scholarly journals Infertility in dairy cows – Possible bacterial and viral causes

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Attila Dobos ◽  
Levente Szeredi ◽  
Mislav Kovačić ◽  
Dražen Đuričić ◽  
István Kiss ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr ◽  
Bovine Viral Diarrhoea Virus ◽  
Statistical Correlation ◽  
Neutrophil Granulocytes ◽  
Histophilus Somni ◽  
Diarrhoea Virus ◽  
Histiocytic Infiltration

In this research uterine swab and biopsy samples were collected from 40 infertile dairy cows kept at five dairy cattle farms in Hungary. Samples were tested for bacteria including Coxiella burnetii chlamydiae, Mycoplasma and Ureaplasma, and for the viruses Bovine herpesvirus 1 (BoHV-1) and Bovine viral diarrhoea virus (BVDV). Chlamydiaceae DNA was detected by real-time PCR in 22/40 (55%) samples. Coxiella burnetii DNA was detected in 3/40 (7.5%) cases by real-time PCR. Mycoplasma and Ureaplasma DNA was found in 2/40 (5%) and 4/40 (10%) cows, respectively. BVD and BoHV-1 DNA was not detected in any samples. Escherichia coli as a recognised uterine pathogen was found in two cases. The following potential uterine pathogens were found: Bacillus licheniformis (one case), non- haemolytic streptococci (five cases), Histophilus somni (two cases) and Candida krusei (two cases). Blood samples were collected at same time as swab samples from all 40 cows, and their examination for C. burnetii antibodies by ELISA revealed seropositivity in 26/40 cows (65%). Histological examination of the uterine biopsy samples showed the presence of mild lympho-histiocytic infiltration in the mucosain 22 cases (59%). Moderatelympho-histiocytic infiltration of the endometrium was evident in 13 cases (35%), while in two cases (6%) severe inflammatory cell infiltration of the endometrium with lympho-histiocytes and neutrophil granulocytes was found. Although no statistical correlation could be demonstrated between the severity of histological lesions of the endometrium and the uterine pathogenicity of the bacteria (P = 0.8555), endometritis of a certain severity grade and/or a recognised or potential uterine pathogen were found in all samples. The latter may play a role in the development of infertility either collectively or independently.

scholarly journals Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 674 ◽  
Author(s):  
Andrew J. Read ◽  
Sarah Gestier ◽  
Kate Parrish ◽  
Deborah S. Finlaison ◽  
Xingnian Gu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reproductive Tract ◽  
Bovine Viral Diarrhoea Virus ◽  
Neutralizing Antibody ◽  
Bovine Viral Diarrhoea ◽  
Artificial Breeding ◽  
Diarrhoea Virus ◽  
Antibody Titres ◽  
Low Levels

Infection of bulls with bovine viral diarrhoea virus (BVDV) can result in the development of virus persistence, confined to the reproductive tract. These bulls develop a normal immune response with high neutralizing antibody titres. However, BVDV can be excreted in the semen for a prolonged period. Although relatively rare, in this study we describe six separate cases in bulls being prepared for admission to artificial breeding centres. Semen samples were tested in a pan-Pestivirus-reactive real-time PCR assay and viral RNA was detected in semen from five of the bulls for three to eight months after infection. In one bull, virus was detected at low levels for more than five years. This bull was found to have one small testis. When slaughtered, virus was only detected in the abnormal testis. The low levels of BVDV in the semen of these bulls were only intermittently detected by virus isolation in cell culture. This virus-contaminated semen presents a biosecurity risk and confirms the need to screen all batches of semen from bulls that have been previously infected with BVDV. The use of real-time PCR is recommended as the preferred laboratory assay for this purpose.

