scholarly journals Statins Accelerated Arthritic Incidence: Ex vivo Antioxidant and Histopathological Analysis

2021 ◽  
Vol 23 (09) ◽  
pp. 902-910
Author(s):  
Litty Joseph ◽  
◽  
Jithin Mathew ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Ex Vivo ◽  
Supernatant Fraction ◽  
Tissue Homogenate ◽  
Histopathological Analysis ◽  
Activity Assay ◽  
Increased Risk ◽  
Statin Use

Statins are conventionally used medicaments for the treatment of hyperlipidemia and hypertension. Apart from the aforementioned pharmacological action, statin use was associated with increased risk for developing rheumatoid arthritis due to its immunomodulatory property. The present study focused to demonstrate whether statin use accelerated arthritic incidence. The Ex vivo antioxidant study was performed using Lipid peroxidation (LPO) and Superoxide dismutase (SOD) activity assay. Both the Antioxidant study was evaluated from tissue homogenates. Each homogenate was centrifuged, and the resulting supernatant fraction was used to determine the enzyme activity. The histopathological analysis of ankle joints was conducted by hematoxylin eosin staining technique. The result obtained from Lipid peroxidation study showed that both Pitavastatin and Lovastatin treated tissues exhibited significant increase (p<0.001) in enzyme activity due to the elevated level of malonaldehyde formation. The Superoxide dismutase activity assay of both test drugs on tissue homogenate was found that significant decrease (p<0.001) in SOD level contributed cartilage degradation due to the generation of free radicals. The histopathological analysis also revealed that scattered inflammatory cells destructs the normal articular structure in rat joint tissues showed the evidence of arthritic induction. The study confirmed that statins had a strong influence on arthritic onset by oxidative stress in patients with hyperlipidemia and hypertension.

scholarly journals Вплив хлориду кадмію на стан антиоксидантної системи у печінці щурів

2013 ◽  
pp. 102-103
Author(s):  
Б. В. Гутий
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride

Розкрито особливості антиоксидантної системиорганізму щурів за хронічного кадмієвого токсикозу.Встановлено, що хлорид кадмію у токсичній дозісприяє зниженню активності ферментної й нефер-ментної системи антиоксидантного захисту, на щовказує зниження ферментів глутатіонпероксидази,глутатіонредуктази, супероксиддисмутази, катала-зи та відновленого глутатіону у печінці щурів. Ре-зультати досліджень вказують на те, що хронічнийкадмієвий токсикоз призводить до посиленої акти-вації процесів ліпопероксидації. The features of the antioxidant system of rats with chronic cadmium toxicosiare disclosed. It wasresearched that cadmium chloride in toxic doses reduces enzyme activity of antioxidant system, asindicated by the decrease in enzyme glutathione peroxidase, hlutationreduktazy, superoxide dismutase,catalase and restored glutathione in the liver and blood of rats. The results indicate that chroniccadmium toxicosis leads to enhanced activation of lipid peroxidation.

scholarly journals Isolation and characterization of a rat cDNA clone encoding a secreted superoxide dismutase reveals the epididymis to be a major site of its expression

1993 ◽  
Vol 293 (1) ◽  
pp. 21-25 ◽  
Author(s):  
A C F Perry ◽  
R Jones ◽  
L Hall
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sequence Analysis ◽  
Cdna Clone ◽  
Northern Blot ◽  
Northern Blot Analysis ◽  
Major Site ◽  
Isolation And Characterization

Superoxide dismutase (SOD) plays a key role in combating loss of fertility of spermatozoa due to lipid peroxidation. Here we report the sequence of a cDNA encoding a secreted form of SOD isolated from a rat epididymal library. Northern-blot analysis indicates that the corresponding transcript is expressed principally in the cauda region of the epididymis, consistent with the high levels of SOD enzyme activity found in cauda-epididymidal plasma. Much lower levels of an identically sized transcript exist in all tissues examined, including placenta. PCR and subsequent sequence analysis of rat placental SOD strongly suggest that it is identical in sequence with epididymal SOD.

