scholarly journals Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

2019 ◽  
Vol 15 (3) ◽  
pp. 765-773 ◽  
Author(s):  
Hou-yong Long ◽  
Qing-xian Huang ◽  
Yong-yang Yu ◽  
Zhen-bin Zhang ◽  
Zeng-wu Yao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Membrane Potential ◽  
Mitochondrial Membrane ◽  
Apoptosis Induction ◽  
Cancer Cell Growth ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest ◽  
Dehydrocostus Lactone

Evaluation of anticancer activity in vitro and in vivo of iridium(iii) polypyridyl complexes

2019 ◽  
Vol 43 (22) ◽  
pp. 8566-8579 ◽  
Author(s):  
Miao He ◽  
Qiao-Yan Yi ◽  
Wen-Yao Zhang ◽  
Lan Bai ◽  
Fan Du ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Membrane Potential ◽  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Cycle Arrest ◽  
Mitochondrial Membrane ◽  
Polypyridyl Complexes ◽  
Cycle Arrest

Three new iridium(iii) polypyridyl complexes were synthesized. The cytotoxic activity in vitro and in vivo, apoptosis, cell cycle arrest, mitochondrial membrane potential, ROS and the expression of Bcl-2 family proteins were investigated.

scholarly journals Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Manman Gu ◽  
Jing Xu ◽  
Chunyang Han ◽  
Youxi Kang ◽  
Tengfei Liu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Dna Damage ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Cell Apoptosis ◽  
Mitochondrial Membrane ◽  
Oxygen Species ◽  
Cycle Arrest ◽  
Murine Fibroblast

Berberine, an isoquinoline alkaloid isolated from several traditional Chinese herbal medicines (TCM), exhibits a strong antimicrobial activity in the treatment of diarrhea. However, it causes human as well as animal toxicity from heavy dosage. The present study was conducted to investigate the cytotoxicity of berberine and its possible trigger mechanisms resulting in cell cycle arrest, DNA damage, ROS (reactive oxygen species) level, mitochondrial membrane potential change, and cell apoptosis in L929 murine fibroblast (L929) cells. The cells were culturedin vitroand treated with different concentrations of berberine for 24 h. The results showed that cell viability was significantly decreased in a subjected dose-dependent state; berberine concentrations were higher than 0.05 mg/mL. Berberine at a concentration above 0.1 mg/mL altered the morphology of L929 cells. Cells at G2/M phase were clear that the level of ROS and cell apoptosis rates increased in 0.1 mg/mL group. Each DNA damage indicator score (DIS) increased in groups where concentration of berberine was above 0.025 mg/mL. The mitochondrial membrane potential counteractive balance mechanics were significantly altered when concentrations of berberine were above 0.005 mg/mL. In all, the present study suggested that berberine at high dosage exhibited cytotoxicity on L929 which was related to resultant: cell cycle arrest; DNA damage; accumulation of intracellular ROS; reduction of mitochondrial membrane potential; and cell apoptosis.

Induction of G1 Cell Cycle Arrest in Human Glioma Cells by Salinomycin Through Triggering ROS-Mediated DNA Damage In Vitro and In Vivo

2016 ◽  
Vol 42 (4) ◽  
pp. 997-1005 ◽  
Author(s):  
Shi-Jun Zhao ◽  
Xian-Jun Wang ◽  
Qing-Jian Wu ◽  
Chao Liu ◽  
Da-Wei Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Cell Cycle Arrest ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

scholarly journals Antitumor effects of iridomyrmecin in HeLa cervical cancer cells are mediated via apoptosis induction, loss of mitochondrial membrane potential, cell cycle arrest and down-regulation of PI3K/Akt and up-regulation of lncRNA CCAT2 expression

2016 ◽  
Vol 11 (4) ◽  
pp. 856
Author(s):  
Li Lin ◽  
Xiao-Ling Cheng ◽  
Ming-Zhe Li ◽  
Tao Wang ◽  
Min-Hai Dong ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Flow Cytometry ◽  
Membrane Potential ◽  
Mitochondrial Membrane ◽  
Apoptosis Induction ◽  
Antitumor Effects ◽  
Western Blot Assay ◽  
Cycle Arrest ◽  
Cervical Cancer Cells

<p class="Abstract">The main purpose of the current study was to study the antitumor effects of iridomyrmecin against human cervical cancer cells (HeLa). Its effects on apoptosis induction, cell cycle phase distribution, PI3K/Akt signalling pathway and long non-coding RNA (lncRNA) were also investigated. Cytotoxic effects of iridomyrmecin were evaluated by MTT assay while the apoptotic effect was assessed by flow cytometry using annexin V-FITC assay. Western blot assay was used to study effects on PI3K/Akt signalling pathway. Results exhibited that iridomyrmecin led to concentration-dependent as well as time-dependent growth inhibitory effects. Iridomyrmecin-treated cells showed signs of early and late apoptosis. Iridomyrmecin treatment also led to sub-G1 cell cycle arrest as well as induced loss of mitochondrial membrane potential (ΔΨm). Further, Western blot assay revealed that iridomyrmecin treatment resulted in down-regulation of PI3K/Akt protein expressions in a dose-dependent manner while as it up-regulated lncRNA CCAT2 expression.</p><p class="Abstract"><strong>Video Clip</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/4mKwQAi3Qbk">Flow cytometry for cell cycle analysis</a>: 3 min 20 sec</p><p> </p>

