scholarly journals OCORRÊNCIA DE OTITE EXTERNA EM CÃES E GATOS ATENDIDOS EM UMA CLÍNICA ESCOLA DE MEDICINA VETERINÁRIA

2021 ◽  
Author(s):  
Alana Maria Tavares Barros ◽  
Nilo Ricardo Vasconcelos Torquarto ◽  
Millena Marinho Santos ◽  
Ana Beatriz Pires Curvelo Martins Tenório Carmo ◽  
Roberto Rômulo Ferreira Da Silva ◽  
...  
Keyword(s):  
Labrador Retriever ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Malassezia Pachydermatis ◽  
De Medicina

Introdução: A otite externa caracteriza-se como uma inflamação aguda ou crônica do epitélio do meato auditivo, sendo uma das afecções mais comuns na rotina da clínica médica dos animais. Algum dos fatores que influenciam no aparecimento das otites são: morfologia das orelhas, limpeza excessiva do conduto auditivo, doenças sistêmicas e alterações climáticas. Objetivos: Objetivou-se com este trabalho, realizar um estudo retrospectivo da ocorrência de otite em cães e gatos atendidos na clínica escola de Medicina Veterinária. Material e métodos: No período de 2019 a 2021, foi realizado a partir de análises das fichas clínicas um levantamento dos casos diagnosticados de otite de cães e gatos atendidos na clínica médica de pequenos animais do Centro Universitário Cesmac, Marechal Deodoro, Alagoas. Foram analisados a espécie, sexo e raça dos animais acometidos, realizando a identificação dos microrganismos em ambos ouvidos, feita por exame microbiológico, com auxílio de swab estéril e meio de transporte Stuart. Resultados: Foram colhidas 26 amostras de secreção de conduto auditivo esquerdo e direito de 15 animais (13 cães e 2 gatos). Destes 13 cães, 53,84% (7/13) eram fêmeas; 46,16% (6/13) eram machos, dos 2 gatos, 100% (2/2) eram machos. Dos 13 cães, 46,15% (6/13) eram SRD; 23,07% (3/13) eram Dachshund; 15,38% (2/13) eram Pastor-Alemão; 07,69% (1/13) eram Pug e 07,69% (1/13) eram Labrador Retriever; dos 2 gatos, 100% (2/2) eram SRD. Do total de amostras avaliadas, foram observados os microrganismos Streptococcus sp. 15,38% (4/26); 11,53% (3/26) Staphylococcus sp + Klebisiella sp; 11,53% (3/26) Proteus sp + Staphylococcus sp; 11,53% (3/26) Bacillus sp; 07,69% (2/26) Pseudomonas sp; 07,69% (2/26) Staphylococcus sp + Streptococcus sp; 07,69% (2/26) Proteus sp; 07,69% (2/26) Bacillus sp + Staphylococcus sp; 07,69% (2/26) Malassezia pachydermatis; 07,69% (2/26) Pseudomonas sp. + Proteus sp + Staphylococcus sp; 03,84% (1/26) Proteus sp + Pseudomonas sp. Conclusão: Diante dos resultados, foi possível observar que o agente mais prevalente foi Streptococcus sp. Houve uma maior ocorrência de infecções em cães quando comparado com os gatos, não sendo observada predileção sexual. Ressalta-se a importância da realização da cultura e antibiograma para que seja feito o tratamento mais adequado para combater a infecção.

scholarly journals LEVANTAMENTO DE AGENTES BACTERIANOS ISOLADOS DE AMOSTRAS DE CÃES E GATOS COM AFECÇÕES CLÍNICAS

2021 ◽  
Author(s):  
Ana Beatriz Pires Curvelo Martins Tenório Carmo ◽  
Nilo Ricardo Vasconcelos Torquato ◽  
Millena Marinho Santos ◽  
Alana Maria Tavares Barros ◽  
Roberto Rômulo Ferreira Da Silva ◽  
...  
Keyword(s):  
Labrador Retriever ◽  
Bacillus Sp ◽  
De Medicina

