scholarly journals Comparison of Broth Dilution and Disc Diffusion Method for the Antifungal Susceptibility Testing of Aspergillus flavus

2010 ◽  
pp. 202-208 ◽  
Author(s):  
Ravi Kumar ◽  
Sandeep Kumar Shrivastava ◽  
Arunaaloke Chakraborti
Keyword(s):  
Aspergillus Flavus ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Broth Dilution

scholarly journals Comparison of antiaspergillar activity of extracts of Tectona grandis Linn according to two antifungal susceptibility testing

2016 ◽  
Vol 5 (3) ◽  
pp. 93-99
Author(s):  
Elisée Kporou Kouassi ◽  
◽  
Ibourahema Coulibaly ◽  
Gervais Melaine M'boh ◽  
Sitapha Ouattara ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Folk Medicine ◽  
Tectona Grandis ◽  
Antifungal Susceptibility Testing ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Agar Slant ◽  
Broth Dilution

Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regularly evaluated in different Laboratories. And, the processes of evaluation of antifungal susceptibility are often different to one laboratory to another. This study was undertaken in the goal to compare parameters obtained (MIC and MFC) with two antifungal susceptibility testing. The first one is Agar slant double dilution tubes method (M1) and the second is disc diffusion method /broth dilution according document CLSI M38 -A2 supplemented to 2% glucose (M2). Ethanol (EtOH 70%), Methanol (MeOH) and n-Hexane (n-HexTG) extracts of leaves of Tectona grandis have been tested in comparison to Itraconazole against two clinicals strains of Aspergillus fumigatus 896/AB and Aspergillus flavus 1006/OM isolated to HIV patients. Results showed that on A. flavus, agar slant method (M1) gave MIC ranging between 200 µg/mL and 800 µg/mL while disc diffusion meth/broth dilution (M2) showed growth inhibition between 100 µg/mL and 400 µg/mL. On A. fumigatus, inhibition was observed between 200µg/mL and 400 µg/mL with M2 in contrary M1 gave MIC located between 400 µg/mL and 800 µg/mL. For each strain tested, according method M2, MIC and MFC are identical and with method M1, these parameters were different. Evaluation of Itraconazole on each strain gave with M2, MIC= 50 µg/mL while inhibition has been showed at 100 µg/mL and 200µg/mL with M1. Also considering extracts, hydroalcoholic extract of T. grandis (EtOHTG) showed antifungal susceptibility testing less 2 to 4 efficiency than Itraconazole. Disc diffusion method/broth dilution (M2) according CLSI M38-A2 to evaluate Tectona grandis antifungal susceptibility is the process of evaluation which allo wed having lo west antifungal parameters (MIC and MFC) on A. flavus and A. fumigatus.

scholarly journals Russia-made Mueller–Hinton agar: compliance with contemporary requirements

2019 ◽  
Vol 9 (2) ◽  
pp. 409-416
Author(s):  
L. V. Domotenko ◽  
I. S. Kosilova ◽  
A. P. Shepelin
Keyword(s):  
Quality Control ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Nutrient Media ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

