ASSESSING THE COMBINING ABILITY OF RICE GENOTYPES IN F1 GENERATION USING COMPLETE DIALLEL CROSS

Author(s):  
I. A. Odhano ◽  
H. B. Bozdar ◽  
M. A. Sial

A complete diallel analysis was carried out to determine combining ability of Shandar, NIA-Mehran, NIA-19/A, KS-282 and Shua-92 rice varieties in F1 during 2017. The objective of the study was to identify potential recombinants for future rice breeding program. The research was conducted at Nuclear Institute of Agriculture, Tandojam. Genotypic mean squares were significant (P ?0.01) for all of the traits i.e.; days to heading, days to maturity, plant height, number of tillers plant-1, panicle length, grains panicle-1 and grain yield plant-1 indicating genetic variation. Importance of both additive and non-additive gene action was found with preponderance of additive genetic effects in expression of traits studied. Showing negative GCA effects, NIA-Mehran, NIA-19/A and Shua-92 proved better parent varieties for developing early maturity. While, NIA-Mehran, KS-282 and Shua-92 for developing short stature genotypes. While, Shandar remained the best combiner for yield and yield associated traits with higher positive GCA effects. Cross combination of Shandar × KS-282 was the best with higher negative effects for days to heading and maturity and higher positive effects for grain yield plant-1. NIA-Mehran × Shua-92 gave the highest negative SCA effects indicating best combination for short stature. In case of number of tillers plant-1 and grains panicle-1, the cross NIA-19/A × KS-282 was the better combination with the highest positive SCA effects. Shandar × NIA-Mehran manifested the highest value for panicle length. Findings of reciprocal SCA  revealed that the reciprocal cross combination of Shua-92 × Shandar was fruitful illustrating negative effects for days to heading and maturity. While, KS-282 × NIA-Mehran was the best reciprocal combination for yield and yield associated traits. It is concluded that rice varieties Shandar and NIA-Mehran could be used to improve yield contributing traits in rice, while, NIA-Mehran and KS-282 may be used to improve early maturity and short stature in rice crop.

2020 ◽  
Vol 24 (6) ◽  
pp. 549-556
Author(s):  
O. P. Kibalnik ◽  
L. A. Elkonin

Investigation of the effect of the cytoplasm on the combining ability (CA) of lines with cytoplasmic male sterility (CMS) is of considerable interest in terms of understanding the genetic functions of the cytoplasm and for practical purposes to create hybrids with improved economically valuable traits. In order to investigate the effect of different types of sterile cytoplasm (A3, A4, 9E) on CA in sorghum, we studied the manifestation of a number of biological and agronomic traits in 54 F1 hybrid combinations obtained using iso-nuclear CMS lines with the nuclear genome of the line Zheltozernoye 10, differing only in the types of sterile cytoplasm (A3, A4 and 9E). Eighteen varieties and lines of grain sorghum developed at the Russian Research and Project-technological Institute of Sorghum and Maize were used as paternal parents. The CA was determined by the topcross method. F1 hybrids and their parents were grown in 2015–2017 in conditions of insufficient (2015–2016: HTC (hydro-thermal coefficient) = 0.32–0.66), or good water availability conditions (2017: HTC = 1.00). On average, for three years of testing, a positive effect of the 9E cytoplasm on the general combining ability (GCA) (0.63) and negative effects of the A3 and A4 cytoplasms (–0.32 and –0.31) for the inflorescence length were noted. In dry seasons, significant positive effects of the 9E cytoplasm on GCA for the length of the largest leaf, and positive effects of the A3 cytoplasm on GCA for the plant height, and negative effects of the A4 cytoplasm on GCA for these traits were observed. No differences were observed during the wet season. The type of CMS did not affect the GCA for the width of the largest leaf and grain yield. The dispersion of specific combining ability (SCA) in the dry seasons was significant for the following traits: leaf length, plant height, panicle length and width, and grain yield, the 9E cytoplasm had the highest SCA dispersion, whereas the A4 cytoplasm had the smallest one. The data obtained indicate that different types of sterile cytoplasm of sorghum make a different contribution to CA under conditions of drought stress.


Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Mehrzad Allahgholipour ◽  
Ezatollah Farshdfar ◽  
Babak Rabiei

This study was conducted to determine the combining ability and heritability of rice grain yield, its components and some grain quality traits such as amylose content (AC), gelatinization temperature (GT), gel consistency (GC) and head rice recovery (HRR). The study was commenced by crossing the selected rice varieties based on a full diallel mating design. The F1 was harvested at the end of the season. In the following season, the crossed, reciprocal and parental lines were planted in randomly complete block design with three replications. Analysis of variance indicated that genotypes were significantly different for all traits. The diallel analysis by Griffing`s method showed highly significant differences for GCA for number of panicles per plant (PN), amylose content, gelatinization temperature and head rice recovery. Highly significant differences were also observed for both SCA and REC for all evaluated characters. The results showed that the grain yield (GY), number of filled grains (FGN), 100-grain weight (HGW) and GC were controlled by non-additive gene action, while the inheritance of PN, AC, GT and HRR were largely controlled by additive gene effects, although non- additive genetic components and reciprocal effect were also involved, which suggest that a selection process could be done in the early generations. The two improved lines (RI18442-1 and RI18430-46) were found to be good general combiners for GY and FGN, while the best combiners for PN was Tarom Mohali and IR50 and for HGW was RI18430-46. The best combinations for GY were RI18430-46 ? IR50, Tarom Mohali ? RI18447-2 and Daylamani ? RI18430-46. The good hybrids were Tarom Mohali ? IR50, Line23 ? RI18447-2 and Line23 ? Backcross line for AC. Narrow sense heritability showed that the GY and GC had the lowest values while the other traits had either moderate or high heritability, which indicates selection in the early generations could be done to fix the favorable genes. In present study, narrow sense heritability was high for AC and moderate for GT, PN and HRR.


2015 ◽  
Vol 26 (2) ◽  
pp. 23-32
Author(s):  
M. A. Islam ◽  
M. A. K. Mian ◽  
M. G. Rasul ◽  
Q. A. Khaliq ◽  
M. K. Bashar

To study combining ability effects of developed CMS and restorer lines an experiment was carried out at the experimental farm, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur during Aman 2011 and Boro 2011-12 following RCBD design through line × tester analysis in three replications. Five known CMS lines and sixteen developed Restorer lines were considered for through line × tester analysis. Among sixteen restorer lines, only four showed significant negative GCA effects for all these three traits for earliness. The estimated of gca effects of parents indicated that seven parents and sixteen crosses contributed highly significant negative effects for plant height which were responsible for dwarfing character. Fifteen crosses and eight parents showed significant negative sca estimates for days to first flowering and twenty crosses for maturity. Considering both SCA effects and per se performances for days to 1st flowering and maturity; the crosses of IR 58025A with RG-BU08-005R, RG-BU08-006R, RG-BU08-016R, RG-BU08-018R and RG-BU08-025R were found as the best specific combiner. Among 80 crosses fifty two crosses showed significant positive SCA effects along with above average perse performances for grain yield. Ten crosses of IR 58025A, seven crosses of GAN46A, fourteen crosses of IR 62829A, nine crosses of IR 68888A and ten crosses of BRRI 1A showed significant positive SCA effects along with mean values resulting significant positive SCA effects and above average perse performances with all five CMS lines for grain yield.


2021 ◽  
Author(s):  
Bruce Mutari ◽  
Julia Sibiya ◽  
Edmore Gasura ◽  
Prince Muchapondwa Matova ◽  
Kennedy Simango ◽  
...  

Abstract Knowledge of the genetic basis of navy bean (Phaseolus vulgaris L.) performance under drought stress (DS) is important for planning appropriate breeding and selection strategies in DS environments. Twenty-eight F2 progenies generated from an 8 x 8 half-diallel mating design were evaluated to determine combining ability effects and mode of gene action of grain yield (GYD) and yield attributing traits in navy bean under DS and non-stressed (NS) conditions. The experiments were conducted in two locations in a 6 x 6 square lattice design with two replications during the 2020 dry season. There were significant (p < 0.001; p < 0.05) positive correlations for number of pods per plant (NPPP), number of seeds per plant (NSPP) and 100-seed weight (SW) with GYD under both DS and NS. General and specific combining ability (GCA; SCA) effects were significant (p < 0.05) under both DS and NS for most traits indicating the importance of both additive and non-additive gene effects in the expression of the traits. Parents with best combining ability for most of the studied traits were G1, G7, G6 and G8 under NS, and G3, G4, G7 and G8 under DS. The most promising progenies with high values for GYD and its component traits under DS were G2 X G3, G2 X G8, G4 X G5, G4 X G8, and G6 X G8. Good general and specific combiners with high significant positive effects under DS should be used further in breeding for moisture stress tolerance.


