in simple precipitation method, a novel ionic liquid was used as the greener medium and stabilizing agent namely “1-n-butyl-3-methylimidazolium trifluoromethane sulfonate [BMIM][TfO]”. The crystallinity, chemical structure, morphology and magnetic properties of the synthesized magnetite nanoparticles have been characterized by using X-ray diffraction (XRD), Fourier Transform Infrared (FT-IR), Scanning electron microscopy (SEM), Atomic force microscopy(AFM), Transmission electron microscopy (TEM) and Vibrating sample magnetometer (VSM) studies. The XRD study is divulge that the synthesized magnetite nanoparticles have inverse spinel face centered cubic structure. The FT-IR vibration peaks show the formation of Fe3O4nanoparticles, where the vibration peak for Fe-O is deliberately presence at 584 cm-1. The average particle size of the synthesized nanoparticles is found to be 35 nm. Homogeneously dispersed cubic shape with superstructure is found through SEM, AFM and TEM examination studies. The synthesized iron oxide nanoparticles have a high saturation magnetization value of 25 emu/g, which is very much useful for biomedical applications.

Enhanced oral bioavailability of nisoldipine-piperine-loaded poly-lactic-co-glycolic acid nanoparticles

2017 ◽  
Vol 6 (6) ◽  
pp. 517-526 ◽  
Author(s):  
Permender Rathee ◽  
Anjoo Kamboj ◽  
Shabir Sidhu
Keyword(s):  
Oral Bioavailability ◽  
Drug Loading ◽  
Average Particle Size ◽  
Entrapment Efficiency ◽  
Pharmacokinetic Interaction ◽  
Precipitation Method ◽  
Pharmacokinetic Studies ◽  
Average Particle

AbstractBackground:Piperine helps in the improvement of bioavailability through pharmacokinetic interaction by modulating metabolism when administered with other drugs. Nisoldipine is a substrate for cytochrome P4503A4 enzymes. The study was undertaken to assess the influence of piperine on the pharmacokinetics and pharmacodynamics of nisoldipine nanoparticles in rats.Methods:Optimization studies of nanoparticles were performed using Taguchi L9 orthogonal array, and the nanoparticles were formulated by the precipitation method. The influence of piperine and nanoparticles was evaluated by means of in vivo kinetic and dynamic studies by oral administration in rats.Results:The entrapment efficiency, drug loading, ζ potential, and average particle size of optimized nisoldipine-piperine nanoparticles was 89.77±1.06%, 13.6±0.56%, −26.5 mV, and 132±7.21 nm, respectively. The in vitro release in 0.1 n HCl and 6.8 pH phosphate buffer was 96.9±0.48% and 98.3±0.26%, respectively. Pharmacokinetic studies showed a 4.9-fold increase in oral bioavailability and a >28.376±1.32% reduction in systemic blood pressure by using nanoparticles as compared to control (nisoldipine suspension) in Wistar rats.Conclusion:The results revealed that piperine being an inhibitor of cytochrome P4503A4 enzymes enhanced the bioavailability of nisoldipine by 4.9-fold in nanoparticles.

scholarly journals Preparation and characterization of domperidone nanoparticles for dissolution improvement

2018 ◽  
Vol 27 (1) ◽  
pp. 39-52
Author(s):  
Mohammed Sabar Al-lami ◽  
Malath H. Oudah ◽  
Firas A. Rahi
Keyword(s):  
Particle Size ◽  
Average Particle Size ◽  
In Vitro Release ◽  
Methyl Cellulose ◽  
Precipitation Method ◽  
Average Particle ◽  
Antisolvent Precipitation ◽  
Stirring Time

This study was carried out to prepare and characterize domperidone nanoparticles to enhance solubility and the release rate. Domperidone is practically insoluble in water and has low and an erratic bioavailability range from 13%-17%. The domperidone nanoparticles were prepared by solvent/antisolvent precipitation method at different polymer:drug ratios of 1:1 and 2:1 using different polymers and grades of poly vinyl pyrolidone, hydroxy propyl methyl cellulose and sodium carboxymethyl cellulose as stabilizers. The effect of polymer type, ratio of polymer:drug, solvent:antisolvent ratio, stirring rate and stirring time on the particle size, were investigated and found to have a significant (p? 0.05) effect on particle size. The best formula was obtained with lowest average particle size of 84.05. This formula was studied for compatibility by FTIR and DSC, surface morphology by FESEM and crystalline state by XRPD. Then domperidone nanoparticles were formulated into a simple capsule dosage form in order to study of the in vitro release of drug from nanoparticles in comparison raw drug and mixture of polymer:drug ratios of 2:1. The release of domperidone from best formula was highly improved with a significant (p? 0.05) increase.

scholarly journals Effect of the polymeric coating over Fe3O4 particles used for magnetic separation