Introdução: Infecções bacterianas são de grande importância na rotina clínica de pequenos animais, sendo as mais comuns, as infecções de conduto auditivo e infecções urinárias. Objetivo: Objetivou-se com este trabalho verificar os agentes das afecções clínicas em cães e gatos atendidos na Clínica Escola de Medicina Veterinária. Material e métodos: No período de fevereiro de 2020 a junho de 2021, realizou-se um levantamento das fichas dos casos de afecções clínicas em cães e gatos atendidos na Clínica Escola do Centro Universitário Cesmac, Marechal Deodoro, Alagoas. A partir das análises das fichas, obteve-se dados como raça, sexo, idade, além disso foi possível identificar os principais microrganismos isolados por meio de exame microbiológico das amostras colhidas. Resultados: Neste período foram atendidos 11 animais, sendo seis cães (54,54%) e cinco felinos (45,46%). Os cães possuíam idades entre um mês a 10 anos, e gatos predominantemente jovens de quatro meses a três anos. No agrupamento das raças, os cães tiveram predominância de animais SRD (33,33%) seguindo das raças Pug (16,66%), Labrador Retriever (16,66%), Dachshund (16,66%) e Pastor-alemão (16,66%). Já os felinos SRD. tiveram predominância de 100%. Destes 11 animais, foram colhidas 17 amostras (11 amostras de secreção otológica, duas amostras de trato urinário, uma de liquido intracraniano, uma de secreção nasal, uma de aspirado de lesão e uma de fragmento de pele). As infecções de conduto auditivo e trato urinário foram as mais observadas na rotina da clínica médica. Houve maior ocorrência de Staphylococcus sp. 23,52% (4/17) e Streptococcus sp. 23,52% (4/17) nas amostras clínicas, seguido de Staphylococcus + Proteus sp. + Pseudomonas 11,76% (2/17); Bacillus sp. 11,76% (2/17); Staphylococcus sp. + Streptococcus sp. 5,88% (1/17) ; Streptococcus sp. + Proteus sp. 5,88% (1/17); e bactéria Gram Negativa 5,88% (1/17). Em apenas duas amostras não houve o crescimento bacteriano, sendo observada a presença do fungo Malassezia sp (11,76%). Conclusão: Observou-se que as bactérias Staphylococcus sp. e Streptococcus sp. foram as mais prevalentes nas afecções clínicas. Devido ao aumento das infeções bacterianas, ressalta-se a importância de realizar a cultura bacteriana e o antibiograma, com intuito de instituir o tratamento mais eficaz para cada tipo de afecção.

Enhanced Toxicity of Bacillus thuringiensis Subspecies kurstaki and aizawai to Black Cutworm Larvae (Lepidoptera: Noctuidae) With Bacillus sp. NFD2 and Pseudomonas sp. FNFD1

2011 ◽  
Vol 104 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Tamer A. Mashtoly ◽  
Assem Abolmaaty ◽  
Mohamed El-Said El-Zemaity ◽  
Mohamed I. Hussien ◽  
Steven R. Alm
Keyword(s):  
Bacillus Thuringiensis ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Black Cutworm ◽  
Lepidoptera Noctuidae

scholarly journals Sorção de cobre e manganês por bactérias rizosféricas do trigo

2001 ◽  
Vol 31 (6) ◽  
pp. 947-951 ◽  
Author(s):  
Marcio Voss ◽  
Robert Wayne Stefen Phillip Thomas
Keyword(s):  
Pseudomonas Sp ◽  
Bacillus Sp

A bioacumulação de metais por microrganismos se deve principalmente a fenômenos de superfície, ocorrendo adsorção, de forma estequiométrica, com os radicais aniônicos dos envoltórios celulares, seguido ou não de precipitação dos metais. Para estudar condicionantes da sorção de metais por bactérias vivas, quantificou-se o Cu2+ e Mn2+ retirados por um Bacillus sp. e uma Pseudomonas sp., isolados da rizosfera de trigo, de uma solução de cloreto dos metais, determinando-se a quantidade de metal restante no sobrenadante, após centrifugação. Usou-se delineamento experimental inteiramente casualizado, com três repetições. Ensaiaram-se efeito dos teores de Cu2+ e Mn2+, do pH e do tempo de crescimento bacteriano. O Bacillus sorveu mais Cu2+ e Mn2+ do que Pseudomonas. em todas as concentrações desses metais. A sorção de Cu2+ por ambas as bactérias apresentou maiores incrementos do que Mn2+ com aumento dos teores desses metais na solução. A alteração do pH 5,0 para 3,0 diminuiu a sorção dos dois metais. Com o tempo de cultivo de 90 horas a Pseudomonas apresentou maior sorção de cobre e de manganês do que no tempo de 16 horas. Os resultados obtidos assemelham-se aos fenômenos de troca de cátions em colóides.

scholarly journals Characterization and Lipolytic Activity of Bacteria Isolates from Freshwater Clam (Mercenaria Mercenaria) in Bayelsa State, Nigeria

2020 ◽  
pp. 1-4
Author(s):  
Opara C N ◽  
◽  
Anumudu C K ◽  
Keyword(s):  
Protein Content ◽  
Culture Media ◽  
Lipolytic Activity ◽  
Cell Mass ◽  
Mercenaria Mercenaria ◽  
Total Protein Content ◽  
Growth Media ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Freshwater Clam

