scholarly journals Clinical and anatomical approach using Sihler's staining technique (whole mount nerve stain)

2011 ◽  
Vol 44 (1) ◽  
pp. 1 ◽  
Author(s):  
Sung-Yoon Won ◽  
Da-Hye Kim ◽  
Hun-Mu Yang ◽  
Jong-Tae Park ◽  
Hyun-Ho Kwak ◽  
...  
Keyword(s):  
Staining Technique ◽  
Whole Mount ◽  
Sihler’S Staining

scholarly journals A Review of Intramucosal and Intramuscular Whole Mount Nerve Staining Technique (Sihler's staining)

2010 ◽  
Vol 32 (1) ◽  
pp. 37-46
Author(s):  
원성윤 ◽  
Mi-Sun Hur ◽  
김희진 ◽  
Jongtae Park ◽  
곽현호 ◽  
...  
Keyword(s):  
Staining Technique ◽  
Whole Mount ◽  
Sihler’S Staining ◽  
Nerve Staining

Three-dimensional observation of the mandible and accessory mental nerve using Sihler's staining technique

2014 ◽  
Vol 72 (9) ◽  
pp. e67-e68
Author(s):  
J. Iwanaga ◽  
T. Saga ◽  
Y. Tabira ◽  
S. Kitashima ◽  
K. Watanabe ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Staining Technique ◽  
Mental Nerve ◽  
Sihler’S Staining

Communicating branches between lingual and hypoglossal nerve: observation using Sihler’s staining technique

2016 ◽  
Vol 39 (7) ◽  
pp. 741-745 ◽  
Author(s):  
Joe Iwanaga ◽  
Koichi Watanabe ◽  
Tsuyoshi Saga ◽  
Yoko Tabira ◽  
Moriyoshi Nakamura ◽  
...  
Keyword(s):  
Hypoglossal Nerve ◽  
Staining Technique ◽  
Sihler’S Staining ◽  
Communicating Branches

A method for the staining of intraosseous nerve fibers using Sihler’s staining technique

2013 ◽  
Vol 88 (6) ◽  
pp. 290-294 ◽  
Author(s):  
K Shiozaki ◽  
K Miida ◽  
R Tanaka ◽  
S Shimoda
Keyword(s):  
Staining Technique ◽  
Nerve Fibers ◽  
Sihler’S Staining

scholarly journals Mapping the limb muscle motor points for targeted administration of botulinum toxin in the treatment of focal and segmental spasticity

2020 ◽  
Vol 12 (6) ◽  
pp. 61-70
Author(s):  
A. P. Kovalenko ◽  
K. A. Sinelnikov ◽  
V. D. Shamigulov ◽  
N. N. Akhmedov ◽  
E. M. Shamina
Keyword(s):  
Lower Limb ◽  
Staining Technique ◽  
Biological Action ◽  
Lower Limb Muscle ◽  
Limb Muscle ◽  
Anatomical Dissection ◽  
Lower Limb Muscles ◽  
Anatomical Localization ◽  
Sihler’S Staining ◽  
Rehabilitation Measures

Spasticity treatment remains an urgent problem of modern neurorehabilitation. The targeted injection of botulinum neurotoxin (BoNT) into the muscle motor points (MPs) is gaining more and more popularity. But there are insufficient data on the position of MPs, while a MP search methodology has not been worked out yet. Most information about MPs has been obtained on cadaveric material using anatomical dissection or Sihler’s staining technique. Clinical data on the targeted injection of BoNT into the MPs are contradictory, which may be due to the inaccurate determination of their position.Objective: to verify upper and lower limb muscle MPs through electromyography (EMG).Patients and methods. Forty healthy volunteers were examined and underwent anthropometric assessment. Upper and lower limb muscle projections were completely scanned using EMG and ultrasound.Results and discussion. The anatomical localization of MPs in the upper and lower limb muscles involved in spasticity patterns was determined. The position of MPs was found to populationally identical, to have a slight deviation associated with the limb length, and to be unrelated to gender, age, and limb dominance. Original tables and maps for limb MP localization were created.Conclusion. The findings can enhance the efficiency of BoNT administration and improve the quality of rehabilitation measures, since the use of reliable information on the position of MPs will allow targeted BoNT injection in the immediate vicinity of the site of biological action. In clinical practice, this opens a window of opportunity for the early initiation of rehabilitation measures aimed at restoring movement.

Recent Applications of High Resolution Electron Microscopy to Crystalline Arrays of Virus Particles

1978 ◽  
Vol 36 (3) ◽  
pp. 470-482 ◽  
Author(s):  
R.W. Horne
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Analytical Techniques ◽  
Staining Technique ◽  
High Resolution Electron Microscopy ◽  
Optical Diffraction ◽  
Full Potential ◽  
Virus Particles ◽  
Resolution Electron ◽  
Wide Range

The technique of surrounding virus particles with a neutralised electron dense stain was described at the Fourth International Congress on Electron Microscopy, Berlin 1958 (see Home & Brenner, 1960, p. 625). For many years the negative staining technique in one form or another, has been applied to a wide range of biological materials. However, the full potential of the method has only recently been explored following the development and applications of optical diffraction and computer image analytical techniques to electron micrographs (cf. De Hosier & Klug, 1968; Markham 1968; Crowther et al., 1970; Home & Markham, 1973; Klug & Berger, 1974; Crowther & Klug, 1975). These image processing procedures have allowed a more precise and quantitative approach to be made concerning the interpretation, measurement and reconstruction of repeating features in certain biological systems.

The Reaction of Brain Structures with OsO4-Zinc-Iodide Stain

1971 ◽  
Vol 29 ◽  
pp. 272-273
Author(s):  
Werner J. Niklowitz
Keyword(s):  
Electron Microscope ◽  
Structural Changes ◽  
Mossy Fiber ◽  
Toxic Metal ◽  
Staining Technique ◽  
Brain Structures ◽  
Oxidase Inhibitor ◽  
Fiber Layer ◽  
Hippocampal Tissue ◽  
Zinc Iodide

After intoxication of rabbits with certain substances such as convulsant agents (3-acetylpyridine), centrally acting drugs (reserpine), or toxic metal compounds (tetraethyl lead) a significant observation by phase microscope is the loss of contrast of the hippocampal mossy fiber layer. It has been suggested that this alteration, as well as changes seen with the electron microscope in the hippocampal mossy fiber boutons, may be related to a loss of neurotransmitters. The purpose of these experiments was to apply the OsO4-zinc-iodide staining technique to the study of these structural changes since it has been suggested that OsO4-zinc-iodide stain reacts with neurotransmitters (acetylcholine, catecholamines).Domestic New Zealand rabbits (2.5 to 3 kg) were used. Hippocampal tissue was removed from normal and experimental animals treated with 3-acetylpyridine (antimetabolite of nicotinamide), reserpine (anti- hypertensive/tranquilizer), or iproniazid (antidepressant/monamine oxidase inhibitor). After fixation in glutaraldehyde hippocampal tissue was treated with OsO4-zinc-iodide stain and further processed for phase and electron microscope studies.

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