Production, biochemical characterization of a protease from Aspergillus oryzae and its application to protein hydrolysis for obtaining hydrolysates wits antioxidant activity

2012 ◽  
Author(s):  
Ruann Janser Soares de Castro
Keyword(s):  
Antioxidant Activity ◽  
Aspergillus Oryzae ◽  
Biochemical Characterization ◽  
Protein Hydrolysis

scholarly journals Biochemical Characterization of Cassiopea andromeda (Forsskål, 1775), Another Red Sea Jellyfish in the Western Mediterranean Sea

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 498
Author(s):  
Gianluca De De Rinaldis ◽  
Antonella Leone ◽  
Stefania De De Domenico ◽  
Mar Bosch-Belmar ◽  
Rasa Slizyte ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Characterization ◽  
Biological Activities ◽  
Enzymatic Digestion ◽  
Western Mediterranean ◽  
Western Mediterranean Sea ◽  
Blue Growth ◽  
Jellyfish Species ◽  
Italian Coasts

Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to reach Malta, Tunisia, and recently also the Italian coasts. Here we report on the biochemical characterization and antioxidant activity of C. andromeda specimens with a discussion on their relative biological activities. The biochemical characterization of the aqueous (PBS) and hydroalcoholic (80% ethanol) soluble components of C. andromeda were performed for whole jellyfish, as well as separately for umbrella and oral arms. The insoluble components were hydrolyzed by sequential enzymatic digestion with pepsin and collagenase. The composition and antioxidant activity of the insoluble and enzymatically digestible fractions were not affected by the pre-extraction types, resulting into collagen- and non-collagen-derived peptides with antioxidant activity. Both soluble compounds and hydrolyzed fractions were characterized for the content of proteins, phenolic compounds, and lipids. The presence of compounds coming from the endosymbiont zooxanthellae was also detected. The notable yield and the considerable antioxidant activity detected make this species worthy of further study for its potential biotechnological sustainable exploitation.

Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae

2011 ◽  
Vol 93 (3) ◽  
pp. 1157-1166 ◽  
Author(s):  
Ines Pisanelli ◽  
Petra Wührer ◽  
Yazmid Reyes-Dominguez ◽  
Oliver Spadiut ◽  
Dietmar Haltrich ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Aspergillus Nidulans ◽  
Aspergillus Oryzae ◽  
Biochemical Characterization

scholarly journals Biochemical Characterization of Traditional Varieties of Apricots (Prunus armeniaca L.) of the Campania Region, Southern Italy

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 100
Author(s):  
Florinda Fratianni ◽  
Antonio d’Acierno ◽  
Donatella Albanese ◽  
Marisa Di Matteo ◽  
Raffaele Coppola ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biochemical Characterization ◽  
Principal Component ◽  
Prunus Armeniaca ◽  
Campania Region ◽  
Important Region ◽  
Phenolic Profiles ◽  
Β Carotene

Campania is the most important region of Italy in the apricot cultivation, present mostly in the Vesuvio area. At least to the best of our knowledge, no studies are reporting the biochemical characterization of the considerable number of traditional apricot varieties present on this territory, including the qualitative and quantitative profile of the polyphenols present. Our work evaluated the content of β-carotene, total phenolics, phenolic profiles, ascorbic acid and antioxidant activity of 23 traditional varieties of apricots of the Campania region. Principal component analysis (PCA) highlighted that, in the two main groups, the antioxidant activity was strongly affected by the content of ascorbic acid (−0.89), or slightly affected by the content of total polyphenols (−0.67), respect to the content of ascorbic acid (−0.55), never by β-carotene. Chlorogenic acid (up to 55.07 μg g−1) and catechin (up to 96.15 μg g−1) resulted the most abundant polyphenols recognized through the chromatographic analysis. PCA, extended to the polyphenol profile, confirmed the distribution of the varieties in two large groups, evidencing once again the hierarchical distance of four varieties (“Panzona”, “Paolona” “Baracca” and “Boccucia Eboli”) compared to the others.

Biochemical characterization of extracellular fructosyltransferase from Aspergillus oryzae IPT-301 immobilized on silica gel for the production of fructooligosaccharides

2020 ◽  
Author(s):  
Larissa Lemos Faria ◽  
Sergio Andres Villalba Morales ◽  
José Pedro Zanetti Prado ◽  
Giancarlo de Souza Dias ◽  
Alex Fernando de Almeida ◽  
...  
Keyword(s):  
Silica Gel ◽  
Aspergillus Oryzae ◽  
Biochemical Characterization

scholarly journals Preponderance of Bioactive Medicinal Compounds and ATR-FTIR Spectroscopy of Coriander and Mustard Floral Honey from Apis mellifera

2017 ◽  
Vol 17 (3) ◽  
pp. 376
Author(s):  
Ishan Ullah Khan ◽  
Widhi Dubey ◽  
Vedprakash Gupta
Keyword(s):  
Antioxidant Activity ◽  
Ir Spectra ◽  
Biochemical Characterization ◽  
Total Phenolics ◽  
Chemical Constituents ◽  
Chemical Characteristic ◽  
Medicinal Compounds ◽  
Ic50 Value ◽  
Ft Ir

The physicochemical, total phenolics, flavonoids, and antioxidant activity were evaluated for biochemical characterization of coriander and mustard floral honey. The total phenolics, flavonoids and antioxidant activity were analyzed using UV-VIS spectrophotometer. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical characteristic of coriander and mustard floral honey. The total phenolics content was ranged from 294 to 462 mg gallic acid equivalent kg-1 of honey. The total flavonoid content was ranged from 43 to 53 mg quercetin equivalent kg-1 of honey. Antioxidant activity results were expressed as inhibitory concentration (IC50) value ranged from 4.58 to 5.54 mg mL-1. FT-IR spectra showed the presence of alcohols, carboxylic acids, esters, ethers, phenols, and amines in both floral honey samples. This study discovered that coriander floral honey is more affluent than mustard floral honey in nutritional as well as medicinal aspects. At a glance the processing of honey by heating did not affect the phenolics, flavonoid, and antioxidants of honey; even processed honey contains higher phenols and antioxidants. The FT-IR spectra showed the similarity in both kinds of honey refers to chemical constituents. This study will help the researcher and honey consumer to find out the higher bioactive medicinal compounds containing honey.

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