Lipases form an important group of relevant enzymes which have applications in various fields including; food, pharmaceutical, detergent, textile and cosmetic industries. Lipases can be produced from diverse sources including microorganisms. This study evaluated the potential of bacteria isolates from fresh-water clam Mercenaria Mercenaria to produce lipolytic enzymes. Ten samples of Clam (Mercenaria Mercenaria) were screened for the presence of lipase producing bacteria using classical culture methods. Eleven bacteria species were obtained, of which six (Actinomyces sp., E. coli, Bacillus sp., Pseudomonas sp., Clostridium sp. and Klebsiella sp.) produced lipases that had lipolytic activity in breaking down olive oil used in media supplementation. The best culture media and conditions for optimal production of lipases was studied and it was shown that supplementation of growth media with 2% dextrose at neutral pH gave the greatest yield of lipases when lipase producing isolates were grown in shake flasks. Measurement of biomass by culture and turbidimetric methods indicates that the highest cell mass was recorded by Pseudomonas sp at 7.8 x 105 CFU/ml, closely followed by Actinomyces sp. and Bacillus sp., at 6.2 x 105 CFU/ml and 5.3 x 105 respectively. The produced lipases were partially purified by precipitating with ammonium sulphate followed by dialysis. The total protein content of produced lipases was evaluated by the Lowry’s method, showing that estimated protein content followed the same trend as cell biomass with the highest recorded by Pseudomonas sp. at 1.53mg/ml, followed by Actinomyces sp. and Bacillus sp. at 1.47mg/ml and 1.32mg/ml respectively. The results obtained in this study shows that isolates obtained from freshwater clam can produce potent lipases which can be employed for industrial, food and other diverse uses

scholarly journals Skrining Dan Seleksi Bakteri Simbion Spons Penghasil Enzim Ekstraseluler Sebagai Agen Bioremediasi Bahan Organik Dan Biokontrol Vibriosis Pada Budidaya Udang

2016 ◽  
Vol 19 (1) ◽  
pp. 11 ◽  
Author(s):  
Wilis Arii Setyati ◽  
Ahmad Saddam Habibi ◽  
Subagiyo Subagiyo ◽  
Ali Ridlo ◽  
Nirwani S ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Vibrio Alginolyticus ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Acinetobacter Sp

Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp. Kata kunci:  Bakteri simbion spons, enzim ekstraseluler, bioremediasi, vibriosis

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

Occurrence of antibiotic resistant bacteria in some shrimp farms of Bangladesh

2013 ◽  
Vol 41 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Farzana Ashrafi Neela ◽  
Md Atiquer Rahman ◽  
MST Nasrin Akhter Banu ◽  
M Habibur Rahman ◽  
Hiroyuki Ohta ◽  
...  
Keyword(s):  
Brackish Water ◽  
Water Samples ◽  
Farming System ◽  
Shrimp Farming ◽  
Resistant Bacteria ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Antibiotic Resistant ◽  
Acinetobacter Sp ◽  
Shrimp Farms

Tetracycline (TC) and ampicillin (AMP) resistant bacteria were identified from both fresh and brackish water shrimp farming system, in Bangladesh. Among 78 isolates from freshwater samples, 14.10, 17.95 and 23.8% were found resistant to TC, AMP and TC plus AMP, respectively. On the other hand, isolates from the brackish water samples, the percentages of TC, AMP and TC plus AMP resistant isolates were 10.25, 12.82 and 15.38, respectively. In freshwater samples, the highest percentage of resistant bacteria was Bacillus sp. (38.9), Pseudomonas sp. (22.2), Staphylococcus sp. (16.7), Acinetobacter sp. (11.1), Brevibacillus sp. (5.5) and Enterobacter sp. (5.5). While in brackish water samples dominant resistant bacteria was Bacillus sp. (50) followed by Pseudomonas sp. (16.7), Acinetobacter sp. (16.7), Enterobacter sp. (8.3) and Microvirgula sp. (8.3). DOI: http://dx.doi.org/10.3329/bjb.v41i2.13449 Bangladesh J. Bot. 41(2): 197-200, 2012 (December)

scholarly journals Pola Kuman Penyebab Ventilator Associated Pneumonia(VAP) dan Sensitivitas Terhadap Antibiotik di RSAB Harapan Kita

Sari Pediatri ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 384
Author(s):  
Retno Widyaningsih ◽  
Latre Buntaran
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Clinical Laboratory ◽  
Enterobacter Aerogenes ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Ventilator Associated Pneumonia ◽  
Pseudomonas Sp ◽  
Bacillus Sp

