scholarly journals Why did the Chicken cross the Ocean: an Analysis of Faunal Remains from the Emanuel Point Shipwrecks [University of West Florida, Pensacola]

Author(s):  
Zackariah David Pagels

The purpose of this research project is to determine, through analysis, what species of animals were being utilized onboard the Spanish ships during the Tristán de Luna expedition of 1559. In order to accomplish this goal, I will be analyzing faunal remains recovered from the three Emanuel Point Shipwrecks: EPI, EPII, and EPIII. This research is important because the analysis will allow us to better understand and interpret the lives of the Spaniards onboard the ships during the Luna expedition through their diet. In 1559, Luna attempted to create the first permanent settlement in Florida, the purpose of which was to construct a chain of missions along the gulf coast (Hudson 1989). These Missions would serve as a crucial component for both converting Native Americans and supporting previously shipwrecked Spaniards (Hudson 1989). The establishment of a permanent settlement in the gulf also had political implications; a colony would mark the Spaniards claim to La Florida and serve to prevent other countries from establishing their own colonies (Arnade 1959, Hudson 1989). Under the command of Luna were a total of eleven ships, carrying close to fifteen hundred individuals and enough supplies to last a year (Worth 2009). Food, or the lack thereof, was one aspect which served detrimental to the success of the expeditions which came before Luna (Worth 2009). Because of this, the Luna expedition was planned to include enough food to last the Spaniards until crops could be sown and harvested (Worth 2009). Not long after the Spanish arrived, however, Pensacola was struck by a powerful hurricane, sinking seven of Luna’s ships (Arnade 1959, Milanich 1995, Worth 2009). Most, if not all, of the supplies were still onboard the ships when they went down during the hurricane because a permanent storehouse had not yet been constructed (Arnade 1959, Milanich 1995, Worth 2009). Since the time of their sinking, 459 years ago, three of the seven ships that were lost have been discovered. The first Emanuel Point shipwreck was discovered in 1992 by a group of archaeologists from Florida’s Division of Historical Resources. The University of West Florida’s archaeology program joined the project in 1996. In total, 8,848 individual organic fragments have been recovered from Emanuel Point I alone; these include the remains of fish, shark, reptile, bird and mammal bones. All of these materials are kept in the maritime conservation lab, where they are processed and then conserved. Afterwards, they are moved to the Collection Management Building on campus for storage and future study by both faculty and students. In order for the remains to be analyzed, they must first be preserved through a process called desalination. This process, conducted in the laboratory incorporates the use of tap water and eventually deionized water to remove most, if not all, salts from the bones. After the bones are desalinated, they go through a process called consolidation. Consolidation allows the bones to be safely exposed to the atmosphere without deteriorating, warping, or excessively cracking; this is accomplished by soaking the bones in a solution of Elmer’s glue and water, usually a 50/50 solution, after which they can be left to air dry. Once the bones are desalinated, consolidated and dried, they are stable and can then be analyzed. I will be analyzing the faunal material for specific taphonomy characteristics linking them to consumption by humans. These taphonomic characteristics can include cut marks, breaks, bone splitting, and the presence of teeth marks; all of these can directly correlate the remains with butchering or consumption. To conduct species specific identification, I will be working with the Division of Anthropology and Archaeology’s faunal specialist, Mrs. Cathy Parker, and her comparative type collections. Through this analysis, I will be able to determine the specific species of animals that were being utilized as food by the Spanish and were onboard the ships during their sinking. This study will allow a new glimpse into the life of the Spanish sailors during Tristán de Luna’s fateful 1559 expedition to establish the first permanent settlement along Florida’s Gulf Coast.       References: Arnade, Charles 1959    Tristan de Luna and Ochuse (Pensacola Bay) 1559. The Florida Historical Quarterly 37(3/4): 201-222.   Hudson, Charles et al. 1989    The Tristan De Luna Expedition, 1559-1561. Southeastern Archaeology 8(1): 31-45   Milanich, Jerald 1995    Florida Indians and the Invasion from Europe. University Press of Florida, Gainesville, Florida   Milanich, Jerald 1999    Laboring in the Fields of the Lord: Spanish Missions and Southeastern Indians. Smithsonian Institution Press, Washington, DC.   Shirak, Andrey et al. 2012    DNA Barcoding Analysis of Fish Bones from a Shipwreck found at Dor, Israel. The Israeli Journal of Aquaculture, 2012.   Worth, John E. 2009    Documenting Tristan De Lunas Fleet, and the Storm that Destroyed It. The Florida Anthropologist 62(3/4): 83-92