2021 ◽  
Vol 22 (3) ◽  
pp. 289-298
Author(s):  
Amitava Roy ◽  
Anil Kumar ◽  
Vaishali Rawat ◽  
Anu Singh

The present research investigation was conducted in order to analyze combining ability and nature of gene actions in 33 F1s of bread wheat (Triticum aestivum L. em. Thell) developed by crossing eleven lines with three testers in a line x tester mating design in terms of grain yield and associated traits. The hybrids along with the parental lines and two check varieties were planted in a randomized block design in three replications. Variance ratio between general and specific combining ability was found to be less than unity which indicated the prevalence of non-additive gene actions involved in the inheritance of these characters. Parental lines QLD 75 (3.164), followed by SOKOLL (2.888) and QLD 65 (2.819) exhibited significant GCA for grain yield, while another line PRL/2*PASTOR*2//FH6-1-7/3/KINGBIRD#1//… was observed to be the better general combiner for most other traits including maturity. Among the hybrids cross combination NAC/TH.AC//3*PVN/3/MIRLO/BUC/4/2*PASTOR/5/…x HD 3237 showed significant higher positive SCA for grain yield and biological yield per plant, whereas, F1 QLD 75 x HI 1621was a good specific combiner for harvest index (%) and number of grains per spike. The cross combination VORB/4/D67.2/PARANA66.270 x PBW 725 was observed with significant higher positive SCA for days to 75% heading, days to maturity and plant height, and another hybrid VORB/4/D67.2/PARANA 66.270 x HI 1621 exhibited significant positive SCA for 1000 grain weight and spike length.


2018 ◽  
Vol 3 (01) ◽  
pp. 34-43
Author(s):  
Vivek Kumar Singh ◽  
Bijendra Kumar ◽  
Lokendra Singh ◽  
Sourabh Panwar ◽  
Sandeep Kaur ◽  
...  

Estimation of combining ability by half diallel analysis and magnitude of heterosis was conducted at Section of Rabi Cereals, C. S. A. University of Agriculture Science and Technology, Kanpur, UP, in a set of 10 parents received from Economic Botanist Region, Section of Rabi Cereals, Kanpur by making 45 cross combinations evaluated during winter and Rabi 2012-13 in RBD with three replications. The data on nine characters viz. days to 50 % flowering, Plant height, No. of tillers/plant, Spike length/plant, No. of grains/main spike, Days to maturity, 1000-grain weight and Grain yield/plant were utilized for present study. On the basis of GCA effects across eight charactersK9423 and K9533 (-0.76) are good and desirable for days to 75% flowering for crop improvement, grain yield per plant observed for PBW502 and PBW343. The most promising crosses showing significantly positive SCA effects for yield per plant K9423xHD2285and PBW502xPBW343, days to maturity is showing significantly negative SCA effect for NW2036xHD2285 (-9.68). Heterosis is estimated highest over better parent for yield per plant was HD2285xK9533 (16.15). These crosses should be exploited for further advancement in breeding programme for HYVs and early maturity, simultaneously.


2019 ◽  
Vol 11 (3) ◽  
pp. 436-439
Author(s):  
Ghaffar KIANI

Rice is staple food in Iran. Despite of high quality of local rice, their grain yield is low. In hybridization breeding programs, selection of suitable parents is an essential role for developing new combinations with broadens genetic diversity. Combining ability of local rice varieties namely ‘Hashemi’, ‘Sang Jo’ and ‘Tarom Deylamani’ and ‘Nemat’ was evaluated in a partial diallele analysis for agronomic traits in a randomized complete block design at Sari Agricultural Sciences and Natural Resources University. General combining ability (GCA) and specific combining ability (SCA) variances showed predominated role of additive gene effects in the inheritance of grain length. Both additive and non-additive components of genetic variances were important in the inheritance of traits like grain yield, plant height, panicle length, total grains per panicle, grain length and grain length to width. However, non-additive gene effects were seen for tiller number. Results showed that ‘Nemat’ was the best general combiner for most of characters followed by ‘Tarom Deylamani’. The cross of ‘Hashemi’ × ‘Tarom Deylamani’ was suggested to exploitation of heterosis breeding for increasing yield and its components in rice breeding programs. 


2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Emmanuel Yaw Owusu ◽  
Haruna Mohammed ◽  
Kulai Amadu Manigben ◽  
Joseph Adjebeng-Danquah ◽  
Francis Kusi ◽  
...  

Information on combining ability and reciprocal effects (REC) facilitates efficient utilization of genetic materials in a breeding program. This study was conducted (at the CSIR-Savanna Agricultural Research Institute, Ghana) to determine general combining ability (GCA) and specific combining ability (SCA), heritability, genetic advance, GCA, and SCA effects as well as the relationship between parents per se performance and progenies for yield components and maturity traits in cowpea. The test populations were derived using a 5 × 5 complete diallel cross of parents with different yield attributes and maturity durations. The results indicated that GCA was predominant for number of days to 90% pod maturity, plant height at maturity, and hundred-seed weight. This showed that genes with additive effects conditioned these traits. Padi-Tuya, Songotra, and IT86D-610 were identified as good general combiners for grain yield, while Sanzi-Nya was identified as a general combiner for developing extra-early duration cowpea varieties. Crosses Songotra × Sanzi-Nya, SARC-1-57-2 × IT86D-610, Songotra × SARC-1-57-2, and Padi-Tuya × Songotra were identified as good specific combiners for days to 50% flowering, pod length, pods per plant, pod yield, grain yield, and seeds per pod. The findings from this study provide useful information on the inheritance of early maturity and yield traits in cowpea. This can be exploited to develop high yielding and early maturing cowpea varieties as climate smart strategy to mitigate climate change via breeding methods such as pedigree selection and marker assisted backcrossing (MABC). Pedigree selection method is being used to develop varieties from the hybrid with high and significant SCA for grain yield, whereas the development of extra-early duration varieties via MABC with Sanzi-Nya (general combiner for earliness traits) as a donor parent is ongoing.