scholarly journals Порушення процесів вільнорадикального окиснення і антиоксидантного захисту в печінці при експериментальному алергічному альвеоліті та їх корекція тіотриазоліном

2015 ◽  
Author(s):  
М. А Пасічник
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Malonic Dialdehyde ◽  
Free Radical Oxidation ◽  
Antioxidant Protection ◽  
Allergic Alveolitis ◽  
Radical Oxidation ◽  
Peroxidase Oxidation ◽  
Experimental Allergic

ПОРУШЕННЯ ПРОЦЕСІВ ВІЛЬНОРАДИКАЛЬНОГО ОКИСНЕННЯ І АНТИОКСИДАНТНОГО ЗАХИСТУ В ПЕЧІНЦІ ПРИ ЕКСПЕРИМЕНТАЛЬНОМУ АЛЕРГІЧНОМУ АЛЬВЕОЛІТІ ТА ЇХ КОРЕКЦІЯ ТІОТРИАЗОЛІНОМ - У роботі показано, що експериментальний алергічний альвеоліт характеризується зростанням вмісту продуктів ліпопероксидаціі (дієнових кон’югатів і малонового діальдегіду) у всі періоди дослідження та активності ферментів супероксиддисмутази (СОД), каталази (КТ) і пероксидази (ПО) на 14 добу експерименту і суттєве зниження показників СОД, КТ і ПО на 34 і 44 доби цього модельного процесу захворювання та корегуючий вплив тіотриазоліну на дані показники.<br />НАРУШЕНИЕ ПРОЦЕССОВ СВОБОДНОРАДИКАЛЬНОГО ОКИСЛЕНИЯ И АНТИОКСИДАНТНОЙ ЗАЩИТЫ В ПЕЧЕНИ ПРИ ЭКСПЕРИМЕНТАЛЬНОМ АЛЛЕРГИЧЕСКОМ АЛЬВЕОЛИТЕ И КОРРЕКЦИЯ ИХТИОТРИАЗОЛИНОМ - В работе показано, что экспериментальный аллергический альвеолит характеризуется возростанием содержания продуктов липопероксидации (диеновых коньюгатов и малонового диальдегида) в печени во все периоды исследования и активности ферментов су- пероксиддисмутазы (СОД), каталазы (КТ) и пероксидазы (ПО), особенно на 14 сутки эксперимента и существенное снижение показателей СОД, КТ и ПО на 34 и 44 сутки этого модельного процесса заболевания и корригирующее влияние тио- триазолина на эти показатели.<br />DISTRUBANCES OF THE PROCESSES OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN LIVER AT EXPERIMENTAL ALLERGIC ALVEOLITIS AND THEIR CORRECTION WITH THIOTRIAZOLIN - The paper shows that experimental allergic alveolitis is characterized by increase of content of lipid peroxidation products (dienic conjugates and malonic dialdehyde) in all periods of study and enzyme activity of superoxide dismutase (SOD), catalase (CT) and peroxidase (PO) on the 14th day of the experiment and significant decrease of SOD, CT and PO on 34th and 44th days of this model process of disease and corrective influence of thiotriazolin on these indicators.<br />Ключові слова: пероксидне окиснення ліпідів, антиоксидантна система, тіотриазолін.<br />Ключевые слова: пероксидное окисление липидов, антиоксидантная система, тиотриазолин.<br />Key words: peroxidase oxidation of iipids, antioxidant system, thiotriazoiin.

scholarly journals Glycinebetaine mitigates drought stress-induced oxidative damage in pears

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0251389
Author(s):  
Tiequan Niu ◽  
Tianpeng Zhang ◽  
Yue Qiao ◽  
Pengfei Wen ◽  
Guangqian Zhai ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Drought Tolerance ◽  
Electrolyte Leakage ◽  
Antioxidant Enzyme Activity ◽  
Water Levels ◽  
Drought Treatment ◽  
Sod Activity ◽  
Treatment Groups