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 456f-457 ◽  
Author(s):  
Ali O. Sari ◽  
Mario R. Morales ◽  
James E. Simon

Echinacea is a medicinal plant native to North America. It was used extensively by native Americans in the treatment of their ailments. It is presently one of the most popular medicinal plants in the United States. Its popularity has created a large market demand for the roots and foliage of the plant. The gathering of echinacea from the wild is leading to the reduction of native populations and the destruction of its genetic diversity. Cultivation of medicinal echinaceas is hindered by a low seed germination. Dormancy breaking studies were done on freshly harvested seeds of Echinacea angustifolia. Seed lots were placed under light at a constant temperature of 25 °C and at alternate temperatures of 25/15 °C for 14/10 h, respectively. Germination was more rapid and uniform and percent germination higher at 25 °C than at 25/15 °C. Seed tap-water soaking, dry heating, and sharp heating alteration did not increase germination. The application of 1.0 mM ethephon (2-chloroethylphosphoric acid) increased seed germination to 94% at 25 °C and 86% at 25/15 °C. Untreated seeds gave 65% germination at 25 °C and 11% at 25/15 °C. The application of 2500 mg·L–1 and 3500 mg·L–1 of GA to dry seeds and 2500 mg·L–1 to seeds that have been soaked under tap water and then dried increased germination to 82%, 83%, and 83% at 25 °C and 64%, 78%, and 64% at 25/15 °C, respectively.


2021 ◽  
Vol 8 ◽  
Author(s):  
Kim de Mutsert ◽  
Kristy A. Lewis ◽  
Eric D. White ◽  
Joe Buszowski

Coastal erosion and wetland loss are affecting Louisiana to such an extent that the loss of land between 1932 and 2016 was close to 5,000 km2. To mitigate this decline, coastal protection and restoration projects are being planned and implemented by the State of Louisiana, United States. The Louisiana Coastal Master Plan (CMP) is an adaptive management approach that provides a suite of projects that are predicted to build or maintain land and protect coastal communities. Restoring the coast with this 50-year large-scale restoration and risk reduction plan has the potential to change the biomass and distribution of economically and ecologically important fisheries species in this region. However, not restoring the coast may have negative impacts on these species due to the loss of habitat. This research uses an ecosystem model to evaluate the effects of plan implementation versus a future without action (FWOA) on the biomass and distribution of fisheries species in the estuaries over 50 years of model simulations. By simulating effects using a spatially-explicit ecosystem model, not only can the changes in biomass in response to plan implementation be evaluated, but also the distribution of species in response to the planned restoration and risk reduction projects. Simulations are performed under two relative sea level rise (SLR) scenarios to understand the effects of climate change on project performance and subsequent fisheries species biomass and distribution. Simulation output of eight economically important fisheries species shows that the plan mostly results in increases in species biomass, but that the outcomes are species-specific and basin-specific. The SLR scenario highly affects the amount of wetland habitat maintained after 50 years (with higher levels of wetland loss under increased SLR) and, subsequently, the biomass of species depending on that habitat. Species distribution results can be used to identify expected changes for specific species on a regional basis. By making this type of information available to resource managers, precautionary measures of ecosystem management and adaptation can be implemented.


2011 ◽  
Vol 11 (4) ◽  
pp. 418-425 ◽  
Author(s):  
S. W. Lam ◽  
H. B. Zhang ◽  
L. Yu ◽  
C. H. Woo ◽  
K. N. Tiew ◽  
...  

In this study, a quantitative species-specific polymerase chain reaction (PCR) method to rapidly detect E. histolytica in water is developed. First, the specificity of E. histolytica PCR detection was verified by using species-specific primers of 16S-like rRNA genes to clearly differentiate it from the closely related amoebae species E. dispar and E. moshkovskii. The sensitivity of this method was subsequently determined using purified E. histolytica genomic DNA and culture cells as PCR reaction templates. Results indicated that conventional PCR visualized on 1% agarose gel was able to detect as low as 0.02 pg genomic DNA and 5 cells, while real-time PCR could detect 0.01 pg genomic DNA and 2 cells of E. histolytica. The protocols for E. histolytica PCR detection in real water samples were then optimized by spiking E. histolytica cells into tap water and reservoir raw water samples. A two-round centrifugation treatment to concentrate amoeba cells directly as a PCR template was the most effective way to detect E. histolytica in spiked tap water samples, while DNA extraction after concentrating amoeba cells was required for spiked reservoir raw water samples. The detection limit of 50 E. histolytica cells in 100 ml tap water was achieved in 2 h from sample collection to real-time PCR data readout. With these established protocols, 78 tap water samples, 11 reservoir raw water samples and 4 feed water samples from Singapore water supply systems were analyzed by both conventional PCR and real-time PCR methods. No E. histolytica cell was detected in tested samples.


