scholarly journals MAYER'S TANNIC ACID-FERRIC CHLORIDE STAIN FOR MUCINS

1973 ◽  
Vol 21 (1) ◽  
pp. 56-64 ◽  
Author(s):  
PHILIP PIZZOLATO ◽  
R. D. LILLIE
Keyword(s):  
Aqueous Solution ◽  
Hydrochloric Acid ◽  
Tannic Acid ◽  
Ferric Chloride ◽  
Trichloroacetic Acid ◽  
Acetyl Chloride ◽  
Colored Compound ◽  
Formalin Fixed

Tannic acid in aqueous solution is bound to mucins in formalin-fixed and formalin-free fixed tissues and its presence can be detected with ferric chloride as a dark gray, blue-black to black complex. This colored compound is readily extracted by acids and some chelating and bleaching agents and is changed to a reddish brown by alkalis. Hydrolysis in 1.2 N hydrochloric acid at 60°C for 4 hr or hot trichloroacetic acid prevents the tannin-iron reaction. Acetyl chloride or bromide is able to inhibit the binding of tannic acid to the mucosubstances and saponification restores the characteristic reaction. Several mechanisms for the attachment of tannic acid to the mucins appear possible.

Studying dispersed and rheological properties of hydrocarbon-containing acid emulsions and their efficiency in removing asphalt-resin-paraffin deposits

2020 ◽  
pp. 128-139
Author(s):  
M. Yu. Shumakher ◽  
V. V. Konovalov ◽  
A. P. Melnikov
Keyword(s):  
Aqueous Solution ◽  
Hydrochloric Acid ◽  
Rheological Properties ◽  
Reaction Rate ◽  
Experimental Studies ◽  
Efficient Removal ◽  
Reservoir Properties ◽  
Hydrocarbon Solvents ◽  
Formation Zone ◽  
Effect Of The Composition

Currently, the treatment of the bottomhole formation zone with acidic compositions is one of the most common methods to intensify the oil inflow. The use of various modified acid compositions increases the efficiency of acid treatments on the bottomhole formation zone. Acid compositions, including those containing hydrocarbon solvents, which contribute to more efficient removal of organic colmatants, affect the reaction rate of the reagent with the rock and processing equipment, change the reservoir properties, etc.The article presents the results of experimental studies, which are aimed at establishing the effect of the composition of hydrocarbon-containing acidic emulsions consisting of an aqueous solution of hydrochloric acid, toluene and Neonol AF 9-10 on their dispersed and rheological properties, as well as their efficiency in removing paraffin deposits.

An alternative method for assaying cerebrospinal fluid protein in the presence of methotrexate.

1988 ◽  
Vol 34 (10) ◽  
pp. 2091-2092 ◽  
Author(s):  
L M Kasper ◽  
W R Moorehead ◽  
T O Oei ◽  
M Markanich
Keyword(s):  
Cerebrospinal Fluid ◽  
Hydrochloric Acid ◽  
Trichloroacetic Acid ◽  
Dilute Hydrochloric Acid ◽  
Constant Amount ◽  
Manual Method ◽  
Turbidimetric Method ◽  
Methotrexate Concentration ◽  
Cerebrospinal Fluid Protein ◽  
Positive Interference

Abstract Therapeutic concentrations of methotrexate can cause significant positive interference in cerebrospinal fluid (CSF) protein values when assayed in the Du Pont aca. Conversely, our modified turbidimetric method, in which trichloroacetic acid (TCA) plus a sample blank containing dilute hydrochloric acid is used in place of TCA, exhibits little or no interference from methotrexate. This was verified by assaying solutions that contained a constant amount of protein (approximately 430 mg/L) and various amounts of methotrexate (0.0-2.3 x 10(-4) mol/L) by both the Du Pont aca and the manual turbidimetric method. As expected, the aca results showed increasing protein values with increasing methotrexate, whereas the manual method gave results approximating the expected protein value irrespective of the methotrexate concentration.

DETERMINATION OF TRACE AMOUNTS OF SILVER IN GALENA ORES

1960 ◽  
Vol 38 (9) ◽  
pp. 1488-1494 ◽  
Author(s):  
E. J. Bounsall ◽  
W. A. E. McBryde
Keyword(s):  
Aqueous Solution ◽  
Nitric Acid ◽  
Sodium Chloride ◽  
Hydrochloric Acid ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Silver Content ◽  
Aqueous Sodium Chloride Solution ◽  
Aqueous Sodium

An analytical method is described for the determination of microgram amounts of silver in galena ores, based on the "reversion" of silver dithizonate. Silver is separated from relatively large amounts of lead by extraction as dithizonate into chloroform from an aqueous 1:99 nitric acid solution. Separation from mercury, which is also extracted under these conditions and would, if present, interfere in the analysis, is achieved by reverting the dithizonate solution with a 5% aqueous sodium chloride solution which is also 0.015 molar in hydrochloric acid. Following dilution of this aqueous solution and adjustment of pH, silver is again extracted into chloroform as the dithizonate, and determined absorptiometrically. Analyses of a number of galena ore samples showed a precision of within 3% for a silver content ranging from 0.03 to 0.4%.Some other methods for isolating silver from these samples, which were tried but found unsatisfactory, are discussed.

A Modification of Mayer's Tannic Acid-Ferric Chloride Staining Method for Demonstrating Cellular Membranous Systems for Light Microscopy

2000 ◽  
Vol 75 (1) ◽  
pp. 33-40
Author(s):  
Shinji Matsui ◽  
Karou Hatanaka ◽  
Hiroshi Watanabe ◽  
Yumi Asano ◽  
Hidehiro Yamagata ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Tannic Acid ◽  
Ferric Chloride ◽  
Staining Method
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