scholarly journals Effect of plant growth regulators and carbon source on the shoot regeneration and rooting of ‘Wonhwang’ pear (Pyrus pyrifoliaL.)

2016 ◽  
Vol 43 (4) ◽  
pp. 486-491 ◽  
Author(s):  
Se Hee Kim ◽  
Seo Jun Park ◽  
Kang Hee Cho ◽  
Han Chan Lee
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators

Effect of Plant Growth Regulators in Shoot Regeneration of Phaleria Macrocarpa in Vitro

2018 ◽  
Vol 5 (1) ◽  
pp. 29-33
Author(s):  
Rosilah Ab Aziz ◽  
Nur Atiqah Jamari ◽  
Izzatul Zuliana Zolkefli
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Phaleria Macrocarpa

scholarly journals Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro

Plant Root ◽  
2013 ◽  
Vol 7 (0) ◽  
pp. 5-11 ◽  
Author(s):  
Hideyuki Tanaka ◽  
Masahumi Johkan ◽  
Kazuhiko Mitsukuri ◽  
Takahiro Tezuka ◽  
Hajime Furukawa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Intact Roots

scholarly journals Optimization of Protoplast Isolation and Micropropagation Techniques in Rose

2020 ◽  
Vol 40 (01) ◽  
Author(s):  
Anwesha Ash ◽  
Geetanjali , K.V. Rashmi Chakraborty ◽  
K. V. Rashmi
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Protoplast Isolation ◽  
Hormone Concentration ◽  
Meristematic Tissue ◽  
Important Species ◽  
Irregular Pattern ◽  
Aesthetic Values

Rose is the dominant flower in the ornamental industry because of its multiple aesthetic values. Optimization of plant growth regulators in media was achieved for one of the important species of rose. The nodal explants were tested on a full strength MS media with different hormone concentrations of 6-benzylaminopurine (BAP) and Kinetin (Kn). Maximum response was obtained for a combined hormone concentration of 6-benzylaminopurine (3.5mg/L) and kinetin (1.5mg/L) with shoot regeneration from nodes, internodes and apical meristematic tissue. 6-benzylaminopurine alone showed shoot regeneration only from internodes and nodes, while kinetin alone showed shoot regeneration only from apical meristematic tissue. Protoplast of Rosa sp. and Rosa sinensis were isolated enzymatically and fused using polyethylene. The protoplasts were observed to fuse on different planes in an irregular pattern.

scholarly journals Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex F.H. Wigg

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1181
Author(s):  
María Eugenia Martinez ◽  
Lorena Jorquera ◽  
Paola Poirrier ◽  
Katy Díaz ◽  
Rolando Chamy
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Callus Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Large Scale ◽  
Taraxacum Officinale ◽  
Naphthaleneacetic Acid

Taraxacum officinale (L.) Weber ex F.H. Wigg, commonly known as dandelion, is a cosmopolitan and perennial weed, which has medicinal properties. In vitro propagation methods are widely used on plants that have difficulties in cultivation and, consequently, low extraction yields of active metabolites. Thus, callus culture has been considered to be useful for the accumulation of several metabolites. In this study, we aimed to establish an efficient protocol for callus induction and maintenance of T. officinale, for which explant type, carbon source, light conditions, and nine different combinations of plant growth regulators (PGRs), such as 1-naphthaleneacetic acid (NAA) (from 0.05 to 0.5 mg/L) and 6-benzylaminopurine acid (BAP) (from 0.5 to 3.0 mg/L), were evaluated. The results showed that hypocotyls and roots from sterile seedlings are the best sources for callus induction, with 100% of callogenesis at every condition tested, and more than 95% of viability and friability. Complete darkness and a medium supplemented with sucrose at 2.3% (w/v) and 0.5 mg/L of NAA and 0.5 mg/L of BAP were the best conditions for callus induction, showing callus with low organogenesis and high friability. This study provides a basis for future studies on improving large-scale callus propagation and further establishment of suspension culture systems for commercial purposes.

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