scholarly journals Comparison of the performance of lipoperoxidation-antioxidant protection system in rats at different periods under immobilization stress effects

2021 ◽  
Author(s):  
Oralbek Z. Ilderbayev ◽  
Gulmira M. Zharmakhanova ◽  
Assem K. Okassova ◽  
Akmaral Zh. Nursafina ◽  
Gulzhan O. Ilderbayeva
Keyword(s):  
Immobilization Stress ◽  
Protection System ◽  
Antioxidant Protection ◽  
Stress Effects ◽  
Antioxidant Protection System

scholarly journals The state of antioxidant protection system in cows under the influence of heavy metals

2020 ◽  
Vol 11 (2) ◽  
pp. 237-242
Author(s):  
L. G. Slivinska ◽  
A. R. Shcherbatyy ◽  
B. O. Lukashchuk ◽  
B. V. Gutyj
Keyword(s):  
Heavy Metals ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Malonic Dialdehyde ◽  
Protection System ◽  
Lipid Hydroperoxides ◽  
Technogenic Pollution ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Antioxidant Protection System

A highly relevant problem of modern veterinary science is the study of features and mechanisms of combined action of the most common heavy metals – cadmium and plumbum and their influence on the body of humans and animals in the regions of Ukraine under technogenic pollution. The purpose of the work was to study the influence of heavy metals on the state of the antioxidant protection system of cows, in particular on the content of lipid peroxidation products (malonic dialdehyde, lipid hydroperoxides and diene conjugates), and activity of antioxidant enzymes (glutathione peroxidase and superoxide dismutase), depending on the distance to the heaps of mines in the coal basin. The study objects were cows of black-and-white breed at the age of 3–7 years. It was established that this parameter in the place with the highest concentration of diene conjugates in the blood of cows was by 25.8 % higher compared to the place of low concentration and 12.1 % higher compared to the place with medium concentration. In the place with the highest content of lipid hydroperoxides in the blood of cows the parameter was 23.7 % higher compared to the cows from the place with the low content. The concentration of lipid hydroperoxides in the blood of cows from the place with the medium content was 16.7% higher compared to the cows from the place with the low content. The parameter from the place with the lowest content of lipid hydroperoxides in the blood of cows was 12.1% lower compared to the place with the highest content. The level of malonic dialdehyde in the blood of cows from the technogenic pollution zone in the place with the largest amount was higher by 36.2; 34.0 and 18.8 % – compared to places with medium and low levels, respectively. The activity of superoxide dismutase in the blood of cows in the place with its highest activity was 0.284 ± 0.0099 % block. reac/g Hb, and in the place with the lowest activity – 0.23 ± 0.0051 % block. reac/g Hb. The activity of glutathione peroxidase in the blood of cows in farms of the technogenic pollution zone depended on the distance to the mine. These researches will further develop effective methods of treating cows under the influence of heavy metals, in particular regarding the antioxidant system.

scholarly journals Functional State of the Thyroid Gland and Lipid Peroxidation and Antioxidant Protection System in Women of Reproductive Age with Chronic Form of Parenteral Viral Hepatitis

2019 ◽  
Vol 4 (1) ◽  
pp. 31-35
Author(s):  
Maria A. Rashidova ◽  
Leonid F. Sholokhov ◽  
Marina A. Darenskaya ◽  
Lyubov I. Kolesnikova
Keyword(s):  
Lipid Peroxidation ◽  
Viral Hepatitis ◽  
Functional State ◽  
Chronic Viral Hepatitis ◽  
Protection System ◽  
Reproductive Age ◽  
Control Group ◽  
Antioxidant Protection ◽  
Chronic Form ◽  
Antioxidant Protection System

Background.Chronic viral hepatitis is a complex global problem and is still far from being solved. Many researchers point out influence of viral hepatitis on the reproductive system of women.Aim.To identify features of the functional state of pituitary-thyroid units of neuroendocrinal regulation, and to evaluate lipid peroxidation – antioxidant protection with determination of the oxidative stress coefficient in perimenopausal women with chronic form of hepatitis.Materials and methods. Study included 44 women with chronic viral hepatitis and 28 healthy women of the same age. Immunoabsorbent, spectrophotometric, fluorometric and statistical methods were used.Results.In patients with chronic viral hepatitis, we detected an increase in thyroxine levels – by 29.6% (р < 0.001) and free triiodothyronine values – by 65.7% (р = 0.008) in comparison with the control group. In the group with chronic viral hepatitis, the TBA-reactive products level increased by 1.9 times (р = 0.006), and superoxide dismutase activity decreased by 1.3 times (р < 0.001), total antioxidant activity – by 1.7 times (р < 0.001) and α-tocopherol level – by 1.3 times (р = 0.005) in comparison with the control group.Conclusions. The obtained data demonstrate the features of the pituitary-thyroid system functioning and the lipid peroxidation – antioxidant protection system in patients with chronic hepatitis. The chronic form of hepatitis is characterized by metabolic disorders that require a more careful approach in diagnosis and management.