At present, a rise of antimicrobial resistance requires that susceptibility of infectious agents to antimicrobial agents could be accurately evaluated as related errors may lead to selecting improper therapeutics provoking spread of drug resistance. Pathogen sensitivity to antimicrobial agents is commonly determined by a disc diffusion method. A quality of nutrient medium used in assays plays a crucial role influencing final results. In Russia, it turned out that regulatory documents such as the nationwide guidelines and clinical recommendations outlining methodology for antimicrobial susceptibility testing underlay availability in domestic market few nutrient media, including Mueller–Hinton Agar, AGV medium etc. exhibiting sometimes unsatisfactory quality. To harmonize such methodology with international requirements, theStateResearchCenterfor Applied Microbiology and Biotechnology has developed a technology and promoted manufacture of Russia-made Mueller–Hinton agar satisfying requirements of EUCAST documents, clinical guidelines, and ISO/TS 16782:2016. The main objective of this study was to compare quality of new agar product with five similar foreign media while examining 11 test strains by disc diffusion method. As a result, some of nutrient media available to the Russian market turned out to be off-standard: not all of them satisfy to the EUCAST requirements and clinical guidelines since diameter distribution for growth inhibition recommended by EUCAST for quality control does not fit into permissible range. Moreover, susceptibility of P. aeruginosa ATCC 27853 to aminoglycosides, fluoroquinolones, Meropenem, as well as S. aureus ATSS 25923 and E. faecalis ATCC 29212 to tigecycline was assessed with certain mistakes. The data obtained by us were analyzed in accordance to the new document ISO/TS 16782:2016 “Clinical laboratory testing — criterion for acceptable lots of dehydrated Mueller–Hinton agar and broth for antimicrobial susceptibility testing”, not approved yet In Russia. To determine potential reason for deviation of data from reference range, we measured concentration of bivalent metals in all nutrient media examined by atomic emission spectrometry with inductively coupled plasma. We determined new patterns affecting reliability of results on microbial antibiotic susceptibility. A need to check intralaboratory quality control of nutrient media was emphasized.  

scholarly journals Comparing Etest and Broth Microdilution for Antifungal Susceptibility Testing of the Most-Relevant Pathogenic Molds

2015 ◽  
Vol 53 (10) ◽  
pp. 3176-3181 ◽  
Author(s):  
Frédéric Lamoth ◽  
Barbara D. Alexander
Keyword(s):  
Amphotericin B ◽  
Antifungal Therapy ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Multidrug Resistant ◽  
Antifungal Susceptibility Testing ◽  
Diffusion Method ◽  
Broth Microdilution ◽  
Content Type ◽  
Broth Microdilution Method

Invasive mold infections are life-threatening diseases for which appropriate antifungal therapy is crucial. Their epidemiology is evolving, with the emergence of triazole-resistantAspergillusspp. and multidrug-resistant non-Aspergillusmolds. Despite the lack of interpretive criteria, antifungal susceptibility testing of molds may be useful in guiding antifungal therapy. The standard broth microdilution method (BMD) is demanding and requires expertise. We assessed the performance of a commercialized gradient diffusion method (Etest method) as an alternative to BMD. The MICs or minimal effective concentrations (MECs) of amphotericin B, voriconazole, posaconazole, caspofungin, and micafungin were assessed for 290 clinical isolates of the most representative pathogenic molds (154Aspergillusand 136 non-Aspergillusisolates) with the BMD and Etest methods. Essential agreements (EAs) within ±2 dilutions of ≥90% between the two methods were considered acceptable. EAs for amphotericin B and voriconazole were >90% for most potentially susceptible species. For posaconazole, the correlation was acceptable forMucoromycotinabut Etest MIC values were consistently lower forAspergillusspp. (EAs of <90%). Excellent EAs were found for echinocandins with highly susceptible (MECs of <0.015 μg/ml) or intrinsically resistant (MECs of >16 μg/ml) strains. However, MEC determinations lacked consistency between methods for strains exhibiting mid-range MECs for echinocandins. We concluded that the Etest method is an appropriate alternative to BMD for antifungal susceptibility testing of molds under specific circumstances, including testing with amphotericin B or triazoles for non-Aspergillusmolds (MucoromycotinaandFusariumspp.). Additional study of molecularly characterized triazole-resistantAspergillusisolates is required to confirm the ability of the Etest method to detect voriconazole and posaconazole resistance amongAspergillusspp.

scholarly journals Antimicrobial Activity of Cymbopogon (Lemongrass) on Enterococci

2021 ◽  
pp. 684-690
Author(s):  
A. Sai Sanjith ◽  
N. P. Muralidharan
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Lemongrass Oil ◽  
Test Organisms ◽  
Broth Dilution