Glycinebetaine (GB) is an osmoprotectant found in plants under environmental stresses that incorporates drought and is associated with drought tolerance in several plants, such as the woody pear. However, how GB improves drought tolerance in pears remains unclear. In the current study, we explored the mechanism by which GB enhances drought tolerance of whole pear plants (Pyrus bretschneideri Redh. cv. Suli) supplied with exogenous GB. The results showed that on the sixth day after withholding water, levels of O2·−, H2O2, malonaldehyde (MDA) and electrolyte leakage in the leaves were substantially increased by 143%, 38%, 134% and 155%, respectively. Exogenous GB treatment was substantially reduced O2·−, H2O2, MDA and electrolyte leakage (38%, 24%, 38% and 36%, respectively) in drought-stressed leaves. Furthermore, exogenous GB induced considerably higher antioxidant enzyme activity in dry-stressed leaves than drought-stressed treatment alone on the sixth day after withholding water, such as superoxide dismutase (SOD) (201%) and peroxidase (POD) (127%). In addition, these GB-induced phenomena led to increased endogenous GB levels in the leaves of the GB 100 + drought and GB 500 + drought treatment groups by 30% and 78%, respectively, compared to drought treatment alone. The findings obtained were confirmed by the results of the disconnected leaf tests, in which GB contributed to a substantial increase in SOD activity and parallel dose- and time-based decreases in MDA levels. These results demonstrate that GB-conferred drought resistance in pears may be due in part to minimizing symptoms of oxidative harm incurred in response to drought by the activities of antioxidants and by reducing the build-up of ROS and lipid peroxidation.

The natural compound 7-epiclusianone inhibits superoxide dismutase activity inSchistosoma mansoni

2017 ◽  
Vol 92 (5) ◽  
pp. 535-543 ◽  
Author(s):  
M.S. Silva ◽  
A.P. Castro ◽  
A.T. de Castro ◽  
I.M.M. Souza ◽  
R.L. Martins-Souza ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Schistosoma Mansoni ◽  
Ex Vivo ◽  
Strong Interactions ◽  
Sod Activity ◽  
Docking Analysis ◽  
Novel Treatments ◽  
Polymerase Chain

AbstractSchistosomiasis – caused by trematodes from the genusSchistosoma– affects more than 200 million people worldwide. Growing resistance to therapy with praziquantel (PZQ) has encouraged the search for novel treatments against this neglected disease. The compound 7-epiclusianone (7-epi) – isolated from ‘bacupari’ (the fruit of theGracinia brasiliensistree) – has promising activity againstSchistosoma mansoni in vitro, damaging the parasite's tegument. However, the target and mechanism of action of 7-epi have not been identified. Here, we examined the possibility that 7-epi harms the tegument by inhibiting parasite superoxide dismutase (SOD), which protects the tegument from damage by reactive oxygen species produced by host immune cells. Molecular docking analysisin silicosuggested strong interactions between 7-epi andS. mansonicytosolic superoxide dismutase (SmCtSOD) at allosteric cavities.Schistosoma mansonicouples were cultivatedex vivowith 12.44–198.96 μm7-epi for 24 h, and then parasite extracts were tested for lipid peroxidation (as a surrogate for oxidative stress), and SOD activity and expression. Lipid peroxidation levels increased after incubation with concentrations ≥99.48 μm7-epi, and this compound reduced SOD activity at concentrations ≥24.87 μm. However, contact with 7-epi did not alter SOD expression, by quantitative real-time polymerase chain reaction (qRT-PCR). Our results show that the inhibition of SmCtSOD is partly responsible for the tegument detachment observed after incubation with 7-epi, but is not the only cause of the antiparasitic action of this compoundin vitro.

Sign in / Sign up

Export Citation Format

Share Document