scholarly journals Demonstration of Deoxyribonucleic Acid in Colorectal Cancer Using Feulgen reaction

2020 ◽  
Vol 28 (2) ◽  
pp. 88-92
Author(s):  
Magdi M. Salih ◽  
Basem Elesawy ◽  
Salman M. Al-Ayli ◽  
Awad A. Al-Otaibi ◽  
Fares M. Al-Thobiti ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Deoxyribonucleic Acid ◽  
Feulgen Reaction

scholarly journals INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS

1963 ◽  
Vol 19 (3) ◽  
pp. 613-629 ◽  
Author(s):  
Sylvan Nass ◽  
Margit M. K. Nass
Keyword(s):  
Osmium Tetroxide ◽  
Deoxyribonucleic Acid ◽  
Proteolytic Enzymes ◽  
Blue Staining ◽  
Zinc Ions ◽  
Feulgen Reaction ◽  
Toluidine Blue Staining ◽  
Complete Digestion ◽  
Buffer Control ◽  
Dna Characteristics

The effects of proteolytic enzymes, ribonuclease, and deoxyribonuclease upon a fibrous component of chick embryo mitochondria, which was previously shown to have many fixation and staining properties characteristic of the bacterial nucleoplasm, are reported. Pepsin digestion of formaldehyde-fixed tissues removed the membranes and matrices of mitochondria, but a pepsin-resistant fibrous material remained which was heavily stained by uranyl and lead ions. Experiments on a DNA "model system" showed that DNA treated with osmium tetroxide can be depolymerized by deoxyribonuclease. Zinc ions strongly inhibited the depolymerization of DNA. Digestion of osmium tetroxide-fixed tissues (fixed only briefly) with deoxyribonuclease for 1 hour greatly reduced the Feulgen staining of the nuclei, and after 4 hours the Feulgen reaction was completely abolished. The reduction and the disappearance of the Feulgen reaction in nuclei was paralleled by partial to complete digestion of the mitochondrial fibers in the regions studied (after 1 and 4 hours, respectively), without any other obvious changes in cellular structures. When deoxyribonuclease was inhibited by the addition of zinc ions, the nuclear Feulgen reaction was not diminished, nor were the mitochondrial fibers removed. Buffer control incubations for deoxyribonuclease and ribonuclease did not alter the structure or staining properties of the mitochondrial fibers, nor did incubation with ribonuclease. The latter reaction digested the cytoplasmic and nucleolar ribosomes after a 4-hour incubation period, in parallel with the abolishment of toluidine blue staining. The results contribute further evidence that these mitochondria contain deoxyribonucleic acid.

The concentration of deoxyribonucleic acid in plasma from 73 patients with colorectal cancer and apparent clinical correlations

2008 ◽  
Vol 32 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Héctor Guadalajara ◽  
Carolina Domínguez-Berzosa ◽  
Mariano García-Arranz ◽  
María Dolores Herreros ◽  
Isabel Pascual ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Deoxyribonucleic Acid ◽  
Clinical Correlations

scholarly journals Peculiarities of cytochemical properties of cancer cells as revealed by study of deoxyribonucleoprotein susceptibility to Feulgen hydrolysis.

1977 ◽  
Vol 25 (7) ◽  
pp. 580-584 ◽  
Author(s):  
A V Zelenin ◽  
A A Kushch ◽  
T A Chebanu
Keyword(s):  
Acid Hydrolysis ◽  
Cancer Cells ◽  
Deoxyribonucleic Acid ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Feulgen Reaction ◽  
Squamous Carcinoma Cells ◽  
Schiff Reagent ◽  
Increased Sensitivity ◽  
Absorption Technique

Chromatin of human squamous carcinoma cells reacts more intensively to short (1-2 min) acid hydrolysis in the Feulgen reaction and is, after such treatment, more intensively stained by Schiff reagent than chromatin of normal cells of the same origin. To reveal this difference in chromatin properties the use of a fluorescence variant of the Feulgen reaction is necessary because nuclei-binding of Schiff reagent after such short hydrolysis is so weak that the amount of the stain bound by means of absorption technique is hardly possible. The use of increased sensitivity of cancer cells chromatin to acid hydrolysis for cancer cytodiagnosis is suggested, especially for the diagnosis of so called diploid cancers for which detection on the basis of deoxyribonucleic acid content determination is impossible.

scholarly journals DISCREPANCIES BETWEEN CYTOPHOTOMETRIC FEULGEN VALUES AND DEOXYRIBONUCLEIC ACID CONTENT

1971 ◽  
Vol 19 (3) ◽  
pp. 169-174 ◽  
Author(s):  
K. NOESKE
Keyword(s):  
Functional State ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Cell Types ◽  
Nuclear Proteins ◽  
Feulgen Reaction ◽  
Template Activity ◽  
Colored Product ◽  
Different Cell Types ◽  
Low Template

Diploid nuclei of different cell types of the granulocytopoietic and erythropoietic series showed different Feulgen values. The highest values were found in the most immature nuclei, and the lowest values in the most mature cells. Extraction of acid-soluble nuclear proteins brought the different values to the same level. Examples of similar findings in other cell types, as described in the literature, are discussed. The Feulgen reaction and its colored product depend on the functional state of the chromatin in such a way that nuclei with high template activity of deoxyribonucleic acid show high Feulgen values whereas mature, dense nuclei with low template activity have lower Feulgen values, although both kinds of nuclei contain identical amounts of deoxyribonucleic acid.