Latar belakang. Peningkatan resistensi antibiotik di antara bakteri penyebab pneumonia nosokomial yang didapat di rumah sakit telah banyak dilaporkan. Jika kita tidak mengenal pola kepekaan kuman di suatu rumah sakit akan menyulitkan pemberian terapi empiris awal. Tujuan. Mengetahui profil kuman penyebab pneumonia yang didapat di rumah sakit pada anak serta uji sensitivitas terhadap beberapa antibiotik.Metode. Studi deskriptif retrospektif dengan sumber data yang berasal dari rekam medis Laboratorium Mikrobiologi RSAB Harapan Kita periode Januari hingga Juni 2010. Spesimen adalah semua spesimen saluran respiratorik dari pasien dengan diagnosis pneumonia yang dirawat. Biakan dan uji resistensi dilakukan menurut standar National Committee for Clinical Laboratory Standards(NCCLS).Hasil. Didapatkan 116 spesimen biakan dan di antaranya 4 (3,4%) steril. Dari 112 biakan positif, 79.5% di antaranya adalah bakteri gram negatif berturut-turut dari yang paling dominan adalah Pseudomonas sp.(22,4%), Pseudomonas aeruginosa(18,1%), Stenotrophomonas maltophilia(9.5%), Serratia marcescens(8,6%),Enterobacter aerogenes(7,8%), Klebsiella pneumonia, Bacillus sp., dan Escherichia coli(masing-masing 5,2%). Golongan Pseudomonasmemiliki sensitivitas terhadap ceftazidime, amikacin serta netilmicin.Kesimpulan. Basil gram negatif aerob (79,5%) merupakan mikroorganisme penyebab yang paling dominan. Ceftazidime, diikuti terhadap amikacin serta netilmicin masih mempunyai sensitivitas yang tinggi sehingga dapat dipakai sebagai terapi awal VAP.

scholarly journals Isolasi dan Identifikasi Bakteri Kitinolitik yang terdapat pada Cangkang Lobster Air Tawar (Cherax Quadricarinatus) [Isolation and Identification of Chytinolitic Bacteria from The Crayfish (Cherax Quadricarinatus) Shell]

2019 ◽  
Vol 8 (1) ◽  
pp. 16
Author(s):  
Hari Suprapto ◽  
Sudarno Sudarno ◽  
Istikhara Mentari Tito
Keyword(s):  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Test Medium ◽  
Clear Zone ◽  
Chitinolytic Activity ◽  
Isolation And Identification ◽  
Cherax Quadricarinatus ◽  
Chitinolytic Bacteria ◽  
Bacterium Species ◽  
Observation Method

AbstrakIndonesia memiliki potensi perikanan yang sangat tinggi, salah satunya adalah lobster. Ekspor lobster air tawar cenderung meningkat tiap tahun. Total ekspor hasil lobster budidaya mencapai 94.511 ton/tahun. Pangsa pasar lobster air tawar tidak hanya terbatas di dalam negeri saja tetapi juga ke luar negeri. Penelitian ini bertujuan untuk mengetahui adanya bakteri kitinolitik dan juga jenis-jenis bakteri kitinolitik yang terdapat pada cangkang lobster air tawar. Penelitian ini menggunakan Penelitian ini menggunakan metode observasi untuk mengetahui adanya bakteri kitinolitik yang ada pada cangkang lobster air tawar. Data yang digunakan dalam penelitian ini adalah secara deskriptif. Data yang diperoleh dianalisis secara deskriptif yaitu penyajian data tentang morfologi dan karakteristik dari bakteri kitinolitik yang diisolasi dari cangkang lobser air tawar (Cherax quadricarinatus) dan dibandingkan dengan morfologi dan karakteristik bakteri kitinolitik dengan literatur yang sesuai. Hasil penelitian ini adalah diperoleh bahwa bakteri yang diisolasi dari cangkang lobster air tawar (Cherax quadricarinatus) dapat tumbuh dan berkembang pada media uji. Kemudian hasil uji hidrolisis bakteri kitinolitik ditandai dengan adanya zona bening yang dihasilkan dari bakteri tersebut. Dari hasil pengujian tersebut didapatkan jenis – jenis bakteri yaitu Aeromonas sp., Bacillus sp. dan Pseudomonas sp. AbstractIndonesia have highest potential of fishery. One of them is Lobster. It has increasing slightly, approximately 94.511 ton/years. The aims this research to determine the presence of chitinolytic bacteria and identify its species in crayfish shells. This research was performed via observation method to determine the presence of chitinolytic bacteria, which are exist in crayfish (cherax quadricarinatus) shell. The data used in this research is descriptive. The data descriptively analyzed represented the morphology and characteristics of chitinolytic bacteria isolated from crayfish (Cherax quadricarinatus) and comparison the morphology and characteristics of chitinolytic bacteria with appropriate literature. These results obtained that the bacteria isolated from crayfish (Cherax quadricarinatus) shell can grow and develop in the test medium. Then, chitinolytic activity was signed by the formation of clear zone on the test medium. The results obtained several bacterium species including Aeromonas sp., Bacillus sp. and Pseudomonas sp. 

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