scholarly journals FREE RADICAL SCAVENGING POTENCY OF FIVE DATE PALM (PHOENIX DACTYLIFERA L.) FRUIT VARIETIES CULTIVATED IN KHAIRPUR MIR’S

2021 ◽  
Vol 36 (2) ◽  
pp. 101-105
Author(s):  
Z. A. Mahar ◽  
G. Q. Shar ◽  
A. R. Shaikh
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Date Palm ◽  
Water Solution ◽  
Radical Scavenging ◽  
Phoenix Dactylifera ◽  
Free Radical Scavenging ◽  
Mature Stage ◽  
Phoenix Dactylifera L ◽  
Scavenging Efficiency

The fruit of date palm (Phoenix dactylifera L.) is well-known for its several health blessings and dietary values and its wonderful supply of phenolics compounds and holds relatively exquisite antioxidant capacity. Therefore, the goal of this study is to investigate the free radical scavenging efficiency of the five-date types Aseel, Kupro, Karbala Wari, Asul Khurmo and Gajar Wari cultivated in district Khairpur Mir’s. These date varieties were screened with the help of DPPH assay to evaluate their free radical scavenging efficiency in different solvents. From the statistics, it turned into scrutinize that each extract has interesting antioxidant functionality against free radicals. The methanolic extract has more potential to incline the free radicals.  It was concluded that the date variety Asul Khumro is shown excellent activity because it possesses lowest IC50 value 17.823µg/mL, followed by Gajar Wari 20.679 µg/mL in methanolic solution while Kupro 20.937µg/mL in Methanol + Water solution then relaxation of different date varieties at their mature Tamar level. It is concluded from the results that all dates fruit varieties at their final mature stage holds higher antioxidant potency and indicated that these date fruits have natural antioxidants.

Free radical scavenging efficiency of Nano-Se in vitro

2003 ◽  
Vol 35 (7) ◽  
pp. 805-813 ◽  
Author(s):  
Bo Huang ◽  
Jinsong Zhang ◽  
Jingwu Hou ◽  
Chang Chen
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Efficiency

scholarly journals Modulation of rabbit muscle sarcoplasmic reticulum Ca2+-ATPase activity by novel quercetin derivatives

2013 ◽  
Vol 6 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Dušan Blaškovič ◽  
Petronela Žižková ◽  
Filip Držík ◽  
Jana Viskupičová ◽  
Miroslav Veverka ◽  
...  
Keyword(s):  
Heart Failure ◽  
Free Radicals ◽  
Free Radical ◽  
Sarcoplasmic Reticulum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rabbit Muscle ◽  
Scavenging Activity ◽  
Quercetin Derivatives

Abstract Sarcoplasmic reticulum Ca2+-ATPase (SERCA) is the pump crucial for calcium homeostasis and its impairment results in pathologies such as myopathy, heart failure or diabetes. Modulation of SERCA activity may represent an approach to the therapy of diseases with SERCA impairment involvment. Quercetin is flavonoid known to modulate SERCA activity. We examined the effect of nine novel quercetin derivatives on the activity of the pump. We found that 5-morpholinohydroxypoxyquercetin, di(prenylferuoyl)quercetin, di(diacetylcaffeoyl)-mono-(monoacetylcaffeoyl)quercetin and monoacetylferuloylquercetin stimulated the activity of SERCA. On the contrary, monochloropivaloylquercetin, tri(chloropivaloyl)quercetin, pentaacetylquercetin, tri(trimethylgalloyl)quercetin and diquercetin inhibited the activity of the pump. To identify compounds with a potential to protect SERCA against free radicals, we assessed the free radical scavenging activity of quercetin derivatives. We also related lipophilicity, an index of the ability to incorporate into the membrane of sarcoplasmic reticulum, to the modulatury effect of quercetin derivatives on SERCA activity. In addition to its ability to stimulate SERCA, di(prenylferuloyl)quercetin showed excellent radical scavenging activity.

scholarly journals Assessment of Free Radical Scavenging Potential and Oxidative DNA Damage Preventive Activity ofTrachyspermum ammiL. (Carom) andFoeniculum vulgareMill. (Fennel) Seed Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nandini Goswami ◽  
Sreemoyee Chatterjee
Keyword(s):  
Dna Damage ◽  
Free Radicals ◽  
Free Radical ◽  
Oxidative Dna Damage ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Dpph Radical ◽  
Preventive Activity ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

