scholarly journals PROXIMATE AND PHYTOCHEMICAL ANALYSIS OF HEALTHY AND INFECTED MAIZE GRAINS IN ANAMBRA STATE

2020 ◽  
Vol 5 (2) ◽  
pp. 15-40
Author(s):  
C. E Anyaegbu ◽  
O.J Oledibe, ◽  
J.E Amadi

Four samples of stored maize grains were bought from Awka, Awkuzu, Onitsha and Nnewi. Direct and indirect methods of Fawole and Oso, 1998 were used to isolate the fungi responsible for the spoilage of post harvest maize grains after which four genera were isolated namely: Curvularia spp, Fusarium spp, Rhizopus spp and Aspergillus spp. They were identified using Barmette and Haunter, 1987 method of identification. The percentage of occurrence of the fungi from different samples shows that Aspergillus niger occured with a frequency of 31.5%, A. flavus with 31%, Rhizopus spp with 15.25%, Fusarium spp with 14.5% and the least Curvularia spp with 7.5%. AOAC method of 1999 was used to obtain the nutritional values of the maize grains. Good and spoilt maize grains were used in this research. Spoilt maize grains were used after four months of purchase. Anova were used to compare the nutritional values of both good and spoilt maize samples from the different locations. Six nutrients were obtained from both good and spoilt maize grains namely: crude protein, crude fibre, crude fat, moisture content, ash content and carbohydrate. The results differ in their values for both good and spoilt maize grains. The spoilt maize grains have little protein, fibre and fat and more of ash and carbohydrate. Further research was made on the phytochemical contents of maize and seven metabolites were obtained mamely: oxalate, phytate, tannin, phenol, alkaloid, flavonoid and glycoside. In order to reduce post-harvest grain loss of maize, periodical training of farmers on proper storage facilities are necessary

2011 ◽  
Vol 75 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Anna Kotlarz ◽  
Agnieszka Sujak ◽  
Wacław Strobel ◽  
Wilhelm Grzesiak

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and VarietyWe tested nutritional usability of pea seeds (Pisum sativumL.) at full maturity. Four white-flowering and 5 colour-flowering new Polish cultivars were compared. We determined proximate composition of ground seeds collected over 4 years period as well as amino acids, minerals (Ca, P, Na, K), tannins and fiber fractions (NDF, ADF, ADL). The seeds contained between 224 and 260 g·kg-1of crude protein which was rich in Lys (6.8±0.8 g) but poor in Met+Cys (2.0±0.2 g·16 g-1N). The amount of tannins (as tannic acid equivalent) in white-flowering cvs was 4.3±0.9 g and in colour-flowering - 7.4±2.2 g. The calculated protein nutritional values were compared against amino acid standards of human and animal nutrition and the whole egg protein. Effects of the cropping year and variety variance in respect of chemical composition of seeds were examined. Crude protein, crude oil, N-free extractives, tannins, K, amino acid content: Leu, Phe+Tyr and Ala content were influenced significantly by the cultivar, while the cropping year had a significant influence on dry matter, crude ash, crude protein, crude fibre, crude oil, N-free extractives ADF and ADL fiber fractions, content of P, Na and K, most of the amino acid levels and on nutritional values of the protein measured by means of CS and EAAI indices.


Author(s):  
Subhash Chandra ◽  
Sarla Saklani ◽  
Jen Tsung Chen

The aim of this research was to develop new antidibetic, anticancer, nephrolithiasis and new food supplements with potential pharmacological activity from readily accessed natural products. In the present study, we investigated the nutritional, mineral profile and phytochemical analysis of the extracts of C. indicus, D. biflorus, G. soja, B. cerifera, O. frumentaceu, E. coracana, P. frutenscens, S. italica, C. viscosa and H. vulgare seeds or pulps. The extraction process involved fractionation with various solvents whereas the estimation of nutrients and minerals was done using AOAC method and ICPMS techniques. The levels of crude protein, carbohydrates, crude fiber, crude fat and ash contents in C. indicus seeds were found to 2.20%, 14.12%, 56.38%, 7.50%, 2.20% and 5.30%, respectively whereas 5.93%, 72.07%, 5.10%, 1.00% and 3.90%, respectively were measured in the seeds of H. vulgare. This is the first scientific report to explore the comprehensive nutrients, mineral content and phytochemical analysis on the C. indicus, D. biflorus, G. soja, B. cerifera, O. frumentaceu, E. coracana, P. frutenscens, S. italica, C. viscosa and H. vulgare seeds and pulps. Which were found as highly medicinal and nutritional due to the presence of dietary fibre, protein, vitamins, minerals and other needful nutrients.