Cucurbitacin IIb from Ibervillea sonorae Induces Apoptosis and Cell Cycle Arrest via STAT3 Inhibition

2020 ◽  
Vol 20 (10) ◽  
pp. 1188-1196
Author(s):  
Heriberto Torres-Moreno ◽  
Maria C. Marcotullio ◽  
Carlos Velazquez ◽  
Víctor M. Arenas-Luna ◽  
Salomón Hernández-Gutiérrez ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Dna Damage ◽  
Membrane Potential ◽  
Cell Cycle Arrest ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Molecular Mechanisms ◽  
Cyclin B1 ◽  
Cycle Arrest

Background: Cucurbitacin IIb (CIIb) from Ibervillea sonorae has a high capacity to suppress cancer cell proliferation and induce apoptosis. This study investigated the molecular mechanisms related to the antiproliferative and apoptosis induction capacity of CIIb in HeLa cells. Materials and Methods: The cell viability and anti-proliferative effect of CIIb were evaluated by using the trypan blue exclusion assay. The effect of CIIb on the mitochondrial membrane potential was determined by flow cytometry using JC-1. The activity of caspase-3 and caspase-9 was evaluated by flow cytometry using commercial kits. The effect of CIIb on the cell cycle was investigated using Fluorescence-Activated Cell Sorting (FACS) analysis. Western blot analysis was used to evaluate both the inhibitory effect of CIIb on the STAT3 signaling pathway and cyclin –B1, and DNA damage by the comet assay. Results: CIIb triggers disruption of the mitochondrial membrane potential (Δψm) and consequently activated the caspases -3 and -9, as a result of the activation of the intrinsic pathway of the apoptosis. Likewise, the CIIbinduced cell cycle was arrested in S and G2/M after 24h of treatment. CIIb also reduced the expression of STAT3 and cyclin –B1. Finally, CIIb produced an antiproliferative effect at 48 and 72 h, inducing DNA damage. Conclusion: These results demonstrate CIIb-induced apoptosis and cell cycle arrest in HeLa through the inhibition of STAT3.

Copper (I) complexes based on novel N, N′-disubstituted thiocarbamides: Synthesis, spectroscopic, in vitro cytotoxicity, DNA damage and G0/G1 cell cycle arrest studies

2019 ◽  
Vol 491 ◽  
pp. 105-117 ◽  
Author(s):  
Sunil K. Pandey ◽  
Seema Pratap ◽  
Sandeep Pokharia ◽  
Hirdyesh Mishra ◽  
Gaetano Marverti ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Cell Cycle Arrest ◽  
In Vitro Cytotoxicity ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

Cytotoxicity in vitro, cell migration and apoptotic mechanism studies induced by ruthenium(ii) complexes

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 24534-24543 ◽  
Author(s):  
Wei Li ◽  
Bing-Jie Han ◽  
Jun-Hua Yao ◽  
Guang-Bin Jiang ◽  
Yun-Jun Liu
Keyword(s):  
Cell Cycle ◽  
Cell Migration ◽  
Membrane Potential ◽  
Comet Assay ◽  
Cell Cycle Arrest ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Cycle Arrest ◽  
Apoptotic Mechanism

Four Ru(ii) complexes [Ru(N–N)2(DHBT)](ClO4)2 were synthesized and characterized. The cytotoxicity in vitro, apoptosis, comet assay, cell migration, ROS, mitochondrial membrane potential, cell cycle arrest and expression of proteins were investigated.

The induction of apoptosis in BEL-7402 cells through the ROS-mediated mitochondrial pathway by a ruthenium(ii) polypyridyl complex

2014 ◽  
Vol 38 (6) ◽  
pp. 2554-2563 ◽  
Author(s):  
Guang-Bin Jiang ◽  
Jun-Hua Yao ◽  
Ji Wang ◽  
Wei Li ◽  
Bing-Jie Han ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Membrane Potential ◽  
Western Blot ◽  
Cellular Uptake ◽  
Blot Analysis ◽  
Mitochondrial Membrane ◽  
Mitochondrial Pathway ◽  
Cycle Arrest ◽  
Induction Of Apoptosis

The in vitro cytotoxicity, apoptosis, cellular uptake, cell cycle arrest, ROS, mitochondrial membrane potential, western blot analysis and DNA-binding induced by Ru1 were investigated.

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