Oxidation of biomolecules such as carbohydrates, proteins, lipids, and nucleic acids results in generation of free radicals in an organism which is the major cause of onset of various degenerative diseases. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. The present study was designed to examine the free radical scavenging potential and oxidative DNA damage preventive activity of traditionally used spicesTrachyspermum ammiL. (carom) andFoeniculum vulgareMill. (fennel). The aqueous, methanolic, and acetonic extracts ofT. ammiandF. vulgareseeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents. Free radical scavenging potential was investigated using standard methods, namely, DPPH radical scavenging assay and ferric reducing antioxidant power assay along with the protection against oxidative DNA damage. The results stated that acetonic seed extracts (AAcSE and FAcSE) of both the spices possessed comparatively high amount of total phenolics whereas methanolic seed extracts (AMSE and FMSE) were found to have highest amount of total flavonoids. At 1 mg/mL concentration, highest DPPH radical scavenging activity was shown by FMSE (96.2%), AAcSE was recorded with highest FRAP value (2270.27 ± 0.005 μmol/L), and all the seed extracts have been shown to mitigate the damage induced by Fenton reaction on calf thymus DNA. Therefore, the study suggests thatT. ammiandF. vulgareseed extracts could contribute as a highly significant bioresource of antioxidants to be used in our day-to-day life and in food and pharmaceutical industry.

scholarly journals Antioxidant Activity of Date Palm Fruit (Phoenix dactylifera L.) Extract for Oxidative Stabilisation of Butter Oil at Ambient Temperature

2015 ◽  
Vol 58 (2) ◽  
pp. 59-64
Author(s):  
Fazal Rahman ◽  
Muhammad Nadeem ◽  
Sabir Khan ◽  
Shakeel Ahmad ◽  
Yasir Zahoor
Keyword(s):  
Linoleic Acid ◽  
Ambient Temperature ◽  
Date Palm ◽  
Radical Scavenging ◽  
Phoenix Dactylifera ◽  
Oxidation Products ◽  
Total Phenolic ◽  
Palm Fruit ◽  
Butter Oil ◽  
Phoenix Dactylifera L

In this study, long term preservation of butter oil was achieved through ethanolic extract of date palm fruit (Phoenix dactylifera L.). Butter oil was supplemented with date palm fruit extract (DPFE) at three different concentrations i.e. 250, 500 and 750 ppm (T1, T2 and T3) and compared with a control. Total phenolic content, DPPH free radical scavenging activity and inhibition of linoleic acid peroxidation of the DPFE was 5.19 GAE, 74.2 and 81%, respectively. IC50 value of date extract for the inhibition of DPPH and linoleic acid peroxidation was 2.45 and 0.82 mg/mL, respectively. The loss of oleic acid and linoleic acid in control after six months of storage was 16 and 52% as compared to T3 which was 4% and 14%. T3 yielded the lowest concentration of primary and secondary oxidation products with no effect on sensory attributes. DPFE can be used to enhance the shelf life of  butter oil at ambient temperature. 

scholarly journals Phenolic content, antioxidant and anti-inflammatory activities of seeds and leaves of date palm (Phoenix dactylifera L.)

2019 ◽  
Vol 5 ◽  
Author(s):  
Jenny A. John ◽  
Fereidoon Shahidi
Keyword(s):  
Date Palm ◽  
Ldl Cholesterol ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Phoenix Dactylifera ◽  
Cholesterol Oxidation ◽  
Total Phenolic ◽  
In Vitro Tests ◽  
Phoenix Dactylifera L ◽  
Anti Inflammatory

The total phenolic content of date palm (Phoenix dactylifera L.) seeds (samples 1 and 2) and leaves were determined and for the first time their antioxidant activity in a food system investigated. The anti-inflammatory activity and potency of samples in the inhibition of radical-induced DNA scission and human low-density lipoprotein (LDL) cholesterol oxidation was also evaluated. The total soluble phenolic content of the date palm leaves was 106.96 mg gallic acid equivalents (GAE)/g sample and ranged from 68.73 to 82.62 mg GAE/g of date seeds. Extracts showed good radical scavenging activity in the different in vitro tests conducted in this work and were found to be effective in inhibiting the oxidation of cooked ground meat. Soluble phenolic extract from date seeds of sample 1 was most effective in inhibiting DNA strand scission by 74.15%, while soluble phenolics from palm leaves showed the highest inhibition of LDL cholesterol oxidation (87.82%). Both samples significantly inhibited mRNA level of COX-2 at concentrations as low as 5 μg/mL, with the most potent inhibitory effect being for date palm leaf extracts at 50 μg/mL. A number of phenolic compounds including proanthocyanidin dimers, catechin, epicatechin, 5-O-caffeoylshikimic acid isomers, ferulic acid, rutin and isorhamnetin hexoside, among others, were detected in date palm seeds and leaves.