Author(s):  
Uyoyoghene O. Ududua ◽  
Michael O. Monanu ◽  
Lawrence C. Chuku

Aim: This study investigated the proximate and phytochemical composition of Brachystegia eurycoma leaves. Methods: Crude ethanol extract of B. eurycoma leaves was obtained by cold extraction method.  AOAC method was used for proximate analysis. Phytochemical profiling was done with qualitative phytochemical evaluation and gas chromatography-mass spectrometry (GC/MS) analytical method. Matching and interpretation of the spectral was done with the National Institute standard and Technology (NIST05) library. Results: The proximate analysis result showed B. eurycoma leaves to be abundant in param­eters evaluated in the order of 31.47±0.43% Carbohydrate > 15.15±0.04% Ash > 14.45±0.15 crude fibre > 13.83±0.32 protein > 13.14±0.22 moisture > 1.97±0.01 fat. Qualitative phytochemical analysis detected alkaloid, saponin, tannin, diterpenes, phenol, quinine, flavonoid, protein, xanthoprotein and cardiac glycoside in the leaves of B. eurycoma. GC/MS data showed that the prevailing volatile bioactive compounds in ethanol leaf extract of B. eurycoma were 3-O-Methyl-d-glucose (13.23%), cis-9-Hexadecenal (10.40%), Desulphosinigrin (10.34%), Phytol (7.58%), Hydroquinone (7.23%), n-Hexadecanoic acid (6.61%), Oleoyl chloride (6.10%), 9,12-Octadecadienoic acid (Z,Z)- (5.89%), Hexadecanoic acid, (2.97%), Benzofuran, 2,3-dihydro-(1.94%), Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) (1.92%). Conclusion: The result of this preliminary investigation reveals the potentials of B. eurycoma leaves as candidate for food, pharmaceutical, cosmetic and nutraceutical industries.


2018 ◽  
Vol 5 (10) ◽  
pp. 427-432
Author(s):  
Singaravelan Vivek ◽  
Segar Prakash

Yam is common name for the genus Dioscorea and considered as good sources of essential dietary nutrients. It has nutritional superiority over the tropical root crops. Understanding the nutritional values of yams, the nutrient contents of two yams, Dioscorea pentaphylla and Dioscorea oppositifolia have been analysed in the present study. During the study, the higher amount of moisture, crude protein, nitrogen, free sugars and buffer protein were estimated and analysed. The rich carbohydrate and calorific value, higher amount of fat and amino acids were observed in D. pentaphylla as compared to D. oppositifolia. The crude fiber and ash contents were comparatively higher in D. oppositifolia. Preliminary phytochemical analysis reveals the presence of alkaloids, flavonoids, glycosides, phenol, reducing sugars, saponins, steroids, tannins and triterpinoids in methanolic extract of both tubers.


2021 ◽  
Vol 46 (2) ◽  
Author(s):  
D. Uzama ◽  
S. C. Okolo ◽  
R. U. Okoh-Esene ◽  
A. B. Adebiyi ◽  
A.T. Orishadipe

Comparative Analysis on the Phytochemical, proximate and mineral composition of the seeds and peels of lime was carried out. The phytochemical, and proximate screenings were carried out using standard methods. The mineral analysis was carried out by using Atomic Absorption Spectrophotometer. The proximate analysis revealed that the seeds contain Moisture 8.20%, Ash 12.09%,Crude fat 8.50%,Crude Protein 1.93%,Crude fibre 20.71%, and Carbohydrate 48.58% while the peels contain Moisture10.60%, Ash 15.27%, Crude fat14.67%, Crude Protein 2.30%, Crude fibre23.36% and Carbohydrate 33.80%.The phytochemical analysis revealed the presence of alkaloid, saponins, flavonoids, terpenoids, phenols, and volatile oils in both the seeds and peels of the lime. In addition, the seeds contain steroids, while the peels contain tannins, balsams and anthraquinone. The mineral analysis revealed that the seeds contains Ni(0.17mg/100g),Pb(0.01mg/100g),Mn (0.20mg/100g),Zn(0.57mg/100g)Cr(0.17mg/100g),Mg(9.02mg/100g),Ca(27.02mg/100g),Cd(0.01mg/100g), Fe(2.55mg/100g) and Cu(4.81mg/100g) while the peels contain Ni(0.15mg/100g),Pd(0.21mg/100g),Mn(0,21mg/100g),Zn(0.23mg/100g),Cr(0.13mg/100g),Mg(7.61mg/100g),Ca(100.22mg/100g),Cd(0.01mg/100g),Fe(1.67mg/100g) and Cu(0.21mg/100g).The lime seeds and peels can serve as potential sources of drugs and nutrition with the seeds having higher concentration of minerals. 


