scholarly journals Isolation and characterization of a rat cDNA clone encoding a secreted superoxide dismutase reveals the epididymis to be a major site of its expression

1993 ◽  
Vol 293 (1) ◽  
pp. 21-25 ◽  
Author(s):  
A C F Perry ◽  
R Jones ◽  
L Hall
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sequence Analysis ◽  
Cdna Clone ◽  
Northern Blot ◽  
Northern Blot Analysis ◽  
Major Site ◽  
Isolation And Characterization

Superoxide dismutase (SOD) plays a key role in combating loss of fertility of spermatozoa due to lipid peroxidation. Here we report the sequence of a cDNA encoding a secreted form of SOD isolated from a rat epididymal library. Northern-blot analysis indicates that the corresponding transcript is expressed principally in the cauda region of the epididymis, consistent with the high levels of SOD enzyme activity found in cauda-epididymidal plasma. Much lower levels of an identically sized transcript exist in all tissues examined, including placenta. PCR and subsequent sequence analysis of rat placental SOD strongly suggest that it is identical in sequence with epididymal SOD.

Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase

1993 ◽  
Vol 23 (3) ◽  
pp. 543-551 ◽  
Author(s):  
Cathal Wilson ◽  
Norbert Eller ◽  
Anton Gartner ◽  
Oscar Vicente ◽  
Erwin Heberle-Bors
Keyword(s):  
Map Kinase ◽  
Cdna Clone ◽  
Isolation And Characterization

Isolation and characterization of a cDNA clone for a novel serine-rich neutrophil protein

1990 ◽  
Vol 170 (2) ◽  
pp. 915-922 ◽  
Author(s):  
P. Bellavite ◽  
F. Bazzoni ◽  
M.A. Cassatella ◽  
K.J. Hunter ◽  
J.V. Bannister
Keyword(s):  
Cdna Clone ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of bleomycin-resistant clones of CHO cells

1979 ◽  
Vol 34 (3) ◽  
pp. 269-279 ◽  
Author(s):  
Sylvia Brabbs ◽  
J. R. Warr
Keyword(s):  
Enzyme Activity ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Tween 80 ◽  
The Other ◽  
Resistant Clone ◽  
Ovary Cells ◽  
Isolation And Characterization ◽  
Significant Difference

SUMMARYFive clones of Chinese hamster ovary cells with increased resistance to bleomycin have been isolated following ethylmethanesulphonate mutagenesis. Resistance was stable in three of the clones, but unstable in the other two. One of the stably resistant clones was cross resistant to unrelated drugs, and in contrast to the parental cells, its response to bleomycin was potentiated by tween 80. These two observations suggested a membrane alteration in the resistant clone. There was no significant difference in bleomycin-inactivating enzyme activity between the parental and resistant clones.

scholarly journals Molecular Characterization of Two Lactate Dehydrogenase Genes with a Novel Structural Organization on the Genome of Lactobacillus sp. Strain MONT4

2004 ◽  
Vol 70 (10) ◽  
pp. 6290-6295 ◽  
Author(s):  
Jennifer Weekes ◽  
Gülhan Ü. Yüksel
Keyword(s):  
Escherichia Coli ◽  
Lactate Dehydrogenase ◽  
Sequence Analysis ◽  
Molecular Characterization ◽  
Northern Blot ◽  
Structural Organization ◽  
Genomic Organization ◽  
The Individual

ABSTRACT Two lactate dehydrogenase (ldh) genes from Lactobacillus sp. strain MONT4 were cloned by complementation in Escherichia coli DC1368 (ldh pfl) and were sequenced. The sequence analysis revealed a novel genomic organization of the ldh genes. Subcloning of the individual ldh genes and their Northern blot analyses indicated that the genes are monocistronic.

scholarly journals Вплив хлориду кадмію на стан антиоксидантної системи у печінці щурів

2013 ◽  
pp. 102-103
Author(s):  
Б. В. Гутий
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride

Розкрито особливості антиоксидантної системиорганізму щурів за хронічного кадмієвого токсикозу.Встановлено, що хлорид кадмію у токсичній дозісприяє зниженню активності ферментної й нефер-ментної системи антиоксидантного захисту, на щовказує зниження ферментів глутатіонпероксидази,глутатіонредуктази, супероксиддисмутази, катала-зи та відновленого глутатіону у печінці щурів. Ре-зультати досліджень вказують на те, що хронічнийкадмієвий токсикоз призводить до посиленої акти-вації процесів ліпопероксидації. The features of the antioxidant system of rats with chronic cadmium toxicosiare disclosed. It wasresearched that cadmium chloride in toxic doses reduces enzyme activity of antioxidant system, asindicated by the decrease in enzyme glutathione peroxidase, hlutationreduktazy, superoxide dismutase,catalase and restored glutathione in the liver and blood of rats. The results indicate that chroniccadmium toxicosis leads to enhanced activation of lipid peroxidation.

Isolation and characterization of a soybean cDNA clone encoding the plastid form of aspartate aminotransferase

1993 ◽  
Vol 21 (6) ◽  
pp. 993-1009 ◽  
Author(s):  
Gregory J. Wadsworth ◽  
Simos M. Marmaras ◽  
Benjamin F. Matthews
Keyword(s):  
Cdna Clone ◽  
Aspartate Aminotransferase ◽  
Isolation And Characterization

Isolation and Characterization of Hirudin Isoinhibitors and Sequence Analysis of Hirudin PA

1986 ◽  
Vol 367 (2) ◽  
pp. 803-812 ◽  
Author(s):  
Johannes DODT ◽  
Werner MACHLEIDT ◽  
Ursula SEEMÜLLER ◽  
Reinhard MASCHLER ◽  
Hans FRITZ
Keyword(s):  
Sequence Analysis ◽  
Isolation And Characterization
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