scholarly journals Genkwanin Up-Regulates the Transcriptional Activation of Human Type VII Collagen Gene Promoter

2006 ◽  
Vol 40 (4) ◽  
pp. 295-299
Author(s):  
Nozomi Takebayashi ◽  
Miwa Ikeda
Keyword(s):  
Transcriptional Activation ◽  
Gene Promoter ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen

scholarly journals Smad-dependent Transcriptional Activation of Human Type VII Collagen Gene (COL7A1) Promoter by Transforming Growth Factor-β

1998 ◽  
Vol 273 (21) ◽  
pp. 13053-13057 ◽  
Author(s):  
Laurence Vindevoghel ◽  
Atsushi Kon ◽  
Robert J. Lechleider ◽  
Jouni Uitto ◽  
Anita B. Roberts ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transcriptional Activation ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen

Ultraviolet radiation activates human type VII collagen gene (COL7A1) promoter: Involvement of NF-κB

1998 ◽  
Vol 16 ◽  
pp. S218
Author(s):  
Karsten Korang ◽  
Atsushi Kon ◽  
Frank Gasparro ◽  
Jouni Uittlo ◽  
Alain Mauviel
Keyword(s):  
Ultraviolet Radiation ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen

PCR-based detection of two exonic polymorphisms in the human type VII collagen gene (COL7A1) at 3p21.1

Genomics ◽  
1992 ◽  
Vol 14 (3) ◽  
pp. 827-828 ◽  
Author(s):  
Angela M. Christiano ◽  
Linda C. Chung-Honet ◽  
Alain Hovnanian ◽  
Jouni Uitto
Keyword(s):  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen

scholarly journals A GT-rich Sequence Binding the Transcription Factor Sp1 Is Crucial for High Expression of the Human Type VII Collagen Gene (COL7A1) in Fibroblasts and Keratinocytes

1997 ◽  
Vol 272 (15) ◽  
pp. 10196-10204 ◽  
Author(s):  
Laurence Vindevoghel ◽  
Kee-Yang Chung ◽  
Angelique Davis ◽  
David Kouba ◽  
Sirpa Kivirikko ◽  
...  
Keyword(s):  
Transcription Factor ◽  
High Expression ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen ◽  
Transcription Factor Sp1

scholarly journals Transcriptional activation of the minimal human Proα1(I) collagen promoter: obligatory requirement for Sp1

1997 ◽  
Vol 323 (1) ◽  
pp. 225-231 ◽  
Author(s):  
Helen M. POPPLETON ◽  
Rajendra RAGHOW
Keyword(s):  
Binding Site ◽  
Transcriptional Activation ◽  
Gene Promoter ◽  
Tata Box ◽  
Collagen Gene ◽  
Drosophila Line ◽  
Bona Fide ◽  
Necessary And Sufficient ◽  
Collagen Promoter ◽  
Double Stranded Oligonucleotide

A construct containing human Proα1(I) collagen gene promoter/enhancer-driven chloramphenicol acetyltransferase (CAT), pCOL-KT, failed to be expressed significantly in Sp1-deficient Schneider Drosophila line 2 (SL2) cells. However, CAT expression was induced 200-fold in SL2 cells co-transfected with pCOL-KT and pPACSp1, an Sp1-expression vector driven by the Drosophila actin 5C promoter. Elimination of the four potential Sp1-binding sites from pCOL-KT (pCOL-KTΔI), by removal of the first intron, did not abrogate Sp1-mediated induction of CAT. Even more significantly, a minimal Proα1(I) collagen promoter (-100 to +117 bp), containing a TATA box (-28 to -25 bp) and one putative Sp1-binding site (-87 to -82 bp), elicited strong Sp1-induced transactivation. Furthermore, mutation of the Sp1 motif in the minimal Proα1(I) collagen promoter-CAT construct abolished Sp1-induced expression of the reporter gene. Purified Sp1 protein bound specifically to DNA fragments of the Proα1(I) minimal promoter encompassing the putative Sp1-binding site; Sp1 binding could be competed out by a double-stranded oligonucleotide containing the wild-type Sp1 sequence, while an oligonucleotide containing a mutated Sp1 site failed to compete. Based on these results, we postulate that Sp1 plays an obligatory role in the transcriptional activation of the human Proα1(I) collagen gene. Additionally, we propose that a bona fide Sp1 motif, located most proximal to the TATA box, is necessary and sufficient for Sp1-mediated activation of the minimal Proα1(I) collagen promoter.

Structural Organization of the Human Type VII Collagen Gene (COL7A1), Composed of More Exons Than Any Previously Characterized Gene

Genomics ◽  
1994 ◽  
Vol 21 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Angela M. Christiano ◽  
Guy G. Hoffman ◽  
Linda C. Chung-Honet ◽  
Seungbok Lee ◽  
Wen Cheng ◽  
...  
Keyword(s):  
Structural Organization ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen

Complete characterization of the human type VII collagen gene

1993 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Jouni Uitto ◽  
Angela M. Christiano ◽  
Linda C. Chung-Honet ◽  
Daniel S. Greenspan
Keyword(s):  
Complete Characterization ◽  
Collagen Gene ◽  
Human Type ◽  
Type Vii Collagen
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