1973 ◽  
Vol 95 (1) ◽  
pp. 17-22 ◽  
Author(s):  
C. H. Marks

Measurements were made of the effect of frequency of formation on the velocity of air bubbles rising in a chain through distilled water, lap water, and sugar water. In all cases, increasing the frequency increased the rise velocity for a given bubble size. Measurements made in distilled water showed that the increase of velocity with frequency dropped off with bubble size until it was negligible for the smaller bubbles. It was shown that the variation of bubble velocity with frequency and size can be fairly well correlated with the velocity of rise of solitary bubbles by means of a model based on turbulent wake theory. Tap-water measurements showed the same effect of impurities in the water on the bubble rise velocity as had been observed for solitary bubbles; however, the bubble radius at which the effect became apparent decreased with frequency. Measurements made in sugar water showed that the effect of fluid properties on the rise velocity decreased as frequency increased. At the highest frequencies, no difference could be seen between the distilled water and the sugar water rise velocity curves.


2013 ◽  
Vol 8 (6) ◽  
pp. 549-560 ◽  
Author(s):  
Agne Krilaviciute ◽  
Nomeda Kuisiene

AbstractTaxonomy of thermophilic, endospore-forming bacteria has evoked a great interest over the past few years. Although a number of taxonomic markers were previously evaluated, their sequences in Geobacillus were too conservative, and identification of more variable markers is needed. Repetitive DNA is one of the promising variable targets in the development of the taxon-specific genotyping and identification schemes in bacteria. The aim of our study was to evaluate the possibility of using repetitive DNA in the taxonomy of Geobacillus. In this paper, we report the analysis of perfect tandem repeats of geobacilli. We focused on the long repeats (with a motif length of ≥20 nucleotides). This choice was based on the assumption that these motifs can be used for the construction of oligonucleotides — primers and probes. Thirty-three Geobacillus genus-specific motifs were identified in our work, fifteen of them were species-specific and fifteen — species cluster -specific. Three of them were genus-, but not species- or species cluster-specific. Some of the motifs were used for the construction of the primer pairs. The primers were validated by PCR. Out of 12 designed primer pairs, 11 were genus-specific and 4 — species-specific. Species-specific primers were successfully constructed for the phylogenetically defined species Geobacillus thermodenitrificans and Geobacillus toebii.


2007 ◽  
Vol 5 (3) ◽  
pp. 375-383 ◽  
Author(s):  
Bram M. W. Diederen ◽  
Caroline M. A. de Jong ◽  
Ingrid Aarts ◽  
Marcel F. Peeters ◽  
Anneke van der Zee

Our aim was to investigate the occurrence and identity of Legionella spp. in Dutch tap water installations using culture, real-time PCR and sequence analysis. The PCR assays used were a 16S rRNA gene based PCR with both a Legionella species specific probe and a L. pneumophila specific probe and a L. pneumophila-specific PCR based on the sequence of the mip gene. A total of 357 water samples from 250 locations in The Netherlands was investigated. The detection rates of Legionella spp. were 2,2% (8 of 357) by culture, and 87,1% (311 of 357) by PCR. The majority of samples was found to contain Legionella species other than L. pneumophila. These comprised of Legionella Like Amoebal Pathogens (LLAPs), L. busanensis, L. worsliensis and others. Fourteen (3,9%) samples were positive for L. pneumophila by either culture, 16S rRNA based PCR and/or mip based PCR. It is apparent from this study that Legionella spp. DNA is ubiquitous in Dutch potable water samples. Our findings further suggest that LLAPs and viable but nonculturable (VBNC) Legionella represent a large proportion of the population in man-made environments.


