scholarly journals Laboratory Practices for Purification of Polysaccharide

2021 ◽  
Vol 23 (10) ◽  
pp. 174-178
Author(s):  
Ramu Govindan ◽  
Keyword(s):  
Hydrochloric Acid ◽  
Trichloroacetic Acid

The aim of the present work is to purify the mucilage polysaccharide extracted from linseed by deproteinization using Trichloroacetic acid (TCA) and Hydochloric acid (Hcl). 10% w/v TCA deproteinized more than 80% when the pH of the polysaccharide solution was 3. TCA can be considered as an better deproteinizing agent when compared to hydrochloric acid as evidenced by the highest deproteinization efficiency (88.57%)

An alternative method for assaying cerebrospinal fluid protein in the presence of methotrexate.

1988 ◽  
Vol 34 (10) ◽  
pp. 2091-2092 ◽  
Author(s):  
L M Kasper ◽  
W R Moorehead ◽  
T O Oei ◽  
M Markanich
Keyword(s):  
Cerebrospinal Fluid ◽  
Hydrochloric Acid ◽  
Trichloroacetic Acid ◽  
Dilute Hydrochloric Acid ◽  
Constant Amount ◽  
Manual Method ◽  
Turbidimetric Method ◽  
Methotrexate Concentration ◽  
Cerebrospinal Fluid Protein ◽  
Positive Interference

Abstract Therapeutic concentrations of methotrexate can cause significant positive interference in cerebrospinal fluid (CSF) protein values when assayed in the Du Pont aca. Conversely, our modified turbidimetric method, in which trichloroacetic acid (TCA) plus a sample blank containing dilute hydrochloric acid is used in place of TCA, exhibits little or no interference from methotrexate. This was verified by assaying solutions that contained a constant amount of protein (approximately 430 mg/L) and various amounts of methotrexate (0.0-2.3 x 10(-4) mol/L) by both the Du Pont aca and the manual turbidimetric method. As expected, the aca results showed increasing protein values with increasing methotrexate, whereas the manual method gave results approximating the expected protein value irrespective of the methotrexate concentration.

The Estimation of Lithium in Serum

1971 ◽  
Vol 118 (543) ◽  
pp. 225-226 ◽  
Author(s):  
H. I. Coombs
Keyword(s):  
Hydrochloric Acid ◽  
Trichloroacetic Acid ◽  
Hospital Laboratory ◽  
Flame Photometer ◽  
Subsequent Heating

Serum lithium estimation has recently assumed great importance in psychiatry. It can be successfully undertaken in any ordinary hospital laboratory equipped with a simple flame photometer such as the EEL (Evans Electroselenium Limited). It has been found that de-proteinization of serum is very satisfactorily achieved by addition of hydrochloric acid and subsequent heating. This avoids the retention of lithium in the precipitate which is associated with the use of trichloroacetic acid and of ethanol. The method is simple for the technician and very reliable and accurate.

Extraction and Deproteinization of Mannan Oligosaccharides

2010 ◽  
Vol 65 (5-6) ◽  
pp. 387-390 ◽  
Author(s):  
Gang Huang ◽  
Qin Yang ◽  
Zhong B. Wang
Keyword(s):  
Cell Wall ◽  
Hydrochloric Acid ◽  
Yeast Cell ◽  
Trichloroacetic Acid ◽  
The Other ◽  
Yeast Cell Wall ◽  
Mannan Oligosaccharide ◽  
Acid Method ◽  
Mannan Oligosaccharides

Extraction of mannan oligosaccharides from the yeast cell wall and methods of deproteinization were studied. We extracted crude mannan oligosaccharides by the dilute alkali- Sevage method. The percentages of deproteinization and mannan oligosaccharide loss were compared as indexes using the Sevage method, trichloroacetic acid method, and hydrochloric acid method. The results showed that the hydrochloric acid method exhibited the highest percentage of deproteinization, but only a little higher percentage of mannan oligosaccharide loss than the other two methods.

scholarly journals Automated Trichloroacetic Acid Precipitation Method for Urine Total Protein

1987 ◽  
Vol 24 (2) ◽  
pp. 140-144 ◽  
Author(s):  
C K Cheung ◽  
Y T Mak ◽  
R Swaminathan
Keyword(s):  
Hydrochloric Acid ◽  
Total Protein ◽  
Trichloroacetic Acid ◽  
Acid Precipitation ◽  
Precipitation Method ◽  
Protein Determination ◽  
Standard Curve ◽  
Analytical Recovery ◽  
Assay Precision ◽  
Method R

Urine total protein determination by a trichloroacetic acid (TCA) precipitation method was automated on a Cobas Bio centrifugal analyser. The assay measures the turbidity at 420 nm when a 50 μL sample is mixed with 150 μL of 30 g/L TCA at 25°C. Samples up to 5 g/L can be measured by this method and interference due to pigments and turbidity can be minimised by running a blank with 1.25% hydrochloric acid (HCl) instead of TCA. The standard curve is stable and can be stored in the microcomputer and used again. Within-assay precision (CV) varied from 2 to 4.6% and between-assay precision varied from 1.8 to 5.4%. Analytical recovery ranged from 95 to 106.5% and the results correlated well with those obtained by a manual TCA method ( r=0.98). The method is easy to perform and is considerably faster than the manual procedure, thus saving time and providing a faster turnround time.

scholarly journals MAYER'S TANNIC ACID-FERRIC CHLORIDE STAIN FOR MUCINS

1973 ◽  
Vol 21 (1) ◽  
pp. 56-64 ◽  
Author(s):  
PHILIP PIZZOLATO ◽  
R. D. LILLIE
Keyword(s):  
Aqueous Solution ◽  
Hydrochloric Acid ◽  
Tannic Acid ◽  
Ferric Chloride ◽  
Trichloroacetic Acid ◽  
Acetyl Chloride ◽  
Colored Compound ◽  
Formalin Fixed

Tannic acid in aqueous solution is bound to mucins in formalin-fixed and formalin-free fixed tissues and its presence can be detected with ferric chloride as a dark gray, blue-black to black complex. This colored compound is readily extracted by acids and some chelating and bleaching agents and is changed to a reddish brown by alkalis. Hydrolysis in 1.2 N hydrochloric acid at 60°C for 4 hr or hot trichloroacetic acid prevents the tannin-iron reaction. Acetyl chloride or bromide is able to inhibit the binding of tannic acid to the mucosubstances and saponification restores the characteristic reaction. Several mechanisms for the attachment of tannic acid to the mucins appear possible.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

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