Author(s):  
Sameena Begum ◽  
B. Srinivas ◽  
V. Ram Reddy ◽  
Ch. Aruna Kumari

The present investigation was carried out to understand the interrelationship and degree of dependence of grain yield on its components and gall midge incidence and to elucidate their relative importance. The experiment was conducted using 42 rice hybrids developed by crossing 6 CMS lines and seven testers in Line X Tester mating design, their parents and four checks. Observations were recorded on gall midge incidence, grain yield and fourteen component characters. Correlation coefficient analysis suggested that grain yield per plant had highly significant and positive association with panicle length (rp = 0.4600**, rg = 0.5545**) followed by number of grains per panicle (rp = 0.4219**, rg = 0.5125**) and number of productive tillers per plant (rp = 0.4006**, rg = 0.4942**) and negative non significant association with gall midge incidence (rp =-0.1154, rg =-0.1430). Panicle length had the highest positive direct effect (0.9688) followed by the number of grains per panicle (0.5748). Gall midge incidence showed a negative direct effect at the phenotypic level and a positive direct effect at the genotypic level on the grain yield per plant. Further, it showed indirect negative effects via plant height, number of productive tillers per plant, number of grains per panicle, spikelet fertility, hulling percentage, milling percentage, kernel length and kernel breadth as revealed from path analysis. The result of multiple linear regression exhibited that only the number of productive tillers per plant and the number of grains per panicle contributed significantly towards grain yield per plant. The step-wise regression analysis revealed that the panicle length, number of grains per panicle and number of productive tillers per plant were the most important characters having R=0.6505 and thus, could explain 42.31% of the total variation of grain yield. Gall midge incidence contributed to only 0.95% of the variation for grain yield, which might be due to the low incidence or resistance of the hybrids to the gall midge.


2020 ◽  
Vol 10 (1) ◽  
pp. 76-84
Author(s):  
B. V. Dziubetskyi ◽  
V. Yu. Cherchel ◽  
O. V. Abelmasov ◽  
V. V. Semenova ◽  
M. M. Tagantsova

The aim of the study: Evaluation of sister hybrids obtained on the basis of early maturity Iodent germplasm inbred lines (FAO 180-250) as a source material for the development of new inbred lines and evaluation of this material’s main breeding qualities as well as of level of hypothetical heterosis in sister crosses and their polymorphism as an integral part of the breeding cycle. Methods: Field method, mathematical-statistical method. Results: The studies investigated sister hybrids obtained by crossing of 8 precocious Iodent germplasm inbred lines, their combining ability for “grain yield” trait and determined whether they can be used as a source material for the synthesis of new homozygous inbred lines. The results of the evaluation of sister hybrids and their testcrosses on the basic economically valuable traits are presented. According to research results in 2013-2015, it was found that sister hybrids with the least variable grain yields by years of research were obtained from the crossing of DK1274 and DK237 inbred lines. The calculations of the correlation coefficients between hypothetical heterosis and genetic distances revealed no significant correlation between them (r=0.118-0.359) as well as between the yield of sister hybrids and genetic distances (r=0.167-0.351). It was found that the least variable indicators of grain yield for the years of research were noted in sister hybrids obtained on the basis of source inbred lines with the most stable manifestation of this indicator (r=0.714). Sister hybrids were noted, which had effects of general combining ability at class 2, regardless of study conditions (DK216 × DK213, DK237 × DK1274, DK714/195 × DK216), indicating the stability of genotype response to growing conditions. Conclusion: The evaluation of 8 precocious constant Iodent germplasm inbred lines on breeding traits revealed the best of them (DK555 and DK1274) in combining ability compared to the DK744 control inbred line. Significant influence of the conditions of research years on all the traits studied was noted. The study of sister hybrids has revealed forms with a significant manifestation of hypothetical heterosis. It was the highest on average in crosses of inbred lines DK234 (137.0%), DK714/195 (122.7%) and DK216 (101.1%), and the smallest in hybrids with inbred lines DK555 (58.5%), DK1274 (65.8%) and DK744 (75.0%). Sister combinations with DK1274 and DK237 inbred lines were characterized by the highest yield stability over the years of study. In determining genetic distances, the most genetically similar inbred lines DK744 (0.136) and DK234 (0.197) to inbred line 207 (P165), which is the classic representative of the Iodent germplasm, are noted.


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