Méthode d'étude des quantités d'ADN métaphasique après écrasement de méristèmes radiculaires

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 706-708
Author(s):  
C. Le Coq ◽  
C. Guervin ◽  
M. Esclapez ◽  
J. Moret
Keyword(s):  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Feulgen Reaction ◽  
Root Cells ◽  
Meristematic Root

A method is described for standardized preparation of meristematic root cells treated with colchicine for cytophotométrie analysis of metaphase DNA. Deoxyribonucleic acid has been stained by the Feulgen reaction prior to crushing of the cells.Key words: cytophotometry, Ornithogalum, nuclear DNA content.

scholarly journals THE SEX CHROMATIN AND HETEROCHROMATIC BODIES IN HUMAN DIPLOID AND POLYPLOID NUCLEI

1960 ◽  
Vol 8 (2) ◽  
pp. 345-364 ◽  
Author(s):  
Harold P. Klinger ◽  
Hans G. Schwarzacher
Keyword(s):  
X Chromosome ◽  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Liver Parenchyma ◽  
Interphase Nuclei ◽  
X Chromosomes ◽  
Human Amnion ◽  
Sex Chromatin

The deoxyribonucleic acid (DNA) content of Feulgen-stained interphase nuclei from human amnion epithelium and liver parenchyma as well as the DNA content of their sex chromatin or heterochromatic bodies was determined histophotometrically. In female nuclei the ratio between the nuclear DNA content and that of their sex chromatin or heterochromatic bodies remains constant irrespective of the polyploid state of the nucleus. Thus, in polyploid nuclei the heterochromatic bodies double their DNA content with each duplication of the diploid nuclear DNA content. Therefore, it is assumed that the large or multiple heterochromatic bodies of female polyploid nuclei are conjugated or multiple sex chromatin bodies. Some male diploid nuclei show a distinct heterochromatic body whose DNA content is about one-half that of a sex chromatin. About one-half of the polyploid male nuclei have heterochromatic bodies whose DNA content is approximately one-half of those of female nuclei of the corresponding polyploid class. This would indicate that in male diploid nuclei the single X chromosome sometimes leaves a heterochromatic rest one-half the size of a sex chromatin and in polyploid male nuclei the two or more X's may leave larger heterochromatic bodies. However, many male nuclei, even when polyploid, do not have distinct heterochromatic bodies. Possibly the heterochromatic portions of the X's have failed to join and form a sex chromatin-like body; or the Y inhibits the X from leaving a heterochromatic rest. The proposal of other authors that the sex chromatin is derived from but one of the X chromosomes of the female nucleus, the X in the male leaving no heterochromatic rest, is also considered. The DNA content does not vary significantly between nuclei with and without sex chromatin or heterochromatic bodies. It remains clear that female nuclei with abnormally large or multiple sex chromatin bodies and male nuclei with large distinct heterochromatic bodies are always polyploid.

Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum) cv. Coker 139

1983 ◽  
Vol 25 (2) ◽  
pp. 169-173 ◽  
Author(s):  
Sukhraj S. Dhillon ◽  
Earl A. Wernsman ◽  
Jerome P. Miksche
Keyword(s):  
Nicotiana Tabacum ◽  
Anther Culture ◽  
Dna Sequences ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chromosome Doubling ◽  
Nuclear Dna Content ◽  
Contributing Factors ◽  
Root Cells ◽  
Parental Cultivar

Nuclear DNA contents of a conventionally inbred flue-cured tobacco (Nicotiana tabacum L.) cultivar. 'Coker 139,' and two selected, doubled haploid (dihaploid) lines developed from it by anther culture and colchicine chromosome doubling were compared. The dihaploid lines had previously been shown to be lower yielding and agronomically inferior to the parental cultivar. Nuclei of leaf and root cells of the dihaploids possessed significantly higher amounts of DNA than the parental cultivar, although chromosome numbers (2n = 48) had not changed. Mean nuclear DNA content of the dihaploid lines was 10.62 pg, while nuclei of the parental cultivar contained 9.32 pg. The anther-culture process and (or) the nature of vegetative nuclei of pollen grains from which haploid plants originate have been suggested as possible contributing factors to higher DNA values. An average 12% increase in heterochromatin (condensed chromatin) in dihaploids, and significant differences in thermal denaturation of parental and dihaploid DNAs, suggest an amplification of DNA sequences during dihaploid formation.

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content
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