2019 ◽  
Vol 15 (4) ◽  
pp. 363-366
Author(s):  
Subhash Chandra ◽  
Sarla Saklani ◽  
Ruchi B. Semwal ◽  
Deepak K. Semwal

Background: Barnyard millet is rich in protein, iron, calcium, phosphorus, fibre and vitamin content whereas finger millet is used in regular food and considered to be more nutritious than rice. Objective: The aim of this research was to develop new food supplements with potential pharmacological activity from readily accessed natural products. Methods: In the present study, we investigated the nutritional and mineral profile of the extracts of Eleusine coracana (L.) Gaertn. and Echinochloa frumentacea Link seeds. The extraction process involved fractionation with various solvents whereas the estimation of nutrients and minerals was done using AOAC method and ICPMS techniques. Results: The levels of crude protein, carbohydrates, crude fibre and ash content in E. coracana seeds were found to 7.30%, 68.10%, 3.60% and 8.50%, respectively whereas 2.87%, 80.93%, 5.80% and 5.30%, respectively were measured in the seeds of E. frumentacea. Conclusion: The seeds of E. coracana and E. frumentacea were found as highly medicinal and nutritional due to the presence of vitamins, minerals and other needful nutrients.


Author(s):  
S. O. Oyibo ◽  
G. C. Akani ◽  
C. C. Amuzie

This paper investigated the proximate, minerals and serum biochemistry in Hoplobatrachus occipitalis. The proximate and selected minerals of edible frog Hoplobatrachus occipitalis were determined using standard analytical methods. The result showed that crude protein was 16.91% carbohydrate was 1.76%, crude fibre 2.85%, The fat was 4.96% ash content was 1.84% and moisture was 71.67%. The selected mineral constituent recorded showed that sodium> iron> calcium > potassium > manganese. The nutritive serum biochemistry was determined in male and female species, the results revealed that both sexes have high nutritional profile suitable for human consumption; nevertheless, the female species have higher nutritional values than the males.


Author(s):  
Alejandro Grimm ◽  
Lill Eilertsen ◽  
Feng Chen ◽  
Renyuan Huang ◽  
Lars Atterhem ◽  
...  

Abstract This paper explores an alternative valorisation method for high-moisture content waste streams from the pulp and paper industry. Cellulose fibre rejects from industrial-scale recycling/pulping of waste paper was used as an ingredient in substrates for cultivation of Pleurotus ostreatus, commonly known as oyster mushroom. Three substrates with 40, 60, and 80 wt% fibre rejects were tested, and a conventional substrate formula based on birch (Betula ssp.) sawdust was used for comparison. The spent mushroom substrate (SMS) fuel characteristics were assessed through ashing procedures. Mycelium growth was faster on substrates based on fibre rejects. The average biological efficiencies of the first flush of fruit bodies were between 29 and 36% compared to 42% for birch sawdust substrates. The fruit bodies had good nutritional values, i.e., crude protein (22–25 wt% d.b.), crude fat (3–3.5 wt% d.b.), crude fibre (8–10 wt% d.b.), and carbohydrates (57–62 wt% d.b.). The concentrations of heavy metals, 5–10 µg/kg Pb, 19–28 µg/kg Cd, 5–6 µg/kg Hg, and 26–53 µg/kg As were well below the limit values for food products set in EU regulations. The SMS could be used as fuel for direct combustion or co-combusted with other biomasses. Graphic Abstract


