Evaluating the allelochemical effects of Syzygium cumini leaves extract on Microcystis aeruginosa by pulse-amplitude-modulated fluorometry and flow cytometry

2018 ◽  
Vol 133 ◽  
pp. 220-226
Author(s):  
Xiao-xiong Wang ◽  
Zhi-hao Xiao ◽  
Cheng-chun Jiang ◽  
Xiao-jun Wang ◽  
Yim-tong Szeto
Keyword(s):  
Flow Cytometry ◽  
Microcystis Aeruginosa ◽  
Pulse Amplitude ◽  
Syzygium Cumini ◽  
Allelochemical Effects

scholarly journals Effect of Zinc on Microcystis aeruginosa UTEX LB 2385 and Its Toxin Production

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 92 ◽  
Author(s):  
Jose L. Perez ◽  
Tinchun Chu
Keyword(s):  
Flow Cytometry ◽  
Stress Response ◽  
Microcystis Aeruginosa ◽  
Stress Responses ◽  
Algal Blooms ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Zinc Stress ◽  
Cell Quota ◽  
Contrast Microscopy

Cyanobacteria harmful algal blooms (CHABs) are primarily caused by man-made eutrophication and increasing climate-change conditions. The presence of heavy metal runoff in affected water systems may result in CHABs alteration to their ecological interactions. Certain CHABs produce by-products, such as microcystin (MC) cyanotoxins, that have detrimentally affected humans through contact via recreation activities within implicated water bodies, directly drinking contaminated water, ingesting biomagnified cyanotoxins in seafood, and/or contact through miscellaneous water treatment. Metallothionein (MT) is a small, metal-sequestration cysteine rich protein often upregulated within the stress response mechanism. This study focused on zinc metal resistance and stress response in a toxigenic cyanobacterium, Microcystis aeruginosa UTEX LB 2385, by monitoring cells with (0, 0.1, 0.25, and 0.5 mg/L) ZnCl2 treatment. Flow cytometry and phase contrast microscopy were used to evaluate physiological responses in cultures. Molecular assays and an immunosorbent assay were used to characterize the expression of MT and MC under zinc stress. The results showed that the half maximal inhibitory concentration (IC50) was 0.25 mg/L ZnCl2. Flow cytometry and phase contrast microscopy showed morphological changes occurred in cultures exposed to 0.25 and 0.5 mg/L ZnCl2. Quantitative PCR (qPCR) analysis of selected cDNA samples showed significant upregulation of Mmt through all time points, significant upregulation of mcyC at a later time point. ELISA MC-LR analysis showed extracellular MC-LR (µg/L) and intracellular MC-LR (µg/cell) quota measurements persisted through 15 days, although 0.25 mg/L ZnCl2 treatment produced half the normal cell biomass and 0.5 mg/L treatment largely inhibited growth. The 0.25 and 0.5 mg/L ZnCl2 treated cells demonstrated a ~40% and 33% increase of extracellular MC-LR(µg/L) equivalents, respectively, as early as Day 5 compared to control cells. The 0.5 mg/L ZnCl2 treated cells showed higher total MC-LR (µg/cell) quota yield by Day 8 than both 0 mg/L ZnCl2 control cells and 0.1 mg/L ZnCl2 treated cells, indicating release of MCs upon cell lysis. This study showed this Microcystis aeruginosa strain is able to survive in 0.25 mg/L ZnCl2 concentration. Certain morphological zinc stress responses and the upregulation of mt and mcy genes, as well as periodical increased extracellular MC-LR concentration with ZnCl2 treatment were observed.

Optimization of FDA–PI method using flow cytometry to measure metabolic activity of the cyanobacteria, Microcystis aeruginosa

2011 ◽  
Vol 36 (9-11) ◽  
pp. 424-429 ◽  
Author(s):  
Xi Xiao ◽  
Zhi-ying Han ◽  
Ying-xu Chen ◽  
Xin-qiang Liang ◽  
Hua Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microcystis Aeruginosa ◽  
Metabolic Activity ◽  
Pi Method

Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry

2009 ◽  
Vol 94 (4) ◽  
pp. 255-264 ◽  
Author(s):  
Souad Hadjoudja ◽  
Chantal Vignoles ◽  
Véronique Deluchat ◽  
Jean-François Lenain ◽  
Anne-Hélène Le Jeune ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microcystis Aeruginosa ◽  
Chlorella Vulgaris ◽  
Copper Toxicity ◽  
Short Term

Ecotoxicological analysis of fly ash and rice-straw black carbon on Microcystis aeruginosa using flow cytometry

2013 ◽  
Vol 92 ◽  
pp. 51-56
Author(s):  
Liping Lou ◽  
Qiankun Yue ◽  
Feixiang Liu ◽  
Fang Chen ◽  
Baolan Hu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fly Ash ◽  
Black Carbon ◽  
Rice Straw ◽  
Microcystis Aeruginosa
Sign in / Sign up

Export Citation Format

Share Document