Effect of Plant Growth Regulators in Shoot Regeneration of Phaleria Macrocarpa in Vitro

2018 ◽  
Vol 5 (1) ◽  
pp. 29-33
Author(s):  
Rosilah Ab Aziz ◽  
Nur Atiqah Jamari ◽  
Izzatul Zuliana Zolkefli
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Phaleria Macrocarpa

scholarly journals Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro

Plant Root ◽  
2013 ◽  
Vol 7 (0) ◽  
pp. 5-11 ◽  
Author(s):  
Hideyuki Tanaka ◽  
Masahumi Johkan ◽  
Kazuhiko Mitsukuri ◽  
Takahiro Tezuka ◽  
Hajime Furukawa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Intact Roots

scholarly journals Effect of explant source and growth regulators on in vitro callus induction and organogenesis of Melia azedarach L. trees

2014 ◽  
Vol 8 (3) ◽  
pp. 20-27
Author(s):  
Siham Abd Al-Razzaq Salim
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Adventitious Shoots ◽  
Melia Azedarach ◽  
Peat Moss ◽  
Naphthalene Acetic Acid

Melia azedarach L. is one of the important plants because it’s a good source of natural compounds thathave insecticide and antimicrobial effect. The main aim of this research is to investigate the effect of explants source and plant growth regulators on in vitro callus induction and regeneration of organs from it. Callus was induced from nodes, internodes from one-year-old seedlings and seeds of Melia plant by culturing them on MS medium supplemented with α-naphthalene acetic acid (NAA) 0.0, 0.1, 0.2, 0.3, or 0.4 mg/L and 6- benzyl adenine (BA) 0.0, 1.0, 2.0, 3.0, or 4.0 mg/L, then shoot regeneration from callus was occurred. Results showed that there was a different response from explants towards callus induction and adventitious shoots formation according to plant growth regulators combination. Seeds gave superior percentage for callus induction 24.4% compared with node and internode 15.6, 12.8% respectively. Combination of 0.3 mg/L NAA + 3.0 mg/L BA was the best for callus induction in all explants 86.6% . Shoot regeneration was achieved in 0.3 mg/L NAA + 4.0 mg/L BA and 0.4 mg/L NAA + 4.0 mg/L BA for callus from seeds and internodes respectively, while the combination 0.3 mg/L NAA + 3.0 mg/L BA or 0.4 mg/L NAA + 3.0 mg/L BA was the best for node callus. The shoots were rooted well in MS + 0.25 mg/L NAA . Rooted plantlets were acclimatized in small plastic pots filled with peat moss: river soil (1 :1 v/v ), then transferred to the soil.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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