scholarly journals State of the antioxidant protection system of rat liver in ischemia and reperfusion

2020 ◽  
Vol 24 (1) ◽  
pp. 93-104
Author(s):  
Konstantin Andreevich Popov ◽  
Ilia Mikhaylovich Bykov ◽  
Igor Yuryevich Tsymbalyuk ◽  
Yana Evgenievna Denisova ◽  
Anzhela Nikolaevna Stolyarova ◽  
...  
Keyword(s):  
Rat Liver ◽  
The State ◽  
Protection System ◽  
Cytosolic Fraction ◽  
Rat Liver Mitochondria ◽  
Ischemia And Reperfusion ◽  
Antioxidant Protection ◽  
Reperfusion Period ◽  
Mitochondrial Suspension ◽  
Antioxidant Protection System

Purpose: determination of the state of the antioxidant protection system of the cytosolic fraction and suspension of rat liver mitochondria after experimental ischemia and reperfusion. Materials and methods: the study was conducted using white mature rats, divided into 3 groups: the control group (n = 15); The 2nd group of animals (n = 15), from which the liver was taken after 15 minutes of liver ischemia; the 3rd group of rats (n = 15), from which the liver was taken after a 15-minute reperfusion period, followed by a 15-minute ischemic period. Mitochondrial suspension and cytosolic fraction were isolated from liver tissue. Results: the obtained research results showed the presence of certain pathobiochemical changes in the suspension of mitochondria and the cytosolic fraction after ischemia or reperfusion. In the mitochondrial suspension during the reperfusion period it was found an adaptive increase in the activity of glutathione peroxidase by 39% and glutathione reductase by 61%. In the cytosolic fraction, it was the most remarkable increase of the total antioxidant capacity by 38% already during ischemia and a progressive decrease in the level of reduced glutathione form by 26% in ischemic and 55% in reperfusion period. The change in the state of the antioxidant system occurred against the background of an increase in the number of products of oxidative modifications of biomolecules by 40% during ischemia and 2.2 times after reperfusion. Conclusion: The results indicate the need to develop not only a mitochondria-oriented correction of oxidative disorders, but also active support for the components of the cytosol, which provide the main accumulation of free radical damage products and their subsequent removal from the cell, which is essential for survival.

scholarly journals THE INHIBITING EFFECT OF FS-1 DRUG ON THE ANTIOXIDANT PROTECTION SYSTEM OF MYCOBACTERIA TUBERCULOSIS

2021 ◽  
Vol 2 (344) ◽  
pp. 64-73
Author(s):  
B. Kerimzhanova ◽  
A. Jumagaziyeva ◽  
N. Аkhatullina ◽  
Zh. Iskakbayeva ◽  
E. Sakhipov
Keyword(s):  
Protection System ◽  
Antioxidant Protection ◽  
Inhibiting Effect ◽  
Antioxidant Protection System ◽  
Mycobacteria Tuberculosis

scholarly journals The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

2019 ◽  
Vol 2 (3) ◽  
pp. 19-22
Author(s):  
L. Romanovych ◽  
B. Kurtyak ◽  
D. Mudrak
Keyword(s):  
Antioxidant Enzymes ◽  
Broiler Chickens ◽  
Protein Structures ◽  
Oxidative Modification ◽  
Protection System ◽  
Inhibitory Influence ◽  
Antioxidant Protection ◽  
Oxidative Modification Of Proteins ◽  
Vitamins E And C ◽  
Antioxidant Protection System

Chickens in the control group received a standard ration, the first experimental – ration with the addition of tocopherol acetate, the second experimental – ascorbic acid, and the third – a complex of these vitamins. On the basis of the conducted researches the influence of vitamin supplements on the indexes of oxidation intensity of lipid and protein structures was analyzed. The intensity of the processes of oxidative modification of proteins largely depends on the level of vitamins E and C in the ration of broiler chickens. Supplementation with vitamin C and with vitamin E resulted in an inhibitory influence on the content of OMP products, the level of which is largely regulated by the enzyme and non-enzymatic links of the antioxidant protection system. Searches have shown the stimulating influence of the addition of vitamins on the activity of antioxidant enzymes. In particular, SOD activity increased by 19 % on the 34th day, by 32 % on the 41st day, whereas glutathione peroxidase activity was 5.7 and 20.8 %, respectively, compared to the control chickens group. An increase in the activity of antioxidant enzymes occurred against the background of a decrease in the intensity of oxidation processes. At the same time, there was also an increase in the content of recovered glutathione in the blood of chickens of the second and third experimental groups at 34th and 41st days of age. Thereby, searches have shown that supplementing the ration with vitamins E and C with a positive influence on the organism's adaptive capacity and antioxidant potential, which is extremely important to ensure the metabolic homeostasis of chickens during critical periods of their cultivation.

