Removal efficiency and mechanisms of antibiotic resistance genes in secondary effluent by combined process of coagulation-sedimentation-ultrafiltration

2020 ◽  
Vol 190 ◽  
pp. 80-88
Author(s):  
Lihua Sun ◽  
Yu Ding ◽  
Qiwei Zhang ◽  
Ning He ◽  
Cuimin Feng
Keyword(s):  
Antibiotic Resistance ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Secondary Effluent ◽  
Combined Process

scholarly journals Removal of antibiotic resistance genes in secondary effluent by slow filtration-NF process

2021 ◽  
Author(s):  
Lihua Sun ◽  
Yao Ma ◽  
Yu Ding ◽  
Xiaoyu Mei ◽  
Yehui Liu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Population Structure ◽  
Resistance Genes ◽  
Filtration Rate ◽  
Microbial Population ◽  
Antibiotic Resistance Genes ◽  
Secondary Effluent ◽  
Combined Process ◽  
Optimal Filtration ◽  
Slow Filtration

Abstract In this study, the combined process of slow filtration and low pressure nanofiltration (NF) has been used to deeply remove the antibiotic resistance genes (ARGs) in a secondary effluent, and the mechanism of ARGs removal has been subsequently explored. It is observed that the optimal filtration rate for the slow filtration without biofilm, slow filtration with the aerobic heterotrophic biofilm, slow filtration with the nitrification biofilm and slow filtration with the denitrification biofilm to remove tet A, tet W, sul I, sul II and DOC is 20 cm/h, and the slow filtration with the aerobic heterotrophic biofilm exhibits the highest removal amount. The slow filtration with biofilms removes a high extent of free ARGs. As compared with the direct NF of the secondary effluent and the slow filtration without biofilm-NF, the slow filtration with the aerobic heterotrophic biofilm-NF combined process exhibits the best ARGs removal effect. The microbial population structure and the high filtration rate in the aerobic heterotrophic biofilmand promote the removal of ARGs. Strengthening the removal of 16S rDNA, intI 1 and DOC can improve the ARGs removal effect of the combined process. Overall, the slow filtration-NF combined process is a better process for removing ARGs.

Removal of bacterial cells, antibiotic resistance genes and integrase genes by on-site hospital wastewater treatment plants: surveillance of treated hospital effluent quality

2017 ◽  
Vol 3 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Kenda Timraz ◽  
Yanghui Xiong ◽  
Hamed Al Qarni ◽  
Pei-Ying Hong
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Bacterial Cells ◽  
Hospital Wastewater ◽  
Effluent Quality ◽  
Microbial Contaminants

This study aims to evaluate the removal efficiency of microbial contaminants by wastewater treatment plants (WWTPs) operated on-site of two hospitals.

scholarly journals Removal of antibiotic resistance genes from secondary effluent by processes combining nano-iron, ultrasound-activated persulfate, and ultrafiltration

2021 ◽  
Author(s):  
Lihua Sun ◽  
Hao Tong ◽  
Cheng Gao ◽  
Yehui Liu ◽  
Cuimin Feng
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Sewage Treatment ◽  
Antibiotic Resistance Genes ◽  
Oxidation Mechanism ◽  
Environmental Pollutant ◽  
Secondary Effluent ◽  
Class 1 Integron ◽  
Class 1 ◽  
Activated Persulfate

Abstract Antibiotic resistance genes (ARGs), as a new type of environmental pollutant which threaten human health, have been detected in the effluent of sewage treatment systems. In this study, the removal from water of ARGs, 16S rRNA, class 1 integron (intI1), and dissolved organic carbon (DOC) were investigated using processes combining nano-iron (nFe), ultrasound (US), activated persulfate (PS) and ultrafiltration (UF). The oxidation mechanism was also studied. The results showed that both nFe and US activation could improve the oxidative effect of PS, and the effect of nFe was better than that of US. Compared with PS-UF, nFe/PS-UF and US/PS-UF significantly enhanced the removal of various ARGs and DOC. nFe/PS-UF was the most effective treatment, reducing cell-associated and cell-free ARGs by 1.74–3.14-log and 1.00–2.61-log, respectively, while removing 30% of DOC. Pre-oxidation methods using PS, nFe/PS, and US/PS significantly enhanced the efficacy of UF for removing DOC with molecular weights above 50 kDa and below 10 kDa, but the removal of DOC between 10 and 50 kDa decreased. The free radicals SO4·− and ·OH were shown to participate in the process of ARGs oxidation.

scholarly journals Removal of Antibiotic Resistance Genes at Two Conventional Wastewater Treatment Plants of Louisiana, USA

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1729 ◽  
Author(s):  
Ocean Thakali ◽  
John P. Brooks ◽  
Shalina Shahin ◽  
Samendra P. Sherchan ◽  
Eiji Haramoto
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
Antibiotic Resistance Genes ◽  
Resistance Mechanisms ◽  
Secondary Effluent ◽  
Integrase Gene ◽  
Final Effluent

Wastewater treatment plants (WWTPs) represent all known types of antibiotic resistance mechanisms and are considered as the critical points for the spread of antibiotic resistance genes (ARGs). The purpose of this study is to investigate the removal of a Class 1 integrase gene (intI1) and a selected set of ARGs (blaTEM, ermF, mecA, and tetA) at two conventional WWTPs by using chlorination in Louisiana, USA. We collected 69 wastewater samples (23 each from influent, secondary effluent, and final effluent) and determined the concentrations of ARGs by using quantitative polymerase chain reaction. All tested ARGs, except for mecA, were detected in 83–96% and 30–65% of influent and final effluent samples, respectively. Although the ARGs underwent approximately 3-log10 reduction, two WWTPs on an average still released 3.3 ± 1.7 log10 copies/mL of total ARGs studied in the effluents. Chlorination was found to be critical in the significant reduction of total ARGs (p < 0.05). Correlation analysis and the ability of intI1 to persist through the treatment processes recommend the use of intI1 as a marker of ARGs in effluents to monitor the spread of antibiotic resistance in effluents. Our study suggests that conventional WWTPs using chlorination do not favor the proliferation of antibiotic resistance bacteria and ARGs during wastewater treatment.

scholarly journals Pollution Characteristics of Typical Antibiotic Resistance Genes in Sludge Treatment Wetlands

2020 ◽  
Vol 194 ◽  
pp. 04010
Author(s):  
Zhuma Luosang ◽  
Wanjun Zhang ◽  
Junwen Ma ◽  
fengying Huang ◽  
Yubo Cui
Keyword(s):  
Antibiotic Resistance ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Bottom Layer ◽  
Treatment Wetlands ◽  
Sludge Treatment ◽  
Pollution Characteristics ◽  
Box Method

The in-situ static box method was used to conduct a comparative experiment on sludge treatment wetlands (STWs). STW1 had ventilation structure, without reeds and STW2 had ventilation and was planted with reeds. The absolute abundance of sulfonamide, tetracycline and macrolide antibiotic resistance genes (ARGs) in the two STWs were analyzed, and the paper discussed pollution characteristics of typical antibiotic resistance genes in the two STWs. The results showed that three ARGs, sul1 (sulfonamides), tetC (tetracyclines), ermf (macrolides), were detected in STW1 and STW2. The concentration of arginine was sul1 > tetC > ermf. The concentration level of AGRS in STW1 and STW2 was lower in bottom layer than that of surface layer sludge. The removal efficiency of ARGs in the same system was tetC > sul1 > ermf, and the removal efficiency of surface sludge and bottom sludge in different systems was STW2 > STW1. Planting wetland plants in the STW can promote the removal of ARGs.

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