Comparison of deoxyribonucleic acid ploidy and nuclear expressed p62 c-myc oncogene in the prognosis of colorectal cancer

1990 ◽  
Vol 14 (4) ◽  
pp. 545-550 ◽  
Author(s):  
S. Rowley ◽  
K. M. Newbold ◽  
J. Gearty ◽  
M. R. B. Keighley ◽  
I. A. Donovan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Deoxyribonucleic Acid ◽  
Myc Oncogene

Méthode d'étude des quantités d'ADN métaphasique après écrasement de méristèmes radiculaires

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 706-708
Author(s):  
C. Le Coq ◽  
C. Guervin ◽  
M. Esclapez ◽  
J. Moret
Keyword(s):  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Feulgen Reaction ◽  
Root Cells ◽  
Meristematic Root

A method is described for standardized preparation of meristematic root cells treated with colchicine for cytophotométrie analysis of metaphase DNA. Deoxyribonucleic acid has been stained by the Feulgen reaction prior to crushing of the cells.Key words: cytophotometry, Ornithogalum, nuclear DNA content.

scholarly journals STUDIES ON THE FEULGEN REACTION WITH HISTOCHEMICAL MODEL SYSTEMS

1964 ◽  
Vol 12 (10) ◽  
pp. 758-767 ◽  
Author(s):  
M. J. HARDONK ◽  
P. VAN DUIJN
Keyword(s):  
Crystal Violet ◽  
Deoxyribonucleic Acid ◽  
Spectral Shift ◽  
The Other ◽  
Model Systems ◽  
Cellulose Film ◽  
Feulgen Reaction ◽  
Cell Nuclei ◽  
Amino Groups ◽  
Schiff Reaction

The absorption spectra of Feulgen-stained deoxyribonucleic acid (DNA)-containing membranes have been studied under various conditions. The absorption curve of the Feulgen-stained DNA-cellulose differs from those of the other model systems and is comparable to that of a solution of apurinic acid stained by the Schiff reaction. The spectral shift caused by the reaction of formaldehyde and SO2 with the stained films was used as a criterion for the degree of substitution of the bound pararosaniline. The spectrum of DNA-cellulose, unlike the spectra of the other models, did not show any change under the action of these reagents. This indicates that the three amino groups of pararosaniline are all bound to the DNA in a stained DNA-cellulose film. When the mobility of the apurinic acid chain is restricted, the formation of the trisubstituted product is apparently hindered. Such a situation may be present in DNA-containing polyacrylamide films containing proteins and also in cell nuclei. From a study of the literature it is concluded that the shoulder in the pararosaniline spectrum is most probably caused by a configurational isomer of the dye as indicated by Lewis et al. (16) for crystal violet. To explain the abnormal spectrum of the stained DNA-cellulose, it is proposed that in this case pararosaniline is bound with the three amino groups to three neighbouring deoxyribose groups in the hydrolyzed DNA. Studies with Stuart models of the molecules involved showed that in this case one conformation isomer of pararosaniline can be constructed more easily than the other. This situation may explain the spectrum of the stained DNA-cellulose. The other spectral results could also be interpreted on basis of this hypothesis.

scholarly journals DEOXYRIBONUCLEIC ACID CYTOPHOTOMETRY OF STAINED HUMAN LEUKOCYTES I. DIFFERENCES AMONG CELL TYPES

1969 ◽  
Vol 17 (4) ◽  
pp. 249-257 ◽  
Author(s):  
BRIAN H. MAYALL
Keyword(s):  
Visible Light ◽  
Dna Content ◽  
Close Agreement ◽  
Deoxyribonucleic Acid ◽  
Cell Types ◽  
Neutrophilic Granulocytes ◽  
Feulgen Reaction ◽  
Human Leukocytes ◽  
Intensity Measurements ◽  
Individual Human

The deoxyribonucleic acid (DNA) content of individual human leukocytes was estimated cytophotometrically using visible light and spreads stained either with gallocyanin-chrome alum following ribonuclease digestion or with the Feulgen reaction. When the cells were measured on a scanning cytophotometer, significant differences in stain intensity were found among slides. Significant differences also were found among the leukocyte types. In gallocyanin-chrome alum preparations, monocytes measured 16% higher than small lymphocytes and 13% higher than neutrophilic granulocytes. In Feulgen preparations, monocytes measured 4% higher than small lymphocytes and 6% higher than neutrophils. These differences among cell types were independent of donor and stain intensity. Measurements of cells within types and within slides frequently showed close agreement, but it is only in this very limited context that the data are consistent with the hypothesis of DNA constancy. Measurements made on a two-wavelength cytophotometer showed a divergence of only 2.1% relative to similar measurements made on the scanning cytophotometer, which suggests that the differences observed among cells and types are unlikely to be artifacts of the instruments. Over-all, the data indicate either that there is variability in DNA content or that DNA is not being expressed correctly by the measured stain content.

scholarly journals Cytological Evidence that Both RNA and DNA May Form a Complex with the Same Protein

1959 ◽  
Vol 5 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Virginia C. Littau
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Deoxyribonucleic Acid ◽  
Ovarian Follicle ◽  
Follicle Cells ◽  
Feulgen Reaction ◽  
Cytological Evidence ◽  
Trichloracetic Acid ◽  
Nucleoprotein Complex ◽  
Rna And Dna

Deoxyribonucleic acid can be added back to protein sites from which the original nucleic acid, ribo- or deoxyribo-, is removed. If sections of frozen-substituted ovarian follicle cells of a leafhopper are first extracted by hot trichloracetic acid to remove nucleic acids and then immersed in a solution of a commerical preparation of deoxyribonucleic acid, the nucleic acid becomes attached to nuclear and cytoplasmic sites and can be rendered visible by the Feulgen reaction. The addition occurs in certain other tissues as well. The results are discussed in relation to biochemical and other cytochemical investigations of the nucleoprotein complex.

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