THE ROLE OF THE GLUTATHIONE SYSTEM IN MAINTAINING REDOX-HOMEOSTASIS AND ANTIOXIDANT PROTECTION IN INFLAMMATORY AND DEGENERATIVE DISEASES OF THE ORGAN OF VISION

2021 ◽  
Vol 19 (4) ◽  
pp. 382-391
Author(s):  
V. G. Marmysh ◽  
Keyword(s):  
Oxidative Stress ◽  
Redox Homeostasis ◽  
The Body ◽  
Protection System ◽  
Antioxidant Protection ◽  
Degenerative Diseases ◽  
Glutathione System ◽  
Intracellular Glutathione ◽  
Antioxidant Protection System

Objective. To review and analyze literature data as well as evaluate the role of the glutathione antioxidant system in inflammatory and degenerative diseases of the organ of vision. Material and Methods. Multiple sources of foreign and domestic literature concerning the problem of oxidative stress, antioxidant protection system, glutathione and their role in inflammatory and degenerative diseases of the organ of vision were analyzed using main scientific and medical databases. More than 120 sources in the medical literature over the past 15 years were evaluated, 50 of them have been selected for final summary. Results. It was found that oxidative stress, based on free radical oxidation reactions, including LPO processes, plays a leading role in the development of inflammatory and degenerative diseases of the organ of vision. The key link in the eye’s antioxidant protection system is the glutathione system, which includes glutathione itself (GSH) and glutathionedependent enzymes: glutathione peroxidase (GPO), glutathione reductase (GR), glutathione-S-transferase (GST). Inflammatory and degenerative diseases of the eye are accompanied by reduction of the intracellular glutathione pool and imbalance between its reduced and oxidized forms (GSH / GSSG). Conclusions. Oxidative stress has a high correlation with inflammation and is the most important pathogenetic mechanism in inflammatory and degenerative diseases of the eye. The key role in antioxidant protection and maintenance of redox homeostasis of eye tissues belongs to the glutathione system, which includes GSH itself and glutathione-dependent enzymes (GPO, GR, GST). Inflammatory and degenerative processes in the eye are accompanied by decrease of the intracellular glutathione pool (GSH) and imbalance between its reduced and oxidized forms (GSH / GSSG). A shift in this balance significantly affects normal cell functioning, up to its apoptosis, and triggers multiple pathological conditions within the body, including diseases of the organ of vision.

scholarly journals Microbial preparations as a factor of plant resistance to the stress effects of heavy metals

2020 ◽  
Author(s):  
L.A. Chaikovskaya ◽  
◽  
M.I. Baranskaya ◽  
O.L. Ovsienko ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Ascorbic Acid ◽  
Winter Wheat ◽  
Soil Contamination ◽  
Plant Resistance ◽  
Antioxidant Protection ◽  
Stress Effects ◽  
Wheat Leaves ◽  
Antioxidant Protection System

The results of the influence of the complex microbial preparations (CMP) on the formation of the winter wheat antioxidant protection system in case of soil contamination with Pb, Cu, Cr are presented. Soil contamination with HM led to an increase in the content of ascorbic acid, glutathione, and peroxidase in wheat leaves. The use of CMP for pre-sowing inoculation of seeds helps to reduce the content of these indicators in the leaves of plants. Thus, bacterization contributed to a decrease in the content of ascorbic acid in the leaves by 13-35%. There was also a decrease in the content of glutathione in the leaves of plants grown from inoculated seeds. It decreased by 14-32%, 12-25%, and 19-24% on the background of НM soil contamination at the level of 1, 2.5 and 5 MPC, respectively. Our studies have shown a decrease in the content of peroxidase in the leaves of bacterized wheat plants on the background of soil contamination at the level of 1, 2.5 and 5 MPC HM by 7–19%, 14-33%, and 25-49%, respectively. Thus, soil contamination with HM leads to an increase in the content of ascorbic acid, glutathione, and peroxidase in the leaves of young winter wheat plants. The use of CMP for pre- sowing inoculation of seeds helps to reduce the content of these indicators in the phytomass of plants. This indicates an increased plant resistance to oxidative stress caused by HM.

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