Removal of bacterial cells, antibiotic resistance genes and integrase genes by on-site hospital wastewater treatment plants: surveillance of treated hospital effluent quality

2017 ◽  
Vol 3 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Kenda Timraz ◽  
Yanghui Xiong ◽  
Hamed Al Qarni ◽  
Pei-Ying Hong
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Bacterial Cells ◽  
Hospital Wastewater ◽  
Effluent Quality ◽  
Microbial Contaminants

This study aims to evaluate the removal efficiency of microbial contaminants by wastewater treatment plants (WWTPs) operated on-site of two hospitals.

scholarly journals Antibiotic Resistance Genes Dynamics at the Different Stages of the Biological Process in a Full-Scale Wastewater Treatment Plant

Proceedings ◽  
2018 ◽  
Vol 2 (11) ◽  
pp. 650 ◽  
Author(s):  
Ioanna Zerva ◽  
Ioanna Alexandropoulou ◽  
Maria Panopoulou ◽  
Paraschos Melidis ◽  
Spyridon Ntougias
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plant ◽  
Biological Process ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Antibiotic Resistance Genes ◽  
Additional Treatment ◽  
Full Scale

Wastewater treatment plants (WWTPs) highly contribute to the transmission of antibiotic resistance genes (ARGs) in the environment. In this work, the diversity of ermF, ermB, sul1 and int1-enconding genes was examined in the influent, the mixed liquor and the effluent of a full-scale WWTP. Based on the clones analyzed, similar genotypes were recorded at all process stages. However, distinct genotypes of int1 were responsible for the expression of sul1 and ermF genes in Gammaproteobacteria and Bacteroidetes, respectively. Due to the detection of similar ARGs profiles throughout the biological process, it is concluded that additional treatment is needed for their retention.

scholarly journals Metagenomic Profiles of Antibiotic Resistance Genes in Activated Sludge, Dewatered Sludge and Bioaerosols

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1516
Author(s):  
Il Han ◽  
Keunje Yoo
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Illumina Hiseq ◽  
Wastewater Treatment Process ◽  
Dewatered Sludge ◽  
Scientific Attention

Wastewater treatment plants (WWTPs) have been considered hotspots for the development and dissemination of antibiotic resistance in the environment. Although researchers have reported a significant increase in bioaerosols in WWTPs, the associated bacterial taxa, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) remain relatively unknown. In this study, we have investigated the abundance and occurrences of ARGs and MGEs, as well as the bacterial community compositions in activated sludge (AS), dewatered sludge (DS) and bioaerosols (BA) in a WWTP. In total, 153 ARG subtypes belonging to 19 ARG types were identified by the broad scanning of metagenomic profiles obtained using Illumina HiSeq. The results indicated that the total occurrences and abundances of ARGs in AS and DS samples were significantly higher than those in BA samples (p < 0.05). However, some specific ARG types related to sulfonamide, tetracycline, macrolide resistance were present in relatively high abundance in BA samples. Similar to many other full-scale WWTPs, the Proteobacteria (58%) and Bacteroidetes (18%) phyla were dominant in the AS and DS samples, while the Firmicutes (25%) and Actinobacteria (20%) phyla were the most dominant in the BA samples. Although the abundance of genes related to plasmids and integrons in bioaerosols were two to five times less than those in AS and DS samples, different types of MGEs were observed in BA samples. These results suggest that comprehensive analyses of resistomes in BA are required to better understand the emergence of both ARGs and MGEs in the wastewater treatment process due to the significant increase of scientific attention toward bioaerosols effects.

Sign in / Sign up

Export Citation Format

Share Document