scholarly journals Factors of Endoscopic Ultrasound-Guided Tissue Acquisition for Successful Next-Generation Sequencing in Pancreatic Ductal Adenocarcinoma

Gut and Liver ◽  
2020 ◽  
Vol 14 (3) ◽  
pp. 387-394 ◽  
Author(s):  
Jae Keun Park ◽  
Ji Hyeon Lee ◽  
Dong Hyo Noh ◽  
Joo Kyung Park ◽  
Kyu Taek Lee ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Endoscopic Ultrasound ◽  
Ductal Adenocarcinoma ◽  
Next Generation ◽  
Ultrasound Guided ◽  
Tissue Acquisition ◽  
Generation Sequencing

scholarly journals New Era of Endoscopic Ultrasound-Guided Tissue Acquisition: Next-Generation Sequencing by Endoscopic Ultrasound-Guided Sampling for Pancreatic Cancer

2019 ◽  
Vol 8 (8) ◽  
pp. 1173 ◽  
Author(s):  
Imaoka ◽  
Sasaki ◽  
Hashimoto ◽  
Watanabe ◽  
Ikeda
Keyword(s):  
Pancreatic Cancer ◽  
Next Generation Sequencing ◽  
Endoscopic Ultrasound ◽  
Next Generation ◽  
Ultrasound Guided ◽  
Tissue Samples ◽  
Genomic Biomarkers ◽  
Tissue Acquisition ◽  
Chemotherapy Patients ◽  
Generation Sequencing

Pancreatic cancer is a lethal cancer with an increasing incidence. Despite improvements in chemotherapy, patients with pancreatic cancer continue to face poor prognoses. Endoscopic ultrasound-guided tissue acquisition (EUS-TA) is the primary method for obtaining tissue samples of pancreatic cancer. Due to advancements in next-generation sequencing (NGS) technologies, multiple parallel sequencing can be applied to EUS-TA samples. Genomic biomarkers for therapeutic stratification in pancreatic cancer are still lacking, however, NGS can unveil potential predictive genomic biomarkers of treatment response. Thus, the importance of NGS using EUS-TA samples is becoming recognized. In this review, we discuss the recent advances in EUS-TA application for NGS of pancreatic cancer.

scholarly journals Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Imteyaz Ahmad Khan ◽  
Safoora Rashid ◽  
Nidhi Singh ◽  
Sumaira Rashid ◽  
Vishwajeet Singh ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Early Stage ◽  
Ductal Adenocarcinoma ◽  
Next Generation ◽  
Serum Samples ◽  
Tissue Samples ◽  
Non Invasive ◽  
Specific Symptoms ◽  
Generation Sequencing

AbstractEarly-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.

Utility of Endoscopic Ultrasound-Guided Biopsy for Next-Generation Sequencing of Pancreatic Exocrine Malignancies

Pancreas ◽  
2018 ◽  
Vol 47 (8) ◽  
pp. 990-995 ◽  
Author(s):  
Brent K. Larson ◽  
Richard Tuli ◽  
Laith H. Jamil ◽  
Simon K. Lo ◽  
Nan Deng ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Endoscopic Ultrasound ◽  
Next Generation ◽  
Ultrasound Guided ◽  
Guided Biopsy ◽  
Pancreatic Exocrine ◽  
Generation Sequencing ◽  
Ultrasound Guided Biopsy

Endoscopic Ultrasound Tissue Acquisition for Genomic Analysis of Pancreatic Cancer Using Liquid Next-Generation Sequencing

2018 ◽  
Vol 113 (Supplement) ◽  
pp. S13
Author(s):  
Nadim Mahmud ◽  
Sherif Elhanafi ◽  
Michael L. Kochman ◽  
Gregory Ginsberg ◽  
Norge Vergara ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Next Generation Sequencing ◽  
Endoscopic Ultrasound ◽  
Genomic Analysis ◽  
Next Generation ◽  
Tissue Acquisition ◽  
Generation Sequencing

Next‐generation sequencing of endoscopic ultrasound guided microbiopsies from pancreatic cystic neoplasms

2019 ◽  
Vol 75 (5) ◽  
pp. 767-771 ◽  
Author(s):  
Charlotte Vestrup Rift ◽  
Linea C Melchior ◽  
Bojan Kovacevic ◽  
Anders Toxværd ◽  
Pia Klausen ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Endoscopic Ultrasound ◽  
Next Generation ◽  
Ultrasound Guided ◽  
Cystic Neoplasms ◽  
Pancreatic Cystic Neoplasms ◽  
Generation Sequencing
Sign in / Sign up

Export Citation Format

Share Document