scholarly journals Free Radical Scavenging and Antioxidant Potential of Ethanolic Leaf Extract of Diodia sarmentosa on High Fat Fed Wistar Rats

2020 ◽  
pp. 54-63
Author(s):  
Ezejiofor Tobias I. Ndubuisi ◽  
Korie Stephen Chinedumije ◽  
Peter Chukwudi
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Free Radical ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
High Fat ◽  
Ethanolic Leaf Extract

Diodia sarmentosa (Sw) commonly known as Zimbabwe flora or Tropical button weed is a straggling or procumbent perennial herb which grows in evergreen forest, riverine vegetation and bush land. It has been used traditionally for the treatment of ulcer, diabetes and other ailments. This study investigated the Free radical scavenging and antioxidant potential of ethanolic leaf extract of Diodia sarmentosa on high fat fed wistar rats. Thirty (30) male wistar rats (150 g-200 g) were divided into five (5) groups: Negative control rats, Positive control rats, Low dose extract rats treated with 250 mg/kg, High dose extract rats treated with 500mg/kg of the extract, and Standard antihyperlipidaemic drug rats treated with 5 mg/kg of Simvastatin. High fat diet was fed to the rats with Ghee and Coconut oil in the ratio of 3:1 for six (6) weeks, and administration of the treatments started from the 3rd week till the 6th week. The free radical scavenging and antioxidant potentials of Diodia sarmentosa was determined by assessing parameters like nitric oxide radical, hydroxyl radical, malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Glutathione S-transferase (GST) in the serum of the test rats. Diodia sarmentosa scavenged free radicals In vitro by inhibiting Nitric oxide and Hydroxyl radicals in a concentration-dependent manner, showing an inhibitory concentration at 50% (IC50) of 907.17 µg/ml ± 45.36 and 2173.44 µg/ml ± 100.11 respectively. Results obtained showed antioxidant enzymes like SOD, GPx and CAT in the serum of the test rats were significantly increased (p<0.05) by both doses of the extract when compared to the PC group and was similar to the standard drug used. GST activity was decreased in the serum by both doses of the extract, this decrease was not significant (p>0.05), while Malondialdehyde level was significantly decreased (p<0.05) by both doses of the extract in the serum of the tests rats. Diodia sarmentosa has antioxidants potentials because of its ability to scavenge free radicals, replenish antioxidant enzymes and reduce lipid peroxidation.

scholarly journals Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4800
Author(s):  
Ajit Kumar ◽  
Preeti Kaushik ◽  
Sandra Incerpi ◽  
Jens Z. Pedersen ◽  
Sanjay Goel ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Free Radical ◽  
Ellagic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cellular Systems ◽  
Ros Production ◽  
Microsomal Lipid Peroxidation

The purpose of this study was to examine the free radical scavenging and antioxidant activities of ellagic acid (EA) and ellagic acid peracetate (EAPA) by measuring their reactions with the radicals, 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl using EPR spectroscopy. We have also evaluated the influence of EA and EAPA on the ROS production in L-6 myoblasts and rat liver microsomal lipid peroxidation catalyzed by NADPH. The results obtained clearly indicated that EA has tremendous ability to scavenge free radicals, even at concentration of 1 µM. Interestingly even in the absence of esterase, EAPA, the acetylated product of EA, was also found to be a good scavenger but at a relatively slower rate. Kinetic studies revealed that both EA and EAPA have ability to scavenge free radicals at the concentrations of 1 µM over extended periods of time. In cellular systems, EA and EAPA were found to have similar potentials for the inhibition of ROS production in L-6 myoblasts and NADPH-dependent catalyzed microsomal lipid peroxidation.

Free radicals scavenging aptitude of Spilanthes acmella

2021 ◽  
pp. 2631-2634
Author(s):  
K. Gouri Sankar ◽  
B. S. Venkateswarlu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Spilanthes Acmella

Now a days, oxidative stress is one of the major disease around the world. The excess production of oxidants in the body are key source for development of different chronic diseases and are leading to damage the multiple organs' function, finally to cellular mortality. The natural remedy is the most important medicine to control the overproduction of oxidants and to obtain more antioxidants. Natural product have been using to treat diseases and as food supplements as nutrients. In this point of view, the current study was carried to evaluate the antioxidant activity of Spilanthes acmella root parts. The free radical scavenging activity was studied against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl free radicals using standard methods. The Spilanthes acmella root part extracts showed concentration dependent antioxidant activity on tested free radicals. The extracts showed modest effect on free radicals compared to the standard drug ascorbic acid and hydro-alcoholic extract possesses more activity than other two extracts. The end results of current study clearly state that Spilanthes acmella root possess free radical scavenging activity and also confirms its' traditional value in traditional medicine. The further research is worthful on isolation of different bioactive molecules and different biological activities evaluation on Spilanthes acmella.