The problem of bovine histophilosis and Histophilus somni DNA detection by real-time PCR

2020 ◽  
Vol 23 (11) ◽  
pp. 28-33
Author(s):  
S.P. Yatsentyuk ◽  
◽  
D.A. Rudnyayev ◽  
Yu.I. Pobolelova ◽  
M.S. Krasnikova ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Histophilus Somni

Measurement of the antibiotic susceptibility of Coxiella burnetii using real time PCR

2004 ◽  
Vol 23 (2) ◽  
pp. 169-174 ◽  
Author(s):  
Areen Boulos ◽  
Jean-Marc Rolain ◽  
Max Maurin ◽  
Didier Raoult
Keyword(s):  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr ◽  
Antibiotic Susceptibility

scholarly journals Estimating Clinically Relevant Cut-Off Values for a High-Throughput Quantitative Real-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle

2021 ◽  
Vol 8 ◽  
Author(s):  
Alicia F. Klompmaker ◽  
Maria Brydensholt ◽  
Anne Marie Michelsen ◽  
Matthew J. Denwood ◽  
Carsten T. Kirkeby ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Pasteurella Multocida ◽  
Age Groups ◽  
Therapeutic Interventions ◽  
Respiratory Pathogens ◽  
Logistic Regression Models ◽  
Histophilus Somni ◽  
Nasal Swabs

Bovine respiratory disease (BRD) results from interactions between pathogens, environmental stressors, and host factors. Obtaining a diagnosis of the causal pathogens is challenging but the use of high-throughput real-time PCR (rtPCR) may help target preventive and therapeutic interventions. The aim of this study was to improve the interpretation of rtPCR results by analysing their associations with clinical observations. The objective was to develop and illustrate a field-data driven statistical method to guide the selection of relevant quantification cycle cut-off values for pathogens associated with BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based on nasal swabs from calves. We used data from 36 herds enrolled in a Danish field study where 340 calves within pre-determined age-groups were subject to clinical examination and nasal swabs up to four times. The samples were analysed with the rtPCR system. Each of the 1,025 observation units were classified as sick with BRD or healthy, based on clinical scores. The optimal rtPCR results to predict BRD were investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by interpreting scatterplots and results of mixed effects logistic regression models. The clinically relevant rtPCR cut-off suggested for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, while no cut-off could be determined for M. haemolytica and T. pyogenes. The demonstrated approach can provide objective support in the choice of clinically relevant cut-offs. However, for robust performance of the regression model sufficient amounts of suitable data are required.

Evaluation of a Real-Time PCR Assay to Detect Coxiella burnetii

2006 ◽  
Vol 1078 (1) ◽  
pp. 563-565 ◽  
Author(s):  
S. R KLEE ◽  
H. ELLERBROK ◽  
J. TYCZKA ◽  
T. FRANZ ◽  
B. APPEL
Keyword(s):  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr ◽  
Pcr Assay

Detection of Coxiella burnetii in placenta and abortion samples from British ruminants using real-time PCR

2010 ◽  
Vol 167 (25) ◽  
pp. 965-967 ◽  
Author(s):  
R. M. Jones ◽  
D. F. Twomey ◽  
S. Hannon ◽  
J. Errington ◽  
G. C. Pritchard ◽  
...  
Keyword(s):  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr

scholarly journals Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Ashraf Mohabati Mobarez ◽  
Ehsan Mostafavi ◽  
Mohammad Khalili ◽  
Saber Esmaeili
Keyword(s):  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr ◽  
Q Fever ◽  
Goat Milk ◽  
Raw Milk ◽  
Molecular Evidence ◽  
Milk Samples ◽  
Sheep Milk ◽  
Dairy Animals

Coxiella burnetii is the causative agent of Q fever in humans and animals. This study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. In this study, 162 individual milk samples were collected from 43 farms in three provinces (Tehran, Hamadan, and Mazandaran). Real-time PCR was used for the detection of IS1111a element of C. burnetii. In total, 23 of 162 samples (14.2%, 95% confidence interval (CI): 9.65–20.2%) were positive for C. burnetii by real-time PCR. C. burnetii was detected in 10.17% (95% CI: 4.74–20.46) of goat milk samples. In sheep milk samples, 18.6% (95% CI: 9.74–32.62) were positive, and C. burnetii was detected in 15% (95% CI: 8.1–26.11) of cattle milk samples. Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. These findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran.

Prevalence of Neospora caninum and bovine viral diarrhoea virus in dairy cows in Southern Vietnam

2008 ◽  
Vol 175 (3) ◽  
pp. 390-394 ◽  
Author(s):  
Mai Chi Duong ◽  
Stefan Alenius ◽  
Lam Thi Thu Huong ◽  
Camilla Björkman
Keyword(s):  
Dairy Cows ◽  
Neospora Caninum ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Southern Vietnam ◽  
Diarrhoea Virus
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