2010 ◽  
Vol 8 (5) ◽  
pp. 1041-1046 ◽  
Author(s):  
Raúl Reza ◽  
Carlos Martínez Pérez ◽  
Claudia Rodríguez González ◽  
Humberto Romero ◽  
Perla García Casillas
Keyword(s):  
Particle Size ◽  
Magnetite Nanoparticles ◽  
Average Particle Size ◽  
Spherical Shape ◽  
Polymeric Coating ◽  
Average Particle ◽  
Synthesis Conditions ◽  
Aging Conditions ◽  
Magnetite Particles ◽  
Average Size

AbstractIn this work, the synthesis of magnetite nanoparticles by two variant chemical coprecipitation methods that involve reflux and aging conditions was investigated. The influence of the synthesis conditions on particle size, morphology, magnetic properties and protein adsorption were studied. The synthesized magnetite nanoparticles showed a spherical shape with an average particle size directly influenced by the synthesis technique. Particles of average size 27 nm and 200 nm were obtained. When the coprecipitation method was used without reflux and aging, the smallest particles were obtained. Magnetite nanoparticles obtained from both methods exhibited a superparamagnetic behavior and their saturation magnetization was particle size dependent. Values of 67 and 78 emu g−1 were obtained for the 27 nm and 200 nm magnetite particles, respectively. The nanoparticles were coated with silica, aminosilane, and silica-aminosilane shell. The influence of the coating on protein absorption was studied using Bovine Serum Albumin (BSA) protein.

Development of propolis nanoparticles for the treatment of bovine mastitis: in vitro studies on antimicrobial and cytotoxic activities

2019 ◽  
Vol 99 (4) ◽  
pp. 713-723 ◽  
Author(s):  
Gabriela Tasso Pinheiro Machado ◽  
Maria Beatriz Veleirinho ◽  
Letícia Mazzarino ◽  
Luiz Carlos Pinheiro Machado Filho ◽  
Marcelo Maraschin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Encapsulation Efficiency ◽  
Average Particle Size ◽  
Physical Stability ◽  
Bovine Mastitis ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Average Particle ◽  
Propolis Extract

This study describes the development of propolis nanoparticles (PNP) to treat bovine mastitis. Three PNP prepared with varying concentrations of propolis (5% and 7%, w/v) and the surfactants [poloxamer (1%, 3%, and 4%, w/v) and soy lecithin (0.25%, 0.7%, and 1%, w/v)]. PNP were characterized according to their size, polydispersity, zeta potential, pH, morphology, and physical stability. PNP were evaluated for their in vitro antimicrobial and cytotoxic effects. PNP obtained were spherical with a monodisperse distribution (polydispersity index < 0.2) and an average particle size between 181 and 201 nm. Stability studies showed that PNP were stable over 150 d. The encapsulation efficiency of total phenolic content varied between 73% and 91%. The chromatographic profile of phenolic compounds from PNP showed selective encapsulation efficiency according to the polarity of compounds. All PNP showed antimicrobial activity against Staphylococcus aureus with a minimum inhibitory concentration ranging from 156 to 310 μg mL−1. The IC50 (the concentration responsible for reduction of cellular viability by half) for epithelial cells of bovine mammary gland (MAC-T, mammary alveolar cell-T) varied from 122.2 to 268.4 μg mL−1. Results showed that PNP represent a promising nanocarrier for high concentrations of propolis extract in a stable aqueous medium, while, at the same time, presenting antimicrobial activity accompanied by moderate cytotoxicity to the MAC-T cells.

Morphology-Controlled CaCO3 Nanostructures by Modified Co-Precipitation in Pulsed Mode

2015 ◽  
Vol 752-753 ◽  
pp. 148-153
Author(s):  
M.M. Nassar ◽  
Taha Ebrahiem Farrag ◽  
M.S. Mahmoud ◽  
Sayed Abdelmonem
Keyword(s):  
Particle Size ◽  
Calcium Carbonate ◽  
Rotation Speed ◽  
Average Particle Size ◽  
Calcium Nitrate ◽  
Precipitation Method ◽  
Average Particle ◽  
X Ray ◽  
Co Precipitation ◽  
Pulsed Mixing

Calcium carbonate nanoparticles and nanorods were synthesized by precipitation from saturated sodium carbonate and calcium nitrate aqueous solutions through co precipitation method. A new rout of synthesis was done by both using pulsed mixing method and controlling the addition of calcium nitrate. The effect of the agitation speed, and the temperature on particle size and morphology were investigated. Particles were characterized using X-ray Microanalysis, X-ray analysis (XRD) and scanning electron microscopy (SEM). The results indicated that increasing the mixer rotation speed from 3425 to 15900 (rpm) decreases the average particle size to 64±7 nm. A rapid nucleation then aggregation induced by excessive shear force phenomena could explain this observation. Moreover, by increasing the reaction temperature, the products were converted from nanoparticle to nanorods. The maximum attainable aspect ratio was 6.23 at temperature of 75°C and rotation speed of 3425. Generally, temperature raise promoted a significant homoepitaxial growth in one direction toward the formation of calcite nanorods. Overall, this study can open new avenues to control the morphology of the calcium carbonate nanostructures.

scholarly journals Facile Preparation of CNT/Ag2S Nanocomposites with Improved Visible and NIR Light Photocatalytic Degradation Activity and Their Catalytic Mechanism