Author(s):  
Hassan L. G. ◽  
◽  
Kabiru J Umar ◽  
Yuguda H

The present study was conducted to find out the nutritional values of Faidherbia albida (Acacia albida) seeds and pulp to be used as components in livestock feeds. The samples were randomly collected from various trees during the period of January to February. The samples were separated, milled and analysed for proximate components and mineral elements using standard methods of the AOAC. Proximate analysis results showed that the concentrations of tested parameters in the seed and the pulp respectively on % dry matter were: dry matter (90.0 + 0.4; 93.3 + 0.2), ash (3.3 + 0.1; 6.7 + 0.1), crude protein (20.6 + 1.2; 19.5 + 0.4), crude lipid (13.3 + 0.1; 3.3 + 0.1), crude fibre (6.7 + 0.1; 13.3 + 0.2), and available carbohydrate (46.1 + 0.1; 50.53 + 0.7). The results indicated that the contents of dry matter, ash, crude fibre and available carbohydrate were significantly higher (p < 0.05) in the pulp compared to the seed while the crude lipid values, however, were significantly higher (p < 0.05) in seeds compared to pulp. Crude protein values did not differ significantly (p > 0.05) between treatment means of seeds and pulp. The estimated energy value was significantly higher (p < 0.05) in the seed (1,682 kJ/100g) compared to pulp (1,363kJ/100g). The results for the mineral analysis indicate that the seeds and pulp contain appreciable concentrations (mg/100g) of Ca (136.6 + 5.0; 55.0 + 5.0); Mg (135.0 + 2.0 + 5.0; 84.7 + 3.0); P (26.6 + 0.9; 29.6 + 0.7); K (144.5 + 0.2; 88.8 + 0.1); Na (12.5 + 0.1; 6.8 + 0.1); Fe (6.2 + 0.2; 8.8 + 0.3); Cu (5.8 + 0.1; 2.5 + 0.1) and Zn (8.0 + 0.1; 3.0 + 0.1). Mineral elements in seed are significantly higher (p < 0.05) compared to that of pulp in most cases. From the results it can be inferred that the seed and pulp of F. albida could be an important source of feed ingredient for livestock if properly harnessed.


Author(s):  
Abdullahi Attah Alfa ◽  
Kokori Bajeh Tijani ◽  
O. D. Omotoso ◽  
Yahaya Junaidu ◽  
Abullahi Aminu Sezor

Cowpea (Vigna unguiculata) is an annual legume thought to have originated in Nigeria. Cowpea has high protein and carbohydrate content with a relatively low fat content and a complementary amino acid pattern to that of cereal grains make it an important nutritional staple food in the human diet. This study aims to determine the phytochemical, nutritional values and medicinal health aspects of brown, brown-black and white cowpea grown in Okene, Kogi State, Nigeria. Three colours of cowpea seeds are dried and ground in powder form. The crude powder was tested for alkaloids, anthraquinone, carbohydrates, cardiac glycosides, fat and oils, flavonoid, Saponins, steroidal ring, terpenoids and tannins. The proximate analysis of the samples was carried out for protein content, ash content, fat content, moisture content, crude fiber and carbohydrate after drying each sample at room temperature. Vitamins and mineral elements were determined by the dry ash extraction. The phytochemical components of secondary metabolites of the extracts from the obtained result, anthraquinone were absent in each of the three colour of the samples and tannins were not present in the white seed. The proximate composition of a nutritive value of each samples. The brown and brown-black seeds has higher value of percentages (%) crude protein, % ash content, % fat content, moisture content, % crude fibre, % carbohydrate of amounting to (57.84, 4.53, 11.60, 7.24, 3.40, 55.98) and (58.59, 4.45, 10.9, 7.06, 3.90, 54.60) while the white seed has (50.20, 3.52, 9.30, 8.56, 4.11, 59.50) has low % crude protein, % ash content and % fat content value compared to brown and brown-black seeds of cowpea. The vitamins components of brown, brown-black seeds and white seed of Cowpea where vitamin A, B, C, E (131, 232, 9, 7) in brown seed, (142, 221, 7, 9) in brown-black seed and (113, 209, 6, 5) in white seed. The Mineral element of brown, brown-black and white seeds of Cowpea where Na, K, Ca, Mg, Fe, Zn, P and Mn (49, 981, 651, 123, 54, 35, 620, 1.0) brown, (58, 789, 668, 98, 43, 38, 590, 2.0) brown-black and (63, 684, 723, 299, 10.11, 23, 843, 0.5) white seeds were detected but Cu and N non-detected in each. The brown and brown-black seeds of cowpea is richer in proximate, vitamins and mineral nutrients compared to white seed of cowpea. Therefore, adoption, utilization and consumption of cowpea grains as source of food will be a step forward towards combating the protein-calorie malnutrition and iron deficiency in this part of the world.


Sign in / Sign up

Export Citation Format

Share Document