1970 ◽  
Vol 132 (2) ◽  
pp. 211-250 ◽  
Author(s):  
Tai Te Wu ◽  
Elvin A. Kabat

In an attempt to account for antibody specificity and complementarity in terms of structure, human κ-, human λ-, and mouse κ-Bence Jones proteins and light chains are considered as a single population and the variable and constant regions are compared using the sequence data available. Statistical criteria are used in evaluating each position in the sequence as to whether it is essentially invariant or group-specific, subgroup-specific, species-specific, etc. Examination of the invariant residues of the variable and constant regions confirms the existence of a large number of invariant glycines, no invariant valine, lysine, and histidine, and only one invariant leucine and alanine in the variable region, as compared with the absence of invariant glycines and presence of three each of invariant alanine, leucine, and valine and two each of invariant lysine and histidine in the constant region. The unique role of glycine in the variable region is emphasized. Hydrophobicity of the invariant residues of the two regions is also evaluated. A parameter termed variability is defined and plotted against the position for the 107 residues of the variable region. Three stretches of unusually high variability are noted at residues 24–34, 50–56, and 89–97; variations in length have been found in the first and third of these. It is hypothesized that positions 24–34 and 89–97 contain the complementarity-determining residues of the light chain—those which make contact with the antigenic determinant. The heavy chain also has been reported to have a similar region of very high variability which would also participate in forming the antibody-combining site. It is postulated that the information for site complementarity is contained in some extrachromosomal DNA such as an episome and is incorporated by insertion into the DNA of the structural genes for the variable region of short linear sequences of nucleotides. The advantages and disadvantages of this hypothesis are discussed.


2000 ◽  
Vol 6 (S2) ◽  
pp. 884-885
Author(s):  
Stephen C. Landers

The parasitic dinoflagellate Haplozoon is found in the intestine of marine polychaetes. It is composed of a chain of cells that hang from the intestinal wall into the lumen, and releases daughter cells from the posterior end of the chain which leave the host and reinfect other polychaetes. Few studies exist in the recent literature regarding Haplozoon and it has not been reported from the Gulf Coast of the United States. This study reports the genus Haplozoon from the maldanid polychaete Axiothella mucosa in St. Andrew Bay, Florida, and examines the structure of the organism by light and electron microscopy.Axiothella mucosa was collected in St. Andrew Bay, Florida and maintained in seawater at the Troy State University campus. Haplozoon spp. was prepared for whole mounts by smearing minced setigers of the worms onto a slide with a drop of seawater.


2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Mpho Defney Maje ◽  
Christ Donald Kaptchouang Tchatchouang ◽  
Madira Coutlyne Manganyi ◽  
Justine Fri ◽  
Collins Njie Ateba

The aim of this study was to characterise Vibrio species of water samples collected from taps, boreholes, and dams in the North West province, South Africa, and assess biocontrol potentials of their bacteriophages. Fifty-seven putative Vibrio isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar and identified using biochemical tests and species-specific PCRs. Isolates were further characterised based on the presence of virulence factors, susceptibility to eleven antibiotics, and biofilm formation potentials. Twenty-two (38.60%) isolates were confirmed as Vibrio species, comprising V. harveyi (45.5%, n = 10), V. parahaemolyticus (22.7%, n = 5), V. cholerae (13.6%, n = 3), V. mimicus (9.1%, n = 2), and V. vulnificus (9.1%, n = 2). Three of the six virulent genes screened were positively amplified; four V. parahaemolyticus possessed the tdh (18.18%) and trh (18.18%) genes, while the zot gene was harboured by 3 V. cholerae (13.64%) and one V. mimicus (4.55%) isolate. Isolates revealed high levels of resistance to cephalothin (95.45%), ampicillin (77.27%), and streptomycin (40.91%), while lower resistances (4.55%–27.27%) were recorded for other antimicrobials. Sixteen (72.7%) isolates displayed multiple antibiotic-resistant properties. Cluster analysis of antibiotic resistance revealed a closer relationship between Vibrio isolates from different sampling sites. The Vibrio species displayed biofilm formation potentials at 37°C (63.6, n = 14), 35°C (50%, n = 11), and 25°C (36.4%, n = 8). Two phages isolated in this study (vB_VpM_SA3V and vB_VcM_SA3V) were classified as belonging to the family Myoviridae based on electron microscopy. These were able to lyse multidrug-resistant V. parahaemolyticus and V. cholerae strains. These findings not only indicate the presence of antibiotic-resistant virulent Vibrio species from dam, borehole, and tap water samples that could pose a health risk to humans who either come in contact with or consume water but also present these lytic phages as alternative agents that can be exploited for biological control of these pathogenic strains.


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