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 503 ◽  
Author(s):  
Lijing Di ◽  
Tao Xian ◽  
Xiaofeng Sun ◽  
Hongqin Li ◽  
Yongjie Zhou ◽  
...  
Keyword(s):  
Catalytic Mechanism ◽  
Dye Degradation ◽  
Average Particle Size ◽  
Hybrid Nanocomposites ◽  
Light Irradiation ◽  
Precipitation Method ◽  
Average Particle ◽  
Nir Light ◽  
Photocatalytic Activities ◽  
Ag2s Nanoparticles

In this work, a series of carbon nanotubes (CNT)/Ag2S hybrid nanocomposites were successfully prepared by a facile precipitation method. Transmission electron microscope (TEM) observation indicates that Ag2S nanoparticles with an average particle size of ~25 nm are uniformly anchored on the surface of CNT. The photocatalytic activities of the CNT/Ag2S nanocomposites were investigated toward the degradation of rhodamine B (RhB) under visible and near-infrared (NIR) light irradiation. It is shown that the nanocomposites exhibit obviously enhanced visible and NIR light photocatalytic activities compared with bare Ag2S nanoparticles. Moreover, the recycling photocatalytic experiment demonstrates that the CNT/Ag2S nanocomposites possess excellent photocatalytic stability. The photoelectrochemical and photoluminescence measurements reveal the efficient separation of photogenerated charges in the CNT/Ag2S nanocomposites. This is the dominant reason behind the improvement of the photocatalytic activity. Based on active species trapping experiments, the possible photocatalytic mechanism of CNT/Ag2S nanocomposites for dye degradation under visible and NIR light irradiation was proposed.

scholarly journals High Yield Synthesis and Application of Magnetite Nanoparticles (Fe3O4)

2020 ◽  
Vol 6 (2) ◽  
pp. 22 ◽  
Author(s):  
Charles Wroblewski ◽  
Tunde Volford ◽  
Blake Martos ◽  
Jurek Samoluk ◽  
Perry Martos
Keyword(s):  
Magnetite Nanoparticles ◽  
De Novo ◽  
Average Particle Size ◽  
Total Iron ◽  
High Yield ◽  
Chemical Compositions ◽  
Average Particle ◽  
Iron Salt ◽  
Mechanical Agitation ◽  
Aqueous Synthesis

Magnetite nanoparticles (Fe3O4), average particle size of 12.9 nm, were synthesized de novo from ferrous and ferric iron salt solutions (total iron salt concentration of 3.8 mM) using steady-state headspace NH3(g), 3.3% v/v, at room temperature and pressure, without mechanical agitation, resulting in >99.9% yield. Nanoparticles size distributions were based on enumeration of TEM images and chemical compositions analyzed by: XRD, EDXRF, and FT-IR; super-paramagnetic properties were analyzed by magnetization saturation (74 emu/g). Studies included varying headspace [NH3(g)] (1.6, 3.3, 8.4% v/v), and total iron concentrations (1.0 mM, 3.8 mM, 10.0 mM, and >>10 mM). An application of the unmodified synthesized magnetite nanoparticles included analyses of tetracycline’s (50, 100, 200, 300, and 400 ppb) in aqueous, which was compared to the same tetracycline concentrations prepared in aqueous synthesis suspension with >97% extraction, analyzed with LC-MS/MS.

Synthesis and Characterization of Lysine Modified Chitosan Magnetic Microspheres: A Novel Gene Delivery System

2007 ◽  
Author(s):  
Shuangyan Li ◽  
Lijuan Hao ◽  
Yong Yang ◽  
Lei Han ◽  
Peng Yao ◽  
...  
Keyword(s):  
Single Photon ◽  
Average Particle Size ◽  
Photon Emission ◽  
Magnetic Microspheres ◽  
Gene Carrier ◽  
Precipitation Method ◽  
Emission Computed Tomography ◽  
Average Particle ◽  
Modified Chitosan ◽  
New Formulation

This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer lysine modified chitosan (CS-lys) as gene carrier. Lysine modified chitosan was synthesized by performing carboxyl of lysine and amido of chitosan in the presence of 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride (EDC). The absolute chemistry of the Lysine modified chitosan obtained were characterized using IR and 1H NMR, respectively. The results indicated that many amines of chitosan were modificatied with lysine, and optimized the correlation conditions. The lysine modified chitosan magnetic microspheres were preprated by co-precipitation method. The characterizations of microspheres were measured by TEM, XPS, Dynamic Laser Light Scattering, Vibrating Samples Magnetometer, and X-ray diffraction. The resultes indicated the average particle size was about 100nm and have good superparamagnetic property. The cytotoxicity of CS-lys MNPs was investigated with U293 cells. The results showed that the CS-lys MNPs retained of low toxicity; additionally, combination of DNA and CS-lys MNPs was observed from agarose gel electrophoresis, suggesting that the CS-lys MNPs could be a novel magnetic targeting gene carrier. We also studied the ability of complexes of CS-lys MNPs and DNA crossing BBB in rats by single photon emission computed tomography (SPECT).

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