Aim: The aim of this study was to investigate antimicrobial activity against Enterococcus bacteria. Materials and Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Broth Dilution Method. The antibiotic susceptibility test against the test organisms was performed by the Disc Diffusion Method. Results and Discussion: Antimicrobial activity of Cymbopogon oil was examined against common Enterococcus bacteria using broth microdilution assay and the disc diffusion method. Zone of inhibition was seen when Cymbopogon oil was kept with enterococcus bacteria, this proves to be bacteriostatic. Conclusion: Lemongrass oil proved to be bacteriostatic against Enterococcus, further research to be done to implement Lemongrass oil as an antibacterial agent.

scholarly journals In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis

2020 ◽  
Vol 25 (1) ◽  
Author(s):  
Mohamed T. Yassin ◽  
Ashraf A. Mostafa ◽  
Abdulaziz A. Al-Askar ◽  
Rashad Bdeer
Keyword(s):  
Antifungal Agents ◽  
Health Hazard ◽  
Antifungal Susceptibility ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Its Sequencing ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Resistance Profile

Abstract Background Vulvovaginal candidiasis (VVC) represents a universal health hazard that contributes to significant morbidity in women. Resistance of Candida to antifungal therapy has been reported as a public health problem. So, the objective of our current study is to detect resistance profile of different candidal strains. Methods In this study, isolated Candida strains were identified by conventional methods, confirmed by internal transcribed spacer (ITS) sequencing, and phylogenetically analyzed with reference strains in GenBank. Also, sensitivity of different Candida strains to common antifungal agents was evaluated by disc diffusion method. Results Candida albicans was identified as the most frequent strain (63%) followed by non-albicans strains, such as C. glabrata (20%), C. tropicalis (13%), and C. krusei (4%). Sensitivity of Candida strains (C. albicans, C. tropicalis and C. glabrata) to commonly used antifungal agents was evaluated through the disc diffusion method. C. glabrata was the most resistant strain and considered to be a multidrug-resistant pathogen, while both, C. albicans and C. tropicalis showed high susceptibility to terbinafine. In contrast, C. albicans showed resistance to fluconazole, clotrimazole, and nystatin, while C. tropicalis, considered as the most sensitive strain, was susceptible to all the antifungal agents tested except nystatin. Terbinafine was the most effective antifungal agent against both C. tropicalis and C. albicans, and hence its minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for C. albicans and C. tropicalis were evaluated. MICs of terbinafine against C. albicans and C. tropicalis were 5 μg/ml and 2.5 μg/ml, while their MFCs were 10 μg/ml and 5 μg/ml, respectively. Conclusion The emergence of resistant Candida strains necessitates conduction of the antifungal susceptibility test prior to deciding the medication regime.

scholarly journals Stenotrophomonas maltophilia endogenous endophthalmitis: clinical presentation, sensitivity spectrum and management

2009 ◽  
Vol 58 (6) ◽  
pp. 837-838 ◽  
Author(s):  
T. Das ◽  
H. S. Deshmukh ◽  
A. Mathai ◽  
Ashok Kumar Reddy
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Susceptibility Testing ◽  
Clinical Presentation ◽  
Diffusion Method ◽  
Endogenous Endophthalmitis ◽  
Antibiotic Susceptibility Testing ◽  
Disc Diffusion ◽  
Antibiotic Regimen ◽  
Nosocomial Pathogen ◽  
Disc Diffusion Method

Stenotrophomonas maltophilia is gaining importance as a community-acquired pathogen, after becoming firmly established as a nosocomial pathogen. Here we report a case of endogenous endophthalmitis due to S. maltophilia. Antibiotic-susceptibility testing of the isolate was performed by the Kirby–Bauer disc diffusion method. The organism was resistant to aminoglycosides, imipenem, ticarcillin and cotrimoxazole and was sensitive to ceftazidime and chloramphenicol. The patient was successfully treated with a sensitivity-based intravitreal antibiotic regimen.

Sign in / Sign up

Export Citation Format

Share Document