Utilization Phase Transition Component Method to Prepare Specially Functionalized Nanoemulsion by Adding Resveratrol/Phenethyl Resorcinol Mixed Active Components and Application in Free Radicals Removal

2020 ◽  
Vol 20 (12) ◽  
pp. 7769-7774
Author(s):  
Wenwen Liu ◽  
Songzhu Cui ◽  
Jianhua Ji ◽  
Dejun Sun ◽  
Tao Wu
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Ionic Strength ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Mass Ratio ◽  
Hydrogen Binding ◽  
Active Components ◽  
Hydrophobic Binding ◽  
Radical Scavenging Ability

To explore the possibility of using a specially functionalized nanoemulsion for the removal of free radical, resveratrol and phenethyl resorcinol were employed to form resveratrol/phenethyl resorcinol mixed active components, and the removal efficiency of free radicals was evaluated. Original nanoemulsion and specially functionalized-nanoemulsion were characterized by DLS and EPR. The free radical scavenging ability of the mixed active components of resveratrol/phenethyl resorcinol was determined as a function of the mass ratio of resveratrol to phenethyl resorcinol, temperature, and ionic strength. In the results, the removal of free radicals by the resveratrol/phenethyl resorcinol nanoemulsion system was found to be more effective than the nanoemulsion alone. At a higher resveratrol ratio, more than 68% of free radicals could be removed. The efficiency was also found to increase with rising temperature. However, efficiency decreased with the increase in ionic strength. In conclusion, compared with conventional nanoemulsion, the combined utilization of nanoemulsion and the mixed active components of resveratrol/phenethyl resorcinol achieved better results for the removal of free radicals because of synergistic effect between nanoemulsion droplets and the mixed active components of resveratrol/phenethyl resorcinol, involving hydrophobic binding, hydrogen binding, and partitioning.

scholarly journals Isolation of bioactive compounds from Bergenia ciliata (haw.) Sternb rhizome and their antioxidant and anticholinesterase activities

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Roheena Zafar ◽  
Habib Ullah ◽  
Muhammad Zahoor ◽  
Abdul Sadiq
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenging ◽  
Total Phenolic

Abstract Background Bergenia ciliata is a medicinal plant used for the treatment of diarrhea, vomiting, fever, cough, diabetes, cancer, pulmonary disorders and wound healing. Methods In this study, Bergenia ciliata crude extract, subfractions, and isolated compounds were evaluated for their antioxidant and anticholinesterase potential. The free radical scavenging capacities of the extracts determined using DPPH and ABTS assays. The anticholinesterase potentials were determined using acetylcholine esterase and butyryl choline esterase enzymes. To determine the phytochemical composition, the extracts were subjected to HPLC analysis and silica gel column isolation. Based on HPLC fingerprinting results, the ethyl acetate fraction was found to have more bioactive compounds and was therefore subjected to silica gel column isolation. As a result, three compounds; pyrogallol, rutin, and morin were isolated in the pure state. The structures of the isolated compounds were elucidated using spectroscopic techniques like 1H-NMR, IR and UV-Visible. Results The crude extract showed maximum anticholinesterase (acetylcholinesterase = 90.22 ± 1.15% and butyrylcholinesterase = 88.22 ± 0.71%) and free radical scavenging (87.37 ± 2.45 and 83.50 ± 0.70% respectively against DPPH and ABTS radicals) potentials. The total phenolic contents (expressed as equivalent of gallic acid; mgGAE/g) were higher in ethyl acetate fraction (80.96 ± 1.74) followed by crude extract (70.65 ± 0.86) while the flavonoid contents (expressed as quercetin equivalent; mgQE/g) and were higher in crude extract (88.40 ± 1.12) followed by n-butanol fraction (60.10 ± 1.86). The isolated bioactive compounds pyrogallol, rutin, and morin were found active against ABTS and DPPH free radicals. Amongst them, pyrogallol was more active against both free radicals. Reasonable anticholinesterase activities were recorded for pyrogallol against selected enzymes. Conclusion The extracts and isolated compounds showed antioxidant and acetylcholinesterase inhibitory potentials. It was concluded that this plant could be helpful in the treatment of oxidative stress and neurological disorders if used in the form of extracts.

Sign in / Sign up

Export Citation Format

Share Document