The induction of apoptosis in BEL-7402 cells through the ROS-mediated mitochondrial pathway by a ruthenium(ii) polypyridyl complex

2014 ◽  
Vol 38 (6) ◽  
pp. 2554-2563 ◽  
Author(s):  
Guang-Bin Jiang ◽  
Jun-Hua Yao ◽  
Ji Wang ◽  
Wei Li ◽  
Bing-Jie Han ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Membrane Potential ◽  
Western Blot ◽  
Cellular Uptake ◽  
Blot Analysis ◽  
Mitochondrial Membrane ◽  
Mitochondrial Pathway ◽  
Cycle Arrest ◽  
Induction Of Apoptosis

The in vitro cytotoxicity, apoptosis, cellular uptake, cell cycle arrest, ROS, mitochondrial membrane potential, western blot analysis and DNA-binding induced by Ru1 were investigated.

Evaluation of anticancer activity in vitro and in vivo of iridium(iii) polypyridyl complexes

2019 ◽  
Vol 43 (22) ◽  
pp. 8566-8579 ◽  
Author(s):  
Miao He ◽  
Qiao-Yan Yi ◽  
Wen-Yao Zhang ◽  
Lan Bai ◽  
Fan Du ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Membrane Potential ◽  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Cycle Arrest ◽  
Mitochondrial Membrane ◽  
Polypyridyl Complexes ◽  
Cycle Arrest

Three new iridium(iii) polypyridyl complexes were synthesized. The cytotoxic activity in vitro and in vivo, apoptosis, cell cycle arrest, mitochondrial membrane potential, ROS and the expression of Bcl-2 family proteins were investigated.

Cytotoxicity In Vitro, Apoptosis, Cellular Uptake, Cell Cycle Distribution, Mitochondrial Membrane Potential Detection, DNA Binding, and Photocleavage of Ruthenium(II) Complexes

2013 ◽  
Vol 66 (5) ◽  
pp. 555 ◽  
Author(s):  
Gan-Jian Lin ◽  
Zheng-Zheng Li ◽  
Jun-Hua Yao ◽  
Hong-Liang Huang ◽  
Yang-Yin Xie ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cellular Uptake ◽  
Mitochondrial Membrane ◽  
Fluorescence Probe ◽  
Membrane Potentials ◽  
Cell Cycle Distribution ◽  
Cycle Arrest ◽  
Pbr322 Dna ◽  
Ethidium Bromide Staining

Four new ruthenium(ii) complexes [Ru(bpy)2(NHPIP)](ClO4)2 (Ru-1), [Ru(phen)2(NHPIP)](ClO4)2 (Ru-2), [Ru(bpy)2(AHPIP)](ClO4)2 (Ru-3), and [Ru(phen)2(AHPIP)](ClO4)2 (Ru-4) (bpy = 2,2′-bipyridine; phen = 1,10-phenanthroline; NHPIP = 2-(3-nitro-4-hydroxylphenyl)imidazo[4,5-f][1,10]phenanthroline; AHPIP = 2-(3-amino-4-hydroxylphenyl)imidazo[4,5-f][1,10]phenanthroline) were synthesized and characterized by elemental analysis, electrospray mass spectrometry, and 1H NMR spectroscopy. The cytotoxicity in vitro of these complexes against BEL-7402, HeLa, MG-63, and MCF-7 cells was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. Ru-4 shows the highest cytotoxic activity towards the selected cell lines among the four complexes. The morphological apoptosis was assayed by an acridine orange/ethidium bromide staining method, and the percentages of necrotic and apoptotic cells were determined by flow cytometry. The cellular uptake and the cell cycle arrest in BEL-7402 cell was investigated. The results showed these complexes inhibit the proliferation of BEL-7402 cells at G0/G1 phase arrest. The detection of mitochondrial membrane potentials using the fluorescence probe JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide) exhibited that the mitochondrial membrane potentials decrease. Upon irradiation, these complexes can effectively cleave pBR322 DNA.

Cytotoxicity in vitro, cell migration and apoptotic mechanism studies induced by ruthenium(ii) complexes

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 24534-24543 ◽  
Author(s):  
Wei Li ◽  
Bing-Jie Han ◽  
Jun-Hua Yao ◽  
Guang-Bin Jiang ◽  
Yun-Jun Liu
Keyword(s):  
Cell Cycle ◽  
Cell Migration ◽  
Membrane Potential ◽  
Comet Assay ◽  
Cell Cycle Arrest ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Cycle Arrest ◽  
Apoptotic Mechanism

Four Ru(ii) complexes [Ru(N–N)2(DHBT)](ClO4)2 were synthesized and characterized. The cytotoxicity in vitro, apoptosis, comet assay, cell migration, ROS, mitochondrial membrane potential, cell cycle arrest and expression of proteins were investigated.

Apoptosis, autophagy, cell cycle arrest, cell invasion and BSA-binding studies in vitro of ruthenium(ii) polypyridyl complexes

RSC Advances ◽  
2016 ◽  
Vol 6 (68) ◽  
pp. 63143-63155 ◽  
Author(s):  
Shang-Hai Lai ◽  
Wei Li ◽  
Xiu-Zhen Wang ◽  
Cheng Zhang ◽  
Chuan-Chuan Zeng ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Comet Assay ◽  
Western Blot ◽  
Cell Invasion ◽  
Blot Analysis ◽  
Binding Studies ◽  
Polypyridyl Complexes ◽  
Cycle Arrest ◽  
Bsa Binding

Four new ruthenium(ii) polypyridyl complexes were synthesized and characterized. The anticancer activity was investigated by cytotoxicity in vitro, apoptosis, comet assay, ROS, autophagy, cell invasion and western blot analysis.

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